Macronuclear Duplication in the Ciliated Protozoan Euplotes

The ribbon-like macronucleus of Euplotes eurystomus pinches in half amitotically at each cell division. Several hours before the actual division two lightly staining duplication bands (reorganization bands) appear at the ends of the nucleus and approach each other slowly, finally meeting near the middle. Distal to the bands, that is, in regions through which the bands have already passed, the concentration of DNA (Feulgen) and "histone" (alkaline fast green) is greater than in the central zone. These facts suggest the hypothesis that DNA-histone synthesis takes place in a sequential fashion starting at the tips of the nucleus and proceeding to the middle. That this hypothesis is correct is shown by autoradiographic and photometric observations. Tritium-labelled thymidine is incorporated only in a limited region immediately distal to the bands. The average amount of Feulgen dye bound by the nucleus rises as the duplication bands approach each other, and is double the presynthesis value by the time the bands meet. A similar rise in the alkaline fast green dye is seen in duplicating nuclei, although no completely post-synthesis values were obtained in this study. The quantitative data are consistent with the assumption that the macronucleus contains a number of DNA-histone "units," presumably chromosomes, each of which duplicates once and only once.

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Histochemical Detection of Collagen Fibers by Sirius Red/Fast Green Is More Sensitive than van Gieson or Sirius Red Alone in Normal and Inflamed Rat Colon

PLoS ONE ◽

10.1371/journal.pone.0144630 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0144630 ◽

Cited By ~ 45

Author(s):

Cristina Segnani ◽

Chiara Ippolito ◽

Luca Antonioli ◽

Carolina Pellegrini ◽

Corrado Blandizzi ◽

...

Keyword(s):

Collagen Fibers ◽

Rat Colon ◽

Fast Green ◽

Green Is ◽

Histochemical Detection ◽

Sirius Red

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NUCLEIC ACID AND PROTEIN METABOLISM DURING THE MITOTIC CYCLE IN VICIA FABA

The Journal of Cell Biology ◽

10.1083/jcb.9.2.445 ◽

1961 ◽

Vol 9 (2) ◽

pp. 445-462 ◽

Cited By ~ 128

Author(s):

John Woodard ◽

Ellen Rasch ◽

Hewson Swift

Keyword(s):

Vicia Faba ◽

Total Protein ◽

Mitotic Cycle ◽

Mitotic Division ◽

Fast Green ◽

Cytoplasmic Rna ◽

Minor Peak ◽

Histone Synthesis ◽

A Minor ◽

Nucleolar Rna

In order to investigate some of the cytochemical processes involved in interphase growth and culminating in cell division, a combined autoradiographic and microphotometric study of nucleic acids and proteins was undertaken on statistically seriated cells of Vicia faba root meristems. Adenine-8-C14 and uridine-H3 were used as ribonucleic acid (RNA) precursors, thymidine-H3 as a deoxyribonucleic acid (DNA) precursor, and phenylalanine-3-C14 as a protein precursor. Stains used in microphotometry were Feulgen (DNA), azure B (RNA), pH 2.0 fast green (total protein), and pH 8.1 fast green (histone). The autoradiographic data (representing rate of incorporation per organelle) and the microphotometric data (representing changes in amounts of the various components) indicate that the mitotic cycle may be divided into several metabolic phases, three predominantly anabolic (net increase), and a fourth phase predominantly catabolic (net decrease). The anabolic periods are: 1. Telophase to post-telophase during which there are high rates of accumulation of cytoplasmic and nucleolar RNA and nucleolar and chromosomal total protein. 2. Post-telophase to preprophase characterized by histone synthesis and a diphasic synthesis of DNA with the peak of synthesis at mid-interphase and a minor peak just preceding prophase. The minor peak is coincident with a relatively localized DNA synthesis in several chromosomal regions. This period is also characterized by minimal accumulations of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA. 3. Preprophase to prophase in which there are again high rates of accumulation of cytoplasmic RNA, and nucleolar and chromosomal total protein and RNA. The catabolic phase is: 4. The mitotic division during which there are marked losses of cytoplasmic RNA and chromosomal and nucleolar total protein and RNA.

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Safranin O without fast green is the best staining method for testing the degradation of macromolecules in a cartilage extracellular matrix for the determination of the postmortem interval

Forensic Science Medicine and Pathology ◽

10.1007/s12024-019-00208-0 ◽

2019 ◽

Vol 16 (2) ◽

pp. 252-258 ◽

Cited By ~ 1

Author(s):

Armin Alibegović ◽

Rok Blagus ◽

Inigo Zubiavrre Martinez

Keyword(s):

Extracellular Matrix ◽

Staining Method ◽

Postmortem Interval ◽

Fast Green ◽

Green Is ◽

Cartilage Extracellular Matrix ◽

Safranin O

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Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

Foods ◽

10.3390/foods10122955 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2955

Author(s):

Miroslava Jandová ◽

Pavel Měřička ◽

Michaela Fišerová ◽

Aleš Landfeld ◽

Pavla Paterová ◽

...

