scholarly journals Chemical Component of Kencur (Kaemferia galanga L.) Ethanolic Extract Using Gas Chromatography-Mass Spectrometry

2021 ◽  
Vol 819 (1) ◽  
pp. 012057
Author(s):  
Ni Putu Ermi Hikmawanti ◽  
Supandi ◽  
Lusi Putri Dwita ◽  
Yeni
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Chemical Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals IDENTIFICATION OF BIOACTIVE COMPOUNDS IN FLOWER OF TABERNAEMONTANA DIVARICATA (L.) USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS

2019 ◽  
pp. 129-132
Author(s):  
KALAIMAGAL C
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Tabernaemontana Divaricata ◽  
Chromatography Mass Spectrometry ◽  
Ability To Act

Objective: Herbs are a key resource with therapeutic properties. Nowadays, there is a focus on the identification of bioactive compounds with the ability to act against various disorders. Methods: In the present study, gas chromatography–mass spectrometry analysis was conducted to determine the occurrence of different phytochemical compounds in ethanolic flower extract of Tabernaemontana divaricata (L.). Results: The ethanol extract of flower revealed the presence of several bioactive compounds such as n-hexadecanoic acid, squalene, Vitamin D3, Vitamin A aldehyde, desulfosinigrin, and Urs-12-en-24-oic acid, 3-oxo-, methyl ester, (+)-. Conclusion: The perceived compounds from ethanolic extract of flower have diverse beneficial properties such as antimicrobial, antioxidant, cancer anticipatory effect, pesticide, and antiarthritic.

Phytochemical Screening, Isolation, and Structure Elucidation of the Radish (Raphanus sativus Linn.) Bulb Ethanolic Extract Using Gas Chromatography- Mass Spectrometry (GC-MS)

10.17158/233 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Ma. Eva C. San Juan ◽  
Ramchand S. Jumala ◽  
Karen Hope G. Niasca
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Raphanus Sativus ◽  
Ethanolic Extract ◽  
Bioactive Molecules ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Chromatography Mass Spectrometry ◽  
Long Chain

Nowadays, plant is being used as a biofuel, a probable source for a pesticide and it is valued for its pharmacological effects. Hence, the researchers engaged in the scientific approach to provide explanation to these claims. The study focused on the phytochemical screening of the ethanolic extract of radish bulbs (Raphanus sativus). It further dealt on the isolation and determination of the molecular masses to provide probable structures and associated molecular properties for its constituents.<br />Alkaloids, flavonoids, saponins and tannins were analyzed. Further, this effort involved analysis through Thin Layer Chromatography (TLC) using the mobile phase of Toluene: Chloroform (9:11) and Gas Chromatography- Mass Spectrometry (GC-MS) to analyze the first spot (Retention Factor= 0.667).<br />Phytochemical analysis showed no tannins. Using TLC, it revealed two spots having Rf values of 0.667 and 0.507, respectively. Using GC-MS method, 24 different molecules were isolated and analyzed of which only 23 were given probable structures. The 23 molecules constituted 11 long-chain alkanes, 4 long-chain alkyl esters, 2 aromatic ketones, a benzyl halide, an aryl ether, a quinone, an aromatic aldehyde, a long chain aromatic alkane, and a long-chain alkyl nitrile. With this finding, the bulb extract may be a good source of potential biofuel (mono-alkyl ester), UV A, B and C blockers in the form of diphenyl-ketone, oviposition attractants (long-chain alkanes) and also a source of bioactive molecules such as estragole which shows antispasmodic effect, methyl palmitate with immunosuppressive effect in semi-allografts and hepatoprotective activity in rats, and an analogue of the antidepressant alfetamine.

scholarly journals Gas Chromatography-Mass Spectrometry Analysis ofUlva fasciata(Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Idania Rodeiro ◽  
Sitlali Olguín ◽  
Rebeca Santes ◽  
José A. Herrera ◽  
Carlos L. Pérez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Rat Liver Microsomes ◽  
Ulva Fasciata ◽  
Chromatography Mass Spectrometry ◽  
Aqueous Ethanolic Extract

