Chiral FexCuySe nanoparticles as peroxidase mimics for colorimetric detection of 3, 4-dihydroxy-phenylalanine enantiomers

2021 ◽  
Author(s):  
Shuyang Hu ◽  
Qiuyan Shuai ◽  
Yulong Lin ◽  
Yan Fu ◽  
Meng Li
Keyword(s):  
Quality Control ◽  
Colorimetric Detection ◽  
Cost Effective ◽  
Drug Analysis ◽  
High Catalytic Activity ◽  
Chiral Ligand ◽  
Peroxidase Mimic ◽  
Disease Treatment ◽  
Peroxidase Mimics

Abstract L-3,4-dihydroxy-phenylalanine (L-dopa) is the most widely used drug in Parkinson's disease treatment. However, development of cost-effective and high-throughput sensors to accurate enantioselective discrimination of L-dopa and D-dopa remains challenging to date. Herein, on the basis of the peroxidase-mimic activity of chiral FexCuySe nanoparticles, we demonstrated a novel colorimetric sensor for determination of chiral dopa. The surface chiral ligand, L/D-histidine (L/D-His), endowed the nanozymes with enantioselectivity in catalyzing the oxidation of dopa enantiomers. According to the values of kcat/Km, the efficiency of L-His modified nanoparticle (L-FexCuySe NPs) towards L-dopa was 1.56 times higher than that of D-dopa. While, D-His can facilely reverse the preference of the nanozyme to D-dopa. On the basis of high catalytic activity and enantioselectivity of L-FexCuySe NPs in oxidation of L-dopa, the L-FexCuySe NPs based system can be utilized for detection of L-dopa. The linear ranges for L-dopa determination were 5 µM to 0.125 mM and 0.125 mM to 1 mM with a detection limit of 1.02 µM. Critically, the developed sensor has been successfully applied in the quality control of clinical used L-dopa tablets. Our work sheds light on developing simple and sensitive chiral nanomaterials-based sensors for drug analysis.

Colorimetric Detection of Hg(II) Sensor Based on Mos2 Nanosheets Acting as Peroxidase Mimics

2021 ◽  
Vol 37 (3) ◽  
pp. 679-682
Author(s):  
S. Kavitha ◽  
S. Mary Jelastin Kala ◽  
A. Anand Babu Christus
Keyword(s):  
Colorimetric Detection ◽  
High Sensitivity ◽  
Colorimetric Determination ◽  
X Ray Diffraction ◽  
Mos2 Nanosheets ◽  
Peroxidase Mimic ◽  
Sem Image ◽  
Peroxidase Mimics ◽  
Ft Ir

This paper presents colorimetric determination of Hg(II) based on MoS2 nanosheets with peroxidase mimics activity. The structure of the this sensor by the peroxidase mimic activity material of MoS2 nanosheets with TMB (Tetramethylbenzidine) solution, the colorimetric detection target of Hg(II) is determined by on-off mechanism using biomolecule of cysteine. The MoS2 nanosheets evaluated by X-ray diffraction, FT-IR and SEM image, confirms formation of a flower like structure. Our results shows that a simple colorimetric detection using peroxidase mimic mechanism can be used to MoS2 nanosheets and determine the Hg(II) in aqueous solution with high sensitivity (10 nM) comparable to those of other nanomaterials. The result suggests that MoS2 nanosheets is a promising new and simple colorimetric sensor for applications in environmental and biological applications.

scholarly journals Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

2014 ◽  
Vol 50 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Edith Cristina Laignier Cazedey ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Hydrochloric Acid ◽  
Spectrophotometric Method ◽  
Cost Effective ◽  
Pharmaceutical Formulation ◽  
Dissolution Studies ◽  
Linearity Range ◽  
Development And Validation ◽  
Routine Quality Control

New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.

scholarly journals Application of drug–metal ion interaction principle in conductometric determination of imatinib, sorafenib, gefitinib and bosutinib

