scholarly journals Beneficial effect of Achromobacter insolitus MB20 and manures in reducing Pythium aphanidermatum disease in cucumber

2021 ◽  
Vol 948 (1) ◽  
pp. 012053
Author(s):  
Yuliar ◽  
N Nuramida ◽  
A Salmah
Keyword(s):  
Soil Structure ◽  
Nutrient Balance ◽  
Soil Nutrient ◽  
Extracellular Enzyme ◽  
Chicken Manure ◽  
Livestock Waste ◽  
Damping Off ◽  
Goat Manure ◽  
Chemical Fungicides

Abstract The increased global livestock production, such as chickens, cows, goats, and rabbits causes increased animal waste in both liquid and solid forms. The utilization of animal manures are considered as a beneficial way to minimize the livestock waste problems. Application of manure is advantageous to the soil nutrient balance, soil structure and moisture-holding capacity, and facilitates environmental protection. A combination of biocontrol agent of Achromobacter insolitus MB20 and manures was studied as alternative to chemical fungicides to protect crop from diseases without damaging the environment and human health. The chemical fungicides cannot be used continously and do not represent a sustainable method control. Result showed that an application of A. insolitus alone reduced cucumber damping-off as high as 60%, and in combination of A. insolitus with chicken manure was 80%, goat manure 70%, cow manure and rabbit urine 65%. The in vitro test indicated that A. insolitus inhibited P. aphanidermatum growth by 28%. This antagonist bacterium also produced extracellular enzyme of protease and cellulase with proteolytic index (PI) value was 1.4 and cellulolytic index (CI) value was 1.7. An application of A. insolitus with chicken manure resulted the best combination and the most effective in reducing of cucumber damping-off.

scholarly journals KAJIAN POTENSI SUMBER ENERGI BIOGAS DARI KOTORAN TERNAK UNTUK BAHAN BAKAR ALTERNATIF DI KECAMATAN KALIREJO KABUPATEN LAMPUNG TENGAH

2014 ◽  
Vol 3 (2) ◽  
Author(s):  
Untung Surya Dharma ◽  
Kemas Ridhuan
Keyword(s):  
Field Data ◽  
Agricultural Waste ◽  
Chicken Manure ◽  
Cow Dung ◽  
Livestock Waste ◽  
Livestock Manure ◽  
Total Potential ◽  
Decomposition Of Organic Matter ◽  
Biogas Energy ◽  
Goat Manure

Biogas is a source of energy that is derived from the decomposition of organic matter by microorganisms in the absence of oxygen (anaerobic) to produce a mixture of several gases are methane (CH 4) and carbon dioxide (CO 2).Biogas can be made from many types of waste material and the rest, a kind of garbage, livestock waste, agricultural waste and others.Things to consider in the development of biogas energy source so that the principle of availability of sustainable biogas as an alternative fuel can be met is a local or regional potential to produce biogas.Communities in the District Central Lampung regency Kalirejo many who maintain livestock.Waste from livestock manure is especially very disturbing and detrimental to health, it is encouraging to capitalize upon the waste as feedstock for biogas production.To assess the potential of biogas energy sources are needed data about the amount of livestock manure every day, in this study is the dung of cows, goats and chickens.Fermentation process for the three types of dirt inside the reactor each trial was conducted to determine the length / time effective and energy biogas produced.From the field data known total number of chickens are farmed as much as 176.264 , goat 12.345and cow 4.488 tail.Manure produced by 1 chicken, 1 goats and one cow in a row that is 0.055 kg / day, 0.98 kg / day and 19.5 kg / day.While the testing laboratory, known Biogas produced from each of 20 kg of dung that chicken manure yield of 2.04 m 3, cow dung yield of 0.56 m 3 and 0.76 m 3 cow dung.From field data and laboratory testing can be calculated local potential in providing sustainable biogas in District Kalirejo Kalirejo Central Lampung regency, namely the potential of biogas from chicken manure at 988.84 m 3 / day, goat manure at 338.75 m 3 / day and cow manure at 3325.61 m 3 / day.So the total potential of biogas that can be generated is equal to 4653.2 m 3 / day.Keywords: Biogas, local potentials, livestock manure, the total potential of biogas.

scholarly journals Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants

2021 ◽  
Vol 7 (3) ◽  
pp. 195
Author(s):  
Amr H. Hashem ◽  
Amer M. Abdelaziz ◽  
Ahmed A. Askar ◽  
Hossam M. Fouda ◽  
Ahmed M. A. Khalil ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Vicia Faba ◽  
Root Rot ◽  
Antifungal Agents ◽  
Damping Off ◽  
Growth Promoters ◽  
Root Rot Disease ◽  
Rot Disease

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.

scholarly journals In vitro conversion of proapoprotein A-I to apoprotein A-I. Partial characterization of an extracellular enzyme activity.

