Single and mixture administration of white tea (Camellia sinensis) and moringa (Moringa oleifera) as antihyperglicemia in diabetic male rats (Sprague-dawley) induced by streptozotocin

AbstractBackgroundMoringa oleifera seed has anti-diabetic and anti-obesogenic properties. This study interrogated the effect of crude hydroethanolic M. oleifera seed extract on the blood markers of metabolic syndrome (MetS) in high-fructose diet fed growing Sprague-Dawley rats.MethodsSixty 21-day old female and male Sprague-Dawley rat pups were randomly allocated to and administered one of the following treatment regimens daily for twelve weeks: group I – plain drinking water (PW)+plain gelatine cube (PC), group II – 20% (w/v) fructose solution (FS)+PC, group III – FS+100 mg/kg body mass fenofibrate in gelatine cube (FN), group IV – FS+low dose (50 mg/kg body mass) of M. oleifera in gelatine cube (LMol) and group V – FS+high dose (500 mg/kg body mass) of M. oleifera in gelatine cube (HMol). The rats in each treatment regimen had ad libitum access to a standard rat chow. After the 12-week trial, the rats were subjected to an oral glucose tolerance test and then euthanised 48 h later. Blood was collected. Plasma triglyceride, cholesterol and insulin concentration were determined. HOMA-IR was then computed.ResultsThe high-fructose diet increased (p<0.05) plasma insulin concentration and HOMA-IR in female rats only. It increased plasma triglyceride concentration in both female and male rats and plasma cholesterol concentration in male rats only. The crude hydroethanolic M. oleifera seed extract prevented the high-fructose diet-induced metabolic derangements in male and female rats.ConclusionCrude hydroethanolic M. oleifera seed extract can potentially be used as a prophylactic intervention for diet-induced MetS in children.

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Pengaruh pemberian nano daun kelor (moringa oleifera) terhadap kadar mineral serum dan tulang pada tikus sprague dawley jantan tumbuh

JURNAL GIZI INDONESIA ◽

10.14710/jgi.7.2.114-120 ◽

2019 ◽

Vol 7 (2) ◽

pp. 114-120

Author(s):

Syahrial Syahrial ◽

Rimbawan Rimbawan ◽

Evy Damayanthi ◽

Dewi Apri Astuti ◽

Pipih Suptijah

Keyword(s):

Group Intervention ◽

Moringa Oleifera ◽

Intervention Group ◽

Bioactive Compound ◽

Magnesium Level ◽

Male Rats ◽

Control Group ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Mineral Levels

Background: One of the important minerals for growth is calcium to build new bone. Moringa oleifera has been known as high nutritional content and bioactive compound tree that could act as antioxidant and improved several diseases like diabetes, arthritis, cardiovascular. Phytochemical analyses have shown that Moringa oleifera contain of high macro minerals like calcium, potasium, magnesium, and phosphor. Several previous studies have shown that mineral nanoparticle have higher bioavailability than natural compounds.. Objectives: The purpose of this study was to analyze the effect of moringa oleifera nanoparticle leaf on mineral serum and bone at growth stage of Sprague Dawley male rats. Methods: In this study, the pre and post controlled group design and complete random design was used. The subjects were 27 growing male Sprague dawley rats aged 2 month were devided into three group which received standard feed contain CaCO3 (control group), intervention feed contained 450nm and 750nm nano Moringa oleifera for 60 days. The data of the experiment was analyzed by paired T-test and Duncan test at 95% confidence level.Results: The result showed that there was no significance different for both of intervention group for mineral serum and fermur bone. While for the tibia bone, the result showed that the calcium, phosphor and magnesium were significantly increase (p<0.05). The intervention group received 450nm showed more effective improvement of magnesium level (p<0.05) than intervention group received 750nm.Conclusion: The intervention of moringa oleifera nanoparticle leaf had a positive effect in decreasing serum and bone mineral levels in male rats. There were significant increased at magnesium level measured in tibia bones after intervention, with higher increasing at group received 450nm nanoparticle.

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The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽

10.3390/ani11061569 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1569

Author(s):

Tomislav Šušnjar ◽

Ivana Kuzmić Prusac ◽

Ivan Švagelj ◽

Anđela Jurišić ◽

Tomislav Šušnjar ◽

...

Keyword(s):

Sperm Count ◽

Semen Analysis ◽

Study Groups ◽

Testicular Biopsy ◽

Male Rats ◽

Lower Percentage ◽

Control Group ◽

Sprague Dawley ◽

Left Testis ◽

Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

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Stamina-Enhancing Effects of Human Adipose-Derived Stem Cells

Cell Transplantation ◽

10.1177/09636897211035409 ◽

2021 ◽

Vol 30 ◽

pp. 096368972110354

Author(s):

Eun-Jung Yoon ◽

Hye Rim Seong ◽

Jangbeen Kyung ◽

Dajeong Kim ◽

Sangryong Park ◽

...

