Placental Tissue mRNA Expression of Angiogenic Factors (AF) and Their Receptors (AFR) in Response to In Vitro Hypoxia as Influenced by Maternal Nutrition in Adolescent Sheep at Day 75 of Pregnancy.

Utero-placental growth and vascular development are critical for pregnancy establishment that may be altered by various factors including assisted reproductive technologies (ART), nutrition, or others, leading to compromised pregnancy. We hypothesized that placental vascularization and expression of angiogenic factors are altered early in pregnancies after transfer of embryos created using selected ART methods. Pregnancies were achieved through natural mating (NAT), or transfer of embryos from NAT (NAT-ET), or IVF orin vitroactivation (IVA). Placental tissues were collected on day 22 of pregnancy. In maternal caruncles (CAR), vascular cell proliferation was less (P<0.05) for IVA than other groups. Compared with NAT, density of blood vessels was less (P<0.05) for IVF and IVA in fetal membranes (FM) and for NAT-ET, IVF, and IVA in CAR. In FM, mRNA expression was decreased (P<0.01–0.08) in NAT-ET, IVF, and IVA compared with NAT for vascular endothelial growth factor (VEGF) and its receptorFLT1, placental growth factor (PGF), neuropilin 1 (NP1) andNP2, angiopoietin 1 (ANGPT1) andANGPT2, endothelial nitric oxide synthase 3 (NOS3), hypoxia-inducible factor 1A (HIF1A), fibroblast growth factor 2 (FGF2), and its receptorFGFR2. In CAR, mRNA expression was decreased (P<0.01–0.05) in NAT-ET, IVF, and IVA compared with NAT forVEGF,FLT1,PGF,ANGPT1, andTEK. Decreased mRNA expression for 12 of 14 angiogenic factors across FM and CAR in NAT-ET, IVF, and IVA pregnancies was associated with reduced placental vascular development, which would lead to poor placental function and compromised fetal and placental growth and development.

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Impacts of maternal nutrition on uterine and placental vascularity and mRNA expression of angiogenic factors during the establishment of pregnancy in beef heifers1

Translational Animal Science ◽

10.2527/tas2017.0019 ◽

2017 ◽

Vol 1 (2) ◽

pp. 160-167 ◽

Cited By ~ 5

Author(s):

K. J. McLean ◽

M. S. Crouse ◽

M. R. Crosswhite ◽

N. Negrin Pereira ◽

C. R. Dahlen ◽

...

Keyword(s):

Gene Expression ◽

Mrna Expression ◽

Maternal Nutrition ◽

Control Diet ◽

Uterine Horn ◽

Angiogenic Factors ◽

Angiogenic Factor ◽

Nutrient Restriction ◽

Beef Heifers ◽

Vascular Volume

Abstract We hypothesized that maternal nutrient restriction starting at the time of breeding would influence placental vascular development and gene expression of angiogenic factors during the first 50 d of gestation in beef heifers. Commercial Angus crossbred heifers (n = 49) were maintained on a total mixed ration and supplemented with dried distillers grains with solubles. All heifers were subject to 5-d CO-Synch + CIDR estrous synchronization protocol, AI to a single Angus sire, and randomly assigned to dietary treatments. One half were assigned to control diet (CON) targeted to gain 0.45 kg/d and the remaining half were assigned to restricted diet (RES), which received 60% of CON. Heifers were subjected to ovariohysterectomy on d 16, 34, or 50 of gestation. Utero-placental tissues were obtained from the uterine horns ipsilateral and contralateral to the corpus luteum and separated into maternal caruncle (CAR); maternal endometrium, inter-caruncle (ICAR), and fetal membranes (FM). After collection, all tissues were snap frozen and stored at –80°C. There were no treatment × stage of gestation interactions (P >0.13) on the mRNA expression of vascular endothelial growth factor (VEGF) or endothelial nitric oxide synthase (eNOS). Heifers on CON treatment had greater (P = 0.03) expression of VEGF compared with RES heifers in NP-ICAR. On d 50 expression of eNOS was increased (P = 0.05) compared with d 16 in P-CAR. Expression of eNOS mRNA was decreased (P = 0.04) on d 16 compared with d 34 and 50 in CON heifer. Gene expression of eNOS was increased (P < 0.001) in the pregnant uterine horn compared with the NP uterine horn on d 34 and 50. Expression of eNOS was also increased (P < 0.003) on d 34 and 50 in the pregnant uterine horn compared with FM. There was a maternal nutritional plane × stage of gestation interaction (P = 0.01) on the vascular ratio (vascular volume/tissue volume) in maternal tissues. The RES heifers had a greater vascular ratio on d 16 compared with d 34 and 50; whereas, CON heifers had a greater vascular ratio on d 34 compared with d 16 and 50. In the NP uterine horn, there was also an increase (P = 0.02) in vascular volume of FM from CON heifers compared with FM from RES heifers. We conclude that maternal nutrient restriction did alter both vascularity and mRNA expression of angiogenic factor in utero-placental tissues during the establishment of pregnancy in first parity beef heifers.

