scholarly journals Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

2021 ◽  
Vol 912 (1) ◽  
pp. 012052
Author(s):  
D Elfiati ◽  
Delvian ◽  
A Susilowati ◽  
N W Y Rizki ◽  
A F M Harahap ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
Methyl Cellulose ◽  
Economic Benefits ◽  
Soil Samples ◽  
Morphological Identification ◽  
Rhizophora Stylosa ◽  
Cellulolytic Fungi ◽  
Mangrove Soil ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Mangrove forest is one of the forest ecosystems that can be found in tidal areas. Mangrove forest have ecological, socio-economic benefits for organisms that live in the sea. One of the microorganism that live in mangrove forest is fungus. Therefore, this study aimed to isolate and identify phosphate solubilizing and cellulolytic fungi from mangrove soil. Soil samples were collected from a stands of Rhizophora stylosa in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Soil samples were taken randomly with a dept of 0-20 cm. Isolation of phosphate solubilizing fungi was carried out using Pikovskaya medium and cellulolytic fungi using carboxyl methyl cellulose (CMC) medium. To determine the effectiveness of fungi qualitatively, the phosphate solubility and cellulolytic index were calculated. All isolates obtained were identified morphologically by observing colonies macroscopically and microscopically. This study obtained 12 isolates of phosphate solubilizing and 8 isolates of cellulolytic fungi. Phosphate solubility index ranging from 2.06-2.87 and cellulolytic index of 0.33-3.00. Morphological identifications showed that all isolates of phosphate solubilizing fungi belonged to the genus Aspergillus, while all isolates of cellulolytic fungi belonged to the genus Chaetomium.

scholarly journals Characterization of phosphate solubilizing and cellulolytic fungi isolated from soil under Eurycoma longifolia stands

2021 ◽  
Vol 886 (1) ◽  
pp. 012018
Author(s):  
D Elfiati ◽  
A Susilowati ◽  
T M Siagian
Keyword(s):  
Medicinal Plants ◽  
Economic Value ◽  
Bioactive Compound ◽  
Soil Samples ◽  
Morphological Identification ◽  
Cellulolytic Fungi ◽  
Eurycoma Longifolia ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Eurycoma longifolia is one of the potential medicinal plants and has a high economic value if developed. The bioactive compound produced by E. longifolia has potential as medicine, thus causing increased exploitation of their habitat. This condition resulted in the decreasing population of E .longifolia in nature. The distribution of E. longifolia is influenced by soil fertility conditions. The presence of phosphate solubilizing fungi and cellulolytic fungi in the soil can support the growth of E. longifolia in nature. The purpose of this study was to calculate the population of phosphate solubilizing and cellulolytic fungi and to identify the types of phosphate solubilizing and cellulolytic fungi found in the soil under E. longifolia stands. Soil samples were taken randomly at a depth of 0–20 cm in a natural forest of Papaso Village, Lubu Sutam District, Padang Lawas Regency, North Sumatra. Population and isolation of phosphate solubilizing and cellulolytic fungi were obtained by dilution methods. The isolates obtained were identified morphologically to the genus level by macroscopic and microscopic observation of colonies. The results showed that the population of phosphate solubilizing and cellulolytic fungi were 6.64 × 104 CFU/mL and 9.31 × 103 CFU/mL, respectively. The isolation results in 11 isolates of phosphate solubilizing and 10 isolates of cellulolytic fungi. Based on the morphological identification of phosphate solubilizing fungi isolates consisted of 5 genera, namely Aspergillus, Penicillium, Rhizopus, Fusarium, and Mucor, while isolates of cellulolytic fungi also included 5 genera, namely Aspergillus, Cladosporium, Penicillium, Rhizopus, and Mucor.

scholarly journals Mangrove potential and community characteristics for strengthening the utilization of environmental services based on ecotourism: A case study in Karangsong, Indramayu, West Java

2021 ◽  
Vol 914 (1) ◽  
pp. 012021
Author(s):  
R Garsetiasih ◽  
M Takandjandji ◽  
H Gunawan ◽  
V Yuskianti ◽  
E Karlina
Keyword(s):  
Mangrove Forest ◽  
Public Awareness ◽  
Bird Species ◽  
Economic Benefits ◽  
Local Communities ◽  
Local Economy ◽  
Environmental Services ◽  
Rhizophora Stylosa ◽  
Interview Method ◽  
Mangrove Rehabilitation

Abstract Mangrove rehabilitation is often challenged by a lack of support from local communities. The success of mangrove rehabilitation can increase the chances of success by increasing the local economy, which will help increase public awareness. This study was conducted in the mangrove forest of Karangsong Village with the aim to identify the potential of mangroves and the characteristics of local communities as capital for the use of environmental services based on mangrove ecotourism. The interview method with semi-structured guidelines was applied to obtain social, economic and public perceptions data. Karangsong mangrove forest is dominated by three species of mangroves, namely Rhizophora mucronata, Rhizophora stylosa and Rhizophora apiculate. It has also become a habitat for various bird species such as javan pond heron, wandering whistling duck, lesser adjutant, imperial pied pigeon, and common sandpiper, which are potential ecotourism objects. The results showed that positive perceptions derived from the economic benefits obtained from mangroves, which have become feedback for mangrove conservation. The role of the driving group is also crucial to maintain the consistency of stakeholders’ enthusiasm to conserve mangroves. Sustainable ecotourism development needs to involve local communities in its management.

