scholarly journals Biocontrol of Meloidogyne sp. on Tomato Plants by Selected Bacillus spp

2021 ◽  
Vol 757 (1) ◽  
pp. 012019
Author(s):  
T Habazar ◽  
Winarto ◽  
Obel ◽  
Y Yanti ◽  
MR Dani ◽  
...  
2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Vina Maulidia ◽  
Loekas Soesanto ◽  
Syamsuddin Syamsuddin ◽  
Khairan Khairan ◽  
Takahiro Hamaguchi ◽  
...  

Abstract. Maulidia V, Soesanto L, Syamsuddin, Khairan K, Hamaguchi T, Hasegawa K, Sriwati R. 2020. Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.). Biodiversitas 21: 5270-5275. Endophytic bacteria live and colonize in plant tissues without causing disease to their plant host. Among several processes, these bacteria can produce secondary metabolites that can help in the defense of plant host against pathogens. This study aimed to identify endophytic bacteria as biocontrol agents against Meloidogyne sp. in tomato plants. Six endophytic bacteria candidates from the genus Pseudomonas, Arthrobacter, Bacillus, and Serratia were isolated from Solanum Lycopersicum, Psidium guajava, Pinus merkusii, Dendrocalamus asper, Albizia chinensis, and Theobroma cacao L, respectively. The average mortality of Meloidogyne sp. by endophytic bacteria was 70,27% to 95,46%. From these, B. thuringiensis AK08 produced compounds of the secondary metabolites such as flavonoid, phenol, tannins, terpenoids, steroids, saponins, and alkaloids. The best result of the average incubation period, number of galls in the root, number of nematodes at the root, and the number of nematodes in the soil on tomato plant were shown by B. thuringiensis. The major compounds in GC-MS analysis of B. thuringiensis were cholest-5-en-3-ol (3.beta.)-carbonochloridate (25.35%). Bacillus thuringiensis not only has rules as bio-insecticide but also has nematicidal effect.


2021 ◽  
Vol 883 (1) ◽  
pp. 012027
Author(s):  
G N C Tuhumury ◽  
J V Hasinu ◽  
H Kesaulya

Abstract Ralstonia solanocearum is a pathogenic bacteria that attacks tomatoes and causes wilt disease. Many efforts have been made to control this disease through cultivation, use of chemical pesticides, and development of resistant varieties, but bacterial wilt disease remains a serious problem economically. Nowadays, many biological controls are being developed using microbes. The use of Bacillus spp as an unfriendly microbe is very potential to control because it has pathogenic inhibitory activity. This study aims to obtain bacterial isolates of Bacillus spp which can suppress the development of bacterial wilt disease in tomato plants. The research was conducted in vitro at the Laboratory of Plant Physiology in the Agriculture Faculty, Unpatti. The results showed that Bacillus niabensis strain PT-32-1 and Bacillus subtilis strain SW116b could inhibit Ralstonia solanacearum, wilt disease in tomato plants in vitro.


REBIOL ◽  
2020 ◽  
Vol 40 (1) ◽  
pp. 39-45
Author(s):  
Lizbeth Armas-Mantilla ◽  
Yuli J. Barrios-Cárdenas ◽  
Jackson I. Briceño-Muñoz ◽  
Iris Alzugaray-Agreda ◽  
Santos N. Murga-Gutiérrez

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SANOJ KUMAR ◽  
BINDHYA CHAL YADAV ◽  
ATUL TIWARI

We have conducted survey of vegetable fields infested by root-knot nematodes on tomato plants from various regions of Uttar Pradesh. In this paper we are presenting the results of the survey conducted over more than two years in cropping season of tomato plants in Fatehabad. In our study we have examined more than 600 root samples to ascertain the nematode prevalence in a particular area. The purpose of this study is to access the distribution of rootknot nematodes and their incidence on tomato plants.


2015 ◽  
Vol 5 (1) ◽  
pp. 513-522
Author(s):  
Jean-Claude N'ZI ◽  
Lassina FONDIO ◽  
Mako Francois De Paul N’GBESSO ◽  
Andé Hortense DJIDJI ◽  
Christophe KOUAME

Thirty accessions of tomato including twenty eight introduced accessions from The World Vegetable Center-AVRDC and as controls, two commercial varieties Mongal and Calinago, were assessed for agronomic performances at the Experimentation and Production Station of Angud dou of the National Agronomic Research Centre (CNRA) located in the South of Cote d Ivoire. The trial was arranged in a randomized block with three replications. The following parameters were determined at vegetative development stage: plant height at flowering stage, susceptibility of accessions to diseases, day to 50% flowering and day of first harvest, production duration, fruit length, fruit diameter, total number of fruits, number of fruits per plant, potential yield, net yield and fruit damage rate. Results showed that the commercial variety Mongal, with a potential yield of 15.9 and a net yield of 13.1 t ha-1, was the most productive. All the introduced accessions from AVRDC recorded the lowest potential yields from 2.2 to 9.7 t ha-1, and net yields from 1.7 to 8.6 t ha-1. In addition, accessions WVCT8, FMTT847 and WVCT13 were severely infested by bacterial wilt. The reduction of the net yield of tomato accessions resulted in the high fruit damage rates. For the future tomato breeding work, it would be appropriate to introduce into the trials bacterial diseases tolerant varieties. Moreover, some studies could be undertaken to determine the nature of the bacteria involved in the plant wilting and to find out the causal agent of the tomato plants burning at the fructification stage reducing the harvest duration.