1983 ◽  
Vol 258 (19) ◽  
pp. 11430-11433 ◽  
Author(s):  
C Edelstein ◽  
J I Gordon ◽  
K Toscas ◽  
H F Sims ◽  
A W Strauss ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activity ◽  
Partial Characterization

Combined application of ammonium nitrate and goat manure: Effects on soil nutrients availability, Okra performance and sustainable food security

2021 ◽  
Vol 1 (1) ◽  
pp. 021-028
Author(s):  
Mukhtar Iderawumi ABDULRAHEEM ◽  
Sulaimon Abidemi LAWAL
Keyword(s):  
Block Design ◽  
Chemical Properties ◽  
Soil Nutrient ◽  
Field Trials ◽  
Sub Saharan Africa ◽  
Growth And Yield ◽  
Urea Fertilizer ◽  
Traditional System ◽  
Land Preparation ◽  
Goat Manure

Many see organic agriculture as the most sustainable form of farming and as the paradigm for global food production in the future. One of the solutions to food insecurity and malnutrition in Sub-Saharan Africa is to promote local crops, encourage the use of locally source materials as amendment, improve their traditional system of production, and so diversify subsistence crop. The major reasons are the lack of knowledge and skill in land preparation and agronomic practices, weather uncertainties, pest outbreak and above all the use of fertilizer. Hence, this research will be carried out to investigate the effects of integrated application of Urea fertilizer and Goat Manure on soil Nutrient Availability and Okra performance. Field trials were conducted with four treatments replicated three times in a Randomized Complete Block Design (RCBD). The treatments were Control (no Urea, no goat manure), 8t/ha-1 goat manure + 200kg/ha-1 urea fertilizer; 8t/ha-1 goat manure + 175kg/ha-1 urea fertilizer and 8t/ha-1 goat manure + 150kg/ha-1 urea fertilizer. Treatments were applied three weeks after planting by ring method with Urea and goat manure mixed. Soil physical and chemical properties, growth and yield parameters were evaluated. Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range. 8t/ha-1 goat manure + 200kg/ha-1 urea fertilizer gave the highest plant height, leaf area as well as number of leaves than other treatment. However, the fruits weight, days of 50% flowering, number of fruit, fruits diameter and fruits length were significantly increased at 8t/ha-1 goat manure + 200kg/ha-1 urea fertilizer.

scholarly journals Sensitivity of Rhizoctonia solani AG-2-2 from Sugar Beet to Fungicides

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2427-2433 ◽  
Author(s):  
Sahar Arabiat ◽  
Mohamed F. R. Khan
Keyword(s):  
Sugar Beet ◽  
Rhizoctonia Solani ◽  
Root Rot ◽  
The United States ◽  
Damping Off ◽  
Growth Assay ◽  
The Mean ◽  
Crown And Root Rot ◽  
Mean Concentration

Rhizoctonia damping-off and crown and root rot caused by Rhizoctonia solani are major diseases of sugar beet (Beta vulgaris L.) worldwide, and growers in the United States rely on fungicides for disease management. Sensitivity of R. solani to fungicides was evaluated in vitro using a mycelial radial growth assay and by evaluating disease severity on R. solani AG 2-2 inoculated plants treated with fungicides in the greenhouse. The mean concentration that caused 50% mycelial growth inhibition (EC50) values for baseline isolates (collected before the fungicides were registered for sugar beet) were 49.7, 97.1, 0.3, 0.2, and 0.9 μg ml−1 and for nonbaseline isolates (collected after registration and use of fungicides) were 296.1, 341.7, 0.9, 0.2, and 0.6 μg ml−1 for azoxystrobin, trifloxystrobin, pyraclostrobin, penthiopyrad, and prothioconazole, respectively. The mean EC50 values of azoxystrobin, trifloxystrobin, and pyraclostrobin significantly increased in the nonbaseline isolates compared with baseline isolates, with a resistant factor of 6.0, 3.5, and 3.0, respectively. Frequency of isolates with EC50 values >10 μg ml−1 for azoxystrobin and trifloxystrobin increased from 25% in baseline isolates to 80% in nonbaseline isolates. Although sensitivity of nonbaseline isolates of R. solani to quinone outside inhibitors decreased, these fungicides at labeled rates were still effective at controlling the pathogen under greenhouse conditions.

scholarly journals Diversity of Functional Soil Microbes in Manures and Its Effect on Organic Mustard Green (Brassica juncea) Production

