scholarly journals Termination of cAMP signals by Ca2+ and Gαi via extracellular Ca2+ sensors

2005 ◽  
Vol 171 (2) ◽  
pp. 303-312 ◽  
Author(s):  
Andrea Gerbino ◽  
Warren C. Ruder ◽  
Silvana Curci ◽  
Tullio Pozzan ◽  
Manuela Zaccolo ◽  
...  
Keyword(s):  
Resonance Energy Transfer ◽  
Low Frequency ◽  
Resonance Energy ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Hek 293 ◽  
Long Duration ◽  
Low Pass

Termination of cyclic adenosine monophosphate (cAMP) signaling via the extracellular Ca2+-sensing receptor (CaR) was visualized in single CaR-expressing human embryonic kidney (HEK) 293 cells using ratiometric fluorescence resonance energy transfer–dependent cAMP sensors based on protein kinase A and Epac. Stimulation of CaR rapidly reversed or prevented agonist-stimulated elevation of cAMP through a dual mechanism involving pertussis toxin–sensitive Gαi and the CaR-stimulated increase in intracellular [Ca2+]. In parallel measurements with fura-2, CaR activation elicited robust Ca2+ oscillations that increased in frequency in the presence of cAMP, eventually fusing into a sustained plateau. Considering the Ca2+ sensitivity of cAMP accumulation in these cells, lack of oscillations in [cAMP] during the initial phases of CaR stimulation was puzzling. Additional experiments showed that low-frequency, long-duration Ca2+ oscillations generated a dynamic staircase pattern in [cAMP], whereas higher frequency spiking had no effect. Our data suggest that the cAMP machinery in HEK cells acts as a low-pass filter disregarding the relatively rapid Ca2+ spiking stimulated by Ca2+-mobilizing agonists under physiological conditions.

scholarly journals Cardiac Hypertrophy Changes Compartmentation of cAMP in Non-Raft Membrane Microdomains

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 535
Author(s):  
Nikoleta Pavlaki ◽  
Kirstie A. De Jong ◽  
Birgit Geertz ◽  
Viacheslav O. Nikolaev ◽  
Alexander Froese
Keyword(s):  
Cardiac Hypertrophy ◽  
Ventricular Myocytes ◽  
Resonance Energy Transfer ◽  
Pressure Overload ◽  
Resonance Energy ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Differential Regulation ◽  
Membrane Microdomains ◽  
Transgenic Mouse Line

3′,5′-Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger which plays critical roles in cardiac function and disease. In adult mouse ventricular myocytes (AMVMs), several distinct functionally relevant microdomains with tightly compartmentalized cAMP signaling have been described. At least two types of microdomains reside in AMVM plasma membrane which are associated with caveolin-rich raft and non-raft sarcolemma, each with distinct cAMP dynamics and their differential regulation by receptors and cAMP degrading enzymes phosphodiesterases (PDEs). However, it is still unclear how cardiac disease such as hypertrophy leading to heart failure affects cAMP signals specifically in the non-raft membrane microdomains. To answer this question, we generated a novel transgenic mouse line expressing a highly sensitive Förster resonance energy transfer (FRET)-based biosensor E1-CAAX targeted to non-lipid raft membrane microdomains of AMVMs and subjected these mice to pressure overload induced cardiac hypertrophy. We could detect specific changes in PDE3-dependent compartmentation of β-adrenergic receptor induced cAMP in non-raft membrane microdomains which were clearly different from those occurring in caveolin-rich sarcolemma. This indicates differential regulation and distinct responses of these membrane microdomains to cardiac remodeling.

scholarly journals Heart failure leads to altered β2-adrenoceptor/cyclic adenosine monophosphate dynamics in the sarcolemmal phospholemman/Na,K ATPase microdomain