Keyword(s):

Physical Activity ◽

Stem Cells ◽

Locomotor Activity ◽

Tissue Injury ◽

Male Rats ◽

Adipose Derived Stem Cells ◽

Sprague Dawley ◽

Serum Triglycerides

Stamina-enhancing effects of human adipose derived stem cells (hADSCs) were investigated in young Sprague-Dawley rats. Ten-day-old male rats were transplanted intravenously (IV) or intracerebroventricularly (ICV) with hADSCs (1 × 106 cells/rat), and physical activity was measured by locomotor activity and rota-rod performance at post-natal day (PND) 14, 20, 30, and 40, as well as a forced swimming test at PND 41. hADSCs injection increased the moving time in locomotor activity, the latency in rota-rod performance, and the maximum swimming time. For the improvement of physical activity, ICV transplantation was superior to IV injection. In biochemical analyses, ICV transplantation of hADSCs markedly reduced serum creatine phosphokinase, lactate dehydrogenase, alanine transaminase, and muscular lipid peroxidation, the markers for muscular and hepatic injuries, despite the reduction in muscular glycogen and serum triglycerides as energy sources. Notably, hADSCs secreted brain-derived neurotrophic factor (BDNF) and nerve growth factor in vitro, and increased the level of BDNF in the brain and muscles in vivo. The results indicate that hADSCs enhance physical activity including stamina not only by attenuating tissue injury, but also by strengthening the muscles via production of BDNF.

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Effect of black tea brew of Camellia sinensis on sexual competence of male rats

Journal of Ethnopharmacology ◽

10.1016/j.jep.2008.04.023 ◽

2008 ◽

Vol 118 (3) ◽

pp. 373-377 ◽

Cited By ~ 20

Author(s):

W.D. Ratnasooriya ◽

T.S.P. Fernando

Keyword(s):

Camellia Sinensis ◽

Black Tea ◽

Male Rats

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Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v19i2.45011 ◽

2020 ◽

Vol 19 (2) ◽

pp. 296-303

Author(s):

Eni Widayati ◽

Taufiqurrachman Nasihun ◽

Azizah Hikma Savitri ◽

Nurina Tyagita

Keyword(s):

Protein Expression ◽

Caspase 3 ◽

Medical Science ◽

Liver Cells ◽

Male Rats ◽

Control Group ◽

Sprague Dawley ◽

Control Group Design ◽

Uvb Irradiation ◽

The Difference

Objective: The effect of Pimpinela alpina Molk (PaM) on decrease in Bax and Caspase-3 protein expression in liver cells apoptosis have been proven. However, the difference result between 7 and 15 days treatment duration of PaM need to be confirmed. This study aimed to confirm that treatment of PaM during 15 days is more effective decreasing Bax and Caspase-3 protein expression in liver cells following UVB irradiation. Methods: In the post test only control group design, 35 Sprague Dawley male rats, 300 gram body weight were divided into two arms, consisting of three groups respectively. First arm comprise Neg-7, PaM7-100, and PaM7-150. Second arm comprise Neg-15, PaM15-100, and PaM15-150. Nor-G was added as normal control neither exposed to UVB nor PaM treatment. In negative group was only radiated to UVB and PaM groups were exposed to UVB and treatment with 100, and 150 mg PaM per oral for 7 and 15 days respectively. At day 8 (first arm) and 16 (second arm), liver organ was taken and Bax and Caspase-3 protein expression assessed by Immunohistochemical staining method. Result: Post Hoc LSD analysis indicated that Bax and Caspase-3 protein expression in PaM15-100 and PaM15-150 was significant lower compared to that of Nor-G, PaM7-100, and PaM7-150, p < 0.05. Conclusion: Ttreatment of PaM with doses 100 and 150 mg for 15 days was better in decreasing Bax and Caspase-3 protein expression of liver cells following UVB irradiation. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.296-303

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Mesotheliomas and Proliferative Lesions of the Testicular Mesothelium Produced in Fischer, Sprague-Dawley and Buffalo Rats by Methyl(acetoxymethyl)nitrosamine (DMN-OAc)

Veterinary Pathology ◽

10.1177/030098587901600510 ◽

1979 ◽

Vol 16 (5) ◽

pp. 574-582 ◽

Cited By ~ 20

Author(s):

J. J. Berman ◽

J. M. Rice

Keyword(s):

Body Weight ◽

Tumor Cells ◽

Mesothelial Cells ◽

Male Rats ◽

Sprague Dawley ◽

Colloidal Iron ◽

Microscopic Appearance ◽

Intraperitoneal Dose ◽

Single Focus

A single intraperitoneal dose of methyl(acetoxymethyl)nitrosamine (13 mg/kg body weight) given to 78 5-week-old male rats induced 25 mesotheliomas; two mesotheliomas were found in 67 control rats. All mesotheliomas arose from the peritesticular mesothelium and had a typical microscopic appearance of branching papillary fronds with a collagenous core covered by one or many layers of plump tumor cells. Cytoplasm of tumor cells contained material that reacted positively to a colloidal iron stain and was labile to hyaluronidase. In addition to frank mesotheliomas, 16 lesions, which we called atypical mesothelial proliferations. were found. These consisted of a single focus of plump mesothelial cells overlying an area of thick stroma. Often these foci included short, non-branched papillary projections above the surface of adjacent normal mesothelium. Twelve of the 16 lesions occurred in methyl(acetoxymethyl)nitrosamine-treated rats.

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