scholarly journals Antibacterial Screening of Bacterial Isolates Associated with Mangrove Soil from the Ngurah Rai Mangrove Forest Bali

2021 ◽  
Vol 10 (2) ◽  
pp. 129-133
Author(s):  
Anak Agung Gede Indraningrat ◽  
Made Dharmesti Wijaya ◽  
Putu Arya Suryanditha ◽  
Ayu Savitri Siskayani ◽  
Ni Made Defy Janurianti
Keyword(s):  
Mangrove Forest ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Subtilis Strain ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Mangrove Soil ◽  
Antibacterial Screening ◽  
Staphyloccocus Aureus ◽  
Antibacterial Potential

In this study we reported cultivation of bacteria associated with mangrove soil from the Ngurah Rai Mangrove Forest, Bali. Mangrove soil samples were serially diluted using sterile artificial seawater, spread onto Starch Casein M agar and incubated at 28oC for 28 days. Cultivation of mangrove soil samples yielded 165 bacterial colonies with 68 isolates were selected and purified based on different morphology. Of these 68 isolates, 22 isolates displayed antibacterial activities ranging from weak to strong inhibition against at least one of four bacterial indicators namely Staphyloccocus aureus, Streptococus mutans, Escherichia coli and Klebsiella pneumoniae using perpendicular streak method. Overall, 19 out of 22 bacteria isolates displayed weak antibacterial potential and two isolates exhibited moderate antibacterial activity. The isolate SA4 was the only bacterium with strong antibacterial potential with measured clear distance ≥ 10 mm against the four bacterial isolates. Sequence analysis based on 16S rRNA gene fragment assigned the isolate SA4 as Bacillus subtilis strain BIL/BS-168. Overall, this study confirmed the untapped potential of antibacterial activities from bacteria associated with mangrove soil.

scholarly journals Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Deni Elfiati ◽  
Delvian DELVIAN ◽  
Hamidah Hanum ◽  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat
Keyword(s):  
Peat Soil ◽  
Selective Medium ◽  
Soil Samples ◽  
Available Phosphorus ◽  
Peat Soils ◽  
Insoluble Phosphate ◽  
Value Range ◽  
Qualitative Test ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

scholarly journals Phosphate solubilizing bacteria isolated from Tuban mangrove soil, Indonesia

2021 ◽  
Vol 762 (1) ◽  
pp. 012007
Author(s):  
Fatimah ◽  
I N Annizah ◽  
D D Alawiyah ◽  
R D Susetyo ◽  
T Surtiningsih ◽  
...  
Keyword(s):  
Phosphate Solubilizing Bacteria ◽  
Mangrove Soil ◽  
Phosphate Solubilizing

scholarly journals Alkaline phosphatase activity of a phosphate solubilizing Alcaligenes faecalis , isolated from Mangrove soil

2017 ◽  
Vol 1 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Bikash Chandra Behera ◽  
Himendra Yadav ◽  
Santosh Kumar Singh ◽  
Bijay Kumar Sethi ◽  
Rashmi Ranjan Mishra ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alcaligenes Faecalis ◽  
Mangrove Soil ◽  
Phosphate Solubilizing

scholarly journals Polyhydroxyalkanoates production by a bacterium isolated from mangrove soil samples collected from Quang Ninh province

2012 ◽  
Vol 3 (2) ◽  
pp. 76-79 ◽  
Author(s):  
Thuoc Van Doan ◽  
Binh Thi Nguyen
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Culture Medium ◽  
Carbon Sources ◽  
Nile Red ◽  
Soil Samples ◽  
Mangrove Soil ◽  
Transmission Electron ◽  
Bacterium Strain ◽  
Quang Ninh

A PHA producing bacterium (strain QN271) was selected from mangrove soil samples collected from Quang Ninh province by using the Nile red dying technique. PHA accumulation in the selected bacterium strain was confirmed by transmission electron microscope. With the exception of maltose or sucrose, the bacterium strain was found to be able to synthesize PHA from various carbon sources (glucose, xylose, fructose, glycerol, and glucose plus propionate). The strain accumulated poly(3-hydroxybutyrate) from glucose, fructose, xylose, and glycerol whereas poly(3-hydroxybutyrate-co-3-hydroxyvalarate) was produced when a combination of glucose and propionate was included in the culture medium. Fructose was found to be most suitable substrate for PHA synthesis by strain QN271. PHA content of 63.3% and CDW of 6 g/L were obtained after 32 hrs of cultivation in fructose medium. Chủng vi khuẩn có khả năng sinh tổng hợp PHA đã được phân lập từ đất rừng ngập mặn tỉnh Quảng Ninh nhờ kỹ thuật nhuộm với Nile red. Ảnh quan sát dưới kính hiển vi điện tử dẫn truyền chứng tỏ rằng chủng vi khuẩn này có khả năng tích lũy lượng lớn PHA trong tế bào. Chủng vi khuẩn tuyển chọn có khả năng sinh tổng hợp PHA từ nhiều nguồn các bon khác nhau như glucose, xylose, fructose, glucerol, glucose và propionate nhưng không có khả năng tổng hợp PHA từ maltose hoặc saccharose. Chủng vi khuẩn tuyển chọn tổng hợp poly (3-hydroxybutyrate) từ các nguồn các-bon như glucose, xylose, fructose, hay glycerol, trong khi đó poly (3-hydroxybutyrate-co-3-hydroxyvalarate) sẽ được tổng hợp khi phối hợp sử dụng hai nguồn các-bon (glucose và propionate). Fructose là nguồn các-bon tốt nhất cho chủng QN271 sinh tổng hợp PHA, khi nuôi cấy trong môi trường có fructose chủng vi khuẩn này có thể tạo ra lượng sinh khối là 6 g/L trong đó có chứa 63.3% PHA sau 32 giờ.