The chemical composition and biological properties ofUlva fasciataaqueous-ethanolic extract were examined. Five components were identified in one fraction prepared from the extract by gas chromatography-mass spectrometry, and palmitic acid and its ethyl ester accounted for 76% of the total identified components. Furthermore, we assessed the extract’s antioxidant properties by using the DPPH, ABTS, and lipid peroxidation assays and found that the extract had a moderate scavenging effect. In an experiment involving preexposition and coexposition of the extract (1–500 µg/mL) and benzo[a]pyrene (BP), the extract was found to be nontoxic to C9 cells in culture and to inhibit the cytotoxicity induced by BP. As BP is biotransformed by CYP1A and CYP2B subfamilies, we explored the possible interaction of the extract with these enzymes. The extract (25–50 µg/mL) inhibited CYP1A1 activity in rat liver microsomes. Analysis of the inhibition kinetics revealed a mixed-type inhibitory effect on CYP1A1 supersome. The effects of the extract on BP-induced DNA damage and hepatic CYP activity in mice were also investigated. Micronuclei induction by BP and liver CYP1A1/2 activities significantly decreased in animals treated with the extract. The results suggest thatUlva fasciataaqueous-ethanolic extract inhibits BP bioactivation and it may be a potential chemopreventive agent.

scholarly journals Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 85-89 ◽  
Author(s):  
M Padma ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
P Sasikala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Silybum Marianum ◽  
Selective Sequential Extraction ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum. Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry. Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Conclusions: The study established the chemical composition and anticancer activity of the plant. Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds.

scholarly journals PHYTOCHEMICAL STUDIES AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT & ARN.

2017 ◽  
Vol 10 (3) ◽  
pp. 462 ◽  
Author(s):  
Dhivya S M ◽  
Kalaichelvi K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sarcostemma Brevistigma

ABSTRACTObjective: To explore the phytochemical constituents present in Sarcostemma brevistigma using gas chromatography-mass spectrometry (GC-MS).Methods: Preliminary phytochemical analysis of secondary metabolites was made by following standard procedures. GC-MS analysis of the plantextracts was performed by using GC-MS equipped with a DB-35MS capillary standard non-polar column and gas chromatograph interfaced to a MassSelective Detector (MS-DSQ-II) with Xcalibur software.Results: The qualitative phytochemical analysis of this species exhibited the presence of alkaloids, glycosides, flavonoids, phenols, steroids, aminoacids, tannins, terpenoids, quinones, and coumarin. The GC-MS analysis revealed the presence of 24 compounds in the ethanolic extract of aerial partof Sarcostemma brevistigma. The most prevailing compound was 14,17 bis(dimethylaminoamino)[3.3]paracyclophan-5,8-diacetonitrile (14.86%).Conclusion: The study concludes that the species Sarcostemma brevistigma is a potential source for bioactive compounds such as esters, alkanes,alcohols, alkenes, amide, amine, phenol, ketone, and so forth. This study justifies the traditional usage of this species.Keywords: Gas chromatography-mass spectrometry, Sarcostemma brevistigma, Phytochemical compounds, Medicinal plants.

scholarly journals GAS CHROMATOGRAPHY AND MASS SPECTROMETRY OF THE ETHANOLIC EXTRACT OF NEST MATERIAL OF MUD DAUBER WASP, SCELIPHRON CAEMENTARIUM

2018 ◽  
Vol 11 (7) ◽  
pp. 234
Author(s):  
Susheela P ◽  
Rosaline Mary ◽  
Radha R
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Methylene Chloride ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Biological Properties ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: The objective of the present study was to determine the chemical compounds present in the nests of the mud dauber wasp, Sceliphron caementarium.Methods: Gas chromatography-mass spectrometry analysis of the nest samples was carried out by standard procedures. The resultant compounds were compared with the database of the National Institute Standard and Technology (NIST), WILEY8, FAME.Results: The results of the gas chromatography-mass spectrometry analysis of the concentrated ethanol extract revealed the presence of chemical compounds such as methylene chloride, 1, 1’:3’, 1’’-Terphenyl, 5’-Phenyl, Di N Decylsulfone, Eicosanoic acid, 1, 2-Bis (Trimethylsilyl) Benzene, and Androstane-11, 17-Dione, 3-[(Trimethylsilyl) Oxy]-, 17-[O-(Phenylmethyl) O.Conclusion: The compounds identified were found to have biological properties such as anti-inflammatory, antibacterial, and antifungal, and further study of these isolated compounds may prove their medicinal importance in future.

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