2018 ◽  
Vol 115 (3) ◽  
pp. 546-555 ◽  
Author(s):  
Zeynep Bastug-Özel ◽  
Peter T Wright ◽  
Axel E Kraft ◽  
Davor Pavlovic ◽  
Jacqueline Howie ◽  
...  
Keyword(s):  
Heart Failure ◽  
Ventricular Myocytes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Adult Rat ◽  
C Terminus ◽  
Complex Forms ◽  
Cardiac Excitation

Abstract Aims Cyclic adenosine monophosphate (cAMP) regulates cardiac excitation–contraction coupling by acting in microdomains associated with sarcolemmal ion channels. However, local real time cAMP dynamics in such microdomains has not been visualized before. We sought to directly monitor cAMP in a microdomain formed around sodium–potassium ATPase (NKA) in healthy and failing cardiomyocytes and to better understand alterations of cAMP compartmentation in heart failure. Methods and results A novel Förster resonance energy transfer (FRET)-based biosensor termed phospholemman (PLM)-Epac1 was developed by fusing a highly sensitive cAMP sensor Epac1-camps to the C-terminus of PLM. Live cell imaging in PLM-Epac1 and Epac1-camps expressing adult rat ventricular myocytes revealed extensive regulation of NKA/PLM microdomain-associated cAMP levels by β2-adrenoceptors (β2-ARs). Local cAMP pools stimulated by these receptors were tightly controlled by phosphodiesterase (PDE) type 3. In chronic heart failure following myocardial infarction, dramatic reduction of the microdomain-specific β2-AR/cAMP signals and β2-AR dependent PLM phosphorylation was accompanied by a pronounced loss of local PDE3 and an increase in PDE2 effects. Conclusions NKA/PLM complex forms a distinct cAMP microdomain which is directly regulated by β2-ARs and is under predominant control by PDE3. In heart failure, local changes in PDE repertoire result in blunted β2-AR signalling to cAMP in the vicinity of PLM.

A New Capacitance Multiplier Structure with High Multiplication Factor for Ultra-Low-Frequency Filter in Biomedical Applications

2019 ◽  
Vol 29 (07) ◽  
pp. 2050109
Author(s):  
Yan Li ◽  
Yong Liang Li
Keyword(s):  
Biomedical Applications ◽  
Low Frequency ◽  
Multiplication Factor ◽  
Frequency Filter ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Physiological Signal ◽  
Wide Range ◽  
Low Pass ◽  
Capacitance Multiplier

A novel capacitance multiplier is proposed to implement an ultra-low-frequency filter for physiological signal processing in biomedical applications. With the proposed multiplier, a simple first-order low-pass filter achieves a [Formula: see text]3-dB frequency of 33.4[Formula: see text]μHz with a 1-pF capacitance and a 20[Formula: see text]k[Formula: see text] resistance. This corresponds to a multiplication factor of as large as [Formula: see text]. By changing the controlling terminal, the [Formula: see text]3-dB frequency can be tuned in a wide range of 33.4[Formula: see text]μHz–6.3[Formula: see text]kHz.

Design of the Broadband Standard Signal Generator for Laser Locating System of the Mine Hoisting Container

2013 ◽  
Vol 427-429 ◽  
pp. 2033-2036
Author(s):  
Di Fan ◽  
Yan Gao ◽  
Yue Zhao
Keyword(s):  
High Frequency ◽  
Low Frequency ◽  
Frequency Standard ◽  
Analog Filters ◽  
Laser Ranging ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Noise Disturbance ◽  
Standard Signal ◽  
Low Pass

As the key junction between the ground and underground, hoisting systems as well as mines themselves are of vital importance to coalmine production. Laser ranging method is studied as a new solution of getting the real-time position directly. Furthermore, multi-scale phase based laser ranging principles are utilized in the system. The paper is aimed to conduct research into the problems existing in standard signal generating while using laser to locating the hoisting container, and to design standard sine generator circuits with DDS technology and DDS devices AD9850 to generate multiple frequency standard signals. In view of the serious noise disturbance in high frequency output, 4-order Chebyshev low-pass filter is designed, by using the integrated analog filters LT 6600-15, to filter the sine signals from AD9850 and to effectively weaken the noise disturbance. The established practical circuits are tested, obtaining trillion level high frequency and low frequency sine signals and fulfilling the requirements for the location system of hoisting containers.

