Genome-Wide Analysis of HSP70 Family Protein in Vigna radiata and Coexpression Analysis Under Abiotic and Biotic Stress

Drought stress causes lower crop production globally. Plants have acquired many adaptations to overcome drought stress. Mungbean (Vigna radiata (L.) R.Wilczek) is a legume crop widely cultivated in South, East and Southeast Asia. It is grown in high-temperature areas where drought is the main cause of reduced plant growth and productivity. Plants cope with drought stress by activating different signalling mechanisms. The sucrose non-fermenting-1-related protein kinase 2 family (SnRK2s) is known to play vital roles in osmotic stress and in abscisic acid (ABA) signalling pathways by phosphorylating downstream targets. The genes encoding SnRK2s in mungbean and their detailed characterisation remain unexplored. We have conducted extensive genome-wide analysis for gene prediction, in silico gene analysis, evolutionary analysis and gene-expression profiling under drought-stress conditions by quantitative real-time PCR. Through genome-wide analysis, eight SnRK2 genes were predicted in the mungbean genome and were assigned the names VrSnRK2.1–VrSnRK2.8, according to their order on the chromosomes. The VrSnRK2 genes identified were classified into three clusters based on their phylogenetic relationship with those of Arabidopsis thaliana. Drought stress was imposed on 11-day-old mungbean plants by completely withholding water for 3 days. According to real-time qPCR data, the expression of most of the VrSnRK2 genes was induced by drought stress, indicating their role in the drought-stress response. One of the genes, namely SnRK2.6c, showed highest expression level (12-fold) under drought stress, possibly indicating a critical role under water-deficit conditions. These data provide important information about the VrSnRK2 gene family in mungbean. The results will help in future functional characterisation of VrSnRK2 genes.

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Genome-wide analysis of OSCA gene family members in Vigna radiata and their involvement in the osmotic response

BMC Plant Biology ◽

10.1186/s12870-021-03184-2 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Lili Yin ◽

Meiling Zhang ◽

Ruigang Wu ◽

Xiaoliang Chen ◽

Fei Liu ◽

...

Keyword(s):

Gene Family ◽

Vigna Radiata ◽

Mung Bean ◽

Expression Profiles ◽

Family Members ◽

Regulatory Elements ◽

Evolutionary Analysis ◽

Genome Wide Analysis ◽

Gene Pairs ◽

Genome Wide

Abstract Background Mung bean (Vigna radiata) is a warm-season legume crop and belongs to the papilionoid subfamily of the Fabaceae family. China is the leading producer of mung bean in the world. Mung bean has significant economic and health benefits and is a promising species with broad adaptation ability and high tolerance to environmental stresses. OSCA (hyperosmolality-gated calcium-permeable channel) gene family members play an important role in the modulation of hypertonic stress, such as drought and salinity. However, genome-wide analysis of the OSCA gene family has not been conducted in mung bean. Results We identified a total of 13 OSCA genes in the mung bean genome and named them according to their homology with AtOSCAs. All the OSCAs were phylogenetically split into four clades. Phylogenetic relationship and synteny analyses showed that the VrOSCAs in mung bean and soybean shared a relatively conserved evolutionary history. In addition, three duplicated VrOSCA gene pairs were identified, and the duplicated VrOSCAs gene pairs mainly underwent purifying selection pressure during evolution. Protein domain, motif and transmembrane analyses indicated that most of the VrOSCAs shared similar structures with their homologs. The expression pattern showed that except for VrOSCA2.1, the other 12 VrOSCAs were upregulated under treatment with ABA, PEG and NaCl, among which VrOSCA1.4 showed the largest increased expression levels. The duplicated genes VrOSCA2.1/VrOSCA2.2 showed divergent expression, which might have resulted in functionalization during subsequent evolution. The expression profiles under ABA, PEG and NaCl stress revealed a functional divergence of VrOSCA genes, which agreed with the analysis of cis-acting regulatory elements in the promoter regions of VrOSCA genes. Conclusions Collectively, the study provided a systematic analysis of the VrOSCA gene family in mung bean. Our results establish an important foundation for functional and evolutionary analysis of VrOSCAs and identify genes for further investigation of their ability to confer abiotic stress tolerance in mung bean.

