scholarly journals Characterization of phosphate solubilizing and cellulolytic fungi isolated from soil under Eurycoma longifolia stands

2021 ◽  
Vol 886 (1) ◽  
pp. 012018
Author(s):  
D Elfiati ◽  
A Susilowati ◽  
T M Siagian
Keyword(s):  
Medicinal Plants ◽  
Economic Value ◽  
Bioactive Compound ◽  
Soil Samples ◽  
Morphological Identification ◽  
Cellulolytic Fungi ◽  
Eurycoma Longifolia ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Eurycoma longifolia is one of the potential medicinal plants and has a high economic value if developed. The bioactive compound produced by E. longifolia has potential as medicine, thus causing increased exploitation of their habitat. This condition resulted in the decreasing population of E .longifolia in nature. The distribution of E. longifolia is influenced by soil fertility conditions. The presence of phosphate solubilizing fungi and cellulolytic fungi in the soil can support the growth of E. longifolia in nature. The purpose of this study was to calculate the population of phosphate solubilizing and cellulolytic fungi and to identify the types of phosphate solubilizing and cellulolytic fungi found in the soil under E. longifolia stands. Soil samples were taken randomly at a depth of 0–20 cm in a natural forest of Papaso Village, Lubu Sutam District, Padang Lawas Regency, North Sumatra. Population and isolation of phosphate solubilizing and cellulolytic fungi were obtained by dilution methods. The isolates obtained were identified morphologically to the genus level by macroscopic and microscopic observation of colonies. The results showed that the population of phosphate solubilizing and cellulolytic fungi were 6.64 × 104 CFU/mL and 9.31 × 103 CFU/mL, respectively. The isolation results in 11 isolates of phosphate solubilizing and 10 isolates of cellulolytic fungi. Based on the morphological identification of phosphate solubilizing fungi isolates consisted of 5 genera, namely Aspergillus, Penicillium, Rhizopus, Fusarium, and Mucor, while isolates of cellulolytic fungi also included 5 genera, namely Aspergillus, Cladosporium, Penicillium, Rhizopus, and Mucor.

scholarly journals Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

2021 ◽  
Vol 912 (1) ◽  
pp. 012052
Author(s):  
D Elfiati ◽  
Delvian ◽  
A Susilowati ◽  
N W Y Rizki ◽  
A F M Harahap ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
Methyl Cellulose ◽  
Economic Benefits ◽  
Soil Samples ◽  
Morphological Identification ◽  
Rhizophora Stylosa ◽  
Cellulolytic Fungi ◽  
Mangrove Soil ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Mangrove forest is one of the forest ecosystems that can be found in tidal areas. Mangrove forest have ecological, socio-economic benefits for organisms that live in the sea. One of the microorganism that live in mangrove forest is fungus. Therefore, this study aimed to isolate and identify phosphate solubilizing and cellulolytic fungi from mangrove soil. Soil samples were collected from a stands of Rhizophora stylosa in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Soil samples were taken randomly with a dept of 0-20 cm. Isolation of phosphate solubilizing fungi was carried out using Pikovskaya medium and cellulolytic fungi using carboxyl methyl cellulose (CMC) medium. To determine the effectiveness of fungi qualitatively, the phosphate solubility and cellulolytic index were calculated. All isolates obtained were identified morphologically by observing colonies macroscopically and microscopically. This study obtained 12 isolates of phosphate solubilizing and 8 isolates of cellulolytic fungi. Phosphate solubility index ranging from 2.06-2.87 and cellulolytic index of 0.33-3.00. Morphological identifications showed that all isolates of phosphate solubilizing fungi belonged to the genus Aspergillus, while all isolates of cellulolytic fungi belonged to the genus Chaetomium.

scholarly journals Medicinal Plant by Batak Toba Medicinal plants used by the Batak Toba Tribe in Peadundung Village, North Sumatra, Indonesia

2019 ◽  
Vol 20 (2) ◽  
pp. 510-525 ◽  
Author(s):  
MARINA SILALAHI ◽  
NISYAWATI NISYAWATI ◽  
DINGSE PANDIANGAN
Keyword(s):  
Medicinal Plants ◽  
Curcuma Longa ◽  
Zingiber Officinale ◽  
Single Species ◽  
Psidium Guajava ◽  
Piper Betle ◽  
Structured Interviews ◽  
Medicinal Species ◽  
Eurycoma Longifolia ◽  
North Sumatra