scholarly journals Inductanceless high order low frequency filters for medical applications

2022 ◽  
Vol 12 (2) ◽  
pp. 1299
Author(s):  
Noor Thamer Almalah ◽  
Faris Hasan Aldabbagh
Keyword(s):  
Integrated Circuit ◽  
Low Frequency ◽  
Negative Resistance ◽  
Acceptable Result ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Transconductance Amplifier ◽  
Low Pass ◽  
Minimum Number ◽  
Order Butterworth Filter

<p>In this paper, a designed circuit used for low-frequency filters is implemented and realized the filter is based on frequency-dependent negative resistance (FDNR) as an inductor simulator to substitute the traditional inductance, which is heavy and high cost due to the coil material manufacturing and size area. The simulator is based on an active operation amplifier or operation transconductance amplifier (OTA) that is easy to build in an integrated circuit with a minimum number of components. The third and higher-order Butterworth filter is simulated at low frequency for low pass filter to use in medical instruments and low-frequency applications. The designed circuit is compared with the traditional proportional integral controller enhanced (PIE) and T section ordinary filter. The results with magnitude and phase response were compared and an acceptable result is obtained. The filter can be used for general applications such as medical and other low-frequency filters needed.</p>

TECHNIQUE OF APPLICATION FOR PROCESSING OF LOW PASS FILTER LF SIGNALS

10.12737/7905 ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 35-38
Author(s):  
Табаков ◽  
Yu. Tabakov ◽  
Лавлинский ◽  
V. Lavlinskiy
Keyword(s):  
Mathematical Model ◽  
Block Diagram ◽  
Low Frequency ◽  
Frequency Signal ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Low Pass ◽  
The Mathematical Model ◽  
40 Hz

The article includes a block diagram and the mathematical model for low pass filter designed for processing low frequency signal with a frequency of 10-40 Hz in order of eliminate various noises and interference.

Acoustic Perturbation Effects on the Fluid Dynamics and Swirling Flame Response in a Non-Premixed Co-Flow Burner

2010 ◽  
Author(s):  
Uyi Idahosa ◽  
Abhishek Saha ◽  
Navid Khatami ◽  
Chengying Xu ◽  
Saptarshi Basu
Keyword(s):  
Heat Release ◽  
Low Frequency ◽  
Ccd Camera ◽  
Pass Filter ◽  
Low Pass Filter ◽  
Fuel Flow ◽  
Flame Dynamics ◽  
Flame Response ◽  
Low Pass ◽  
Swirling Flame

An investigation into the response of non-premixed swirling flames to acoustic perturbations at various frequencies (fp = 0–315 Hz) and swirl intensities (S = 0.09 and 0.34) is carried out. Perturbations are generated using a loudspeaker at the base of an atmospheric co-flow burner with resulting velocity oscillation amplitudes |u′/Uavg| in the 0.03–0.30 range. The dependence of flame dynamics on the relative richness of the flame is investigated by studying various constant fuel flow rate flame configurations. Flame heat release is quantitatively measured and simultaneously imaged using a photomultiplier (PMT) and a phase-locked CCD camera. Both of which are fitted with 430nm bandpass filters for observing CH*chemiluminescence. The flame response is observed to exhibit a low-pass filter characteristic with minimal flame response beyond pulsing frequencies of 200Hz. Flames at lower fuel flow rates are observed to remain attached to the central fuel pipe at all acoustic pulsing frequencies. PIV imaging of the associated isothermal fields show the amplification in flame aspect ratio is caused by the narrowing of the inner recirculation zone (IRZ). The Rayleigh criterion (R) is used to assess the potential for instability of specific perturbation configurations and is found to be a good predictor of unstable modes. Phase conditioned analysis of the flame dynamics yield additional criteria in highly responsive modes to include the effective amplitude of velocity oscillations induced by the acoustic pulsing. Highly amplified responses were observed in pulsed flame configurations with Strouhal numbers (St = fpUavg/dm) in the 1–3.5 range. Heat release to velocity perturbation time delays on the order of the acoustic pulsing period also characterized the highly responsive flames. Finally, wavelet analyses of heat release perturbations indicate sustained low frequency oscillations that become more prominent for low acoustic pulsing frequencies in lean flame configurations.

