Application of crude natural enzymes for extraction of Wali seed [Brucea javanica (L) Merr]

Abstract Objectives Level of βhCG and the presence of any uterine mass of hydatidiform mole need a careful review or monitoring in order to prevent metastasis, provide an early treatment and avoid unnecessary chemotherapy. Case presentation A 36-year old fifth gravida patient who had a missed abortion was diagnosed as having a molar pregnancy with beta human chorionic gonadotrophin (βhCG) level of 509,921 IU/L. Her lung field was clear and she underwent suction and curettage (S & C) procedure. However, after six weeks, AA presented to the emergency department with a massive bleeding, although her βhCG level had decreased to 65,770 IU/L. A trans-abdominal ultrasound indicated the presence of an intra-uterine mass (3.0 × 4.4 cm). Nevertheless, her βhCG continued to show a declining trend (8,426 IU/L). AA was advised to undergo a chemotherapy but she refused, citing preference for alternative medicine like herbs instead. She opted for an “at own risk” (AOR) discharge with scheduled follow up. Subsequently, her condition improved with her βhCG showing a downward trend. Surprisingly, at six months post S & C, her βhCG ameliorated to 0 IU/L with no mass detected by ultrasound. Conclusions Brucea javanica fruits, Pereskia bleo and Annona muricata leaves can potentially be useful alternatives to chemotherapy and need further studies.

Download Full-text

Effect of injection of brucea javanica oil emulsion plus chemoradiotherapy for lung cancer: a review of clinical evidence

Journal of Evidence-Based Medicine ◽

10.1111/jebm.12001 ◽

2012 ◽

Vol 5 (4) ◽

pp. 216-225 ◽

Cited By ~ 23

Author(s):

Yan-li Nie ◽

Ke-xin Liu ◽

Xuan-yue Mao ◽

Yu-lin Li ◽

Jing Li ◽

...

Keyword(s):

Lung Cancer ◽

Clinical Evidence ◽

Oil Emulsion ◽

Brucea Javanica ◽

Brucea Javanica Oil

Download Full-text

Effect of FOLFOX4 combined with Brucea javanica emulsion on VEGF in patients with gastric cancer

Oncology Letters ◽

10.3892/ol.2017.7429 ◽

2017 ◽

Author(s):

Zheng Chen ◽

Zhenyu Zhou ◽

Zhigang Hu ◽

Qiaodong Xu ◽

Jie Wang

Keyword(s):

Gastric Cancer ◽

Brucea Javanica

Download Full-text

Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

Journal of Ethnopharmacology ◽

10.1016/j.jep.2013.03.034 ◽

2013 ◽

Vol 147 (2) ◽

pp. 442-446 ◽

Cited By ~ 21

Author(s):

Jianhong Yang ◽

Shucai Li ◽

Caifeng Xie ◽

Haoyu Ye ◽

Huan Tang ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Inflammatory Activity ◽

Brucea Javanica ◽

Anti Inflammatory

Download Full-text

Brucea javanica Oil Induces Apoptosis in T24 Bladder Cancer Cells via Upregulation of Caspase-3, Caspase-9, and Inhibition of NF-κB and COX-2 Expressions

The American Journal of Chinese Medicine ◽

10.1142/s0192415x10008093 ◽

2010 ◽

Vol 38 (03) ◽

pp. 613-624 ◽

Cited By ~ 20

Author(s):

Guo-Guang Lou ◽

Hang-Ping Yao ◽

Li-Ping Xie

Keyword(s):

