scholarly journals Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

2020 ◽  
Vol 19 (2) ◽  
pp. 296-303
Author(s):  
Eni Widayati ◽  
Taufiqurrachman Nasihun ◽  
Azizah Hikma Savitri ◽  
Nurina Tyagita
Keyword(s):  
Protein Expression ◽  
Caspase 3 ◽  
Medical Science ◽  
Liver Cells ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Uvb Irradiation ◽  
The Difference

Objective: The effect of Pimpinela alpina Molk (PaM) on decrease in Bax and Caspase-3 protein expression in liver cells apoptosis have been proven. However, the difference result between 7 and 15 days treatment duration of PaM need to be confirmed. This study aimed to confirm that treatment of PaM during 15 days is more effective decreasing Bax and Caspase-3 protein expression in liver cells following UVB irradiation. Methods: In the post test only control group design, 35 Sprague Dawley male rats, 300 gram body weight were divided into two arms, consisting of three groups respectively. First arm comprise Neg-7, PaM7-100, and PaM7-150. Second arm comprise Neg-15, PaM15-100, and PaM15-150. Nor-G was added as normal control neither exposed to UVB nor PaM treatment. In negative group was only radiated to UVB and PaM groups were exposed to UVB and treatment with 100, and 150 mg PaM per oral for 7 and 15 days respectively. At day 8 (first arm) and 16 (second arm), liver organ was taken and Bax and Caspase-3 protein expression assessed by Immunohistochemical staining method. Result: Post Hoc LSD analysis indicated that Bax and Caspase-3 protein expression in PaM15-100 and PaM15-150 was significant lower compared to that of Nor-G, PaM7-100, and PaM7-150, p < 0.05. Conclusion: Ttreatment of PaM with doses 100 and 150 mg for 15 days was better in decreasing Bax and Caspase-3 protein expression of liver cells following UVB irradiation. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.296-303

scholarly journals Situation Awareness of Cough etiquettes of Hospital Security personnel and Janitors

2019 ◽  
Vol 18 (4) ◽  
pp. 814-817
Author(s):  
Kusbaryanto ◽  
Ekorini Listiowati
Keyword(s):  
Situation Awareness ◽  
Medical Science ◽  
Sampling Technique ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Healthcare Facilities ◽  
Security Personnel ◽  
Post Test ◽  
The Difference

Background: Nosocomial infection is a major problem in hospitals around the world. To minimize the risk of infection in hospitals and other healthcare facilities, cough etiquette has to be applied. Thus, good situation awareness and the understanding why cough etiquette is important are necessary. The purpose of this study was to find out the effective education on cough etiquette on hospital security personnel’s and janitors’ situation awareness on cough etiquette. Methodology: This research study followed quasy experimental design with pretest and post test control group design. The sampling technique was purposive sampling involving 24 respondents for experimental group and 27 respondents for the control group. The analysis of the data employed Wilcoxon test and it employed Mann Whitney test to find out the difference of independent variable medians. Questionnaires were used to gather data collection. Results: The result of the analysis presented a mean of 97,7 on the post-test of control group and a mean of 116,3 on the post-test of treatment group. Wilcoxon and Mann Whitney tests resulted p = 0,001 (< 0,05), which meant there were significant differences. Conclusion: Education on cough etiquette is effective to raise hospital security personnel and the janitors’ awareness on cough etiquette. Bangladesh Journal of Medical Science Vol.18(4) 2019 p.814-817

Protective effect of salusin-α and salusin-β against ethanol-induced gastric ulcer in rats

2017 ◽  
Vol 28 (6) ◽  
Author(s):  
Ayhan Tanyeli ◽  
Ersen Eraslan ◽  
Elif Polat ◽  
Tuğba Bal
Keyword(s):  
Caspase 3 ◽  
Mucosal Injury ◽  
Heart Tissue ◽  
Male Rats ◽  
Gastric Ulcers ◽  
Gastric Injury ◽  
Control Group ◽  
Sprague Dawley ◽  
Gastric Mucosal Lesions ◽  
Α Level

