The impact of bisphenol a (BPA) on the placenta

2022 ◽  
Author(s):  
Enoch Appiah Adu-Gyamfi ◽  
Cheryl S Rosenfeld ◽  
Geetu Tuteja
Keyword(s):  
Bisphenol A ◽  
Obstetric Complications ◽  
Migration And Invasion ◽  
Pathological Changes ◽  
Endocrine Disrupting Chemical ◽  
Endocrine Disrupting ◽  
Metabolic Activities ◽  
The Impact ◽  
Common House ◽  
Trophoblast Migration

Abstract Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) that is used in a wide-variety of plastic and common house-hold items. Therefore, there is potential continual exposure to this compound. BPA exposure has been linked to certain placenta-associated obstetric complications such as preeclampsia, fetal growth restriction, miscarriage, and preterm birth. However, how BPA exposure results in these disorders remains uncertain. Hence, we have herein summarized the reported impact of BPA on the morphology and metabolic state of the placenta and have proposed mechanisms by which BPA affects placentation, potentially leading to obstetric complications. Current findings suggest that BPA induces pathological changes in the placenta and disrupts its metabolic activities. Based on exposure concentrations, BPA can elicit apoptotic or anti-apoptotic signals in the trophoblasts; and can exaggerate trophoblast fusion while inhibiting trophoblast migration and invasion to affect pregnancy. Accordingly, the usage of BPA products by pregnant women should be minimized and less harmful alternative chemicals should be explored and employed where possible.

scholarly journals Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study

2021 ◽  
Author(s):  
Aylin Jamali Khaghani ◽  
Parisa Farrokh ◽  
Saeed Zavareh
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Promoter Methylation ◽  
Inhibitory Effect ◽  
Promoter Regions ◽  
Endocrine Disrupting Chemical ◽  
Epigenetic Effects ◽  
Endocrine Disrupting ◽  
The Impact

Background: Bisphenol A (BPA), a synthetic endocrine-disrupting chemical, is a reproductive toxicant. Granulosa cells have significant roles in follicle development, and KIT ligand (KITL) and Anti-Müllerian hormone (AMH) are essential biomolecules produced by them during folliculogenesis. Objective: Due to the widespread use of BPA and its potential epigenetic effects, this study examined the impact of BPA on promoter methylation of amh and kitl genes in mouse granulosa cells. Materials and Methods: Preantral follicles were isolated from ovaries of immature mice and cultured for eight days. Then, follicles were treated with 50 and 100 μM of BPA, and 0.01% (v/v) ethanol for 24 and 72 hr. Growth and degeneration of follicles and antrum formation were analyzed. The granulosa cells were isolated mechanically, and their extracted DNA was treated with sodium bisulfite. The promoter regions of the amh and kitl were analyzed with PCR and sequencing. Results: BPA did not change follicle survival and antrum formation significantly (p = 0.41). However, the culture in the presence of 100 μM BPA had an inhibitory effect on growth. Before BPA treatment, the CpG of the kitl and amh promoters were unmethylated and partially methylated, respectively. While the percent of 5mC in the amh promoter reduced at 100 μM of BPA, it did not alter the kitl promoter methylation. Conclusion: BPA at higher concentrations has an inhibitory effect on follicle growth. Moreover, it seems that the epigenetic impact of BPA restricts to the demethylation of CpG sites. Key words: Bisphenol A, DNA methylation, Granulosa cells.

scholarly journals The role of bisphenol A and its analogues as endocrine disruptors influencing the thyroid gland: a short review

2020 ◽  
Vol 89 (3) ◽  
pp. e441
Author(s):  
Justyna Milczarek-Banach ◽  
Piotr Miśkiewicz
Keyword(s):  
Thyroid Gland ◽  
Bisphenol A ◽  
Metabolic Disorders ◽  
Short Review ◽  
Endocrine Disrupting Chemical ◽  
Endocrine Disrupting ◽  
The Impact ◽  
The Relationship