Author(s):  
Trần Thị Thu Hà ◽  
Nguyễn Tăng Tôn
Keyword(s):  

Cây hồ tiêu hiện nay đang bị bệnh vàng lá gây hại, một trong những nguyên nhân gây bệnh là do tuyến trùng. Tuy nhiên, việc nghiên cứu về thành phần và mật số tuyến trùng gây hại còn hạn chế và biện pháp phòng trừ bệnh còn gặp nhiều khó khăn. Kết quả nghiên cứu ở rễ và đất trồng hồ tiêu tại Cam Lộ, Quảng Trị xác định được 12 giống tuyến trùng. Tuyến trùng Meloidogyne sp. rất phổ biến, tuyến trùng Pratylenchus sp. và Tylenchus sp. phổ biến, còn 9 giống tuyến trùng khác là ít phổ biến. Mật số tuyến trùng ký sinh thực vật và mật số tuyến trùng Meloidogyne sp. trong đất và rễ cao nhất ở tháng 2 bởi đây là thời điểm mùa mưa thích hợp cho tuyến trùng di chuyển, tìm kiếm thức ăn và sinh sản. Sau đó, tháng 10 và tháng 5 mật số tuyến trùng giảm đáng kể. Phương trình hồi quy tương quan giữa số nốt sưng ở rễ và mật số tuyến trùng Meloidogyne sp. trong rễ vào tháng 2 là y = 0,23x + 10,48 với hệ số tương quan R2 = 0,82 và tháng 5 là y = 1,38x + 7,77 với hệ số tương quan R2 = 0,75 và tháng 10 là y=1,3x + 1,50 với hệ số tương quan R2= 0,83.


Author(s):  
Nguyễn Thị Bích Đào ◽  
Trần Quang Khánh Vân ◽  
Nguyễn Văn Khanh ◽  
Nguyễn Quang Linh

Khi tình hình bệnh hội chứng tôm chết sớm (EMS) đã gây thiệt hại vô cùng to lớn đối với Nuôi trồng thủy sản thì các giải pháp được đề nghị và áp dụng nhằm hạn chế dịch bệnh. Trong đó, việc tìm hiểu và đưa vi khuẩn có lợi để cạnh tranh và ức chế loài vi khuẩn gây bệnh rất được quan tâm, được cho là giải pháp có nhiều triển vọng phù hợp với điều kiện môi trường, đảm bảo sức khỏe cho con người, cũng như hạn chế được dịch bệnh. Đặc biệt, đưa vi khuẩn Bacillus spp. qua đường tiêu hóa của tôm ngay từ khi mới thả đã hạn chế được mật độ vi khuẩn Vibrio. Nghiên cứu này đã phân lập được các chủng Bacillus subtilis B1, Bacillus subtilis B2, Bacillus amyloliquefaciens B4và thử khả năng đối kháng với vi khuẩn Vibrio parahaemolyticus V1 ở các nồng độ 103, 104, 105, 106 CFU theo dõi ở các thời điểm 6h, 12h, 24h, 48h và 72h. Kết quả cho thấy cả ba chủng vi khuẩn Bacillus trên phân lập được đều có khả năng ức chế tốt vi khuẩn Vibrio parahaemolyticus V1, trong đó vi khuẩn Bacillus amyloliquefaciens B4 làtốt nhất với đường kính vòng kháng khuẩn 52,67 ± 4,31mm ở thời điểm 48h; hai chủng Bacillus subtilis B1, Bacillus subtilis B2 lầnlượt là  49,67 ± 3,15 mm, 44,07 ± 5,19 mm, với mức sai số có ý nghĩa thống kê p < 0,05.


2019 ◽  
Vol 31 (1) ◽  
pp. 48-51
Author(s):  
Suha S Hassan ◽  
Nidhal H. Ghaib ◽  
Batool H Al-Ghurabi

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.


Author(s):  
M.N. AL-Rukabi ◽  
◽  
V.I. Leunov

Greenhouse tomatoes are divided into early, medium and late-maturing. The days from seedling germination to the first harvest are taken into account. Tomato has a huge potential for heterosis in terms of precocity, overall yield, signs of resistance and uniformity. The preferred agricultural method is hydroponics, which allows you to grow plants without using soil, only using mineral nutrient solutions in water. The cultivation of tomato plants on the " Fitopyramida " will allow to sell their products in the periods with the highest realized prices. An experiment on variety testing of 11 tomato hybrids of different product groups that differ in precocity allowed us to select the most adapted to the conditions of the " Fitopyramida " technology, including the indeterminate beef Ruddy ball F1, cherry hybrids Elf F1 and orange-fruited cherry Magic harp F1. the determinant hybrid Captain F1 showed Good results.


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