2019 ◽  
Vol 6 (01) ◽  
pp. 60-66
Author(s):  
Ari Kurniawati ◽  
Maya Melati ◽  
Sandra Arifin Aziz ◽  
Purwono Purwono
Keyword(s):  
Crop Production ◽  
Soil Microbes ◽  
Block Design ◽  
Organic Fertilizer ◽  
Research Field ◽  
Chicken Manure ◽  
Long Term Effects ◽  
Soil P ◽  
Goat Manure ◽  
Mustard Green

The application of organic fertilizer, especially manures, for crop production has long-term effects for soil nutrients availability and improvement of soil structure. The improvement of soil properties involves interactions of various types of microorganism in the soil. The research aimed to study the effects of different types of manures on the diversity of functional soil microbes and its effects on organic green mustard production. The experiment was conducted at IPB organic research field, Cikarawang, Darmaga, Bogor, from April to June 2015. The experiment used a randomized complete block design with two factors; the first factor was types of manure, i.e. chicken, cow, and goat manures; the second factor was manure rates, i.e. 0 and 10 ton.ha-1, so there were six treatments in total, replicated three times. The results showed that chicken manure application resulted in a higher mustard green yield and soil-P content than application of goat manure. The addition 10 ton.ha-1 of manures increased C-organic, N, K, and C/N ratio in soil significantly, but mustard green production was not affected. The diversity of microbe population of the soil treated with all types of manures was high. The addition 10 ton.ha-1 of manures decreased the total number of microbes, but increased the number of cellulose-degrading microbes. The population of cellulose-degrading and phosphate-solubilizing microbes in the soil applied with cow manure was higher than those applied with the other manures.

scholarly journals Growth and yield response of local soybean in the giving of various organic fertilizer

2021 ◽  
Vol 905 (1) ◽  
pp. 012028
Author(s):  
M Rahayu ◽  
E Purwanto ◽  
A Setyawati ◽  
A T Sakya ◽  
Samanhudi ◽  
...  
Keyword(s):  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Basic Material ◽  
Yield Response ◽  
Agricultural System ◽  
Cow Manure ◽  
Randomized Design ◽  
Goat Manure

Abstract Soybean is the basic material for any kind of industry, such as tofu and tempeh industries. Soybean cultivation will be better if use a sustainable agricultural system, such as using organic fertilizers. This research purpose was to find out the effect of organic fertilizer on the growth and yield of local soybean. The research used a complete randomized design with one factor which was a variety of organic fertilizers with 6 treatments. Various fertilizers used in this research consist of inorganic fertilizer, liquid organic, compost, cow manure, rabbit urine, chicken manure, and goat manure. Each test unit consists of 4 pots (plants) and each was repeated 4 times. The results showed that the provision of compost and goat manure increased the growth of local soybean plants, include plant height and the number of leaves at 42 DAP and leaf area at 28 DAP. The application of various organic fertilizers (liquid fertilizer, compost, cow manure, goat manure, and rabbit urine) is unable to increase the yield and yield components of local soybeans.

scholarly journals Impact of Biochar Application on Carbon Dynamics and Fertility of Soils Over-fertilization with Compost

2019 ◽  
Author(s):  
Chen-Chi Tsai ◽  
Yu-Fang Chang
Keyword(s):  
Swine Manure ◽  
Carbon Mineralization ◽  
Soil Nutrient ◽  
Principal Component ◽  
C Mineralization ◽  
Soil C ◽  
Fertilized Soil ◽  
Lower Fertility ◽  
The Impact

In Taiwan, farmers often apply excess compost to ensure adequate crop yield in highly frequent tillage, highly weathered, and lower fertility soils. The potential of biochar (BC) for diminishing soil C mineralization, and improving soil nutrient availability in compost over-fertilized soil is promising, but the study is still under-examined. To test the hypothesis, 434 days in vitro C mineralization kinetics of incubation experiment were conducted. Woody BC 0%, 0.5%, 1.0% and 2.0% (w/w) made of lead tree (Leucaena leucocephala (Lam.) de. Wit) were added to an Oxisols, and two Inceptisols of Taiwan. In each treatment, 5% swine manure compost (2 times recommended amount) was added and served as the over-fertilized soil. The results indicated that soil type strongly influenced the impact of BC addition on soil carbon mineralization potential. Respiration per unit of total organic carbon (total mineralization coefficient, TMC) of three studied soils significantly decreased with BC addition increased. Principal component analysis (PCA) suggested that for retaining more plant nutrients in addition to the effects of carbon sequestration, it is recommended that farmer could use locally produced biochars and composts in highly weathered and highly frequent tillage soil. Adding 0.5%-1% woody BC in soil should be reasonable and appropriate.

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