scholarly journals Isolation and Characterization of Novel Phages Targeting Xanthomonas oryzae: Culprit of Bacterial Leaf Blight Disease in Rice

PHAGE ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 142-151
Author(s):  
Jian Liu ◽  
Suet Lin Chia ◽  
Geok Hun Tan
Keyword(s):  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Isolation And Characterization ◽  
Blight Disease

scholarly journals PENENTUAN PATOTIPE DAN KERAGAMAN GENETIK Xanthomonas oryzae pv. oryzae PADA TANAMAN PADI DI WILAYAH KARESIDENAN BANYUMAS

2011 ◽  
Vol 11 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Heru Adi Djatmiko ◽  
Budi Prakoso ◽  
Nur Prihatiningsih
Keyword(s):  
Genetic Diversity ◽  
Rice Paddy ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Yellow Colour ◽  
Isolation And Characterization ◽  
Progress Curve ◽  
Blight Disease ◽  
Negative Growth

One of the major diseases of rice paddy fields in Indonesia and the Asian countries is bacterial leaf blight or kresek caused by X. oryzae pv. oryzae (Xoo). Losses caused by the disease in Indonesia reached 70–80%, in India reached 74–81%, and Japan reached 20–50%, thus causing great losses in the economy. The objectives of that research were: 1) Characterize Xoo from Karesidenan Banyumas; 2) To study of the amount of damage and AUDPC (the area under disease progress curve) of bacterial leaf blight disease at Karesidenan Banyumas; 3) To obtain of Xoo pathotype by using the test varieties; 4) To obtaining genetic diversity of Xoo that found in Banjarnegara, Purbalingga, Banyumas, Cilacap dan Kebumen region. Research was carried out in several stages: isolation and characterization of Xoo from Barlingmascakeb region, testing of Xoo with five varieties testing, assesment of disease intensity of bacterial leaf blight and AUDPC in the field, and testing the genetic diversity of Xoo. The results showed that pathogen of bacterial blight on rice is Xoo characterized yellow colour of colonies on SPA medium, negative gram reaction, catalase positive, oxidase negative, negative growth at 0.1% TZC, negative starch hydrolisis , and resistance to 0.001% Cu(NO3)2 positive. Xoo pathotype isolats found in Banjarnegara was pathotype X, Cilacap were pathotype I and II and Purbalingga was pathotype II. Eighteen of Xoo from Karesidenan Banyumas (Banjarnegara, Purbalingga, Banyumas, Cilacap and Kebumen) of RAPD differ one from the others.

scholarly journals Mapping bacterial leaf blight disease of rice (Xanthomonas oryzae pv oryzae) in North Sumatra

2020 ◽  
Vol 454 ◽  
pp. 012160
Author(s):  
Safrizal ◽  
Lisnawita ◽  
K Lubis ◽  
F J M Maathuis ◽  
I Safni
Keyword(s):  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Blight Disease ◽  
North Sumatra

scholarly journals KETAHANAN BEBERAPA GENOTIPE PADI TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS ORYZAE PV. ORYZAE)

2016 ◽  
Vol 15 (2) ◽  
pp. 152 ◽  
Author(s):  
Wira Hadianto ◽  
Lukman Hakim ◽  
Bakhtiar .
Keyword(s):  
Block Design ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Bacterial Isolates ◽  
Randomized Complete Block Design ◽  
Resistance Reaction ◽  
Rice Genotypes ◽  
Blight Disease ◽  
Screen House

The resistance rice genotypes against bacterial leaf blight (Xanthomonas oryzae pv. oryzae). The research was conducted to analyze genotypes of rice that were resistant to bacterial leaf blight (BLB) disease. The experiment was conducted at the screen house of Faculty of Agriculture, Syiah Kuala University, Banda Aceh from February to October 2014. This experiment was arranged in randomized complete block design with 73 treatments and 3 replications. IR-BB27 and IR-64 were used as control of resistant and susceptible varieties to BLB, respectively. The result showed that the bacterial isolates tested were virulent. The longest incubation period found in Limboto, Inpari 10, Situ Patenggang, Sirendeh Semantuk Wayla, Tamboen, Sepasie, Bontok and Arias. Genotypes Inpari 1, Limboto, Tuwoti, Inpari 10, Lekat Rambot Linuet, Rom Mokot, Paki Gajah, Tamboen, Bo 100, Sipasie, Bo Minyek, Bontok, Sirendeh Semantuk Wayla and Sambei showed resistance reaction to bacterial leaf blight disease.

scholarly journals CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE

2018 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
Zurai Resti ◽  
Trimurti Habazar ◽  
Deddi Prima Putra ◽  
Nasrun .
Keyword(s):  
Salicylic Acid ◽  
Endophytic Bacteria ◽  
Root Colonization ◽  
Linear Regression Analysis ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacillus Sp ◽  
In Planta ◽  
Blight Disease

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.

scholarly journals Evaluation of Local Black Glutinous Rice Germplasm of Vietnam for Resistance to Bacterial Leaf Blight Disease