Caspase 3 ◽

Flow Cytometric Analysis ◽

Morphological Characteristics ◽

Control Group ◽

Caspase 9 ◽

Brucea Javanica ◽

Flow Cytometric ◽

T24 Cells ◽

Bladder Cancer Cells ◽

Cox 2

The potential molecular mechanism of Brucea javanica oil in the induction of apoptosis of T24 bladder cancer cells was investigated in vitro. T24 cells were divided into two groups: one, treated with B. javanica oil and the other, untreated. The cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS) and 4 mM glutamine. The morphological characteristics of T24 cells were examined microscopically at the 2nd and 5th day of the culture. The drug toxicity spectrum ( IC 50) was estimated by the MTT assay, and viability of T24 cells was assessed on the basis of the percentage of T24 apoptotic cells, as determined by Annexin/PI staining and flow cytometric analysis. The expression of caspase-3, capase-9, NF-κB p65, and COX-2 was analyzed by Western blotting. Morphological characteristics of the cells on the 2nd day showed apoptosis of the treated T24 cells; it was more apparent in the cells on the 5th day. B. javanica oil decreased the cell viability at the testing concentrations spectrum (5–0.156 mg/ml), and this viability was significantly higher as compared to the control group. In this concentration spectrum, B. javanica oil also induced apoptosis of T24 cells, which was analyzed by annexin/PI staining and flow cytometric analysis. These results were also statistically significant as compared to those of the control group. The expressions of caspase-3 and caspase-9 were low in the control T24 cells, while the expressions of NF-κB and COX-2 were high in normal T24 cells. Treatment with B. javanica oil significantly induced the expressions of caspase-3 and caspase-9 proteins in T24 cells, whereas the expressions of NF-κB and COX-2 proteins were inhibited. B. javanica oil significantly reduced the viability of T24 cells and induced T24 cell apoptosis. The molecular mechanism underlying these effects may be the activation of caspase apoptotic pathway by upregulation of the expression of caspase-3 and caspase-9 proteins and inhibition of the expression of NF-κB and COX-2 proteins.

Download Full-text

Brusatol-Enriched Brucea javanica Oil Ameliorated Dextran Sulfate Sodium-Induced Colitis in Mice: Involvement of NF-κB and RhoA/ROCK Signaling Pathways

BioMed Research International ◽

10.1155/2021/5561221 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Xinghan Zheng ◽

Liting Mai ◽

Tongtong Wang ◽

Ying Xu ◽

Zireng Su ◽

...

Keyword(s):

Signaling Pathways ◽

Activity Index ◽

Disease Activity Index ◽

Protective Effects ◽

Intestinal Epithelial ◽

Colon Tissue ◽

Epithelial Barrier Function ◽

Brucea Javanica ◽

Tnf Α ◽

Brucea Javanica Oil

Brucea javanica oil (BJO) is beneficial for the treatment of ulcerative colitis (UC), and that quassinoids in particular brusatol are bioactive components. However, it is still uncertain whether or not other components in BJO, such as oleic acid and fatty acids, have an anti-UC effect. The present study is aimed at comparing the anti-UC effects between brusatol-enriched BJO (BE-BJO) and brusatol-free BJO (BF-BJO) and at exploring the effects and mechanisms of BE-BJO on colon inflammation and intestinal epithelial barrier function. Balb/C mice received 3% (wt/vol) DSS for one week to establish the UC model. Different doses of BE-BJO, BF-BJO, or BJO were treated. The result illustrated that BE-BJO alleviated DSS-induced loss of body weight, an increase of disease activity index (DAI), and a shortening of colon, whereas BF-BJO did not have these protective effects. BE-BJO treatment improved the morphology of colon tissue, inhibited the production and release of TNF-α, IFN-γ, IL-6, and IL-1β in the colon tissue, and reversed the decreased expressions of ZO-1, occludin, claudin-1, and E-cadherin induced by DSS but augmented claudin-2 expression. Mechanistically, BE-BJO repressed phosphorylation of NF-κB subunit p65, suppressed RhoA activation, downregulated ROCK, and prevented phosphorylation of myosin light chain (MLC) in DSS-treated mice, indicating that the protective effect of BE-BJO is attributed to suppression of NF-κB and RhoA/ROCK signaling pathways. These findings confirm that brusatol is an active component from BJO in the treatment of UC.

