scholarly journals Efficacy of Entomopathogenic Nematodes Against Three Species of Stored Product Insects

2021 ◽  
Vol 910 (1) ◽  
pp. 012047
Author(s):  
Fadel Qader ◽  
Burhan Mohammed ◽  
Husham Ameen
Keyword(s):  
Mortality Rate ◽  
Exposure Time ◽  
Entomopathogenic Nematodes ◽  
Exposure Period ◽  
Red Flour Beetle ◽  
Cowpea Weevil ◽  
Flour Beetle ◽  
Trogoderma Granarium ◽  
Khapra Beetle ◽  
Infectious Stage

Abstract Two specis of entomopathogenic nematodes (EPNs), Steinernema riobravi, symbolized by Sr, and Heterorabditisbacterophora, symbolized by Hb,were tested by using five concentrations: 125, 250, 500, 1000, and 2000 an infectiousphase, ml of the type Sr and four concentrations of 500, 1000, 2000 and 3000infectious phase/mlof the type Hb against Cowpea weevil, Callosobruchus maculates,Khapra beetle, Trogoderma granarium, and Rust red flour beetle Triboliumcastaneum, using three exposure times of 24, 48, and 72 h. The mortalityrate increased withincreasing in concentrations and the exposure period. The results showed that the highest mortality rate was 16.67 whole insects recorded for the 2000 infectious phase/ml with Sr nematodes after 72 hours post- treatment ofC. maculatus. The results showed that the highest rate mortality,which is 20 larvae, was recorded for the infectious phase dose of the genus nematode (Hb), all of which are 500, 1000, 2000 and 3000 gastricphase/ml, and for all exposure periods used between 24 hours and up to 72 hours against the larvae of the T. granarium. The results showed that the highest rate of mortality 10.00 whole insects was recorded for the dose of 3000 Infectious stage/ml and at the exposure time 72 hours, while the lowest rate for this mortality of 1.33 adult insects for rusty flour beetle was recorded for the dose of 500 infectious stage/ml and for the exposure time only 24 hours. The resultsindicated that mortality rate was increasing against any increase in both the exposure time and the dose of nematodes of the genus Hb.

scholarly journals Labroratoryuse of some insecticides and microwave technology in the control of red flour beetle castaneum (Herbst ) (Tenebrionidae: Coleoptera) Tribolium

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Jinan Malik Kalaf
Keyword(s):  
Mortality Rate ◽  
Exposure Time ◽  
Plant Protection ◽  
Adult Mortality ◽  
Red Flour Beetle ◽  
Department Of Agriculture ◽  
Flour Beetle ◽  
Significant Difference ◽  
And Control ◽  
Chemical Pesticides

"The study was carried out to effect of some pesticidesand microwave in red flour beetle in plant protection Department of Agriculture, Basrah University. it was observed that there was a significant difference between the exposure of chemical pesticides and the treatment of control ,as the effect of the larvae death was Deltamethrin pesticide affected the percentage of the destruction of the last larva age and adults stage . The percentage of larval 88.66% compared with the Alpha-cypermethrin and Vapcocidinwere their percentage in which the rate 72.2%and 64.2% respectively. The effect of exposure on the percentage of adult mortality 85.0, 68.8 .53.9% for both Deltamethrin, Alpha-cypermethrin and Vapcocidin respectively. Was superior to the Deltamethrin the mortality rate for larvae and adults 93.53% and 91.5% respectively. The study showed that percentage of mortality of larval and adult increased with increase exposure time and increase intensity of microwave and the highest percentage was at exposure time 20 second, where the mortality rate 100% for the last larva age and adults when the intensity of 800 watts, and did not affect the levels of microwave in the proportion of germination of wheat grain treated as the results of the statistical analysis showed no significant different between treatments and control."

scholarly journals Euphorbia bupleuroides Desf. latex as biopesticide against the red flour beetle (Tribolium castaneum [Herbst, 1797]) and khapra beetle (Trogoderma granarium Everts, 1898)

2021 ◽  
Vol 117 (2) ◽  
pp. 1
Author(s):  
Imene BRIK ◽  
Naama FRAH
Keyword(s):  
Ambient Temperature ◽  
Tribolium Castaneum ◽  
Insecticidal Activity ◽  
Direct Contact ◽  
The Other ◽  
Red Flour Beetle ◽  
Laboratory Evaluation ◽  
Flour Beetle ◽  
Trogoderma Granarium ◽  
Khapra Beetle

