scholarly journals Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Deni Elfiati ◽  
Delvian DELVIAN ◽  
Hamidah Hanum ◽  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat
Keyword(s):  
Peat Soil ◽  
Selective Medium ◽  
Soil Samples ◽  
Available Phosphorus ◽  
Peat Soils ◽  
Insoluble Phosphate ◽  
Value Range ◽  
Qualitative Test ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.

scholarly journals Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

2021 ◽  
Vol 912 (1) ◽  
pp. 012052
Author(s):  
D Elfiati ◽  
Delvian ◽  
A Susilowati ◽  
N W Y Rizki ◽  
A F M Harahap ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
Methyl Cellulose ◽  
Economic Benefits ◽  
Soil Samples ◽  
Morphological Identification ◽  
Rhizophora Stylosa ◽  
Cellulolytic Fungi ◽  
Mangrove Soil ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Mangrove forest is one of the forest ecosystems that can be found in tidal areas. Mangrove forest have ecological, socio-economic benefits for organisms that live in the sea. One of the microorganism that live in mangrove forest is fungus. Therefore, this study aimed to isolate and identify phosphate solubilizing and cellulolytic fungi from mangrove soil. Soil samples were collected from a stands of Rhizophora stylosa in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Soil samples were taken randomly with a dept of 0-20 cm. Isolation of phosphate solubilizing fungi was carried out using Pikovskaya medium and cellulolytic fungi using carboxyl methyl cellulose (CMC) medium. To determine the effectiveness of fungi qualitatively, the phosphate solubility and cellulolytic index were calculated. All isolates obtained were identified morphologically by observing colonies macroscopically and microscopically. This study obtained 12 isolates of phosphate solubilizing and 8 isolates of cellulolytic fungi. Phosphate solubility index ranging from 2.06-2.87 and cellulolytic index of 0.33-3.00. Morphological identifications showed that all isolates of phosphate solubilizing fungi belonged to the genus Aspergillus, while all isolates of cellulolytic fungi belonged to the genus Chaetomium.

scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut

2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Ella Dewani Larasati ◽  
MG Isworo Rukmi ◽  
Endang Kusdiyantini ◽  
R Cinta Badia Ginting
Keyword(s):  
Growth And Development ◽  
Bacillus Pumilus ◽  
Peat Soil ◽  
Species Level ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Peat Soils ◽  
Biolog System ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification

scholarly journals PENGARUH KEDALAMAN MUKA AIR TANAH TERHADAP LILIT BATANG KARET CLON PB260 DAN SIFAT KIMIA TANAH GAMBUT DI KEBUN MERANTI RAPP RIAU

2015 ◽  
Vol 2 (1) ◽  
pp. 5-12
Author(s):  
Aries Sukariawan ◽  
Abdul Rauf ◽  
Arief Setiawan Sutanto ◽  
Bolot Santoso
Keyword(s):  
Water Level ◽  
Water Table ◽  
Peat Soil ◽  
Chemical Properties ◽  
Ash Content ◽  
Soil Samples ◽  
North Sumatra ◽  
Descriptive Method ◽  
Exchangeable P ◽  
And Chemical Properties

Study to evaluate the effect of the depth of water table in peatlands of the PB260 rubber girthimmature plants (age 2-3 years) and chemical properties of peat soil has been carried out inMeranti Estate RAPP Kampar Estuary Pangkalan Kerinci District of Riau in December 2013.The research used a descriptive method with measuring water table depth in selected plots with girthmeasurements of planting rubber trees planted in 2010 and 2011. At two sample plots (representingthe shallowest water level and water level deep) dug each soil profile to the investigation anddecision soil samples. Soil samples were analyzed at the Laboratory of BPTP North Sumatra. Theresults showed that the water table in peatlands deeper, reaching 88.6 cm girth will decrease thegrowth of young rubber trees. Rubber immature plants age 2 years who has the best girth growth ingroundwater at a depth of 29.6 cm deep and 3 years old trunk circumference rubber girth bestgrowth at 65.3 cm water table. The water table is within reach mean 88.6 cm cause fasterdecomposition of peat soil is characterized by C/N ratio of lower and lower quality (fertility) peatsoils with lower ash content, K-exchangeable, Mg-exchangeable, P-available, and P-total.

scholarly journals The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean

2018 ◽  
Vol 10 (3) ◽  
pp. 146-150
Author(s):  
LUFITANUR ALFIAH ◽  
DELITA ZUL ◽  
NELVIA NELVIA
Keyword(s):  
Phosphatase Activity ◽  
Soil Phosphorus ◽  
Peat Soil ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Available P ◽  
Available Phosphorus ◽  
Alkaline Soil ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 μg p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.

