scholarly journals In vitro study of anthelmintic effect of butterfly pea (Clitoria ternatea) flower aqueous extract on Tubifex tubifex

2021 ◽  
Vol 912 (1) ◽  
pp. 012015
Author(s):  
A R M Alnaz ◽  
R Ridha ◽  
R F G Nasution ◽  
A H Nasution ◽  
M Ichwan
Keyword(s):  
Experimental Study ◽  
Aqueous Extract ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Tubifex Tubifex ◽  
Clitoria Ternatea ◽  
Traditional Medicines ◽  
Butterfly Pea ◽  
Relationship Of

Abstract Clitoria ternatea (CT) or butterfly pea was one herbs used for Ayurvedic and other traditional medicines. Utilization of its roots, flowers, and leaves proposed several medical adventages with anthelmintic was one of it. Among current anthelmintic burden of resistance, this study aimed to examine the anthelmintic profile from CT flower aqueous extract on Tubifex tubifex. The study was done by conducting a pilot experimental study by extracting CT flowers to water and piping the extracts of different level of dilution to several group of pots containing Tubifex tubifex. Anthelmintic activity was determined with its paralyzing effect and was compared to negative and positive controls with levamisole. Phytochemicals substances were detected with alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids detected. The extract exerts anthelmintic activity it 1:4, 1:40, and 1:400 dilutions and was comparable to 1mg/mL and 0.1mg/mL levamisole. Time to paralysis observed suggested a dose-response relationship of the extract on its anthelmintic activities. It was understood the phytochemicals influents the anthelmintic activity by paralysis worms and leads to death. It was concluded that CT flower aqueous extract presents anthelmintic activity, with further experimental study will needed to be conducted.

scholarly journals In vitro anthelmintic activity of the extract of coffee husk fermented with Pleurotus ostreatus for Ascaridia galli

2017 ◽  
Vol 19 (1) ◽  
pp. 55
Author(s):  
Irma Badarina ◽  
Heru Dwi Putranto ◽  
Endang Sulistyowati
Keyword(s):  
Aqueous Extract ◽  
Pleurotus Ostreatus ◽  
Intestinal Parasites ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Ethanol Solution ◽  
Ascaridia Galli ◽  
Coffee Husk

The objective of  this research was to evaluate the anthelmintic  efficacy of the ethanolic and aqueous extract of coffee husk fermented by Pleurotus ostreatus against Ascaridia galli.  In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm body of Ascaridia galli during 24 hours in petri dish containing different levels of ethanolic and aqueous extract of fermented coffee husk, namely 0%, 1%, 2%, 3% and 4% (w/v) and compared to piperazine 0,5% (w/v) and NaCl 0,9%.   The powdered  samples of fermented coffee husk  were macerated in the 30% ethanol solution  with the ratio samples to solution = 1:3.   In aqueous extract, samples to solution ratio was 1 : 7.  The extract of fermented coffee husk possess phytochemical compunds which responsible for anthelmintic activity.   The anthelmintic activity of ethanolic extract was better than aqueous extract.  Fermented coffee husk ethanolic extract exhibited mortality of A.galli at concentration 2% while showed highest mortality at concentration 4%.  Fermented coffee husk ethanolic extract have the potential to aid in the control of gastro intestinal parasites in animal

scholarly journals The effects of flubendazole and its metabolites on the larval development of Haemonchus contortus (Nematoda: Trichostrongylidae): an in vitro study

2010 ◽  
Vol 47 (4) ◽  
pp. 269-272 ◽  
Author(s):  
H. Bártíková ◽  
L. Skálová ◽  
J. Lamka ◽  
B. Szotáková ◽  
M. Várady
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Lethal Concentration ◽  
Resistant Isolate ◽  
Susceptible Isolate ◽  
Marked Resistance ◽  
Larval Development Test

AbstractThe anthelmintic effects of flubendazole (FLU), its two main metabolites reduced flubendazole (FLU-R) and hydrolyzed flubendazole (FLU-H), and thiabendazole (TBZ) were compared using an in vitro larval development test in two isolates of Haemonchus contortus, a fully susceptible isolate (HCS) and a multi-resistant isolate (HCR). Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99), efficacy factor (EF), and resistance factor (RF). For HCS, both LC50 and LC99 of FLU were lower than those of the reference TBZ. The anthelmintic activity of FLU-R in HCS and HCR was 13 and 6 times lower than the activity of FLU, respectively. The anthelmintic activity of FLU-H was negligible (approximately 363–853 times lower) compared to that of FLU. Although a marked resistance of the HCR isolate to TBZ was confirmed, only a low tolerance to FLU-R and slightly higher tolerance to FLU were found.