<p>Laboratory evaluation of Euphorbia bupleuroides latex as biopesticide against the red flour beetle (Tribolium castaneum) and khapra beetle (Trogoderma granarium) were evaluated at ambient temperature. The insecticidal activity of latex was determined by direct contact application. Different concentrations were prepared by dilution of 2.5, 5.0, 7.0 and 10.0 µl of latex into 0.1 ml acetone. 1 µl was pumped regularly in the thorax of different insects. The latex of E. bupleuroides showed insecticidal activity against T. granarium and T. castaneum. High levels of mortality were associated with the increase in the concentration and time of exposure as well. T. granarium adults are generally more prone to latex insecticidal effects than T. castaneum adults. After 6 days of exposure to E. bupleuroides latex, the LC50 recorded was 14.12 µl for T. granarium adults, and 14.7 µl for T. castaneum. LC90 numbers, on the other hand, were 38.8 µl for the former, and 51.44 µl for the latter.</p>

scholarly journals Effect of High Temperatures on Biological Performance ofTrogoderma granarium (Everts) (Coleoptera: Dermestidae)

2007 ◽  
Vol 4 (3) ◽  
pp. 344-350
Author(s):  
Baghdad Science Journal
Keyword(s):  
High Temperatures ◽  
Exposure Time ◽  
Exposure Period ◽  
Trogoderma Granarium ◽  
Biological Performance

The effect of three high temperatures for five exposure periods on the developments of larvae, pupae and adults of Trogoderma granarium (Everts) and their biological performance were investigated. The results revealed that the percent of mortality was increased as the temperature and the exposure period increased, e. g. exposing last instar larvae to 45°C for 6 hrs caused 100% death of this stage, while exposing adults (1-3) days old to the same temperature and exposure time resulted in that these adults did not able to survive more than 24 hrs.; in addition, the results showed that the ability of reproduction of adults was depended on the temperature, duration of exposure and the sex.

scholarly journals Ovicidal Activity of Essential Oils against Red Flour Beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)

1970 ◽  
Vol 17 ◽  
pp. 57-62 ◽  
Author(s):  
Mina Mondal ◽  
M Khalequzzaman
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Exposure Period ◽  
Red Flour Beetle ◽  
Mortality Data ◽  
Fumigant Toxicity ◽  
Ovicidal Activity ◽  
Flour Beetle ◽  
Ovicidal Effect

Context: The essential oils are being tried as potential candidates for pest and disease management. Several essential oils of botanical origin have been reported for their repellant, toxic and developmental inhibitory activities. The ovicidal effect of essential oil is probably the major factor in the suppression of the development of adults from treated eggs.Objectives: To investigate the ovicidal effect of vapours of five essential oils viz., cardamom (Elletaria cardamomum L.), cinnamon (Cinnamomum zeylanicum Blume), clove (Sygium aromaticum L. Merrill. et. Perry), Eucalyptus spp. and neem (Azadirectica indica A. juss) against the eggs of red flour beetle, Tribolium castaneum (Herbst).Materials and Methods: Ten eggs (24 h old) of T. castaneum were placed in each petridish with wheat flour as food medium and without flour medium and then the petridish were kept inside 650 ml jars with screwed lids. Aliquots of 0.5 ml of each dose of essential oils were applied on filter paper attached to the lower side of the lids. The exposure periods were 24, 48, 72 and 96 h, respectively. After treatment periods, petridishes were taken out of the jars and the final mortality counts were made after 11 days. Mortality data were subjected to probit analysis. Results: The oils had high-fumigant activity against eggs and toxicity progressively increased with increase in exposure time and concentration. At the highest concentration of 5.769 mg/l air and exposure period of 24 h, cinnamon oil achieved 100% mortality in flour and without flour media. The vapours of essential oils from cardamom and clove resulted in 100% mortality of the eggs. Neem oil achieved mortalities as high as 51.66 and 50% mortality at the highest concentration and exposure period in with-flour and without flour medium respectively. At a concentration of 5.769 mg/ l air cardamom oil, the LT90 values were 50.80 and 62.78 h for with-flour and without flour medium respectively.Conclusion: The essential oils of cinnamon and clove, proved to be promising as control agents against stored-product insects, especially T. castaneum.Key words: Essential oil; Fumigant toxicity; Ovicidal activity; Tribolium castaneumDOI: 10.3329/jbs.v17i0.7102J. bio-sci. 17: 57-62, 2009

scholarly journals On the efficiency of entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) on rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Fatma Dolunay Erdoğuş
Keyword(s):  
Biological Control ◽  
Tribolium Castaneum ◽  
Entomopathogenic Nematodes ◽  
Biological Control Agent ◽  
Pure Water ◽  
Control Agent ◽  
Red Flour Beetle ◽  
Flour Beetle ◽  
Control Programs ◽  
Coleoptera Tenebrionidae