AbstractBackground:Alcohol consumption has been found to be associated with gastric ulcers, including gastric mucosal lesions. Salusin-α and salusin-β are bioactive peptides having 28 and 20 amino acids, respectively. Salusin-α and salusin-β immunoreactivity has been detected in the stomach and in the intestines. It has been reported that the salusins regulate the cytokine levels and decrease the infarct area in the heart tissue after ischemia. In this study, we investigated the effects of the salusins in the gastric injury formed with ethanol.Methods:Thirty-two sprague Dawley male rats were randomly divided into four groups, including eight rats in each group as follows: Group 1: control; Group 2: ethanol 5 mL/kg; Group 3: ethanol 5 mL/kg+5 nmol/kg salusin-α; Group 4: ethanol 5 mL/kg+5 nmol/kg salusin-β.Results:The salusin-α level increased at a significant level in the ulcer group formed with ethanol (p<0.001); the change in the salusin-β level is not significant. As for malondialdehyde (p<0.05) and myeloperoxidase (p<0.001), when compared with the control group, tumor necrosis factor-α (p<0.05) levels increased in the group to which ethanol was applied and decreased significantly with the application of salusins. Levels of GSH and IL-1β did not change at a significant level. In addition, histopathologic analysis demonstrated that, in salusin-administered groups, mucosal injury and caspase-3 expressions were reduced.Conclusions:The suppression of salusin-α and salusin-β on caspase-3 expression by means of their effects on oxidative injury and TNF-α levels shows that these two hormones could serve as anti-ulcerative agents.

scholarly journals Pengaruh yoghurt dan soyghurt kayu manis (Cinnamomum burmannii) terhadap kadar glukosa darah, insulin serum, dan malondialdehyde tikus pra sindrom metabolik

2020 ◽  
Vol 8 (1) ◽  
pp. 60
Author(s):  
Ninik Rustanti ◽  
Vifin Zakiahtin Nafsih ◽  
Rosita Nur Avisha ◽  
Dewi Marfu’ah Kurniawati ◽  
Rachma Purwanti ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Cell Damage ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
The Difference

Background: Pre metabolic syndrome is characterized by two of five risk factors: central obesity, dyslipidemia, hypertension, and increased fasting blood glucose. Cinnamon yogurt and soygurt contain antioxidants and fiber which can improve insulin sensitivity and blood glucose homeostasis and prevent cell damage in pre-metabolic syndrome conditionsObjective: This study aimed to determine the effect of cinnamon yogurt and soygurt on fasting blood glucose (FBG), serum insulin, and malondialdehyde (MDA) levels in pre-metabolic syndrome rats.Method: This study was an experimental study with a pre and post-test control group design. The subjects were 15 male Sprague Dawley rats which were divided into 5 normal control mice (K) and 10 pre metabolic syndrome mice with a diet high in fat and fructose for group P1 (yogurt) and P2 (soygurt) each of 5 mice. The yogurt and soygurt were given as much as 3.4 ml / g BW for 28 days. FBG levels were measured by the GOD-PAP method, while serum insulin and MDA levels were by the ELISA method. Different tests before and after treatment using Paired t-test or Wilcoxon. The difference tests between groups using the One-Way ANOVA test or Kruskal Wallis.Results: There were no differences in FBG and MDA levels between groups after intervention (p> 0.05). The highest percentage reduction in FBG in the P2 (-11.59%), then P1 (-4.06%). The decrease in MDA levels in group P1 = 19.17%, and P2 = 15.44% lower than K = 24.43%. After the intervention, the insulin level in group P2 (0.46 ng / ml) was significantly higher than P1 (0.318 ng/ml), but both were not different from K (0.384 ng / ml).Conclusion: There was no significant effect on the administration of cinnamon yogurt and soygurt to FBG, serum insulin, and MDA levels.

scholarly journals RUSIP SEHUBUNGAN PROFIL LIPID DALAM KEADAAN HIPERKOLESTEROLEMIA

2018 ◽  
Vol 20 (2) ◽  
pp. 97
Author(s):  
Indranila KS ◽  
Satrianugraha MD
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
White Male ◽  
Hdl Cholesterol ◽  
Male Rats ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Sprague Dawley ◽  
Research Subjects ◽  
The Difference

