scholarly journals Isolation of phosphate-solubilizing rhizosphere fungi from jabon merah (Neolamarckia macrophylla) stand of Sidrap Provenance, South Sulawesi

2021 ◽  
Vol 886 (1) ◽  
pp. 012014
Author(s):  
S W Jufri ◽  
I Rahim ◽  
A Arif ◽  
M Restu ◽  
I Iswanto ◽  
...  
Keyword(s):  
Standard Method ◽  
Point Method ◽  
Soil Minerals ◽  
Ability Test ◽  
Rhizosphere Fungi ◽  
South Sulawesi ◽  
Macro Element ◽  
Phosphate Solubilizing ◽  
Available Phosphate ◽  
Rhizosphere Fungus

Abstract Phosphate is an essential macro element that has a necessary function as a constituent of ATP and DNA in plants. However, the availability of dissolved phosphate in the soil is minimal because it tends to bind with soil minerals to form phosphate complexes. With rhizosphere fungi, the low available phosphate in the soil can be overcome. This study aimed to determine the character and potential of fungi capable of dissolving phosphate. The source of the isolates used was a collection of rhizosphere fungus isolates under the red jabon stand. Purification was carried out using the point method on PDA media. The phosphate dissolving ability test was done using the standard method using liquid pikovskaya media and then analyzed descriptively and quantitatively. Eighteen rhizosphere fungus isolates were observed, two of which could dissolve phosphate, respectively, obtained from isolates JCS16 with a concentration value of 10.48 ppm, JCS 13 with a concentration value of 10.06 ppm.

scholarly journals Improving Cassava Productivity by Soil Bioaugmentation with Phosphate-Solubilizing Actinomycetes and Fungi

2021 ◽  
Vol 16 (2) ◽  
pp. 89
Author(s):  
Alimuddin Ali ◽  
Muhammad Juanda ◽  
A. Farchan SJahid ◽  
Herlina Rante
Keyword(s):  
Gum Arabic ◽  
Field Trials ◽  
In Vitro Test ◽  
In Planta ◽  
South Sulawesi ◽  
Cassava Plant ◽  
Cultivation Technique ◽  
Food Commodities ◽  
Phosphate Solubilizing

<p>Cassava is one of the most important food commodities, but its cultivation technique must be improved, especially the technology in increasing soil fertility. Lack of phosphate (P), one of the major biological nutrients in soil, can reduce<br />agricultural production. Some P-solubilizing microbes can play an important role in increasing the availability of P for plants. The purpose of this study was to evaluate the ability of P-solubilizing microbes isolated from cassava rhizosphere in improving the growth of cassava after soil bioaugmentation with the formula of selected microbes. A total of 50 isolates were successfully isolated from cassava plant rhizosphere collected from several locations in South Sulawesi. In vitro screening on Pikovskaya agar media resulted in six Actinomycetes and six fungal isolates with the best P hydrolysis capacity. One Actinomycetes isolate<br />(Streptomyces sp. A-SDR01) and one fungal isolate (Penicillium sp. F-SKG17) with nonantagonistic effect to each other based on in vitro test were able to improve the vegetative growth of cassava under in planta test. Combination of both isolates in a gum arabic formulation increased plant height and productivity compared to untreated plants when applied as soil bioaugmentation in limited field trials at four locations in South Sulawesi. Therefore, application of P-solubilizing microbes that possess soil bioaugmentation properties is recommended for increasing the growth of cassava plants and their use as<br />microbial biofertilizers should be extended to wider areas.</p>

scholarly journals Phosphate Solubilization Potential of Rhizosphere Fungi Isolated from Plants in Jimma Zone, Southwest Ethiopia

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Firew Elias ◽  
Delelegn Woyessa ◽  
Diriba Muleta
Keyword(s):  
Agricultural Production ◽  
Rhizosphere Soil ◽  
Phosphate Solubilization ◽  
Soil Samples ◽  
Maximum Amount ◽  
Southwest Ethiopia ◽  
Rhizosphere Fungi ◽  
Jimma Zone ◽  
Phosphate Solubilizing ◽  
Diverse Plant

Phosphorus (P) is one of the major bioelements limiting agricultural production. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid Pikovskaya (PVK) medium. A total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and sugarcane. Among the isolates, 167 (46.52%) solubilized inorganic phosphate. The isolated phosphate solubilizing fungi belonged to genera ofAspergillus(55.69%),Penicilliumspp. (23.35%), andFusarium(9.58%). Solubilization index (SI) ranged from 1.10 to 3.05. Isolates designated as JUHbF95 (Aspergillussp.) and JUFbF59 (Penicilliumsp.) solubilized maximum amount of P 728.77 μg·mL−1and 514.44 μg mL−1, respectively, from TCP (tricalcium phosphate) after 15 days of incubation. The highest (363 μg mL−1) soluble-P was released from RP with the inoculation of JUHbF95 in the PVK broth after 10 days of incubation. The present study indicated the presence of diverse plant associated P-solubilizing fungi that may serve as potential biofertilizers.