2020 ◽  
Vol 18 (1) ◽  
pp. 798-807
Author(s):  
Hassan A. Alhazmi ◽  
AbdulRhman Ali Bokar Nasib ◽  
Yasser Ali Musleh ◽  
Khaled Qassim Hijri ◽  
Zia ur Rehman ◽  
...  
Keyword(s):  
Quality Control ◽  
Anticancer Agents ◽  
Metal Ion ◽  
Cost Effective ◽  
Measured Concentration ◽  
Maximum Reduction ◽  
Analyte Solution ◽  
Effective Analysis ◽  
Drug Solution

AbstractAn analytical method for the quantification of anticancer agents such as imatinib, sorafenib, gefitinib and bosutinib using conductometry was developed. Each drug solution was mixed with measured concentration of metal ion (Cu2+) solution resulting in drug–metal ion complexation in the titration cell. Conductance was progressively decreased on addition of the analyte solution up to a point of maximum reduction, that is, the end point. Corrected conductance values were calculated from the observed conductance and used to plot a graph against the volume of drug solution added. No interferences were observed from blank and placebo as they gave no clear inflection in the conductivity during titration. The precision and the accuracy of the developed method was established by the analysis of quality control samples; %RSD of corrected conductance values <2% and recovery results within 100 ± 2% were achieved. The calibration graphs obtained were linear over the concentrations 1.0–1.4 mM for all the drugs (R2 > 0.99). The drugs were successfully analyzed in their respective dosage forms prepared in-house. The method has offered easier, faster and cost-effective analysis of the selected drugs and can be used for routine determinations in the quality control laboratories. More importantly, it is an environmental friendly procedure, as no organic solvent was used throughout the analysis.

PSS-GN nanocomposites as highly-efficient peroxidase mimics and their applications in colorimetric detection of glucose in serum

RSC Advances ◽  
2015 ◽  
Vol 5 (110) ◽  
pp. 90400-90407 ◽  
Author(s):  
Jing Chen ◽  
Jia Ge ◽  
Lin Zhang ◽  
Zhaohui Li ◽  
Saisai Zhou ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Serum Samples ◽  
Highly Efficient ◽  
Peroxidase Mimics

PSS-GN nanocomposites were firstly proposed as a peroxidase-like mimic successfully and utilized in the determination of glucose in serum samples.

scholarly journals Quantitative Conductometric Determination of Sitagliptin, Linagliptin, Vildagliptin and Alogliptin by Applying the Concept of Drug-Metal Ion Interaction

2019 ◽  
Vol 35 (5) ◽  
pp. 1597-1604
Author(s):  
Mohammed Al- Bratty ◽  
Hatim Murayzin ◽  
Adel Almanaa ◽  
Mohammed Qasem Tawhari ◽  
Zia Ur Rehman ◽  
...  
Keyword(s):  
Metal Ion ◽  
Current Method ◽  
Cost Effective ◽  
Drug Analysis ◽  
Dosage Forms ◽  
Fast Analysis ◽  
Analytical Technique ◽  
Economical Analysis ◽  
Analyte Solution

Cost of analysis and length of analytical procedure are among the most concerning factors in drug analysis. As conductometric analysis has been considered to be relatively inexpensive analytical technique offering fast analysis of drugs, in this study our aim was to develop a rapid and cost-effective method for quantitative determination of sitagliptin, linagliptin, vildagliptin and alogliptin in bulk and dosage forms. The test drugs were allowed to complex with metal ion (Cu2+) in the titration cell, which resulted in the change of conductance of the solution. The corrected conductance was calculated and graph was plotted between corrected conductance and the volume of the analyte solution added. The point of maximum change in the corrected conductance was considered as end point of the titration. The method was found to be linear in the concentration range of 1.0 – 1.4 mM for all analytes with good correlation coefficient (R2 ˃ 0.999). The %RSD of the corrected conductance values were in the range of 0.046-1.837, while the recovery of analytes were within 100 ± 2%, indicating that the method was precise and accurate. The specificity of the method was demonstrated by no interference from blank and placebo. The method was successfully applied for quantitative analysis of all the drugs in the dosage forms. The current method has a major advantage that it provided easy, fast and economical analysis of sitagliptin, linagliptin, vildagliptin and alogliptin in bulk drugs and formulations using conductivity meter. KEYWORDS:

Method for Trifuzol-Neo assay determination by GC-MS

2021 ◽  
pp. 4523-4528
Author(s):  
A.A. Yaroshenko ◽  
V.V. Parchenko ◽  
O.A. Bihdan ◽  
O.I. Panasenko ◽  
Yu V. Karpenko ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Quality Control ◽  
Mobile Phase ◽  
Raw Materials ◽  
Cost Effective ◽  
Scientific Interest ◽  
Research Information ◽  
Agricultural Chemistry ◽  
Derivatives Of

The derivatives of 1,2,4-triazole are of the great scientific interest in the fields of pharmaceutics, agricultural chemistry, and construction The production of Trifuzol-Neo has already been launched, and it is now commercially available. Compounds based on 1,2,4-triazole may also be useful in veterinary practice due to their antiviral activity. Trifuzol-Neo, or piperidinium ([5-(2-furanyl)-4-phenyl-4Н-1,2,4-triazol-3-yl]thio)acetate (Figure 1), has already proved to be an effective immunostimulator for poultry.Since that, the quality control of raw materials and the ready product is required during the production stage. Currently, there is only one method for determination of the assay of Trifuzol-Neo, which involves HPLC-DMD (Center of Medical Research Information and Patent and Licensing Practice of Ukraine. The main disadvantages of HPLC-DMD are the use of relatively high amounts of costly solvents (acetonitrile in this case), considerable baseline instability, and time-consuming system stabilization. Gas-chromatography is able to avoid these constrictions since it involves gas as a mobile phase, which is a more cost-effective and reproducible alternative. The newly developed GC/MS method for Trifuzol-Neo assay determination consists of the following steps: weighing 20.0mg of Trifuzol-Neo powder, dillution in 20mL methanol, injection of 0.5uL of the obtained solution to GC column (at least three times). The same operations are done with a Trifuzol-Neo standard.

scholarly journals Fe3O4Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H2O2

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 210 ◽  
Author(s):  
Qingtong Zhang ◽  
Mingfu Li ◽  
Chenyan Guo ◽  
Zhuan Jia ◽  
Guangcong Wan ◽  
...  
Keyword(s):  
Self Assembly ◽  
Limit Of Detection ◽  
Paper Industry ◽  
Colorimetric Detection ◽  
Renewable Resource ◽  
High Catalytic Activity ◽  
Blue Color ◽  
Optimal Conditions ◽  
Peroxidase Mimic ◽  
Assembly Method

Lignin is the second largest naturally renewable resource and is primarily a by-product of the pulp and paper industry; however, its inefficient use presents a challenge. In this work, Fe3O4 nanoparticles loaded on lignin nanoparticles (Fe3O4@LNPs) were prepared by the self-assembly method and it possessed an enhanced peroxidase-like activity. Fe3O4@LNPs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to generate a blue color, was observable by the naked eye. Under the optimal conditions, Fe3O4@LNPs showed the ability of sensitive colorimetric detection of H2O2within a range of 5–100 μM and the limit of detection was 2 μM. The high catalytic activity of Fe3O4@LNPs allows its prospective use in a wide variety of applications, including clinical diagnosis, food safety, and environmental monitoring.

UV spectroscopic method for determination of phenytoin in bulk and injection forms

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Spectroscopic Method ◽  
Ich Guidelines ◽  
Precision And Accuracy ◽  
Routine Quality Control

Recent study was conducted to develop a simple UV spectrophotometric method to determine Phenytoin in bulk and injection form according to official requirement and validate as per ICH guidelines. λmax of Phenytoin was found 202 nm. Linearity existed perceived in the concentration assortment 2-8 μg/ml (r2 = 0.999) for the method. The method was validated pertaining to linearity, precision and accuracy studies, LOD and LOQ consistent with ICH guidelines. The existent method was establish to be simple, linear, precise, accurate as well as sensitive and can be applied for routine quality control enquiry for the analysis of Phenytoin in bulk and injection form.

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