Behavior of implant and abutment sets of three different connections during the non-axial load application: An in vitro experimental study using a radiographic method

2021 ◽  
pp. 1-12
Author(s):  
Sergio Alexandre Gehrke ◽  
Berenice Anina Dedavid ◽  
José Manuel Granero Marín ◽  
Luigi Canullo
Keyword(s):  
Experimental Study ◽  
Digital Radiography ◽  
Axial Load ◽  
In Vitro Study ◽  
Vitro Study ◽  
Load Application ◽  
Radiographic Method ◽  
Radiography System ◽  
Torque Values

BACKGROUND: During the masticatory cycle, loads of different intensities and directions are received by the dental structures and/or implants, which can cause micromovements at the junction between the abutment and implant. OBJECTIVE: The objective of this in vitro study was to evaluate the behavior of three different implant connections subjected to different load values using a digital radiography system. Additionally, the torque values for removing the abutment screws were also measured and compared. METHODS: Ninety sets of implant and abutment (IA) were used, divided into three groups according to the type of connection (n = 30 per group): EH group, external hexagon type connection; IH group, internal hexagon connection; and, MT group, Morse taper connection. RESULTS: MT group showed the better vertical misfit behavior at the three intensity of load applied, in comparison with EH and IH groups. In the analysis of torque maintenance (detorque test), MT group showed higher values of detorque when compared with the measured values of EH and IH groups (p < 0.001). CONCLUSIONS: The IA sets of EH and IH groups showed a microgap in all levels of applied loads, unlike the MT group this event was not observed. In the detorque test, MT group increase in the torque values when compared to the initial torque applied, unlike EH and IH groups showed a decrease in the initially torque applied in all conditions tested. A positive correlation was detected between the misfit and detorque values.

Anthelmintic activity of Artemisia annua L. extracts in vitro and the effect of an aqueous extract and artemisinin in sheep naturally infected with gastrointestinal nematodes

2014 ◽  
Vol 113 (6) ◽  
pp. 2345-2353 ◽  
Author(s):  
Aida C. Cala ◽  
Jorge F. S. Ferreira ◽  
Ana Carolina S. Chagas ◽  
Javier M. Gonzalez ◽  
Rodney A. F. Rodrigues ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Artemisia Annua ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Artemisia Annua L

scholarly journals IN VITRO STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS AND THEIR INTERRELATIONSHIP

2018 ◽  
Vol 11 (4) ◽  
pp. 195 ◽  
Author(s):  
Shubhi Rastogi ◽  
Mohammed Shariq Iqbal ◽  
Deepak Ohri
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Cannabis Sativa ◽  
Methanolic Extract ◽  
In Vitro Study ◽  
Strong Positive Correlation ◽  
Total Antioxidant ◽  
Anti Inflammatory

 Objective: The objective of the present work is to study the in vitro anti-inflammatory and antioxidant activity of medicinal plants. The extent and correlation between anti-inflammatory and antioxidant activity have been studied.Method: Methanolic and aqueous extracts of five medicinal plants, namely, Ficus racemosa, Aloe vera, Cannabis sativa, Datura stramonium, and Calotropis gigantean have been taken for in vitro anti-inflammatory and total antioxidant activity.Result: The study showed that the inhibition of protein (albumin) denaturation was maximum in aqueous extract of A. vera with 97.55±1.45%. Proteinase inhibitory action of different plant extracts showed significant action and was found to be maximum in aqueous extract of D. stramonium with 87.89±2.58%. Heat-induced hemolysis showed that maximum inhibition was with aqueous extract of F. racemosa with 90.72±3.33%. When hypotonicity-induced hemolysis activity was done it was found maximum in methanolic extract of C. gigantea with 90.58±3.04%. Anti-lipoxygenase activity was found maximum in methanolic extract of F. racemosa with 94.05±4.24%. When total antioxidant activity was done, it was found highest in F. racemosa (4.38±0.546 mM equivalent of ascorbic acid/g tissue).Conclusion: An overall strong positive correlation between anti-inflammatory and antioxidant activity was observed, indicating that antioxidant activity of the plant species studied might be responsible for their anti-inflammatory property. Further work needs to be undertaken to fully elucidate the antioxidants responsible for anti-inflammatory action and to develop better herbal drug formulations.

