scholarly journals A FINE STRUCTURAL STUDY OF ADHESIVE CELL JUNCTIONS IN HETEROTYPIC CELL AGGREGATES

1970 ◽  
Vol 47 (1) ◽  
pp. 197-210 ◽  
Author(s):  
Peter B. Armstrong
Keyword(s):  
Tissue Culture ◽  
Electron Microscope ◽  
Cell Sorting ◽  
Chicken Embryo ◽  
Cell Junctions ◽  
Cell Contact ◽  
Cell Aggregates ◽  
Undifferentiated Cell ◽  
Cell Type ◽  
Embryo Heart

Mixed suspensions of cells obtained by dissociation of 7 day chicken embryo heart and pigmented retina were allowed to reaggregate in tissue culture. The reaggregates which resulted contained both kinds of cells. Establishment of homogeneous tissues by cell sorting out in these reaggregates was advanced by 20 hr in culture and was complete within 2 days. When sorting out was advanced, heterotypic aggregates were fixed, sectioned, and examined in the electron microscope. Particular attention was paid to the morphology of regions of contact between cells. No qualitative differences were observed in the contact junctions between like cells (heart-heart or pigmented retina-pigment retina junctions) and unlike cells (heart-pigmented retina junctions). Broad areas of undifferentiated cell contact with cell membranes separated by a 100–200 A gap were formed regardless of cell type. Specialized junctions of the fascia and macula adherens type were also present, not only between like cells but also between unlike cells.

Contracting chicken embryo heart cells in tissue culture

1965 ◽  
Vol 70 (1) ◽  
pp. 94-109 ◽  
Author(s):  
George A. Pankey ◽  
William J. Mogabgab
Keyword(s):  
Tissue Culture ◽  
Chicken Embryo ◽  
Heart Cells ◽  
Embryo Heart

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Analysis of the effect of DEK overexpression on the survival and proliferation of bone marrow stromal cells

2019 ◽  
Vol 44 (4) ◽  
pp. 510-516
Author(s):  
Türkan Çakar ◽  
Ayten Kandilci
Keyword(s):  
Cell Lines ◽  
Stromal Cells ◽  
Cell Sorting ◽  
Bone Marrow Stromal Cells ◽  
Nuclear Protein ◽  
Cell Type ◽  
Facs Analysis ◽  
Periodic Growth ◽  
Stromal Cell Line ◽  
Rpmi 8226

Abstract Objective DEK is ubiquitously expressed and encodes a nuclear protein, which is also released from some cells. Overexpression of DEK suppresses proliferation of some blood cell progenitors whereas it increases proliferation of epithelial tumors. We showed that DEK is overexpressed in BM cells of 12% of multiple myeloma (MM) patients. Here, we aimed to test if DEK overexpression effects the proliferation and viability of BM stromal cells or MM cells co-cultured with DEK-overexpressing stromal cells, mimicking the BM microenvironment. Methods DEK is stably overexpressed in the BM stromal cell line HS27A. Periodic growth curve and fluorescent activated cell sorting (FACS) analysis was performed to determine the effect of DEK overexpression on HS27A cells and MM cell lines (RPMI-8226 and U266) that are co-cultured with these HS27A cells. Results We showed that, on the contrary to blood progenitors or ephitelial cells, DEK overexpression doesn’t alter the viability or proliferation of the HS27A cells, or the MM cell lines which are co-cultured with DEK-overexpressing HS27A cells. Conclusions Our results suggest that effect of DEK overexpression on the proliferation is cell type and context dependent and increased DEK expression is tolerable by the stromal cells and the co-cultured MM cell lines without effecting proliferation and viability.

An Inverse Relation Between Cell Density and the Number of Microvilli in Cultures of BHK 21 Hamster Fibroblasts

1970 ◽  
Vol 7 (3) ◽  
pp. 695-709
Author(s):  
C. H. O'NEILL ◽  
E. A. C. FOLLETT
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Monolayer Culture ◽  
Cell Contact ◽  
Inverse Relation ◽  
Culture Density ◽  
Microscope Technique ◽  
The Relationship ◽  
Number Of Cells ◽  
Scanning Electron

The relationship between culture density and the number of microvilli on the cell surface has been studied using BHK21 cells in established monolayer culture. The number of microvilli can be estimated quantitatively by a scanning electron-microscope technique. It can be increased by applying specific antiserum, but whether antibody is applied or not a significant number of cells regularly bear microvilli. This number is characteristic of the culture density. In sparse cultures above a certain minimum density it is relatively high, and in confluent cultures it is much reduced. These results indicate that microvilli may be inhibited by cell contact.

