scholarly journals Hydrolysis of wheat straw hemicelluloses for maximum xylose extraction

2021 ◽  
Vol 939 (1) ◽  
pp. 012006
Author(s):  
Zh Makhatov ◽  
Zh Yelemanova ◽  
R Aitkulova ◽  
Z Narymbayeva ◽  
A Dairabayeva ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Atmospheric Pressure ◽  
Reaction Conditions ◽  
Glucose Yield ◽  
Process Duration ◽  
Mild Conditions ◽  
Dilute Sulfuric Acid ◽  
Hydrolysis Process ◽  
Initial Content ◽  
Hydrolysis Of

Abstract The aim of the study is to select reaction conditions for hydrolysis of wheat straw with dilute sulfuric acid for maximum xylose extraction under mild conditions (at atmospheric pressure and temperature of 100°C). The authors found that maximum glucose yield (72.4-77.1 weight % of the initial content of hemicelluloses in wheat straw) is achieved at a concentration of H2SO4 2-3 weight % and the hydrolysis process duration of 5 hours. Analysis of the obtained hydrolysates showed that they contain cellulose (56.8-70.4 weight %), lignin (19.8-28.8 weight %) and hemicelluloses (2.8-15.3 weight %).

Desulfurization from Gasoline by Polymer Resin at Room Temperature and Atmospheric Pressure

2011 ◽  
Vol 396-398 ◽  
pp. 1283-1286
Author(s):  
Jian Peng Zhu ◽  
Chun Hu Li ◽  
Jia Ling Chen ◽  
Ying Wei Luo
Keyword(s):  
Reaction Time ◽  
Sulfur Content ◽  
Atmospheric Pressure ◽  
Room Temperature ◽  
Oxidative Desulfurization ◽  
Sulfur Compounds ◽  
Molar Ratio ◽  
Reaction Conditions ◽  
Polymer Resin ◽  
Mild Conditions

Abstract. Investigation of polymer resin as catalyst in the oxidative desulfurization (ODS) process has revealed that the method can be applied to make a relative high removal of sulfur compounds. The reaction conditions, including temperature, amount of oxidant and reaction time were studied. The best result occurs under mild conditions with respect to room temperature and atmospheric pressure, to remove 75.54% of the totle sulfur content in the presence of H2O2 with an O/S molar ratio of 17. Possible mechanism is also disscussed.

The development of a process for the hydrolysis of lignocellulosic waste

1987 ◽  
Vol 321 (1561) ◽  
pp. 537-547 ◽  
Keyword(s):  
Wheat Straw ◽  
Inorganic Material ◽  
Second Step ◽  
Lignocellulosic Waste ◽  
Reaction Conditions ◽  
Acid Recovery ◽  
Rapid Dissolution ◽  
The Subject ◽  
Rich Material ◽  
Hydrolysis Of

The degradation of lignocellulose to provide feedstocks for fermentation and chemical use is the subject of increasing worldwide investigation. This paper outlines the current chemical and enzymic methods and discusses the problems presented to the process engineer. In view of these difficulties, the targets for a viable process are described. In the process, wheat straw is used as the feedstock and the removal of the hemicellulose is involved as a first step. In the second step, the majority of the lignin is removed, thereby yielding a cellulose-rich material for conversion to glucose in the final step. The paper concentrates on this last step, which is an acidic hydrolysis carried out in the presence of a metal halide. This combination leads to rapid dissolution of the solid, which is then susceptible to hydrolysis. The reaction conditions, acid recovery and separation of the glucose from inorganic material are described.

Sequential pretreatment strategies under mild conditions for efficient enzymatic hydrolysis of wheat straw

2015 ◽  
Vol 38 (6) ◽  
pp. 1127-1141 ◽  
Author(s):  
Arturo Sanchez ◽  
Juan Carlos Gil ◽  
Oscar A. Rojas-Rejón ◽  
Ana P. de Alba ◽  
Andrea Medina ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Mild Conditions ◽  
Sequential Pretreatment ◽  
Hydrolysis Of

Effects of the Protein in Wheat Straw on Enzymatic Hydrolysis

2013 ◽  
Vol 690-693 ◽  
pp. 1198-1202 ◽  
Author(s):  
Hui Chen ◽  
Lian Jie Wang ◽  
Tao Zhang ◽  
Meng Yu ◽  
Xin Ming Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Degradation Rate ◽  
Cellulose Degradation ◽  
Hydrolysis Time ◽  
Reaction Conditions ◽  
Orthogonal Experiments ◽  
Enzyme Dosage ◽  
Solid Liquid ◽  
Hydrolysis Of

The article used acid protease to remove the protein in wheat straw, and investigated the influence of wheat straw without the protein to enzymatic hydrolysis. By the single factor experiments, the cellulose degradation rate of wheat straw removed the protein was significantly higher than untreated, in the same conditions of enzymatic hydrolysis. It is shortening 24h of enzymatic hydrolysis time and reducing enzyme dosage 90mg/g. By the orthogonal experiments, the optimal reaction conditions for enzymatic hydrolysis of wheat straw with protein removed is the cellulase dosage 170mg, enzymatic time 56h, solid-liquid ratio 1:20, pH 4.8, the reaction temperature 50°C. Compared to the untreated, the cellulose degradation rate increased by 35.58%.

scholarly journals Development of Improved Cellulase Variants for the Conversion of Spent Mushroom Substrate Supplemented with Wheat Straw by Directed Evolution