Fertility of peat soils in Western Siberia

2020 ◽  
pp. 16-22
Author(s):  
Aleksandr Motorin
Keyword(s):  
Low Power ◽  
Western Siberia ◽  
Nitrate Nitrogen ◽  
Peat Soil ◽  
Average Power ◽  
Perennial Grasses ◽  
Available Phosphorus ◽  
Optimal Range ◽  
Peat Soils ◽  
Gley Soil

The smallest moisture capacity in medium-thick peat soil in a meter layer is 671,2 mm, which is 19,3 % more than that of low-power and 38,9 % peat-gley soil. During the formation of the first mowing of perennial grasses, the moisture reserves in the 0,5‑meter layer were always in the optimal range (0,7–0,9 HB). The lack of moisture during deep occurrence of groundwater (1,2–1,6 m) causes a decrease in the yield of the second mowing of perennial grasses by 46–89 % in peat-gley soil and 25–40 % in medium-power soil. The arable layer of peaty-gley soil contains 4,4 times more nitrates than the average power. Without fertilizing after 3 years, the amount of nitrate nitrogen in peaty-gley soil is reduced by 1,9 times, low- and medium-power soil – by 2,3–2,4 times. The amount of nitrate nitrogen is more than that of ammonia, in peat-gley soil 4 times, low-power – 3,5 times. Reserves of available phosphorus in the arable layer of peaty-gley soil are 2,5 times greater than in lowpower, and 3,1 times in average power. Without fertilizing after 4 years, in the arable layer of peaty-gley soil under perennial grasses, the content of available phosphorus decreased by 45,4 %, in low-power – by 20,5 %, in medium-power – by 15,8 %. The advantage in potassium reserves in the arable layer belongs to peaty-gley soil and is due to its high content in the underlying mineral peat. In peaty-gley soil, the decrease in potassium reserves down the profile is 1,6 times; in thin soil – 3,2 times; average power – 2,5 times. Hay productivity of perennial grasses on peaty-gleyic soil is respectively higher by 8.3 and 24,5 % than on low- and medium-power peaty soils. A crucial role in increasing the yield of herbs and gaining increases is played by the nutritional regime.

scholarly journals Characterization of phosphate solubilizing and cellulolytic fungi isolated from soil under Eurycoma longifolia stands

2021 ◽  
Vol 886 (1) ◽  
pp. 012018
Author(s):  
D Elfiati ◽  
A Susilowati ◽  
T M Siagian
Keyword(s):  
Medicinal Plants ◽  
Economic Value ◽  
Bioactive Compound ◽  
Soil Samples ◽  
Morphological Identification ◽  
Cellulolytic Fungi ◽  
Eurycoma Longifolia ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Eurycoma longifolia is one of the potential medicinal plants and has a high economic value if developed. The bioactive compound produced by E. longifolia has potential as medicine, thus causing increased exploitation of their habitat. This condition resulted in the decreasing population of E .longifolia in nature. The distribution of E. longifolia is influenced by soil fertility conditions. The presence of phosphate solubilizing fungi and cellulolytic fungi in the soil can support the growth of E. longifolia in nature. The purpose of this study was to calculate the population of phosphate solubilizing and cellulolytic fungi and to identify the types of phosphate solubilizing and cellulolytic fungi found in the soil under E. longifolia stands. Soil samples were taken randomly at a depth of 0–20 cm in a natural forest of Papaso Village, Lubu Sutam District, Padang Lawas Regency, North Sumatra. Population and isolation of phosphate solubilizing and cellulolytic fungi were obtained by dilution methods. The isolates obtained were identified morphologically to the genus level by macroscopic and microscopic observation of colonies. The results showed that the population of phosphate solubilizing and cellulolytic fungi were 6.64 × 104 CFU/mL and 9.31 × 103 CFU/mL, respectively. The isolation results in 11 isolates of phosphate solubilizing and 10 isolates of cellulolytic fungi. Based on the morphological identification of phosphate solubilizing fungi isolates consisted of 5 genera, namely Aspergillus, Penicillium, Rhizopus, Fusarium, and Mucor, while isolates of cellulolytic fungi also included 5 genera, namely Aspergillus, Cladosporium, Penicillium, Rhizopus, and Mucor.

