Microtitration of Free Fatty Acids in Plasma

1967 ◽  
Vol 13 (1) ◽  
pp. 36-39 ◽  
Author(s):  
J E Goss ◽  
Allen Lein
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Extraction Procedure ◽  
Phase System

Abstract A method for determination of free fatty acids in plasma has been developed which employs a modification of Dole's extraction procedure and involves a 1-phase system for titration rather than the 2-phase system most commonly used.

Enzymic and chemical-extraction determinations of free fatty acids in serum compared.

1982 ◽  
Vol 28 (8) ◽  
pp. 1765-1768 ◽  
Author(s):  
P N Demacker ◽  
A G Hijmans ◽  
A P Jansen
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Extraction Procedure ◽  
Short Chain Fatty Acids ◽  
Chemical Extraction ◽  
Keto Acids ◽  
Test Kit ◽  
Hydrolysis Of ◽  
Chain Fatty Acids

Abstract We compared an enzymic test kit for determination of free fatty acids in serum (NEFA C-test, WAKO) with two modifications of a chemical extraction procedure, I (Clin. Chim. Acta 43: 317-320, 1973) and II (Clin. Chim. Acta 80: 327-332, 1977). All three procedures are specific for long- and medium-chain fatty acids. Short-chain fatty acids, some keto acids, and phospholipids did not interfere. Added fatty acid was quantitatively accounted for in all methods. Results obtained with the enzymic method and II did not differ significantly, whereas the results by I were about 10% lower. The concentration of NaCl in the copper reagent, but not the kind of solvent used to dissolve the standard, influenced the accuracy of the chemical methods. In the enzymic procedure, hydrolysis of triglycerides during incubation is unlikely to be the reason for too-high values. The precision of all three procedures is acceptable for use in clinical laboratories.

Automated Colorimetric Determination of Free Fatty Acids in Biologic Fluids

1967 ◽  
Vol 13 (9) ◽  
pp. 744-751 ◽  
Author(s):  
C Dalton ◽  
C Kowalski
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method ◽  
Extraction Procedure ◽  
Automated Analysis ◽  
Colorimetric Determination ◽  
Direct Extraction

Abstract For the routine determination of free fatty acids (FFA) the authors recommend a modification of the automated colorimetric method of Antonis (1) using the extraction procedure of ltaya and Ui (2). This direct extraction and automated analysis shows good correlation with Trout's (3) modification of Dole's (4) titrimetric procedure.

scholarly journals Determination of Free Fatty Acids in Sausage Meat

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Codrean POP ◽  
Levente BARA ◽  
Elena HORJ ◽  
Andreea IORDACHE ◽  
Cornel LASLO ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Mass Spectra ◽  
Methyl Esters ◽  
Extraction Procedure ◽  
Internal Standard ◽  
Quadrupole Mass ◽  
Temperature Program ◽  
Separation Temperature

The aim of this work was to establish the extraction procedure, the derivatization method, the separation temperature program, the identification and quantitation of the free fatty acids from meat. A gas chromatography - mass spectrometric (GC/MS) technique was used. The fatty acids were obtained by grounding meat, water and sand and then extraction was performed by mixing chloroform:methanol (2:1) during 30 seconds, at room temperature. The fatty acids were derivatized to obtain methyl esters. A Trace DSQ ThermoFinnigan quadrupole mass spectrometer coupled with a Trace GC was used. Fatty acids were separated on a Rtx-5MS capillary column, 30m x 0.25mm, 0.25µm film thickness, using a suitable temperature program. The identification of fatty acids was obtained by comparison of fatty acids methyl esters (FAME) mass spectra with the mass spectra of FAME kits and of NIST library. Concentrations of fatty acids were calculated by using a proper internal standard.

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