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RBMS1 Methylation and mRNA Expression Are Differentially Regulated in Placenta Tissue from Obese Women (P11-131-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz048.p11-131-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Cited By ~ 1

Author(s):

Travis Alvine ◽

Archana Dhasarathy ◽

Amy Bundy ◽

Atrayee Bhattacharya ◽

Diane Darland ◽

...

Keyword(s):

Dna Methylation ◽

Mrna Expression ◽

Rna Binding ◽

Protein Localization ◽

Placental Tissue ◽

Obese Women ◽

Placental Inflammation ◽

Anti Inflammatory ◽

Western Division

Abstract Objectives The maternal diet and obesity adversely affect placental structure/function, inflammation, and alters placental DNA methylation. RNA-binding motif single-stranded interacting protein 1 (RBMS1) regulates DNA replication and gene transcription and is expressed in the placenta. Although specific roles for RBMS1 in the context of obesity have not yet been fully explored, RBMS1 has been linked to type 2 diabetes mellitus risk. No data are available regarding the function of RBMS1 in placental tissue and whether placental expression of RBMS1 is regulated differentially in obesity. Methods Placental tissue from obese (BMI > 25, n = 15) and non-overweight (BMI < 25, n = 3) women was obtained from The Cooperative Human Tissue Network (CHTN) Western Division at Vanderbilt University Medical Center. Epigenetic changes in placental RBMS1 DNA methylation and histone acetylation were assessed by DNA methylation assays and chromatin immunoprecipitation, respectively. Placental RBMS1 gene expression was measured by RT-PCR and RBMS1 protein localization was assessed by immunohistochemistry. The anti-inflammatory effect of RBMS1 was measured in vitro using human placental fibroblast cells (HS 795.PI). Placental short-chain fatty acids (SCFAs) were measured by gas chromatography-mass spectrometry (GC-MS). Results RBMS1 mRNA (P = 0.0029) and DNA methylation (P < 0.05) were increased in placentae from obese mothers. RBMS1 protein expression was localized to placental resident macrophages and fibroblastic cells. In vitro, RBMS1 recombinant protein attenuated LPS-induced IL-6 mRNA expression in HS 795.PI cells (P < 0.001), suggesting a possible anti-inflammatory role for RBMS1 in the context of placental inflammation. SCFA analysis demonstrated an increase in placental butyrate (P = 0.0327), a known histone deacetylase (HDAC) inhibitor in placenta from obese women. Conclusions These data suggest a possible anti-inflammatory role for RBMS1 in the context of placental inflammation. Increased placental RBMS1 expression in obese women may serve as an adaptive response to reduce placental inflammation. Funding Sources USDA Agricultural Research Service Project #3062-51000-052-00D.

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Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or placental tissue promoted HUVECs angiogenesis in vitro

10.21203/rs.3.rs-54126/v1 ◽

2020 ◽

Author(s):

Haiying Ma ◽

Shenglu Jiang ◽

Lili Du ◽

Jinfang Liu ◽

Xiaoyan Xu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Early Pregnancy ◽

Placental Tissue ◽

Angiogenic Factors ◽

Antibody Array ◽

Cell Type ◽

Placental Angiogenesis ◽

Placental Cells

Abstract Background As a large capillary network, the human placenta plays an important role throughout pregnancy. Placental vascular development is complex and delicate and involves many types of placental cells, such as trophoblasts, and mesenchymal stem cells. There has been no systematic, comparative study on the roles of these two groups of placental cells and the whole placental tissue in the placental angiogenesis. In this study, primary cytotrophoblasts (CTBs) from early-pregnancy and primary human placenta-derived mesenchymal stem cells (hPDMSCs) from different stages of pregnancy were selected as the cell research objects, and full-term placental tissue was selected as the tissue research object to detect the effects of their conditioned medium (CM) on HUVECs angiogenesis.Methods We successfully isolated primary hPDMSCs and CTBs, collected CM from these placental cells and placental tissue, and then evaluated the effects of the CM on a series of angiogenic processes in HUVECs in vitro. Furthermore, we measured the levels of angiogenic factors in the CM of placental cells or tissue by an angiogenesis antibody array.Results The results showed that not only placental cells but also placental tissue, to some extent, promoted HUVECs angiogenesis in vitro by promoting proliferation, adhesion, migration, invasion, and tube formation. We also found that primary placental cells in early pregnancy, whether CTBs or hPDMSCs, played more significant roles than those in middle and full-term pregnancy. The effect of CM from placental tissue was better than that of CM from a single placental cell type. The semiquantitative angiogenesis antibody array showed that, in placental tissue-derived CM, 18 of the 43 angiogenic factors had obvious spots, and the levels of 5 factors (including CXCL-5, GRO, IL-6, IL-8, and MCP-1) were the highest. The levels of these 18 angiogenic factors in placental tissue were higher than those in any single placental cell type.Conclusions CM obtained from placental cells (primary CTBs or hPDMSCs) or placental tissue contained proangiogenic factors and promoted HUVECs angiogenesis in vitro. Therefore, our research is helpful to better understand placental angiogenesis regulation and provides theoretical support for the clinical application of placental components, especially placental tissue-derived CM, in vascular tissue engineering and clinical treatments.