Impact of Clitoria ternatea (butterfly pea) flower on in vitro starch digestibility, texture and sensory attributes of cooked rice using domestic cooking methods

2019 ◽  
Vol 295 ◽  
pp. 646-652 ◽  
Author(s):  
Charoonsri Chusak ◽  
Jowynn Ang Yu Ying ◽  
Joseph Lim Zhien ◽  
Porntip Pasukamonset ◽  
Christiani Jeyakumar Henry ◽  
...  
Keyword(s):  
Sensory Attributes ◽  
Starch Digestibility ◽  
Cooking Methods ◽  
Clitoria Ternatea ◽  
In Vitro Starch Digestibility ◽  
Cooked Rice ◽  
Butterfly Pea

scholarly journals In vitro study on anti-oxidant and anti-inflammatory properties of Varnya Mahakashaya Dashemani (aqueous extract): A polyherbal formulation

2018 ◽  
Vol 39 (2) ◽  
pp. 81 ◽  
Author(s):  
Sapna Narasanagi ◽  
MarulaSiddaswamy Mallikarjunaiah Kuppur ◽  
M Shreevathsa ◽  
SunilKumar Ramesh Channarayapatna ◽  
KiniRamachandra Kukkundur ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Polyherbal Formulation ◽  
Anti Oxidant ◽  
Anti Inflammatory

scholarly journals An in vitro Study to determine the Effect of Terminalia chebula Extract and Its Formulation on Streptococcus mutans

2014 ◽  
Vol 15 (3) ◽  
pp. 278-282 ◽  
Author(s):  
Sushma S Nayak ◽  
Sharda C Metgud ◽  
Uday Kumar Bolmal
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Aqueous Extract ◽  
Inhibitory Concentration ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Terminalia Chebula ◽  
Hydroethanolic Extract

ABSTRACT Aim Many weapons are available in the arsenal of a dental professional to combat dental caries, which is almost ubiquitously present. From a public health perspective, most of these weapons are far from being an ideal drug. Hence, there is a demand for better and effective antibacterial agents. This factor stimulated the process of the present study. The aim of the study was to determine the effect of ethanol extract of Terminalia chebula on Streptococcus mutans. Materials and methods Dried ripe fruits of Terminalia chebula were procured and powdered. Physical tests were done to estimate purity of the fruit powder. Hydroethanolic and aqueous extracts were prepared according to standard procedures. Minimum inhibitory concentration of the extracts was determined by tube dilution method and confirmed by agar dilution method. The effect of the hydroethanolic extract on sucrose induced adhesion, glucan-induced aggregation and on glycolysis of Streptococcus mutans was also assessed. Preservative, gelling agent and sweetener were added in suitable quantities to the ethanol extract, and mouthrinse was formulated. Minimum inhibitory concentration of the formulation was also determined. Results Yield was better in case of aqueous extract. The Minimum inhibitory concentration of hydroethanolic extract was determined to be 2.5%. Minimum inhibitory concentration of the aqueous extract was determined to be 10%. Hydroethanolic extract of Terminalia chebula (2.5%) inhibited sucrose induced adherence and aggregation of Streptococcus mutans in vitro. Conclusion The mouthrinse formulated from ethanol extract of Terminalia chebula demonstrated substantial antibacterial activity and could be used as an effective anticaries agent. Clinical Significance Terminalia chebula mouthrinse can be effectively used in clinical practice as an anticaries mouthrinse with additional benefit being that it is safe and economical. How to cite this article Nayak SS, Ankola AV, Metgud SC, Bolmal UK. An in vitro Study to determine the Effect of Terminalia chebula Extract and Its Formulation on Streptococcus mutans. J Contemp Dent Pract 2014;15(3):278-282.

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