scholarly journals KETAHANAN BEBERAPA GENOTIPE PADI TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS ORYZAE PV. ORYZAE)

2016 ◽  
Vol 15 (2) ◽  
pp. 152 ◽  
Author(s):  
Wira Hadianto ◽  
Lukman Hakim ◽  
Bakhtiar .
Keyword(s):  
Block Design ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Bacterial Isolates ◽  
Randomized Complete Block Design ◽  
Resistance Reaction ◽  
Rice Genotypes ◽  
Blight Disease ◽  
Screen House

The resistance rice genotypes against bacterial leaf blight (Xanthomonas oryzae pv. oryzae). The research was conducted to analyze genotypes of rice that were resistant to bacterial leaf blight (BLB) disease. The experiment was conducted at the screen house of Faculty of Agriculture, Syiah Kuala University, Banda Aceh from February to October 2014. This experiment was arranged in randomized complete block design with 73 treatments and 3 replications. IR-BB27 and IR-64 were used as control of resistant and susceptible varieties to BLB, respectively. The result showed that the bacterial isolates tested were virulent. The longest incubation period found in Limboto, Inpari 10, Situ Patenggang, Sirendeh Semantuk Wayla, Tamboen, Sepasie, Bontok and Arias. Genotypes Inpari 1, Limboto, Tuwoti, Inpari 10, Lekat Rambot Linuet, Rom Mokot, Paki Gajah, Tamboen, Bo 100, Sipasie, Bo Minyek, Bontok, Sirendeh Semantuk Wayla and Sambei showed resistance reaction to bacterial leaf blight disease.

scholarly journals INDUKSI KETAHANAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI PADA TANAMAN PADI DI LAPANGAN MENGGUNAKAN RIZOBAKTERI INDIGENOS

2014 ◽  
Vol 14 (1) ◽  
pp. 57-63
Author(s):  
Andi Khaeruni ◽  
Abdul Rahim ◽  
Syair Syair ◽  
Adriani Adriani
Keyword(s):  
Plant Growth ◽  
Plant Resistance ◽  
Block Design ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Growth And Yield ◽  
Vegetative Plant ◽  
Randomized Complete Block Design ◽  
Blight Disease ◽  
Important Disease

ABSTRACTInduced resistance to bacterial leaf blight disease in rice field by indigenous rhizobacteria.  Bacterial leaf blight is the most important disease on paddy at Southeast Sulawesi. Utilization of biological agents that induce plant resistance is an alternative tool to control bacterial leaf blight disease on paddy. The aim of  the experiment was obtain rhizobacteria that were able to stimulate the growth of paddy plants as well induce plant resistance towards bacterial leaf blight in the field. All experiment units were arranged with a factorial design in a randomized complete block design. The first factor was the rhizobacteria isolates, consisting 4 treatments, i.e: without rhizobacteria (R0), isolate P11a (R1), isolate PKLK5 (IR2), and  mixture P11a and PKLK5 (R3), the second factor is paddy varieties, (V1): IR64 variety, (V2): Cisantana variety. The pathogen was inoculated on leaf when 45 day after crop.  Weekly observation of disease severity, vegetative plant growth (leaf and stem numbers), and yield were conducted. The results showed that the 10 isolates of rhizobacteria tested were able to induce plant resistance toward bacterial leaf blight, stimulated vegetative growth as well as increased yield of paddy plant. Rhizobacteria application could increase the resistance of paddy toward Xanthomonas oryzae pv. oryzae. The application rhizobacteria could increase the vegetative plant growth, application  mixture P11a and PKLK5 isolates showed higher resistance than single application in terms of plant growth and yield, both IR64 and Cisantana varietes

scholarly journals Seleksi Lapang Ketahanan Beberapa Varietas Padi terhadap Infeksi Hawar Daun Bakteri Strain IV dan VIII

2016 ◽  
Vol 17 (2) ◽  
pp. 80 ◽  
Author(s):  
Lina Herlina ◽  
Tiur S. Silitonga
Keyword(s):  
Oryza Sativa ◽  
Block Design ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Rice Varieties ◽  
Resistance Reaction ◽  
Index Of Severity