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Evidence for episodic GnRH-modulated hCG secretion by placental tissue in vitro

Acta Endocrinologica ◽

10.1530/acta.0.120s103 ◽

1989 ◽

Vol 120 (3_Suppl) ◽

pp. S103 ◽

Cited By ~ 1

Author(s):

W. E. MERZ ◽

C. ERLEWEIN ◽

P. LICHT ◽

T. O. F. WAGNER

Keyword(s):

Placental Tissue ◽

Hcg Secretion

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The truncated somatostatin receptor sst5TMD4 stimulates the production of pro-angiogenic factors in in vitro and in vivo breast cancer models

Endocrine Abstracts ◽

10.1530/endoabs.35.p516 ◽

2014 ◽

Author(s):

Raul M Luque ◽

Mario Duran-Prado ◽

David Rincon-Fernandez ◽

Marta Hergueta-Redondo ◽

Michael D Culler ◽

...

Keyword(s):

Breast Cancer ◽

Somatostatin Receptor ◽

Angiogenic Factors ◽

Cancer Models

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Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

Current Drug Delivery ◽

10.2174/1567201818666201214125237 ◽

2020 ◽

Vol 18 ◽

Author(s):

Zirui Zhang ◽

Shangcong Han ◽

Panpan Liu ◽

Xu Yang ◽

Jing Han ◽

...

Keyword(s):

Wound Healing ◽

Mrna Expression ◽

Tissue Injury ◽

Inflammatory Cells ◽

Pcr Analysis ◽

Protective Effects ◽

Deep Tissue ◽

Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

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A Pro-Somathropic Formulation with GHR Agonistic and Pro-Thyrogenic Features (Preprint)

10.2196/preprints.15768 ◽

2019 ◽

Author(s):

Erdal Can Alkoclar

Keyword(s):

Mrna Expression ◽

Efficient Method ◽

Age Related

BACKGROUND A Formulation consisting of 2 Dioscin and 2 Glucopyranoside Derivatives with Simultaneous GHRH Stimulative and T3 mRNA Expression Enhancimg Features. OBJECTIVE Anti Aging METHODS GH/T3 Optimization RESULTS Approved in Vitro CONCLUSIONS Endogenous GH and T3 Optimization is the an efficient method for combating age related Senility and Fatigue Symptoms

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Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22094604 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4604

Author(s):

Giuliana Mannino ◽

Anna Longo ◽

Florinda Gennuso ◽

Carmelina Daniela Anfuso ◽

Gabriella Lupo ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Mrna Expression ◽

Inflammatory Cytokines ◽

High Glucose ◽

Angiogenic Factors ◽

Diabetic Patients ◽

Ros Production ◽

Migration Ability ◽

And Migration

A pericyte-like differentiation of human adipose-derived mesenchymal stem cells (ASCs) was tested in in vitro experiments for possible therapeutic applications in cases of diabetic retinopathy (DR) to replace irreversibly lost pericytes. For this purpose, pericyte-like ASCs were obtained after their growth in a specific pericyte medium. They were then cultured in high glucose conditions to mimic the altered microenvironment of a diabetic eye. Several parameters were monitored, especially those particularly affected by disease progression: cell proliferation, viability and migration ability; reactive oxygen species (ROS) production; inflammation-related cytokines and angiogenic factors. Overall, encouraging results were obtained. In fact, even after glucose addition, ASCs pre-cultured in the pericyte medium (pmASCs) showed high proliferation rate, viability and migration ability. A considerable increase in mRNA expression levels of the anti-inflammatory cytokines transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) was observed, associated with reduction in ROS production, and mRNA expression of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and angiogenic factors. Finally, a pmASC-induced better organization of tube-like formation by retinal endothelial cells was observed in three-dimensional co-culture. The pericyte-like ASCs obtained in these experiments represent a valuable tool for the treatment of retinal damages occurring in diabetic patients.

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