Rusip is a fermented fish product made from anchovies. Several lactic acid bacteria have been identified in rusip fermentation. Lacticacid bacteria are known to be able to reduce serum cholesterol. The aim of the research was to know the lipid profile changes due toadministration of rusip in hypercholesterolemia rats. The research was done by using Pre and Post Randomized Controlled Group Design.The research subjects consisted of twenty-eight Sprague Dawley strain white male rats aged 20 weeks with normal body weight, who weregiven a high-fat high-cholesterol diet, then divided into four (4) groups: control group, without Rusip administration; P1, P2 and P3 whoreceived 2.55; 5.10 and 7.65 mg Rusip/g body weight. After 14 days treatment, blood samples were taken to determine the lipid profileusing enzymatic methods. ANOVA or alternative test, was used to test the difference between the groups at the 95% confidence level. Basedon the research, the administration of Rusip in the treatment group caused significant changes in the lipid profiles of hypercholesterolemiarats (p<0.05). The total cholesterol, triglycerides and LDL cholesterol levels decreased significantly in all three treatment doses (p<0.05).The HDL cholesterol increased significantly in all three treatment doses (p<0.05). The highest change in lipid profile results was obtainedin the treatment of P3 to all variables. In this study, it was found that administration of Rusip could improve the state of blood lipidprofiles of hypercholesterolemia rats. Whereas, the highest changes were obtained in treatment with P3.

scholarly journals Comparison between Effectiveness of Pomegranate Juice (Punica granatum) and Simvastatin for Lowering Blood LDL Level in Hypercholesterolemic Male Rats (Rattus novergicus)

2016 ◽  
Vol 15 (2) ◽  
pp. 216-219
Author(s):  
Ariani Ariani ◽  
Putrya Hawa ◽  
Syaefudin Ali Akhmad
Keyword(s):  
Medical Science ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Beneficial Effects ◽  
Simvastatin Group ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Coronary heart disease (CHD) is one of the leading causes of death in developed and developing countries. In Indonesia 26% of death is caused by CHD. Hypercholesterolemia is one of the risk factors for CHD. Simvastatin is a hypolipidemic drug which has a significant number of side effects such as myopathy. While, Pomegranate juice (Punica granatum) contains anthocyanin, cathechins, tanin, vitamin C and vitamine E which have beneficial effects to decrease blood LDL level. Objective: The aim of this study was to compare the effectiveness of pomegranate juice (Punica granatum) and simvastatin in lowering blood LDL level of hypercholesterolemic male rats (Rattus novergicus).Methodology: This experimental study was executed following by pre and post control group design. This study used 15 male Wistar rats that were divided into three groups as 2% DMSO, simvastatin and pomegranate juice. Blood LDL level was examined at day-0 and day-15 of trial period. Result: Dependent T-test reveals that both pomegranate juice and simvastatin group showed significant difference in lowering blood LDL level before and after treatment. The result of Kruskal Wallis test showed that both pomegranate juice and simvastatin group have more significant efficacy than 2% DMSO. On the other hand, there was no significant difference between effectiveness of pomegranate juice and simvastatin group. Conclusion: Both Pomegranate juice and simvastatin are effective in lowering blood LDL level of hypercholesterolemia male rats and there is no significant difference between effectiveness of pomegranate juice and simvastatin.Bangladesh Journal of Medical Science Vol.15(2) 2016 p.216-219

scholarly journals Efek anthiperkolesterol snack bars beras ketan hitam (Oryza sativa var. glutinosa) labu kuning (Cucurbita moschata) pada tikus Sprague dawley

2021 ◽  
Vol 26 (1) ◽  
pp. 37
Author(s):  
Akhmad Mustofa ◽  
Nanik Suhartatik ◽  
Ratna Dewi Nugraheni
Keyword(s):  
Total Cholesterol ◽  
White Male ◽  
Male Rats ◽  
Blood Cholesterol ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
White Rats ◽  
Glutinous Rice ◽  
Pumpkin Powder

Snack bars from black glutinous rice and yellow pumpkin powder has been developed. The aim of the research was to evaluate the effect of the snack bars to the lipid profile of white male rats Sprague dawley. The experimental design of the research was Pre-Post Test Randomized Control Group Design using 25 male white rats. The rats were divided randomly into 5 groups, which was Group 1 as negative control (standard feed), Group 2 as positive control (feed with lard for hyper cholesterolemia condition, Group 3 was treated with simvastatin (0.18 mg / 200gBB), Group 4 treated with snack bars A (450mg / kgBB) and Group 5 was treated with snack bars B (450mg / kg BB). The differences between snack bar A and B were the proportion of black glutinous rice and yellow pumpkin powder. The snack bar was given orally. Total cholesterol, HDL, LDL, and triglycerides after 14 days of the treatment. According to the statistical analysis (p<0.05), the results showed that group with simvastatin has no significant differences with group with snack bars A.  Group with simvastatin, snack bars A, and snack bars B significantly influence blood cholesterol levels, total cholesterol, triglyceride levels, and LDL levels.  Mainly, snack bars A could decline blood cholesterol as good as simvastatin. Snack bars A have a higher portion of black glutinous rice than snack bars B.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Abstract P211: Single High Na + Ingestion Leads To An Increase In Oxidative Stress And Triggers Inflammation.