<p>Field Selection on Several Rice Varieties for Resistance to Bacterial Leaf Blight strain IV and VIII. Bacterial leaf blight (BLB) on rice (Oryza sativa) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the major obstruction for rice production. Powerful strategy to control BLB is generally conducted by planting resistant plant. Meanwhile, the main way to explore germplasm as the source of resistance gene is conducted by selection a wide rice varieties. Field selection for resistance to BLB on 150 rice-varieties (BB-Biogen collection) was conducted in 2009 in Cianjur. Three leaves and flag leaves of individual hills at vegetative and flowering stage were inoculated by clipping the leaf tip with scissors which had been connected with a suspension bottle of bacterial cells (109- 1010 cells/ml) of the isolates which represented bacterial groups (strain) IV and VIII, respectively. Each plant was inoculated with one race. Two weeks after inoculation, length of the lesion developed on the inoculated leaf was measured, as an index of severity of infection by BLB. This experiment was treated as Factorial-Randomized Completed Block Design (two factor-with 3 replications, respectively). The resistancyreaction to strain IV and VIII was observed as the variable tested, while the factors measured consists of plant-varieties and two bacterial strains. The results showed that 11 varieties with a resistance-reaction to Xoo strain IV, inwhich 5 varieties poses a consistent resistance-reaction, i.e: Pulu Bolong, Pelopor, Gombal, Barito and Kapuas. While resistancereaction to Xoo strain VIII obtained only one variety : IR42 (score-1), while 17 others showed intermediate resistancy (score-3).</p><p> </p><p><strong>Abstrak</strong></p><p>Penyakit hawar daun bakteri (HDB) yang disebabkan oleh Xanthomonas oryzae pv. oryzae (Xoo) pada padi (Oryza sativa) merupakan faktor pembatas upaya peningkatan produksi padi. Pencarian gen-gen tahan terhadap HDB sangat diperlukan untuk pengendalian penyakit tersebut. Seleksi ketahanan terhadap HDB dilakukan di Cianjur pada MT 2009 terhadap 150 varietas padi koleksi Bank Gen, BB-Biogen. Isolat Xoo yang diuji adalah strain IV dan VIII. Inokulasi dilakukan dua kali, yaitu pada fase vegetatif dan generatif dengan cara menggunting 3-5 cm dari ujung daun dan daun bendera dengan gunting yang telah dihubungkan dengan botol berisi suspensi Xoo strain IV dan VIII (109-1010 sel/ml). Rancangan percobaan yang digunakan dalam penelitian ini adalah acak kelompok faktorial dengan tiga ulangan. Sebagai varietas pembanding tahan digunakan IRBB7 dan pembanding rentan varietas Kencana Bali. Reaksi ketahanan tertinggi terhadap Xoo strain IV ditunjukkan oleh 11 varietas terpilih dengan reaksi agak tahan, lima varietas di antaranya menunjukkan reaksi yang konsisten agak tahan, yaitu varietas Pulu Bolong, Pelopor, Gombal, Barito, dan Kapuas. Reaksi &amp;lsquo;tahan&amp;rsquo; terhadap Xoo strain VIII ditunjukkan oleh satu varietas introduksi IR42. Untuk varietas terpilih dengan reaksi &amp;lsquo;agak tahan&amp;rsquo; terdapat 17 varietas.</p>

scholarly journals Penggunaan Pestisida Biorasional untuk Pengendalian Penyakit Hawar Daun Bakteri pada Tanaman Padi

2021 ◽  
Vol 2 ◽  
pp. 145-149
Author(s):  
Agus Nurawan ◽  
Yati Haryati ◽  
Kiki Kusyaeri Hamdani
Keyword(s):  
Serratia Marcescens ◽  
Block Design ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacillus Firmus ◽  
West Java ◽  
Rice Plants ◽  
Randomized Complete Block Design ◽  
Blight Disease

Bacterial leaf blight can cause rice loss between 15-80%. Biorational pesticides can be an alternative to controlling the disease. Bacillus firmus, Burkholderia sp, and Serratia marcescens against bacterial leaf blight on rice plants in the field. The study was conducted in Maret-Juni 2014 in the land of the Independent Farmers Group, Cipeuyeum Village, Haurwangi District, Cianjur Regency, West Java. The design uses a randomized complete block design (RCBD) with 6 treatments and 4 replications. The treatments consist of: 1) Mekongga + biorational, 2) Inpari 14 + biorational, 3) Sintanur + biorasional, 4) Mekongga + without biorational, 5) Inpari 14 + without biorational, and 6) Sintanur + without biorational. The results of the study showed that the application of biorational pesticides can reduce the intensity of bacterial leaf blight disease. Sintanur varieties with the application of biorational pesticides produce higher and higher R / C ratios of 6.81 tons ha-1 and 2.79.

