scholarly journals Phytochemical screening and spectrum profile of functional group from Banana (Musa paradisiaca L.) stem waste extract using Fourier-transform Infrared (FTIR) Spectroscopy method

2021 ◽  
Vol 1882 (1) ◽  
pp. 012106
Author(s):  
R C Purnama ◽  
A Primadiamanti
2021 ◽  
pp. 002580242110109
Author(s):  
Sweety Sharma ◽  
Rito Chophi ◽  
Jaskirandeep Kaur Jossan ◽  
Rajinder Singh

The most important task in a criminal investigation is to detect and identify the recovered biological stains beyond reasonable scientific doubt and preserve the sample for further DNA analysis. In the light of this fact, many presumptive and confirmatory tests are routinely employed in the forensic laboratories to determine the type of body fluid. However, the currently used techniques are specific to one type of body fluid and hence it cannot be utilized to differentiate multiple body fluids. Moreover, these tests consume the samples in due process, and thus it becomes a great limitation especially considering the fact that samples are recovered in minute quantity in forensic cases. Therefore, such limitations necessitate the use of non-destructive techniques that can be applied simultaneously to all types of bodily fluids and allow sample preservation for further analysis. In the current work, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy has been used to circumvent the aforementioned limitations. The important factors which could influence the detection of blood such as the effect of substrates, washing/chemical treatment, ageing, and dilution limits on the analysis of blood have been analysed. In addition, blood discrimination from non-blood substance (biological and non-biological in nature) has also been studied. Chemometric technique that is PCA–LDA has been used to discriminate blood from other body fluids and it resulted in 100% accurate classification. Furthermore, blood and non-blood substances including fake blood have also been classified into separate clusters with a 100% accuracy, sensitivity, and specificity. All-inclusive, this preliminary study substantiates the potential application of ATR-FTIR spectroscopy for the non-destructive identification of blood traces in simulated forensic casework conditions with 0% rate of false classification.


2016 ◽  
Vol 19 (12) ◽  
pp. 2718-2725 ◽  
Author(s):  
Yuny Erwanto ◽  
Afif Turindra Muttaqien ◽  
Sugiyono ◽  
Sismindari ◽  
Abdul Rohman

2021 ◽  
Vol 4 (2) ◽  
pp. 66-72
Author(s):  
Robby Candra Purnama ◽  
Annisa Primadiamanti

Kepok banana plants contain secondary metabolites such as tannins and flavonoids. Tannins and flavonoids have various properties for human health. Research has been carried out to identify secondary metabolite compounds (tannins, flavonoids, and saponins) by using the phytochemical screening method to see the functional group profile contained in the extract of kepok banana stem waste. Kepok banana stem waste was extracted in 96% ethanol, then evaporated and screened phytochemically. This extract was used to prepare effervescently. Screening results showed that tannin and flavonoids were identified by the appearance of the following color black-green and dark red, respectively. Meanwhile, saponins were negative because the foam formed had a height of 0.3 cm and did not meet the saponins' positive requirements (1-3 cm high foam and stable for 5 minutes). Identification of functional groups in the extract of kepok banana stem waste using Fourier-transform Infrared Spectroscopy (FTIR) showed that C-C stretching in the area 2927.24 cm-1, O-H stretching in the 3423.87 cm-1 area, C=O stretching in the 1648.87 cm-1 area. Also appeared bending CH2 in the region of 1421.45 cm-1, and C-C in the area of 1149.98 cm-1. The characteristics of three different formulas (A, B, and C) of effervescent have been investigated: the moisture content of 2.51%; 2.55%, and 2.52%, respectively. Then, flow rate of 8.81 g/s; 8.83 g/s; and 8.82 g/s, compressibility of 14.5%; 14.4%; and 14.5%, and a pH of 5.97; 5.98; and 5.97 respectively. All parameters are eligible.


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