scholarly journals PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL

2020 ◽  
Vol 8 (3) ◽  
pp. 17
Author(s):  
Kristi Aji Sugiarto ◽  
Desy MH Mantiri ◽  
Nickson J Kawung ◽  
Rizald M Rompas ◽  
Sandra O Tilaar ◽  
...  

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi

2018 ◽  
Vol 5 (3) ◽  
pp. 64
Author(s):  
Aaf Falahudin ◽  
U.I.L. Rahmah

The aim of this research is to get information of heavy metal content of Pb in goat milk fed with vegetables waste silage which is safe to be consumed by society. The experiments were carried out by using 16 pregnant 2 - 2,5 years old goat who were treated until the mother was lactated. The experimental design used was Completely Randomized Design with 4 treatments and 4 replications. The treatments applied were as follows: T0 = 100% forage (mixture of grass and legume), T1 = 80% forage + 20% vegetables waste silage, T2 = 60% forage + 40% silage vegetables waste, and T3 = 40% forage + 60% waste silage vegetables. The experiment was conducted for 5 months. The research results show that the feed silage vegetables waste very real produce milk production daily higher than the control of grass. The provision of silage vegetables waste very real produce metal Pb levels higher than control and be above threshold SNI 3141.1: 2011. So that it can be concluded that the silage vegetables waste not advisable done by farmers due to metal Pb in goat milk is still not safe to be consumed. Keywords : Silage, Vegetables Waste, Pb, Goat Milk.


2021 ◽  
Vol 10 (3) ◽  
pp. 363-368
Author(s):  
Regina Amanda ◽  
Ria Azizah Tri Nuraini ◽  
Endang Supriyantini

Pencemaran air terhadap lingkungan dapat menyebabkan dampak membahayakan yang dapat dirasakan oleh makhluk hidup. Salah satu logam berat yang sering mencemari air adalah logam berat tembaga (Cu). Tujuan penelitian ini untuk mengetahui kemampuan daya serap rumput laut Gracilaria sp. terhadap logam berat Cu dan pertumbuhannya. Penelitian ini menggunakan metode eksperimental laboratoris dengan Rancangan Acak Lengkap (RAL) dengan menggunakan 4 perlakuan penambahan konsentrasi Cu, yaitu kontrol (0,54 ppm, sesuai dengan konsentrasi Cu pada air laut), 3 ppm, 6 ppm, 9 ppm dengan 3 kali pengulangan. Pengukuran kandungan logam Cu terhadap Gracilaria sp. dengan menggunakan Atomic Absorpsion Spektofotometri (AAS). Hasil penelitian menunjukkan bahwa penyerapan logam berat Cu tertinggi terdapat pada konsentrasi 9 ppm, yaitu sebesar 3,63 ppm dan terendah pada kontrol yaitu sebesar 0,27 ppm.. Pertumbuhan Gracilaria sp. tertinggi terdapat pada perlakuan kontrol dengan pertambahan berat sebesar 209,2 gram dan  terendah pada konsentrasi 9 ppm, yaitu sebesar 162,9 gram. Semakin tinggi konsentrasi Cu yang diberikan maka semakin rendah pertumbuhan Gracilaria sp. Water pollution to the environment can cause dangerous impacts that can be felt by living things. One of the heavy metals that often pollutes water is the heavy metal copper (Cu). The purpose of this study was to determine the absorption capacity of Gracilaria sp. to the heavy metal Cu and its growth. This study used a laboratory experimental method with a completely randomized design (RAL) using 4 additional treatments of Cu concentration, namely control (0.54 ppm, according to the Cu concentration in seawater), 3 ppm, 6 ppm, 9 ppm with 3 repetitions. Measurement of Cu metal content against Gracilaria sp. by using Atomic Absorption Spectophotometry (AAS). The results showed that the highest absorption of heavy metal Cu was at a concentration of 9 ppm, which was 3.63 ppm and the lowest was 0,27 ppm in control.. The Growth of Gracilaria sp. The highest was found in the control treatment with a weight gain of 209.2 grams and the lowest was at a concentration of 9 ppm, which was 162.9 grams. The higher the concentration of Cu given, the lower the growth of Gracilaria sp. 