scholarly journals Pengujian Daya Hasil dan Ketahanan Penyakit Hawar Daun Bakteri Tanaman Padi Hibrida

2016 ◽  
Vol 44 (2) ◽  
pp. 126
Author(s):  
Ermelinda Maria Lopes Hornai ◽  
Bambang Sapta Purwoko ◽  
Willy Bayuardi Suwarno ◽  
Dan Iswari Saraswati Dewi
Keyword(s):  
Hybrid Rice ◽  
Phenotypic Diversity ◽  
Block Design ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Agronomic Characters ◽  
Male Sterile ◽  
Rice Varieties ◽  
Randomized Complete Block Design ◽  
Blight Disease

ABSTRACT<br /><br />Hybrid rice varieties  is an alternative technology to improve  productivity of low land rice. The results of previous studies have identified and found the male sterile lines Wild Abortive type and Kalinga are resistant to bacterial leaf blight pathotype III, IV and VIII. The objectives of the research were to obtain information on agronomic characters, yield evaluation, genetic parameters, and repeatability information. The experiment was conducted in two locations namely  Muara and Indramayu experimental stations. The design used was a Randomized Complete Block Design with three replication at each site.  Each replicate consisted of 17 hybrid rice and three check varieties. The results from locations showed that genotype BI485A/BP1 (IR53942) has the highest yield of 5.8 ton ha-1. The coefficient of genetic and phenotypic diversity of six agronomic characters was low. The repeatability for seven character observed were low, except for days to flowering. The  scoring value  of bacterial leaf blight disease in Indramayu showed that nine genotypes exhibit resistance.<br /><br />Keywords: cytoplasmic male sterile lines, disease resistance <br /><br />

scholarly journals Evaluation of Yield, Reaction to Diseases, and Grain Physical Attributes of Some Introduced Rice Hybrids in Ghana

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Samuel Oppong Abebrese ◽  
Alex Yeboah ◽  
Wilson Dogbe ◽  
Paul Kofi Ayirebi Dartey ◽  
Richard Akromah ◽  
...  
Keyword(s):  
Grain Yield ◽  
Yield Components ◽  
Hybrid Rice ◽  
Block Design ◽  
Leaf Blight ◽  
Rice Production ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Randomized Complete Block Design ◽  
Physical Attributes

Hybrid rice technology is one of the promising, sustainable, and proven technologies for increasing rice production and productivity with a yield advantage of 15–30% over modern inbred varieties. The potential of hybrid rice has so far not been exploited in Ghana. This study was undertaken to evaluate the yield potential, reaction to diseases, and physical grain attributes of some introduced hybrids. The trials were laid out in a randomized complete block design (RCBD) with three replicates across three locations. Data were taken on grain yield, yield components, reaction to diseases, and grain physical characteristics. Four promising hybrids (SWARNA 2, ARGH 1501, ARGH 1502, and ARGH 1503) with a mean yield advantage of 15–20.8% over the best inbred check “AgraRice” were identified. With few exceptions, the hybrids were broadly adapted and had adequate resistance to blast and bacterial leaf blight. Most of the test hybrids had long slender grains which make them acceptable to the Ghanaian market but lacked aroma.

scholarly journals Ketahanan Berbagai Kultivar Padi Lokal terhadap Penyakit Hawar Daun Bakteri

2016 ◽  
Vol 12 (3) ◽  
pp. 89
Author(s):  
Andi Khaeruni ◽  
Erwin Najamuddin ◽  
Teguh Wijayanto ◽  
Syair Syair
Keyword(s):  
Control Strategies ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Rice Cultivars ◽  
Rice Varieties ◽  
Resistant Varieties ◽  
Rice Disease ◽  
Generative Phase ◽  
Blight Disease ◽  
Screen House

Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae is an important rice disease in Indonesia, including in South East Sulawesi. The use of resistant varieties is one of the effective and environmentally friendly control strategies to suppress the disease. This study aimed to determine the level of resistance of some local rice varieties of South and Southeast Sulawesi against Xanthomonas oryzae pv. oryzae pathotypes IV, VIII and X. The study was conducted in a screen house involving 3 bacterial pathotypes and 11 local rice cultivars. Shearing method was used for inoculation of bacteria to leaf on vegetative and generative phases. Disease incidences were measured 3 weeks after inoculation, and the data was further used to determine the level of resistance of the tested rice cultivars. The results showed that incubation period of the disease was longer on Kelaca cultivar compared to other cultivars. On vegetative phase, this cultivar showed moderate resistant to pathotypes IV and X, and highly resistant to patotype VIII, whereas on the generative phase it showed moderate resistant to pathotypes IV and VII, and highly resistant to pathotype X. Therefore, Kelaca cultivar can be recommended for endemic areas of leaf blight in South and Southeast Sulawesi

scholarly journals Field screening of rice (Oryza sativa L.) genotypes for resistance to bacterial leaf blight (Xanthomonas oryzae pv. oryzae) disease