Bisphenols (BPs) are common plastic additives widely used in industry, hence, human exposure to BPs is inevitable. The best known BP is bisphenol A (BPA), the production of which and its analogues has been increasing worldwide. This chemical is classified as an endocrine-disrupting chemical, inferring with hormonal homeostasis. Indeed, BPA is associated with the development of oestrogen-dependent neoplasms, infertility, metabolic disorders and neurobehavioral disturbances. However, there is a lack of evidence regarding the impact of BPA and its analogues on the thyroid, with most studies mainly performed on animals or in vitro. This review aims to summarise the knowledge regarding the relationship between BPA and its analogues on the thyroid gland.

scholarly journals Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

Micromachines ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 922
Author(s):  
Dong Lee ◽  
Moo-Yeal Lee ◽  
Sukkil Koh ◽  
Mihi Yang
Keyword(s):  
Bisphenol A ◽  
Liver Cells ◽  
Pyrus Pyrifolia ◽  
Endocrine Disrupting Chemical ◽  
Protein Levels ◽  
Ic50 Value ◽  
Endocrine Disrupting ◽  
Cultured Liver Cells ◽  
Ic50 Values ◽  
Hep3b Cells

A micropillar/microwell chip platform with 3D cultured liver cells has been used for HTP screening of hepatotoxicity of bisphenol A (BPA), an endocrine-disrupting chemical. We previously found the hepatotoxicity of BPA is alleviated by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase 2 (ALDH2). In this study, we have tested potential BPA detoxification with Korean pear (Pyrus pyrifolia) extract, stimulators of ADH and ALDH, as well as arbutin, a reference compound in the pears, on the micropillar/microwell chip platform with human liver cells. Surprisingly, the toxicity of BPA was reduced in the presence of Korean pear extract, indicated by significantly increased IC50 values. The IC50 value of BPA with Korean pear extract tested against HepG2 cells was shifted from 151 to 451 μM, whereas those tested against Hep3B cells was shifted from 110 to 204 μM. Among the tested various concentrations, 1.25, 2.5, and 5 mg/mL of the extract significantly reduced BPA toxicity (Ps < 0.05). However, there was no such detoxification effects with arbutin. This result was supported by changes in protein levels of ADH in the liver cells.

Modulation of cytokine expression in human macrophages by endocrine-disrupting chemical Bisphenol-A

2014 ◽  
Vol 451 (4) ◽  
pp. 592-598 ◽  
Author(s):  
Yanzhen Liu ◽  
Chenfang Mei ◽  
Hao Liu ◽  
Hongsheng Wang ◽  
Guoqu Zeng ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cytokine Expression ◽  
Endocrine Disrupting Chemical ◽  
Human Macrophages ◽  
Endocrine Disrupting

scholarly journals Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

2021 ◽  
Vol 22 (21) ◽  
pp. 11808
Author(s):  
Margaux Wieckowski ◽  
Stéphanie Ranga ◽  
Delphine Moison ◽  
Sébastien Messiaen ◽  
Sonia Abdallah ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Courtship Behavior ◽  
Hormone Metabolism ◽  
Environmental Chemical ◽  
Endocrine Disrupting ◽  
Pregnant Mice ◽  
Γ Ray ◽  
Regulatory Decisions ◽  
The Impact ◽  
Steroidogenic Genes

For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the testis. Physical agents, such as ionizing radiation, were also described to perturb spermatogenesis. Despite the fact that we are constantly exposed to numerous environmental chemical and physical compounds, very few studies explore the impact of combined exposure to chemical and physical pollutants on reproductive health. The aim of this study was to describe the impact of fetal co-exposure to BPA and IR on testicular function in mice. We exposed pregnant mice to 10 µM BPA (corresponding to 0.5 mg/kg/day) in drinking water from 10.5 dpc until birth, and we irradiated mice with 0.2 Gy (γ-ray, RAD) at 12.5 days post-conception. Co-exposure to BPA and γ-ray induces DNA damage in fetal germ cells in an additive manner, leading to a long-lasting decrease in germ cell abundance. We also observed significant alteration of adult steroidogenesis by RAD exposure independently of the BPA exposure. This is illustrated by the downregulation of steroidogenic genes and the decrease of the number of adult Leydig cells. As a consequence, courtship behavior is modified, and male ultrasonic vocalizations associated with courtship decreased. In conclusion, this study provides evidence for the importance of broadening the concept of endocrine disruptors to include physical agents, leading to a reevaluation of risk management and regulatory decisions.