scholarly journals KEANEKARAGAMAN JENIS MANGROVE DI DESA MENDALOK KECAMATAN SUNGAI KUNYIT KABUPATEN MEMPAWAH

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Syahrul Muharamsyah ◽  
M Sofwan Anwari ◽  
Hafiz Ardian
Keyword(s):  
Mangrove Forest ◽  
Avicennia Marina ◽  
Raw Material ◽  
Mangrove Forests ◽  
Bruguiera Gymnorrhiza ◽  
Mangrove Species ◽  
Rhizophora Stylosa ◽  
Sonneratia Caseolaris ◽  
Rhizophora Apiculata ◽  
Bruguiera Cylindrica

Mangrove forests are unique ecosystems that have ecological, biological and socio-economic functions. The function of mangrove forests on the environment is very important especially in the coastal and oceanic regions. Mangrove forests providers of wood, leaves as raw material for medicines, and natural dye. This study aims to inventory the diversity of species of mangrove vegetation in Mendalok Village, Sungai Kunyit Subdistrict, Mempawah Regency. The benefits of this study are to provide the data on mangrove forest vegetation as basic data for local government and related agencies in efforts to protect and preserve mangrove forests in Mendalok Village, Sungai Kunyit Subdistrict, Mempawah Regency. Inventory the tree in mangrove forest used a line with measured 200 meters. There are 6 lines and the distance between the lines as far as 100 meters. The lines of observation are placed by purposive sampling. The results of research found 11 types of species and consisted of 6 genera. The genera are Avicennia, Bruguiera, Ceriops, Rhizophora, Soneratia and Xylocarpus. The species found were Avicennia alba, Avicennia marina, Bruguiera cylindrica, Bruguiera gymnorrhiza, Bruguiera parviflora, Ceriops decandra, Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa, Sonneratia caseolaris, Xylocarpus mollucensis. Diversity of mangrove species in Mendalok Village, Sungai Kunyit Subdistrict, Mempawah Regency was high and should be maintained for conservation and ecotourism area. Keywords : conservation, ecotourism, mangrove, Mendalok Village

scholarly journals Detection Of Community Knowledge Level Of Economic, Ecological Benefits And Causes Of Damage To Mangrove Forest Ecosystems

2021 ◽  
Vol 748 (1) ◽  
pp. 012017
Author(s):  
E Wahyuni ◽  
Zulhafandi ◽  
Hendris ◽  
Jarin
Keyword(s):  
Mangrove Forest ◽  
Forest Ecosystems ◽  
Mangrove Ecosystem ◽  
Economic Benefits ◽  
Household Waste ◽  
Mangrove Forests ◽  
Scoring Method ◽  
Ecological Benefits ◽  
Community Knowledge ◽  
Breeding Sites

Abstract Mangrove forests are natural resources in coastal areas that have an important role in terms of social, economic and ecological aspects. However, the utilization of the mangrove ecosystem makes it vulnerable to damage. This study aims to determine the level of public knowledge of the economic, ecological benefits and damage that happened to mangrove areas in Tarakan City. The scoring method was used to determine the total score or the total score of the respondents’ answers, which amount to 50 people. that the community’s knowledge of the economic benefits of mangrove forests was categorized as know for the benefits of mangroves as firewood, mangrove areas as a place for settlement, mangroves as a place to get fish and mangrove benefits as aquaculture areas with total scores of 226, 200, 232,230 respectively. However, the level of community knowledge about the benefits of mangroves as a medicinal ingredient obtained a total score of 164, which means the level of community knowledge was in the doubtful category. While the level of community knowledge of the ecological benefits of mangrove forests as coastline guards, sea wave barriers, sea wind protectors, and animal breeding sites were included in the category of “Know” with a total score of 228, 224, 234, 240 respectively, but the level of knowledge The community regarding the benefits of mangroves that can manage household waste was in the “doubtful” category with a total score of 128. The community was aware of the damage to mangrove forest ecosystems caused by garbage, logging / mangroves, expanding aquaculture, settlements and increasing population. with a total score of 234,232, 210,228 and 200 levels of knowledge, respectively, which are included in the “Know” category.

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