scholarly journals Biosynthesis of Silver Nanoparticles Using Calendula officinalis (L.) Extract and Evaluating their Antioxidant Activity

2021 ◽  
Vol 735 (1) ◽  
pp. 012073
Author(s):  
Kareem Talib Khshan ◽  
Hider Abass Alkafaje
Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 559 ◽  
Author(s):  
Bragueto Escher ◽  
Cardoso Borges ◽  
Sousa Santos ◽  
Mendanha Cruz ◽  
Boscacci Marques ◽  
...  

Edible flowers have been used as ingredients because of their biological activities, taste, and overall appearance. This research was aimed to characterize the chemical composition and in vitro antioxidant activity of the marigold flower (Calendula officinalis L.) extracted with different proportions of water and ethyl alcohol, and the lyophilized extract with higher content of antioxidant compounds was incorporated into an organic yogurt. Results showed that the hydroalcoholic extract (50:50 v/v) presented the highest total phenolic content (TPC), flavonoids, and antioxidant activity (ferric reducing antioxidant power (FRAP), total reducing capacity (TRC), and Cu2+/Fe2+ chelating ability). Phenolic acids and flavonoids were quantified in the extract by LC-DAD, while 19 compounds were tentatively identified by ESI-MS/MS. The lyophilized marigold extract (LME) also inhibited 12% of Wistar rat’s brain lipid oxidation in vitro, inhibited α-amylase, and α-glucosidase activities, but showed no cytotoxicity towards cancerous cells (HCT8 and A549). However, marigold flower extract protected human erythrocytes against mechanical stress. When added into an organic yogurt model (0 to 1.5%), LME increased TPC and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH) and TRC), and the sensory analysis showed that the organic yogurt had an acceptance of 80.4%. Our results show that the use of LME may be a technological strategy to increase the content of bioactive compounds in yogurts.


Microwave-assisted hydrodistillation (MAHD) was used as an ecofriendly method to extract the essential oils from flowers and leaves of Calendula officinalis L. cultivated in Algeria. The results obtained were compared with the conventional extraction method, hydrodistillation (HD), and analyzed by gas chromatography-flame ionization detector (GC-FID) and GC-MS. For flowers oils, 33 compounds were identified with HD method vs 20 compounds with MAHD method. For leaves, 26 compounds were identified with HD method vs 19 compounds identified with MAHD method. It is interesting to note, furthermore, that the use of MAHD method during 90 min allowed us to obtain relatively similar yields than HD method during 180 min. The main abundant volatile constituent was αcadinol with 31.9±0.71% for HD vs 39.7±0.26% for MAHD in leaves oils and 32.3±0.26% for HD vs 37.1±0.30% for MAHD in flowers oils. The oxygenated sesquiterpens was the most represented group of natural compounds contributing to the chemical composition in all oils. In the other hand, extraction of total phenolic compounds (TPC) and total flavonoids (TFC) was affected by the solvent type and, thus, 100% methanol was the better extraction solvent for both leaves and flowers. Highest levels were obtained from leaves. The highest antioxidant activity was recorded for leaves extract with 100% methanol. These values indicated a weak antioxidant activity compared to antioxidant standards. A correlation was established between the phenolic and flavonoids contents and the antioxidant activity of the crude extracts. A moderate to great antibacterial activity was observed against Gram+ bacteria. Any antibacterial activity was detected against fungi strains and Gram- bacteria.


2021 ◽  
Vol 20 (1) ◽  
pp. 27-36
Author(s):  
Hassan Bayat ◽  
Mohammad Hossein Aminifard

Selenium (Se) seed priming is an effective method for enhancing seed performance and improving tolerance of crops to abiotic stresses particularly drought. A pot experiment was conducted to determine the effect of seed priming of pot marigold (Calendula officinalis L.) with Se on growth, physiology and antioxidant activity grown under both control and drought stress conditions. Treatments included 6 levels of seed priming with Se (0 (control), 0.5, 1, 1.5, 2 and 4 mg. L–1) and 2 levels of water stress (well-watered and drought conditions). The results showed that supplemental Se at lower concentrations improved growth parameters like total leaf area, root length and total biomass of control and drought-stressed plants. In addition, relative water content and chlorophyll content of the drought-stressed plants increased with the application of Se at lower concentrations. Treatment with Se mitigated adverse effects of drought stress through enhancement of photosynthetic pigments, improvement of water relations, accumulation of soluble sugars and increased antioxidant activity. Seed priming with Se also increased total phenols, flavonoids and free radical scavenging activity of pot marigold plants both under well-watered and water stress conditions. It was found that seed priming with Se at lower concentrations (especially 1.5 mg. L–1) can mitigate the adverse effects of drought stress and improved antioxidant system of pot marigold plants.


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