Hypertension ◽  
2021 ◽  
Vol 78 (Suppl_1) ◽  
Author(s):  
Ginette Bordcoch ◽  
Ivan Tavera Busso ◽  
Juan Masjoan Juncos ◽  
Luis I Juncos
Keyword(s):  
Oxidative Stress ◽  
The United States ◽  
Male Rats ◽  
Control Group ◽  
Aortic Tissue ◽  
Tissue Samples ◽  
Extracellular Signal ◽  
High Prevalence ◽  
Markers Of Oxidative Stress ◽  
The Difference

Hypertension has been linked to a progressive increased in oxidative stress and inflammation. The high prevalence of hypertension poses a great risk to public health as 108 million adults in the United States have the condition. For that reason, a better understanding of the link between a high Na+ intake and the development of hypertension is of crucial importance. We hypothesize that a single ingestion of a high Na+ solution leads to increased oxidative stress and triggers an inflammatory response. Wistar 200-250 g male rats had gastric infusions through the esophagus. Groups were infused with 8 mL liquid Vaseline (Control), 8 mL of NaCl 0.684 M (4% m/v), and 8 mL of NaCl 1.368 M (8% m/v). After infusion, blood was collected at different time points during the first hour. Tissue samples were obtained from the aorta, heart, and kidney. Electron Microscopy (EM) was performed on all tissues, which were also analyzed for molecular markers of oxidative stress: Superoxide Dismutase (SOD) and Malondialdehyde (MDA), and an inflammation marker: Extracellular Signal-Regulated Kinase (ERK). At 2 and a half minutes, serum Na+ concentration was unchanged in the control group compared to an increase observed in animals receiving 4% and 8% Na+ with concentrations of 135±1.4 mEq/L, 141±2.0 mEq/L, and 140±1.2 mEq/L respectively. At the 1-hour time point after infusion, the difference was further increased in the 8% group with serum concentrations of 135±1.8 mEq/L, 140±1.5 mEq/L, and 152±1mEq/L respectively (p<0.05). There was an increase in oxidative stress in the aorta from values of 36.22±4.64 mU/mg SOD and 0.131±0.013 pg/mL MDA in the control group, to 47.11±4.89 mU/mg SOD and 0.291±0.022 pg/mL MDA in the 8% group (p<0.05 in both cases). The same was observed in the heart, where values were: 174.6125.26 mU/mg SOD, 0.026±0.007 pg/mL MDA in controls, and 259.22±21.98 mU/mg SOD, 0.215±0.073 pg/mL MDA in 8% group (p<0.05 both cases). Increased ERK in aortic tissue, values of 0.29±0.03 pg/mL in controls, 2.68±0.18 pg/mL in 4% group and 3.97±0.68pg/mL in 8% group (p<0.05) suggest increased inflammation. We conclude that the elevation in serum Na+ concentration that follows Na+ ingestion leads to increased oxidative stress and inflammation.

scholarly journals Aplikasi Digital Risalah Ilmu Tajwid dalam Meningkatkan Kemampuan Baca Alquran

2018 ◽  
Vol 11 (2) ◽  
pp. 145-157
Author(s):  
Khairul Khairul ◽  
Muhammad Haramain
Keyword(s):  
Learning Environment ◽  
Research Design ◽  
Empirical Data ◽  
Control Group ◽  
Control Group Design ◽  
Text Book ◽  
Research Results ◽  
Group Design ◽  
Before And After ◽  
The Difference