Phosphate Solubilizing Microorganisms and Phosphate Solubilizing Rhizobium – Mini Review

2013 ◽  
Vol 295-298 ◽  
pp. 2328-2332 ◽  
Author(s):  
Shu Qing Zhang ◽  
Jian Feng Li ◽  
Shang Li Shi ◽  
Ping Hui Huo ◽  
Wu Wu Wen ◽  
...  
Keyword(s):  
Indole Acetic Acid ◽  
Growth And Yield ◽  
Phosphate Deficiency ◽  
Limiting Factor ◽  
Yield And Quality ◽  
P Application ◽  
Phosphate Solubilizing ◽  
The Cost ◽  
Available Phosphate

Phosphorus is the main nutrient element for plant growth, whereas most of phosphate in soil is fixated by calcium, aluminum, iron and organic compounds. Available phosphate deficiency has been the main limiting factor that influences the yield and quality of agricultural products. P solubilizing microorganisms have been widely reported of solubilizing different forms of insoluble phosphates. P solubilizing Rhizobium, especially for strains with the ability of secreting 3-Indole acetic acid, which is also capable of fixating N and inoculation, is found excellent in promoting growth and yield of legume crops, which decreases the cost of agricultural production and the side effect of P application to the environment. The mechanism and amount range of P solubilization for Rhizobium are varied among strains. These results and phenomena from different studies are discussed in this paper.

Sanitary and epidemiological welfare of educational organizations

2020 ◽  
pp. 593-593
Author(s):  
M. Y. Gavryushin ◽  
O. V. Frolova ◽  
O. V. Minko
Keyword(s):  
Standard Method ◽  
Well Being ◽  
The State ◽  
Point Method ◽  
State Of Health ◽  
Educational Organizations ◽  
Hygienic Condition

The sanitary and hygienic condition of educational organizations with the determination of the level of sanitary and epidemiological well-being by the standard method and with the ranking of the values of the influence of factors by the point method was studied. Permissible and potentially dangerous conditions of education are revealed, which indicates the presence of the risk of violations of the state of health of students.

Evaluating and Improving SIMS Method for Measuring Nitrogen in SiC

2006 ◽  
Vol 527-529 ◽  
pp. 617-620 ◽  
Author(s):  
Howard E. Smith ◽  
Kurt G. Eyink ◽  
W.C. Mitchel ◽  
M.C. Wood ◽  
Mark A. Fanton
Keyword(s):  
Standard Method ◽  
Secondary Ion Mass Spectrometry ◽  
Point Method ◽  
Multiple Point ◽  
Industry Standard ◽  
Multiple Data ◽  
Low Levels ◽  
Data Point ◽  
Secondary Ion ◽  
A Current

A multiple data point version of the industry standard, two data point raster-changing procedure is employed to measure low levels (< 1 x 1017 atoms/cm3) of nitrogen (N) in silicon carbide (SiC) by SIMS (Secondary Ion Mass Spectrometry). A current-changing procedure is also employed. Together, these are used evaluate the assumptions of the standard method, to separate and measure the components of background signal, and to improve upon the precision and accuracy of the standard method. The risk of poor precision in the two-point method is demonstrated, as is the improvement provided by the multiple-point method. Results show that, in addition to the wellknown N memory background, adsorption background can contribute significantly to the N signal. In general, establishing the presence of adsorption gas in this way can be used to warn of the presence of ionization background, which is not measurable per se.

scholarly journals Engineered Root Bacteria Release Plant-Available Phosphate from Phytate

2019 ◽  
Vol 85 (18) ◽  
Author(s):  
Christine N. Shulse ◽  
Mansi Chovatia ◽  
Carolyn Agosto ◽  
Gaoyan Wang ◽  
Matthew Hamilton ◽  
...  
Keyword(s):  
High Yield ◽  
Natural Soil ◽  
Soluble Phosphate ◽  
Phosphate Fertilizers ◽  
Content Type ◽  
Generate Plant ◽  
Phytate Hydrolysis ◽  
Phosphate Solubilizing ◽  
Phosphate Source ◽  
Available Phosphate

ABSTRACTMicroorganisms that release plant-available phosphate from natural soil phosphate stores may serve as biological alternatives to costly and environmentally damaging phosphate fertilizers. To explore this possibility, we engineered a collection of root bacteria to release plant-available orthophosphate from phytate, an abundant phosphate source in many soils. We identified 82 phylogenetically diverse phytase genes, refactored their sequences for optimal expression inProteobacteria, and then synthesized and engineered them into the genomes of three root-colonizing bacteria. Liquid culture assays revealed 41 engineered strains with high levels of phytate hydrolysis. Among these, we identified 12 strains across three bacterial hosts that confer a growth advantage on the model plantArabidopsis thalianawhen phytate is the sole phosphate source. These data demonstrate that DNA synthesis approaches can be used to generate plant-associated strains with novel phosphate-solubilizing capabilities.IMPORTANCEPhosphate fertilizers are essential for high-yield agriculture yet are costly and environmentally damaging. Microbes that release soluble phosphate from naturally occurring sources in the soil are appealing, as they may reduce the need for such fertilizers. In this study, we used synthetic biology approaches to create a collection of engineered root-associated microbes with the ability to release phosphate from phytate. We demonstrate that these strains improve plant growth under phosphorus-limited conditions. This represents a first step in the development of phosphate-mining bacteria for future use in crop systems.

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