2021 ◽  
Vol 4 (1) ◽  
pp. 42-49
Author(s):  
Basistha Acharya ◽  
Sujata KC
Keyword(s):  
Oryza Sativa L ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Progress Curve ◽  
Natural Field Condition ◽  
Rice Genotypes ◽  
Different Genetic Background ◽  
Moderately Resistant ◽  
Resistant Genotypes

Rice is the most important crop in Nepal followed by maize and wheat in terms of area (1.4 million ha), production (5.15 million) and productivity (3.5 mt/ha). Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae is considered to be the most widespread and destructive disease of rice in both irrigated and rainfed environments in Nepal. Use of host plant resistance is the most feasible and economical way to combat this disease. However, breakdown of resistance of a variety often occurs after few years of release due to genetic adaptation of the pathogen. Regular screening of the genotypes is utmost for developing the resistant genotypes. Considering the point, this investigation effort has been made to screen the rice genotypes against BLB under natural field condition at Khajura, Banke during 2018 and 2019. In the study, plants were assessed by measuring disease severity (percentage of leaf diseased) and area under the disease progress curve (AUDPC). Differences in resistance among the rice genotypes were observed in both of the tested year. Among the screened 150 rice genotypes during 2018, 6 genotypes scored resistant (R), 81 moderately resistant (MR), 59 moderately susceptible (MS) and 4 susceptible (S) to BLB. Whereas, in 2019, among the tested 315 rice genotypes, none of the genotypes were resistant, 183 MR, 131 MS and 1 S to BLB disease. This showed that the tested genotypes were composed of different genetic background.  

scholarly journals Evaluation of Local Black Glutinous Rice Germplasm of Vietnam for Resistance to Bacterial Leaf Blight Disease

2019 ◽  
Vol 1 (3) ◽  
pp. 240-248
Author(s):  
Tung Hoang ◽  
Ton Huu Phan ◽  
Hai Van Tong ◽  
Trung Nam Tran
Keyword(s):  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Resistant Genes ◽  
Glutinous Rice ◽  
Resistant Varieties ◽  
Environmentally Safe ◽  
Susceptible Control ◽  
Blight Disease ◽  
North Vietnam

Most rice growing areas frequently encounter the bacterial leaf blight, Xanthomonas oryzae pv oryzae (Xoo). To prevent the disease, development of resistant varieties is considered to be the most economical and environmentally safe solution. In this study, three PCR-based markers, Npb181, RM122, and P3, were used for the identification of the genes Xa4, xa5, and Xa7, respectively, from 56 local black glutinous rice accessions of Vietnam. Phenotypic screening of the accessions for resistance to 10 Xoo strains of North Vietnam, along with IRBB4, IRBB5, and IRBB7 as resistant controls and IR24 as a susceptible control were carried out in the 2016 Autumn season. 19 accessions containing the resistant genes were found, of these, 6 accessions carried Xa4 gene, 6 accessions carried xa5 gene, and 11 accessions carried Xa7 gene. Three accessions carried two resistance genes, viz. Nep do (Xa4 and Xa7), Pau cam (xa5 and Xa7), and Pe lon cam (Xa4 and xa5). Accessions with xa5 and Xa7 alone or with a combination of two genes (Xa4 and xa5, Xa4 and Xa7, or xa5 and Xa7) were resistantto 8-9 Xoo strains (8-9R/0M/1-2S). Accessions containing Xa4 showed resistance to 5-6 strains of Xoo (5-6R/0M/4-5S). Xoo strain No1 (HUA01043) showed the lowest virulence, infecting only 14 accessions (42R/4M/14S). Strains No3 (HUA 0020131-2), No4 (HUA202361), No5 (HUA20212), and No8 (HUA 020083) showed highest virulence, and they each infected more than 40 accessions with 19R/0M/41S, 20R/0M/40S, 16R/4M/40S, and 20R/0M/40S, respectively. These strains can even infect some accessions containing effective resistant genes (Xa4 or Xa7).

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