Abstract. Silalahi M, Nisyawati, Pandiangan D. 2019. Medicinal plants used by the Batak Toba Tribe in Peadundung Village, North Sumatra, Indonesia. Biodiversitas 20: 510-525. Research of the medicinal plants by the Toba Batak ethnic has limited, even though the globalization and modernization resulted to degradation of the local knowledge. The objectives of this study were (i) documentation of medicinal plants used in the traditional therapies by the Batak Toba tribe of Peadundung Village, North Sumatra, Indonesia, and (ii) analysing the data by quantitative ethnobotanical tools such as use value (UV), cultural significance index (CSI), relative frequency of citation (RCF) and informant consensus factor (ICF) to determine the cultural importance of medicinal plants in order to develop a tool for their conservation. Semi-structured interviews with 41 identified respondents was the methodology employed for qualitative data collection. A total of 149 medicinal species of plants, belonging to 131 genera and 55 families, were recorded in the study which are used in the treatment of 21 categories of ailments. Plants with the highest UV were Eurycoma longifolia (UV=3.44), Curcuma longa (UV=2.67) and Zingiber officinale (UV=2.60). Eight species, namely Curcuma longa, Eurycoma longifolia, Allium cepa, Psidium guajava, Aleurites moluccanus, Piper betle, Citrus hystrix and Uncaria gambir were found to be having the highest RCF value of 1.00. Eurycoma longifolia (CSI=126), Curcuma longa (CSI=112) and Zingiber officinale (CSI = 105) emerged as the culturally most significant medicinal plants. Thrush and aphrodisiac use categories received the highest ICF of 1.00 each because the informants agreed of using only a single species for each of these categories. Eurycoma longifolia was used as an aphrodisiac whereas Averrhoa carambola was used against thrush. All these important and significant plants suffer the greatest harvesting pressure, hence their conservation should be given priority.

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

scholarly journals Distribution and association pattern of pasak bumi (Eurycoma longifolia) in Batang Lubu Sutam District, Padang Lawas, North Sumatra

2021 ◽  
Vol 912 (1) ◽  
pp. 012056
Author(s):  
A Susilowati ◽  
H H Rachmat ◽  
K S Yulita ◽  
D Elfiati ◽  
I M Ginting
Keyword(s):  
Natural Habitat ◽  
Population Decline ◽  
Economic Value ◽  
Eurycoma Longifolia ◽  
Association Pattern ◽  
Intestinal Worms ◽  
Index Value ◽  
Degree Of Association ◽  
Shorea Leprosula ◽  
North Sumatra

Abstract Pasak bumi (Eurycoma longifolia) is a medicinal plant with various benefits and has long been used to cure various diseases such as cancer, gingivitis, intestinal worms, and anti-inflammatory anti-malarial, and tonic after childbirth. The root has high economic value leading to unsustainable harvest and drastic population decline. As initial data in conservation activities, information regarding the association of pasak bumi in Batang Lubu Sutam natural forest has not been obtained. Therefore, this research was conducted to determine the pasak bumi distribution and its association within their natural habitat in Batang Lubu Sutam forest. The study was conducted by using the vegetation analysis method using a plotted path determined by purposive sampling. Data analysis was performed by calculating association indices, including the Ochiai index (Oi), Dice index (Di), and Jackard index (Ji). The results showed that pasak bumi were found in the seedling and sapling stage in the research location and form a degree of association with several plants. The species that formed the highest degree of association with the pasak bumi at both levels of regeneration was Shorea leprosula with an Ochiai index value of 0.85 at the seedling level and 0.94 at the sapling level.

scholarly journals Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Deni Elfiati ◽  
Delvian DELVIAN ◽  
Hamidah Hanum ◽  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat
Keyword(s):  
Peat Soil ◽  
Selective Medium ◽  
Soil Samples ◽  
Available Phosphorus ◽  
Peat Soils ◽  
Insoluble Phosphate ◽  
Value Range ◽  
Qualitative Test ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.

scholarly journals Exploration, isolation and characterization of indigenous rhizobacteria from patchouli rhizosphere as PGPR candidates in producing IAA and solubilizing phosphate

2022 ◽  
Vol 951 (1) ◽  
pp. 012055
Author(s):  
H Halimursyadah ◽  
Syamsuddin ◽  
Nurhayati ◽  
DN Rizva
Keyword(s):  
Phosphate Solubilization ◽  
Descriptive Analysis ◽  
Soil Samples ◽  
Gram Negative ◽  
Iaa Production ◽  
Isolation And Characterization ◽  
Aceh Province ◽  
Qualitative Descriptive ◽  
Phosphate Solubilizing

Abstract Microorganisms that are active and aggressive colonizing the rhizosphere are known as rhizobacteria. They are able to act as biofertilizers, bioprotectants, biostimulants and bioremediation. This study aims to identify and characterize groups of rhizobacteria present in the patchouli rhizosphere that can produce IAA compounds and have the ability to solubilize phosphate in the soil. Soil samples were taken from the patchouli rhizosphere at Purwosari Village, Nagan Raya, Aceh Province, Indonesia. This study used quantitative and qualitative descriptive analysis through serial dilutions to obtain rhizobacterial strains. Parameters observed were macroscopic and microscopic characteristics, gram test, IAA production and phosphate solubilization. The study obtained 37 isolates of rhizobacteria from Purwosari (PS), comprising 25 isolates of gram positive and 12 isolates of gram negative. The rhizobacteria PS 5/1 produced the lowest IAA at 21.66 ppm, whereas isolate 5/6 C produced the highest IAA at 83.38 ppm. Twenty-five isolates of rhizobacteria could solubilize phosphate while the remaining 12 isolates did not have this ability. The rhizobacteria PS 7/1 resulted in the highest PSI at 2.55 and isolates PS 8/7 produced the lowest PSI at 1.33. The rhizobacteria isolates that can produce IAA and phosphate solubilizing have the potential to be used as PGPR candidates.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Phytochemical characterization of antimycobacterial crude extracts from medicinal plants traditionally used in Mozambique

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
X Luo ◽  
D Pires ◽  
JA Aínsa ◽  
B Gracia ◽  
S Mulhovo ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crude Extracts

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

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