BUANA SAINS ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 21-28
Author(s):  
Aryo Candra Nugroho ◽  
Amir Hamzah ◽  
Hesti Triana Soelistriari

Ultisol is the main type of land in Kalimantan, Sumatra, Papua and Sulawesi, with an area of almost 45.8 million ha or 25% of the land area of Indonesia. Ultisol has a relatively high acidity (average pH 4.5), high Al saturation, reaching 60%. The efforts to increase the fertility of ultisol soils such as the addition of organic materials such as Empty Oil Palm Bunches (OPEFB) and CFA (Coal Fly Ash) or Coal Fly Ash (CFA) improve the physical properties of Ultisol soil. Use of CFA that exceeds the limit can cause pollution especially copper (Cu) and lead (Pb). One way to recover soil from heavy metal contaminants with Phytoremediation technology, mustard (Brassica juncea L.) is one of the plants that easily absorb heavy metals so that it can be used as a Phytoremediation agent. The aims of this research to determine the use of CFA and OPEFB to fix up the ultisol soil and heavy metal content absorbed by plants. This study used Completely Randomized Design with 3 replications. The results showed that using of CFA and OPEFB can increase the available P so that it can increase the growth of mustard plants and absorption of heavy metals. By using the dozes of 54.54g CFA and 18.18g OPEFB (C3K1) can increase P- available at 181.67 m kg-1 . The uptake of heavy metals Cu and Pb in mustard plants is very high. The highest uptake in Cu reached 192.03 mg kg-1 , whereas in Pb it was 80 mg kg-1 . This number is still very high, so it is dangerous if consumed.


2018 ◽  
Vol 6 (1) ◽  
pp. 83-93
Author(s):  
Mochamad Rendy Sety0 Budi ◽  
Boedi Setya Rahardja ◽  
Endang Dewi Masithah

ABSTRACT            Water is an important environmental component for life. Heavy metal water pollution comes from many industries. Heavy Metals Copper (Cu) is one of several other heavy metals that are harmful to living things. One way to anticipate the increased pollution of heavy metals Copper (Cu) in waters is bioremediation using microalgae. This study aims to determine the ability of Spirulina plantesis in absorbing heavy metals Copper (Cu) and to determine the influence of heavy metal Copper (Cu) on the growth of Spirulina platensis. This study used an experimental method with Completely Randomized Design (RAL) consisting of four treatments and five replicates, namely A (S. platensis 0 ppm), B (S. platensis 1 ppm), C (S. platensis 3 ppm), D (S. Platensis 5 ppm). The results showed that Spirulina platensis was able to absorb heavy metal of Copper (Cu) so that it can be used as a heavy metal bioremediation agent. On treatment B (1 ppm) absorption of 87,719%, C (3 ppm) equal to 97,886% and D (5ppm) equal to 95,872 % Growth with the addition of Cu affects Spirulina platensis growthKeywords: Bioremediation, Spirulina platensis, Copper, Growth


2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen


2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.


Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.


2018 ◽  
Vol 12 (1) ◽  
pp. 31-51
Author(s):  
Aprilianto Bayu Arisandy ◽  
Agustina Widyasworo ◽  
Eddy Trijana Sudani

ABSTRACT The research was conducted at Mrs. Sripurwanti's farm in Rejotangan Village, Rejotangan Sub-district, Tulung Agung Regency. The research was conducted from 1 March to 1 April 2017. The aim of the research to determine the difference in the solution of Vitamin B Complex to the results of domestic chicken include hatchability, mortality and hatching weight. Research material using selected 225 eggs of chicken eggs and Vitamin B Complex as many as 30 grains. The research method used is experiments with Completely Randomized Design (RAL). The study was divided into 5 treatments and 5 replications per repeat consisting of 9 eggs. Treatment consists of the addition of Vitamin B complex as much as 0, 45 mg, 90 mg, 135 mg, and 180 mg The results are expected can provide information about alternative moisture enhancer ingredients namely Vitamin B Komplek. The results showed that vitamin B complex was not significant (P> 0.05) to hatchability, live eggs and die 2nd candling , mortality, and hatching weight. Vitamin B complex has significant effect (P <0,05) on hatchability and mortality based on candling to 2. Result of the research can be concluded that giving Vitamin B Complex as much as 45 mg deserves to be additive addition of humidity because it can increase the percentage of hatchability by 15%


2021 ◽  
Vol 888 (1) ◽  
pp. 012054
Author(s):  
A Jaelani ◽  
N Abelina ◽  
R Samudera ◽  
T Rostini ◽  
Masganti ◽  
...  

Abstract This research aims to study and analyze the effect of adding turmeric flour (Curcuma domestica Val) in the rations on the performance of 70 weeks old Isa Brown strain of laying hens. The research method used in this study was an experimental method with a completely randomized design (CRD) step with five treatments and four replications. The number of chickens used was as many as 40 laying hens. The treatments given to the sample were P0 = feed without giving turmeric flour (control), P1 = feed with 0.3% turmeric flour, P2 = feed with 0.5% turmeric flour, P3 = feed with 0.7% turmeric flour and treatment P4 = feed by giving turmeric flour 0,9%. The results showed that giving turmeric flour in the rations of the Isa Brown strain of laying hens affected chicken performance and egg quality. This research concludes that giving turmeric flour a percentage of 0.7% in Isa Brown’s 70-week old laying hens is the best for ration consumption, Hen Day Production, egg weight, feed conversion, and egg quality: Haught Unit, yolk egg color, yolk egg index, grading egg and egg mass. Based on the haugh unit value, all eggs produced are still in the AA grade category


2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.


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