This article discusses the using of risalah of tajwid digital apps in increasing the students of SMA Negeri 4 Pinrang abilities in reading al-Qur’an. The using of risalah of tajwid digital apps was by optimazing the technology of computer that running by risalah of tajwid digital apps, that able to create the learning environment to prepare the options that can stimulated the students to use the potential cognitive. The objectives of this research was to get the empirical data about the students’ ability in reading Alquran before and after taught by using risalah digital of tajwid science and to get the empirical data about students’ ability in reading Alquran before and after using text book as well as to get the information about the difference in students’ level of ability in reading Alquran at the experiment class and the control class. The research design is used nonequivalent control group design, it means that in this design the experiment class nor the control class was not selected randomly.The research results are: (1) The level of students’ ability in reading Alquran in the experiment class before the treatment was classified in poor category, however, after given the treatment it was in good category. (2) The level of students’ ability in reading Alquran in the control class before the treatment was in poor category, however, after given the treatment it was in good category. (3) There are difference of students’ level ability in reading Alquran in the experiment class and the control class.

scholarly journals PENGARUH MODEL PROBLEM BASED LEARNING BERBASIS LINGKUNGAN TERHADAP HASIL BELAJAR SISWA KELAS X AP PADA MATA AJAR SANITASI, HYGIENE DAN KESELAMATAN KERJA DI SMK PARIWISATA TRIATMA JAYA SINGARAJA TAHUN AJARAN 2018/2019

2019 ◽  
Vol 10 (2) ◽  
pp. 74
Author(s):  
Yulianitias Yulianitias ◽  
Cokorda Istri Raka Marsiti ◽  
Luh Masdarini
Keyword(s):  
Random Sampling ◽  
Model Problem ◽  
Problem Based Learning ◽  
Workplace Safety ◽  
Control Group ◽  
Control Group Design ◽  
The Mean ◽  
The Difference ◽  
Level Of Significance ◽  
Academic Year

ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui perbedaan dari penggunaan model Problem Based Learning berbasis lingkungan terhadap hasil belajar siswa kelas X-AP mata ajar Sanitasi, Hygiene dan Keselamatan kerja di SMK Pariwisata Triatma Jaya Singaraja Tahun Ajaran 2018/2019. Desain penelitian yang digunakan adalah Non equivalent Only Control Group Design. Objek dalam penelitian ini terdiri dari dua kelas sampel, yaitu kelas eksperimen dan kelas kontrol. Metode pemilihan sampel penelitian adalah dengan teknik random sampling. Proses pengumpulan data menggunakan instrumen tes untuk mengetahui hasil belajar siswa, kemudian melakukan pengujian hipotesis dengan menggunakan teknik uji-t. Berdasarkan hasil analisis data, diperoleh thitung 7,641 dan ttabel (pada taraf signifikansi 5%) = 3,460. Hal ini berarti bahwa thitung> ttabel, sehingga dapat diinterpretasikan bahwa terdapat pengaruh yang signifikan terhadap hasil belajar siswa X AP Di SMK Pariwisata Triatma Jaya Singaraja antara kelompok siswa yang dibelajarkan dengan Problem Based Learning berbasis lingkungan lebih tinggi dibandingkan kelompok siswa yang dibelajarkan dengan metode konvensional. Dilihat dari hasil perhitungan rata-rata hasil belajar Sanitasi, Hygiene dan Keselamatan kerja kelompok eksperimen adalah 81 lebih besar dari rata-rata hasil belajar Sanitasi, Hygiene dan Keselamatan kerja kelompok kontrol yaitu 76 Kata kunci: Hasil belajar Sanitasi, Hygiene dan Keselamatan Kerja, Model Problem Based Learning (PBL) Berbasis Lingkungan ABSTRACT The study aimed at finding out the difference of implementation of problem based learning model with environmental basis towards the achievement of class XAP students on the subjects of sanitation, hygiene, and workplace safety at SMK Pariwisata Triatma Jaya Singaraja on the academic year 2018/2019. The research was designed with non equivalent only group control. The objects were two classes of samples, namely controlled and sample classes. Random sampling method was apllied to determine the sample of the research. The data were gathered by using test to find out the achievement of the students , then examining the hypothesis was done by implementing t-examiner technique. The results show that tvalue is 7,641 and ttable (at level of significance 5%) = 3,460. It means that tvalue> ttable. It can be interpreted that there is a significant effect towards the students’ahievement of class X AP at SMK Pariwisata Triatma Jaya Singaraja. The group of students that were treated with Problem Based Learning with the environmental basis got the mean score 81 for sanitation, hygiene, and workplace safety, it is higher than those who were treated with conventional method (controlled group).Key words : achievement, sanitation, hygiene, and workplace safety Model

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