FURTHER STUDIES ON THE DUAL REQUIREMENT FOR PHENYLALANINE AND TYROSINE IN TISSUE CULTURE

1961 ◽  
Vol 39 (5) ◽  
pp. 925-932 ◽  
Author(s):  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Chick Embryo ◽  
Heart Cells ◽  
Present System ◽  
Chick Heart ◽  
High Concentrations ◽  
Chick Embryo Heart ◽  
Embryo Heart ◽  
Related Compounds

Seventeen structurally related compounds were tested for their ability to substitute for phenylalanine or tyrosine in the nutrition of chick embryo heart fragments. DL-Alanyl-DL-phenylalanine replaced phenylalanine. All other compounds had negligible effects, and most were toxic at high concentrations. β-Phenylserine, a phenylalanine antagonist, actually prolonged the survival of chick heart cells but only if both phenylalanine and tyrosine were present. Similarly, optimal reversal of β-phenylserine toxicity was dependent on the presence of both amino acids. Although phenylalanine and tyrosine are not interconvertible in the present system, it has been shown that three phenylalanine antagonists, p-fluorophenylalanine, β-2-thienylalanine, and β-phenylserine, can be identified by their relationship to tyrosine, rather than to phenylalanine.

Metabolic Co-Operation Between Biochemically Marked Mammalian Cells in Tissue Culture

1969 ◽  
Vol 4 (2) ◽  
pp. 353-367
Author(s):  
H. SUBAK-SHARPE ◽  
R. R. BÜRK ◽  
J. D. PITTS
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Mixed Culture ◽  
Molecular Basis ◽  
Mammalian Cells ◽  
Genetic Variant ◽  
Cell Contact ◽  
Metabolic Function ◽  
Indirect Contact ◽  
Fibroblast Line

Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity (IPP- cells) and therefore cannot normally incorporate [3H]hypoxanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP+ cells). If not in contact with IPP+ cells, IPP- cells do not incorporate added [3H]hypoxanthine into nucleic acid. IPP+ cells always do incorporate [3H]hypoxanthine and IPP- cells when in direct or indirect contact with IPP+ cells also incorporate the isotope. Cell to cell contact appears to be essential for this gain of a metabolic function by IPP- cells. The possible molecular basis and general implications of the phenomenon are discussed.

Familial acromegaly

1989 ◽  
Vol 121 (2) ◽  
pp. 286-289 ◽  
Author(s):  
Richard G. Pestell ◽  
Frank P. Alford ◽  
James D. Best
Keyword(s):  
Pituitary Adenomas ◽  
Multiple Endocrine Neoplasia ◽  
Histological Finding ◽  
Undifferentiated Cell ◽  
Prominent Feature ◽  
Cell Type ◽  
Mixed Cell ◽  
Endocrine Neoplasia ◽  
Low Incidence

Abstract. Five family members over 3 generations had isolated functional pituitary adenomas diagnosed. In four cases acromegaly was diagnosed, and in the fifth galactorrhoea from prolactin excess was the presenting feature. A prominent feature of the affected members tumours were histological finding of either atypical mixed cell or undifferentiated cell type. Given the low incidence of acromegaly and the demonstration of vertical transmission over 3 generations it is suggested this family represents an inherited pituitary syndrome, distinct from Multiple Endocrine Neoplasia (MEN) type 1.

scholarly journals In Vitro Activation of Feline Immunodeficiency Virus in Ramified Microglial Cells from Asymptomatically Infected Cats

2001 ◽  
Vol 75 (17) ◽  
pp. 8090-8095 ◽  
Author(s):  
Andreas Hein ◽  
Jean-Pierre Martin ◽  
Rüdiger Dörries
Keyword(s):  
Central Nervous System ◽  
Tissue Culture ◽  
Nervous System ◽  
Feline Immunodeficiency Virus ◽  
Mononuclear Cells ◽  
Central Nervous System Infection ◽  
Microglial Cells ◽  
Cell Contact ◽  
Immunodeficiency Virus

ABSTRACT Intravenous infection of cats with feline immunodeficiency virus was used as a model system to study activation of virus replication in brain-resident microglial cells in vitro. Virus release by ramified microglial cells isolated from subclinically infected animals was detectable in cell-free tissue culture supernatant only by reverse transcription and nested PCR of gag-specific RNA sequences and not by virion-associated reverse transcriptase activity. In contrast, cocultivation of in vivo-infected microglial cells with mitogen-activated peripheral blood mononuclear cells (PBMC) regularly allows detection of high virus yields in cell-free tissue culture fluid. Besides uptake and multiplication of microglia-derived virus in PBMC, release of virus from microglia is stimulated by cell contact with PBMC. The data suggest that T lymphocytes patrolling the central nervous system could reactivate the semilatent state of lentiviruses in microglial cells in the course of clinically silent central nervous system infection.

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