scholarly journals THE EFFECT OF WATER TABLE DEEP TO TRUNK CIRCUMFERENCE RUBBER PB260 CLON AND SOIL CHEMISTRY OF PEAT SOIL IN MERANTI ESTATE RAPP OF RIAU

2015 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
Aries Sukariawan ◽  
Abdul Rauf ◽  
Arief Setiawan Sutanto ◽  
Bolot Santoso
Keyword(s):  
Water Level ◽  
Water Table ◽  
Soil Chemistry ◽  
Peat Soil ◽  
Chemical Properties ◽  
Soil Samples ◽  
North Sumatra ◽  
Descriptive Method ◽  
Exchangeable P ◽  
And Chemical Properties

Study to evaluate the effect of the depth of water table in peatlands of the PB260 rubber girthimmature plants (age 2-3 years) and chemical properties of peat soil has been carried out inMeranti Estate RAPP Kampar Estuary Pangkalan Kerinci District of Riau in December 2013.Research used descriptive method with measuring water table depth in selected plots with girthmeasurements of planting rubber trees planted in 2010 and 2011. At two sample plots (representingthe shallowest water level and water level deep) dug each soil profile to the investigation anddecision soil samples. Soil samples were analyzed at the Laboratory of BPTP North Sumatra. Theresults showed that the water table in peatlands deeper, reaching 88.6 cm girth will decrease thegrowth of young rubber trees. Rubber immature plants age 2 years who has the best girth growth inground water at a depth of 29.6 cm deep and 3 years old trunk circumference rubber girth bestgrowth at 65.3 cm water table. Water table is within reach mean 88.6 cm cause fasterdecomposition of peat soil is characterized by C/N ratio of lower and lower quality (fertility) peatsoils with lower ash content, K-exchangeable, Mg-exchangeable, P-available and P-total.

scholarly journals Karakteristik Sifat Fisik Tanah Gambut Ogan Komering Ilir

CANTILEVER ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 79-84
Author(s):  
Siti Muslikah ◽  
Ida Yuliana
Keyword(s):  
Physical Properties ◽  
Forest Fires ◽  
Peat Soil ◽  
Soil Samples ◽  
Peat Soils

Some areas of South Sumatra Province are peatlands scattered in the eastern part, starting from the districts of Musi Rawas, Musi Banyuasin, Ogan Komering Ilir, Muaraenim, and Banyuasin. Around 769,000 hectares of peatland are located in the Ogan Komering Ilir Regency area. Ogan Komering Ilir Regency is an area prone to forest fires. Therefore, it is necessary to conduct research on the characteristics of peat soils in the Ogan Komering Ilir area so that they can be used as consideration in efforts to prevent peatland fires. Sampling was carried out in Kotarayo Village, (Kayuagung District), SP 1 (Pedamaran Timur District), and Tulung Selapan Ilir Village (Tulung Selapan District). Peat soil samples were taken with ASTM D 7015-04 guidelines, and the physical properties of peat soil were tested for samples from the three locations. Based on its physical properties, peat soil samples are classified according to ASTM D4427-84. From the results of the research on peat soils in Kayuagung, Tulung Selapan, and Pedamaran Timur sub-districts, Ogan Komering Ilir Regency, peat soil samples can be classified as sapric-peat soil.

Modes of agricultural use, productivity and fertility of developed lowland peat soils

2020 ◽  
Author(s):  
Aleksandr Glubokovskih
Keyword(s):  
Crop Rotation ◽  
Peat Soil ◽  
Perennial Grasses ◽  
Peat Soils ◽  
Fodder Crop ◽  
Agrochemical Properties ◽  
Agricultural Use ◽  
Fodder Crop Rotation

The results of many years of research on the cultivation of crops in fodder crop rotation on dried peat soil are presented. A productive and agroecological assessment of crop rotation with various saturation with perennial grasses is given. The data on the reduction of peat reserves and changes in the agrochemical properties of the soil are presented.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

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