Keyword(s):

Risk Assessment ◽

Human Milk ◽

Cold Storage ◽

Systematic Study ◽

Quantitative Data ◽

Bacterial Contamination ◽

Average Amount ◽

Milk Bank ◽

Human Milk Bank ◽

Frequent Reason

A systematic study, performed from 2017–2020 looked at the rate of positive post-pasteurization B. cereus findings, the quantity of B. cereus in pasteurized banked human milk (PBM), and the rate of B. cereus toxicogenic isolates from PBM. During the study period, 6815.71 L (30,943 tested bottles) of PBM were tested, with an average amount per year of 1703.93 L (7736 tested bottles). The PBM discard rate per year due to bacterial contamination varied between 8.7–10.0% and contamination with B. cereus was the most frequent reason. The total number of B. cereus positive tests was 2739 and the proportion of its positivity from all positive tests was between 56.7–66.6%. The prevalence of B. cereus positive tests rose significantly in the summer months. The production of enterotoxin was found in 3 of the 20 tested samples (15.0%). The B. cereus CFU-quantities in the PBM were below 10 CFU/mL in 80% of cases (16 of 20 samples tested). The quantitative data can be used in the risk assessment of cold storage of PBM at temperatures above zero and manipulation of PBM prior to its administration.

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Metallurgical Effects of Grain Boundary Ledges

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100078225 ◽

1977 ◽

Vol 35 ◽

pp. 126-129

Author(s):

L.E. Murr

Keyword(s):

Grain Boundary ◽

Quantitative Data ◽

Mechanical Treatment ◽

Unit Length ◽

Physical And Mechanical Properties ◽

Direct Observations ◽

Transmission Electron ◽

Properties Of Materials ◽

Thermo Mechanical Treatment ◽

Ledge Density

Ledges in grain boundaries can be identified by their characteristic contrast features (straight, black-white lines) distinct from those of lattice dislocations, for example1,2 [see Fig. 1(a) and (b)]. Simple contrast rules as pointed out by Murr and Venkatesh2, can be established so that ledges may be recognized with come confidence, and the number of ledges per unit length of grain boundary (referred to as the ledge density, m) measured by direct observations in the transmission electron microscope. Such measurements can then give rise to quantitative data which can be used to provide evidence for the influence of ledges on the physical and mechanical properties of materials.It has been shown that ledge density can be systematically altered in some metals by thermo-mechanical treatment3,4.

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Morphometric analysis of cellular interactions with the endothelium during the development of arterial lesions using Scanning Electron Microscopy and digital image analysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128870 ◽

1987 ◽

Vol 45 ◽

pp. 918-919

Author(s):

M.E. Rosenfeld ◽

C. Karboski ◽

M.F. Prescott ◽

P. Goodwin ◽

R. Ross

Keyword(s):

Electron Microscopy ◽

Image Analysis ◽

Quantitative Data ◽

Digital Image Analysis ◽

Lower Cost ◽

Matched Control ◽

Digital Analysis ◽

Cellular Interactions ◽

Arterial Lesions ◽

Scanning Electron

Previous research documenting the chronology of the cellular interactions that occur on or below the surface of the endothelium during the initiation and progression of arterial lesions, primarily consisted of descriptive studies. The recent development of lower cost image analysis hardware and software has facilitated the collection of high resolution quantitative data from microscopic images. In this report we present preliminary quantitative data on the sequence of cellular interactions that occur on the endothelium during the initiation of atherosclerosis or vasculitis utilizing digital analysis of images obtained directly from the scanning electron microscope. Segments of both atherosclerotic and normal arteries were obtained from either diet-induced or endogenously (WHHL) hypercholesterolemic rabbits following 1-4 months duration of hypercholesterolemia and age matched control rabbits. Vasculitis was induced in rats following placement of an endotoxin soaked thread adjacent to the adventitial surface of arteries.

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Motility of leukemic lymphocytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159382 ◽

1990 ◽

Vol 48 (3) ◽

pp. 368-369

Author(s):

Manoj Raje ◽

Karvita B. Ahluwalia

Keyword(s):

Quantitative Data ◽

Laser Doppler Velocimetry ◽

Acute Lymphocytic Leukemia ◽

Lymphocytic Leukemia ◽

Cellular Space ◽

Differentiated Cells ◽

Poorly Differentiated ◽

Solid Tissues ◽

Well Differentiated ◽

Reduced Mobility

In Acute Lymphocytic Leukemia motility of lymphocytes is associated with dissemination of malignancy and establishment of metastatic foci. Normal and leukemic lymphocytes in circulation reach solid tissues where due to in adequate perfusion some cells get trapped among tissue spaces. Although normal lymphocytes reenter into circulation leukemic lymphocytes are thought to remain entrapped owing to reduced mobility and form secondary metastasis. Cell surface, transmembrane interactions, cytoskeleton and level of cell differentiation are implicated in lymphocyte mobility. An attempt has been made to correlate ultrastructural information with quantitative data obtained by Laser Doppler Velocimetry (LDV). TEM of normal & leukemic lymphocytes revealed heterogeneity in cell populations ranging from well differentiated (Fig. 1) to poorly differentiated cells (Fig. 2). Unlike other cells, surface extensions in differentiated lymphocytes appear to originate by extrusion of large vesicles in to extra cellular space (Fig. 3). This results in persistent unevenness on lymphocyte surface which occurs due to a phenomenon different from that producing surface extensions in other cells.

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