scholarly journals The Association between Bisphenol A, Steroid Hormones, and Selected MicroRNAs Levels in Seminal Plasma of Men with Infertility

2021 ◽  
Vol 10 (24) ◽  
pp. 5945
Author(s):  
Ewelina Palak ◽  
Weronika Lebiedzińska ◽  
Sławomir Anisimowicz ◽  
Maria Sztachelska ◽  
Piotr Pierzyński ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Circulating Mirnas ◽  
Endocrine Disrupting Chemical ◽  
Endocrine Disrupting ◽  
Healthy Control ◽  
Mirnas Expression ◽  
Semen Morphology

Bisphenol A (BPA), the most common endocrine-disrupting chemical, has been associated with male reproductive dysfunctions. Recently, it has been shown that BPA may also affect miRNAs expression. Herein, we aimed to evaluate the association of BPA levels with steroid hormone concentration and circulating miRNAs levels to investigate the potential direct effect of BPA on homeostasis in the testis environment. The level of BPA in the seminal plasma of azoospermic men was significantly higher compared to the healthy control. The concentrations of estradiol (E2) and androstenedione (A) were significantly decreased in the seminal plasma of azoospermic men compared to the normospermic men. The levels of miR-let-7a, miR-let-7b, and miR-let-7c were significantly up-regulated, and the level of miR-518f was significantly down-regulated in the seminal plasma of the azoospermic men compared to the healthy control. The level of BPA correlated negatively with sperm concentration and normal semen morphology. A significant positive correlation was found between BPA levels and miR-let-7a and miR-let-7c levels, whereas BPA negatively correlated with miR-518f levels. Our results suggest that BPA may negatively affect sperm quality. Moreover, BPA correlated with the miR-let-7a, miR-let-7c, and miR-518f levels in seminal plasma, which suggests that BPA may act directly in seminal plasma, affecting the testicular environment.

Bisphenol A Exposure Disrupts Enamel Formation via EZH2-Mediated H3K27me3

2021 ◽  
pp. 002203452199579
Author(s):  
H. Li ◽  
D. Cui ◽  
L. Zheng ◽  
Y. Zhou ◽  
L. Gan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Progenitor Cells ◽  
Enamel Formation ◽  
Developmental Defects ◽  
Endocrine Disrupting Chemical ◽  
Complex Biological Process ◽  
Endocrine Disrupting ◽  
Repressive Histone Modification ◽  
Chromatin Immunoprecipitation Sequencing ◽  
Excessive Proliferation

Enamel formation is a serial and complex biological process, during which related genes are expressed progressively in a spatiotemporal manner. This process is vulnerable to environmental cues, resulting in developmental defects of enamel (DDE). However, how environmental factors are biologically integrated during enamel formation is still poorly understood. Here, we investigated the mechanism of DDE elicited by a model endocrine-disrupting chemical, bisphenol A (BPA), in mouse incisors. We show that BPA exposure leads to DDE in mouse incisors, as well as excessive proliferation in dental epithelial stem/progenitor cells. Western blotting, chromatin immunoprecipitation sequencing, and immunofluorescence staining revealed that this effect was accompanied by upregulation of a repressive mark, H3K27me3, in the labial cervical loop of mouse incisors. Perturbation of H3K27me3 methyltransferase EZH2 repressed the level of H3K27me3 and partially attenuated the excessive proliferation in dental epithelial stem/progenitor cells and DDE induced by BPA exposure. Overall, our results demonstrate the essential role of repressive histone modification H3K27me3 in DDE elicited by exposure to an endocrine-disrupting chemical.

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