Download Full-text

Efficacy of Aidi Injection and Brucea javanica Oil Emulsion Injection in Rectal Cancer during CapeOX Adjuvant Chemotherapy

BioMed Research International ◽

10.1155/2021/2033353 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Wenjun Meng ◽

Xiaoge Zeng ◽

Yuchen Gao ◽

Qi Chen ◽

Lian Bai

Keyword(s):

Rectal Cancer ◽

Adjuvant Chemotherapy ◽

Control Group ◽

Oil Emulsion ◽

Brucea Javanica ◽

Significant Difference ◽

Hand Foot Syndrome ◽

Aidi Injection ◽

One Year ◽

Brucea Javanica Oil

Background. Adjuvant chemotherapy with CapeOX regimen is widely used in resected rectal cancer, which brings benefits to patients. But drug-related toxicities are severe during this process; thus, survival outcomes may potentially be affected. This study explored the efficacy of two Chinese herbal injections, Aidi injection (ADI) and Brucea javanica oil emulsion injection (BJOEI), as adjuvant drugs in CapeOX adjuvant chemotherapy on rectal cancer patients. Methods. A total of 240 cases were enrolled in this retrospective study. 80 cases received CapeOX with ADI (the ADI group), 80 cases received CapeOX with BJOEI (the BJOEI group), and the rest 80 cases received CapeOX alone (the control group). After four cycles’ chemotherapy, adverse reactions (ADRs) and quality of life (QOL) were analyzed. Then, patients received follow-up for at least one year, and the endpoint was disease-free survival (DFS). Results. All patients completed at least four cycles’ adjuvant chemotherapy. The incidence of leukopenia and thrombocytopenia was significantly lower in the ADI group; the incidence of nausea was significantly lower in the BJOEI group; the incidence of hand-foot syndrome was significantly lower in both the ADI group and BJOEI group. Significant difference was found in the control group regarding the Karnofsky Performance Status (KPS) scores prior and posttreatment. No difference was found among three groups regarding one-year DFS. Conclusion. As adjuvant drugs for rectal cancer during CapeOX chemotherapy, ADI shows advantages in decreasing leukopenia and thrombocytopenia, while BJOEI results better in remitting nausea. Both two CHIs had positive impacts on decreasing hand-foot syndrome and the maintenance of patients’ QOL. It is worthy of further study and promotion for CHIs.

Download Full-text

Uji Aktivitas Anti-inflamasi Dan Daya Hambat Siklooksigenase-2 ekstrak etanol Daun Malur (Brucea javanica (L.) Merr.)

Journal of Pharmaceutical And Sciences ◽

10.36490/journal-jps.com.v3i1.33 ◽

2020 ◽

Vol 3 (1) ◽

pp. 7-13

Author(s):

Ifora Ifora

Keyword(s):

Brucea Javanica ◽

Cox 2

Inflamasi merupakan suatu respon protektif normal terhadap kerusakan jaringan yang dimediasi oleh enzim siklooksigenase-2 (COX-2). Penelitian ini bertujuan untuk melihat aktivitas antiinflamasi ekstrak etanol daun malur (Brucea javanica (L.) Merr.) dan daya hambatnya terhadap enzim COX-2. Hewan yang digunakan pada penelitian ini ialah tikus putih jantan yang dibagi menjadi 5 kelompok yaitu dosis 250 mg/kg BB, dosis 500 mg/kg BB, pembanding (Celecoxib), kontrol positif dan kontrol negatif. Pengujian aktivitas antiinflamasi dilakukan dengan cara menginduksi telapak kaki tikus dengan karagen kemudian diukur volume udemnya menggunakan pletismometer dan pengukuran daya hambat COX-2 menggunakan microplate readers. Hasil uji analisis menunjukkan bahwa ekstrak etanol daun malur dosis 250 mg/kg BB dan 500 mg/kg BB memiliki aktivitas antiinflamasi secara signifikan (p < 0,05). Dosis 500 mg/kg BB memiliki daya hambat terhadap COX-2 secara signifikan (p < 0,05) sedangkan dosis 250 mg/kg BB tidak memiliki daya hambat terhadap COX-2 secara signifikan. Dapat disimpulkan bahwa ekstrak etanol daun malur memiliki efek antiinflamasi pada dosis 250 mg/kg BB dan 500 mg/kg BB dan daya hambat terhadap COX-2 pada dosis 500 mg/kg BB.

Download Full-text