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Genome-wide analysis of the Hsp70 family genes in pepper (Capsicum annuum L.) and functional identification of CaHsp70-2 involvement in heat stress

Plant Science ◽

10.1016/j.plantsci.2016.07.001 ◽

2016 ◽

Vol 252 ◽

pp. 246-256 ◽

Cited By ~ 26

Author(s):

Meng Guo ◽

Jin-Hong Liu ◽

Xiao Ma ◽

Yu-Fei Zhai ◽

Zhen-Hui Gong ◽

...

Keyword(s):

Heat Stress ◽

Capsicum Annuum ◽

Functional Identification ◽

Genome Wide Analysis ◽

Capsicum Annuum L ◽

Hsp70 Family ◽

Genome Wide

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Genome-wide analysis of the soybean CRK-family and transcriptional regulation by biotic stress signals triggering plant immunity

PLoS ONE ◽

10.1371/journal.pone.0207438 ◽

2018 ◽

Vol 13 (11) ◽

pp. e0207438 ◽

Cited By ~ 5

Author(s):

Leonardo Delgado-Cerrone ◽

Alfonso Alvarez ◽

Eilyn Mena ◽

Inés Ponce de León ◽

Marcos Montesano

Keyword(s):

Transcriptional Regulation ◽

Biotic Stress ◽

Plant Immunity ◽

Genome Wide Analysis ◽

Genome Wide ◽

Stress Signals

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Methylglyoxal detoxification by a DJ-1 family protein provides dual abiotic and biotic stress tolerance in transgenic plants

Plant Molecular Biology ◽

10.1007/s11103-017-0613-9 ◽

2017 ◽

Vol 94 (4-5) ◽

pp. 381-397 ◽

Cited By ~ 12

Author(s):

Prasad Melvin ◽

Kondalarao Bankapalli ◽

Patrick D’Silva ◽

P. V. Shivaprasad

Keyword(s):

Transgenic Plants ◽

Stress Tolerance ◽

Biotic Stress ◽

Abiotic And Biotic Stress ◽

Family Protein

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Genome-wide in silico characterization and stress induced expression analysis of BcL-2 associated athanogene (BAG) family in Musa spp.

Scientific Reports ◽

10.1038/s41598-021-04707-5 ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Ashutosh Dash ◽

Siddhesh B. Ghag

Keyword(s):

Cell Death ◽

Expression Analysis ◽

Biotic Stress ◽

In Silico ◽

B Cell Lymphoma ◽

Binding Motif ◽

Abiotic And Biotic Stress ◽

Genome Wide ◽

Differential Expression Pattern ◽

A Genome

AbstractProgrammed cell death (PCD) is a genetically controlled process for the selective removal of damaged cells. Though understanding about plant PCD has improved over years, the mechanisms are yet to be fully deciphered. Among the several molecular players of PCD in plants, B cell lymphoma 2 (Bcl-2)-associated athanogene (BAG) family of co-chaperones are evolutionary conserved and regulate cell death, growth and development. In this study, we performed a genome-wide in silico analysis of the MusaBAG gene family in a globally important fruit crop banana. Thirteen MusaBAG genes were identified, out of which MusaBAG1, 7 and 8 genes were found to have multiple copies. MusaBAG genes were distributed on seven out of 11 chromosomes in banana. Except for one paralog of MusaBAG8 all the other 12 proteins have characteristic BAG domain. MusaBAG1, 2 and 4 have an additional ubiquitin-like domain whereas MusaBAG5-8 have a calmodulin binding motif. Most of the MusaBAG proteins were predicted to be localized in the nucleus and mitochondria or chloroplast. The in silico cis-regulatory element analysis suggested regulation associated with photoperiodic control, abiotic and biotic stress. The phylogenetic analysis revealed 2 major clusters. Digital gene expression analysis and quantitative real-time RT-PCR depicted the differential expression pattern of MusaBAG genes under abiotic and biotic stress conditions. Further studies are warranted to uncover the role of each of these proteins in growth, PCD and stress responses so as to explore them as candidate genes for engineering transgenic banana plants with improved agronomic traits.

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