scholarly journals Dynamics of Excitation and Inhibition in the Light-Adapted Limulus Eye in situ

1971 ◽  
Vol 58 (1) ◽  
pp. 1-19 ◽  
Author(s):  
Marguerite Biederman-Thorson ◽  
John Thorson
Keyword(s):  
Steady State ◽  
Lateral Inhibition ◽  
Low Frequency ◽  
Dynamic Responses ◽  
Relaxation Processes ◽  
Pass Filter ◽  
Generator Potential ◽  
Low Pass Filter ◽  
Low Pass

The dynamics of spike discharge in eccentric cell axons from the in situ lateral eye of Limulus, under small sinusoidal modulation of light to which the eye is adapted, are described over two decades of light intensity and nearly three decades of frequency. Steady-state lateral inhibition coefficients, derived from the very low-frequency response, average 0.04 at three interommatidial spacings. The gain vs. frequency of a singly illuminated ommatidium is described closely from 0.004 to 0.4 cps by the linear transfer function s0.25; this function also accounts approximately for the measured phase leads, the small signal adaptation following small step inputs, and for Pinter's (1966) earlier low-frequency generator potential data. We suggest that such dynamics could arise from a summation in the generator potential of distributed intensity-dependent relaxation processes along the dendrite and rhabdome. Analysis of the dynamic responses of an eccentric cell with and without simultaneously modulated illumination of particular neighbors indicates an effect equivalent to self-inhibition acting via a first-order low-pass filter with time constant 0.42 sec, and steady-state gain near 4.0. The corresponding filters for lateral inhibition required time constants from 0.35 to 1 sec and effective finite delay of 50–90 msec.

scholarly journals BacMam System for FRET-Based cAMP Sensor Expression in Studies of Melanocortin MC1 Receptor Activation

2012 ◽  
Vol 17 (8) ◽  
pp. 1096-1101 ◽  
Author(s):  
Olga Mazina ◽  
Reet Reinart-Okugbeni ◽  
Sergei Kopanchuk ◽  
Ago Rinken
Keyword(s):  
High Throughput Screening ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cyclic Adenosine Monophosphate ◽  
Second Messenger ◽  
Adenosine Monophosphate ◽  
Receptor Activation ◽  
Dependent Manner ◽  
Endogenous Expression ◽  
Mammalian Cell Lines

Cyclic adenosine monophosphate (cAMP) is a second messenger of many G-protein-coupled receptors (GPCRs) and a useful readout molecule to estimate the biological activity of various GPCR-specific agents. Here we report the development and use of a Förster resonance energy transfer (FRET) biosensor for cAMP (Epac2-camps) combined with a baculovirus-based BacMam transduction system. The constructed BacMam-Epac2-camps viral transduction system is a simple and robust tool for ligand screening at the second-messenger level in a variety of mammalian cell lines. The level of biosensor protein expression can easily be adjusted in a dose-dependent manner depending on the multiplicity of viral infection. For setting up the assay, we used a B16F10 murine melanoma cell line with endogenous expression of melanocortin-1 receptor (MC1R). The receptor activation was characterized by a set of MC1R full and partial agonists. Bivalent ions Ca2+ as well as Mg2+ modulated ligand potencies, whereas the effect was ligand and ion specific. Results obtained for MC1R indicate that the BacMam-Epac2-camps system may also be applicable for studying the activation of other GPCRs and may be implemented in routine analysis as well as in high-throughput screening.

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