2021 ◽  
Author(s):  
Valentina Mauriello ◽  
Anna Pennacchio ◽  
Irantzu Alegria Dallo ◽  
Laura Garcia Saez ◽  
Petri Ihalainen ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Wheat Straw ◽  
Recombinant Expression ◽  
Scale Up ◽  
Wild Type ◽  
Spent Mushroom Substrate ◽  
Glucose Yield ◽  
Hydrolysis Of ◽  
Selection Of

Abstract To improve the Spent mushroom substrate (SMS) saccharification, cloning, recombinant expression in Escherichia coli and characterization of two new GH5 family cellulases (Cel1 and Cel2) were performed. Based on enzymes properties, Cel2 was selected for the generation of 30,000 random mutants by directed evolution in order to develop improved biocatalysts. Error-prone Polymerase Chain Reaction was used for diversity generation in cel2 gene and the screening for activity of mutants allowed selection of 63 improved variants that were subjected to a scale up production. Among these, 13 clones exhibited two-fold higher activity than Cel2 and a higher thermoresistance after 72h. The performances of these mutants in the hydrolysis of pretreated SMS/ wheat straw (40/60) were compared to the wild type Cel2 in conjunction with a commercial enzymatic mixture (MetZyme® SUNO™ BOOSTER 144). All the mutants exhibited a glucose yield two-fold or four fold higher than wild-type Cel2 after 72h of incubation.

Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 45 °C

2020 ◽  
Vol 112 ◽  
pp. 85-91
Author(s):  
Monika Marchwicka
Keyword(s):  
Enzymatic Hydrolysis ◽  
Populus Trichocarpa ◽  
Dry Mass ◽  
Glucose Content ◽  
Glucose Yield ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Influence Of Ph ◽  
Hydrolysis Of

Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 45 °C. The enzymatic hydrolysis with the use of industrial enzymes Cellic® CTec2 (Novozymes, Denmark) was carried out within the conditions recommended by the manufacturer and literature. Cellulose obtained by the Kürschner-Hoffer method from a wood of 3-year-old poplar (Populus trichocarpa) was used for the study. Three pH values of 4.8, 5.0 and 5.5 were applied. Also, three amounts of enzymes were used: 25, 50 and 100 mg per 100 mg of the dry mass of cellulose for each pH used. The temperature was 45 °C. Samples were taken after 24 h and subjected to chromatographic analysis to determine the glucose content in the hydrolysates, and then the process parameters allowing for the highest glucose yield after the enzymatic hydrolysis process. The highest glucose yield was obtained for pH 5.0 and 100 mg of enzymes per 100 mg of the dry mass of cellulose – 79 %.

scholarly journals Enzymatic depolymerization of wheat straw polysaccharides

2021 ◽  
Vol 939 (1) ◽  
pp. 012005
Author(s):  
Zh Makhatov ◽  
N Alibayev ◽  
Z Konarbayeva ◽  
B Makhatov ◽  
A Makhatova ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Specific Activity ◽  
Maximum Yield ◽  
High Yield ◽  
Cellulolytic Enzymes ◽  
Cultivation Conditions ◽  
Glucose Yield ◽  
Complex Preparation ◽  
Hydrolysis Of ◽  
Enzymatic Depolymerization

Abstract The purpose of this study is to develop a technology for enzymatic processing for depolymerization of polysaccharides in wheat straw to obtain the maximum yield of glucose and sorbitol. Cellulolytic enzymes endo-1,4-β-glucanase (EC 3.2.1.4) and cellobiose (1,3-β-glucosidase) (CF 3.2.1.21) were isolated and studied in local strains Tr. viride 121, which are grown under deep cultivation conditions. A technology has been developed for obtaining a complex preparation “Cellozyme G20x” with a high yield and specific activity of cellulase, xylanase, β-glucanase and pectinase, and a scheme for purification from cellulases by precipitation, ultrafiltration, and freeze drying is not inferior in efficiency to commercial preparations. The physicochemical properties of the preparation “Cellozyme G20x” have been studied, the optimal parameters of the action and stability of the enzyme preparation have been established. The efficiency of Cellozyme G20x for hydrolysis of straw polysaccharides was 35-40% in terms of glucose yield.

scholarly journals Production of Sugar From Sweet Sorghum Stems with Hydrolysis Method Using Trichoderma viride

2021 ◽  
Vol 13 (1) ◽  
pp. 121-127
Author(s):  
Melycorianda Hubi Ndapamuri ◽  
Maria Marina Herawati ◽  
V Irene Meitiniarti
Keyword(s):  
Trichoderma Viride ◽  
Glucose Production ◽  
Cellulose Content ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Method ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of ◽  
Bagasse Waste

Sorghum stem bagasse waste is one of the materials with high cellulose content. It can be utilized in glucose production through enzymatic hydrolysis of cellulose by Trichoderma viride. This study aims to determine sorghum stem bagasse’s potential in producing glucose, assessing the time and concentration of sorghum stem bagasse in the hydrolysis process to produce glucose optimally and following SNI. Hydrolysis was carried out using a concentration of 5%, 8%, and 11% sorghum stem bagasse for 10, 15, and 20 days. The results showed that sorghum stem bagasse waste could produce glucose with an average glucose yield of 10.09% to 24.40 %. There is a tendency that increasing substrate concentration and hydrolysis time will increase the yield of liquid glucose. The treatment of 5% concentration of sorghum stem bagasse with a long hydrolysis time of 10 days can produce the highest liquid glucose, namely 24.40% with total dissolved solids of 7.40% Brix, the ash content of 0.26%, but 47.54% water content has not met SNI standards. 

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