Abstract Background The rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) is a serious pest of stored grains and grain products across the world. This beetle is hold a significant place in Turkey by causing damages on stored products. T. castaneum primarily attacks milled grain and its derivates. Entomopathogenic nematodes (EPNs) are regarded as extremely an important biological control agent. EPNs kills their hosts within 48 h by the bacteria they carry. Results Efficacies of 4 isolates of EPNs Steinernema carpocapsae (Tokat Bakisli 05), S. feltiae (Tokat-Emir), Heterorhabditis bacteriophora (TOK-20) and H. bacteriophora (11-KG) against T. castaneum was investigated under laboratory conditions. The experiments were carried out thrice with 10 replicates at 2 different temperatures (15 and 25 ºC). EPNs isolates were tested at 3 different concentrations (250, 500 and 1000 IJs/ml) with a pure water as control. The overall mortality caused by H. bacteriophora (Kg11) was significantly higher than the other EPN species. At 25 ºC, H. bacteriophora (Kg11) at the highest concentration (1000 IJs/ml) caused 87.6% mortality after 120 h., followed by S. carpocapsae and S. feltiae with 79.22 and 75.3% mortality rates, respectively. The mortality percentages exhibited by all nematodes’ species at both temperatures were lowest at the concentration of (250 IJs/ml). At 15 °C, H. bacteriophora (Kg11) caused (55.2%) mortality rate at the highest concentration (1000 IJs / ml) after 120 h. Conclusion The study suggested that these nematodes were efficient and could be recommended to control T. castaneum in its biological control programs.

scholarly journals Compatibility of some botanicals and the entomopathogenic fungus, Beauveria bassiana (Bals.), against the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Habib-ur Rehman ◽  
Amer Rasul ◽  
Muhammad Aslam Farooqi ◽  
Hafiz Muhammad Usman Aslam ◽  
Beenish Majeed ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Tribolium Castaneum ◽  
Plant Extracts ◽  
Larval Mortality ◽  
Red Flour Beetle ◽  
Main Body ◽  
Neem Extract ◽  
Flour Beetle ◽  
Coleoptera Tenebrionidae

Abstract Background The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a serious pest of stored grain commodities worldwide which results in considerable losses to stored wheat, Triticum aestivum (Linn.). Main body The present laboratory bioassay was carried out to examine the efficiency of the fungus, Beauveria bassiana (Bals.) formulation (Racer TM), and Neem extract (NE), Eucalyptus extract (EE), and Tobacco extract (TE) against 3rd instar larvae of T. castaneum. The fungal formulation was applied at 0.3 × 108, 0.6 × 108, and 0.9 × 108 conidia kg−1 of the crushed wheat grains; as well, it was mixed separately with 5% concentrations of each plant extract under the laboratory conditions of 30 ± 2 °C and 65 ± 5% RH. Mortality rate of the tested larvae was enumerated after regular intervals of times. Mortality of the larvae increased at the highest combined concentrations of the fungal conidial formulation and the plant extracts rather than their single treatments. The highest mortality rate of the larvae (71.32%) was recorded at the highest concentration binary mixture of (Racer TM +NE), whereas relatively the lowest mortality rate (15.54%) was enumerated in the combined treatments of (Racer TM +NE). Furthermore, the highest separate concentration of B. bassiana (0.9 × 108 conidia/ml) persistently resulted in more larval mortality (32.68%) of 3rd instar larvae of T. castaneum than the plant extracts (7.52, 9.89, and 14.61%), respectively. A noticeably greater rate of mycosis and sporulation was counted in the larvae of the insect in separate treatments of B. bassiana than in its combined applications with the plant extracts. The highest mycosis (85.13%) and sporulation (160.12 conidia/ml) was detected in the treatment, where the lowest concentration of B. bassiana (0.3 × 108 conidia/ml) was used, alone. Conclusion Hence, it was concluded that integrated use of these two bio-pesticides plus the fungus can be helpful in the integrated pest management program of T. castaneum.

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