2019 ◽  
Vol 1 (3) ◽  
pp. 240-248
Author(s):  
Tung Hoang ◽  
Ton Huu Phan ◽  
Hai Van Tong ◽  
Trung Nam Tran
Keyword(s):  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Resistant Genes ◽  
Glutinous Rice ◽  
Resistant Varieties ◽  
Environmentally Safe ◽  
Susceptible Control ◽  
Blight Disease ◽  
North Vietnam

Most rice growing areas frequently encounter the bacterial leaf blight, Xanthomonas oryzae pv oryzae (Xoo). To prevent the disease, development of resistant varieties is considered to be the most economical and environmentally safe solution. In this study, three PCR-based markers, Npb181, RM122, and P3, were used for the identification of the genes Xa4, xa5, and Xa7, respectively, from 56 local black glutinous rice accessions of Vietnam. Phenotypic screening of the accessions for resistance to 10 Xoo strains of North Vietnam, along with IRBB4, IRBB5, and IRBB7 as resistant controls and IR24 as a susceptible control were carried out in the 2016 Autumn season. 19 accessions containing the resistant genes were found, of these, 6 accessions carried Xa4 gene, 6 accessions carried xa5 gene, and 11 accessions carried Xa7 gene. Three accessions carried two resistance genes, viz. Nep do (Xa4 and Xa7), Pau cam (xa5 and Xa7), and Pe lon cam (Xa4 and xa5). Accessions with xa5 and Xa7 alone or with a combination of two genes (Xa4 and xa5, Xa4 and Xa7, or xa5 and Xa7) were resistantto 8-9 Xoo strains (8-9R/0M/1-2S). Accessions containing Xa4 showed resistance to 5-6 strains of Xoo (5-6R/0M/4-5S). Xoo strain No1 (HUA01043) showed the lowest virulence, infecting only 14 accessions (42R/4M/14S). Strains No3 (HUA 0020131-2), No4 (HUA202361), No5 (HUA20212), and No8 (HUA 020083) showed highest virulence, and they each infected more than 40 accessions with 19R/0M/41S, 20R/0M/40S, 16R/4M/40S, and 20R/0M/40S, respectively. These strains can even infect some accessions containing effective resistant genes (Xa4 or Xa7).

scholarly journals Effect of bioagent application time against bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae (Uyeda and Ishiyama) Dowson

2014 ◽  
Vol 6 (1) ◽  
pp. 95-100
Author(s):  
Gokil Prasad Gangwar ◽  
A. P. Sinha
Keyword(s):  
Disease Severity ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Application Time ◽  
Maximum Increase ◽  
Time Of Application ◽  
Maximum Reduction ◽  
Blight Disease ◽  
Time Sequences

Present investigation was carried out to test the effect of time of application on efficacy of Trichoderma harzianum and Pseudomonas fluorescens formulations against bacterial leaf blight of rice, under field condition. Foliar sprays with bioagent(s) in three time sequences i.e. 7 days before, simultaneously and 7 days after inoculation of Xanthomonas oryzae pv. oryzae inoculation significantly reduced disease severity over check during Kharif 2006 and 2007. Maximum reduction in bacterial leaf blight disease was obtained with the application of T. harzianum on 7 days before inoculation of pathogen during Kharif 2006 and 2007 and maximum increase in grain yield was obtained with the application of PBA-2 seven days before inoculation of pathogen. It was revealed that prophylactic spray of bioagents one week prior to pathogen inoculation was significantly (106 cfu/g) effective in reducing disease severity of bacterial leaf blight of rice as compared to chemical treatment.

scholarly journals Assessment of Bacterial Leaf Blight Disease of Indonesian Rice Germplasms Using SSR Markers

2020 ◽  
Vol 24 (1) ◽  
pp. 35
Author(s):  
Fatimah Fatimah ◽  
Tasliah Tasliah ◽  
Joko Prasetiyono ◽  
Mahrup Mahrup
Keyword(s):  
Ssr Markers ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Losses ◽  
Rice Varieties ◽  
Future Studies ◽  
Rice Plants ◽  
Serious Disease ◽  
Blight Disease

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious disease in rice plants worldwide. Yield losses caused by Xoo can be as high as 50% in some parts of Asia. Xa7 gene can potentially confer a broad resistance to BLB. Evaluation of disease resistance characteristics in early breeding generations of rice is important to develop varieties with better resistance. This study reports the evaluation of 167 Indonesian rice germplasms against three BLB isolates/pathotypes in a green house setting and the genotyping of 56 Indonesian rice germplasm using 12 SSR markers linked to Xa7 BLB resistance gene. The majority of the indigenous rice germplasms was found to be susceptible to three BLB isolates/pathotypes tested. Sate Liko from Bantul, Yogyakarta, Horeg from Cirebon, West Java and Sijem from Malang, East Java revealed consistent resistance to three isolates/pathotypes tested based on BLB evaluation in a greenhouse, UPGMA analysis, and genotyping. Pathotype XII displayed more virulence to Indonesian rice varieties tested compared to pathotypes VI and V. The association analysis using the General linear model identified six markers associated with BLB resistance and two markers were highly associated (RM20589 and RM20590). This information will be useful for future studies of BLB resistance in rice plants.

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