scholarly journals Determination of Free Fatty Acids in Sausage Meat

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Codrean POP ◽  
Levente BARA ◽  
Elena HORJ ◽  
Andreea IORDACHE ◽  
Cornel LASLO ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Mass Spectra ◽  
Methyl Esters ◽  
Extraction Procedure ◽  
Internal Standard ◽  
Quadrupole Mass ◽  
Temperature Program ◽  
Separation Temperature

The aim of this work was to establish the extraction procedure, the derivatization method, the separation temperature program, the identification and quantitation of the free fatty acids from meat. A gas chromatography - mass spectrometric (GC/MS) technique was used. The fatty acids were obtained by grounding meat, water and sand and then extraction was performed by mixing chloroform:methanol (2:1) during 30 seconds, at room temperature. The fatty acids were derivatized to obtain methyl esters. A Trace DSQ ThermoFinnigan quadrupole mass spectrometer coupled with a Trace GC was used. Fatty acids were separated on a Rtx-5MS capillary column, 30m x 0.25mm, 0.25µm film thickness, using a suitable temperature program. The identification of fatty acids was obtained by comparison of fatty acids methyl esters (FAME) mass spectra with the mass spectra of FAME kits and of NIST library. Concentrations of fatty acids were calculated by using a proper internal standard.

Liquid Chromatographic Method for Quantitative Determination of Free Fatty Acids in Butter

1984 ◽  
Vol 67 (4) ◽  
pp. 718-721 ◽  
Author(s):  
Alan W Reed ◽  
Hilton C Deeth ◽  
Donald E Clegg
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Chromatographic Method ◽  
Water System ◽  
Internal Standard ◽  
Detection Range ◽  
Water Solvent ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method has been developed for the determination of free fatty acids in butter. The fatty acids are converted to the p-bromophenacyl esters, via a crown ether-catalyzed reaction, without separation from the other butter components. The esters are separated on a Cis-bonded silica column by using an acetonitrile-water solvent gradient and quantitated using the ester of heptadecanoic acid as internal standard. Cu, and C ,S:i co-elute in the acetonitrile-water system but are separated using an isocratic methanol-acetonitrile-water system. Limits of detection range from 7 ng for butyric acid to 45 ng for linoleic acid. The average coefficient of variation (n = 10) for 10 free fatty acids from,a butter was 5.83%.

Enzymic and chemical-extraction determinations of free fatty acids in serum compared.

1982 ◽  
Vol 28 (8) ◽  
pp. 1765-1768 ◽  
Author(s):  
P N Demacker ◽  
A G Hijmans ◽  
A P Jansen
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Extraction Procedure ◽  
Short Chain Fatty Acids ◽  
Chemical Extraction ◽  
Keto Acids ◽  
Test Kit ◽  
Hydrolysis Of ◽  
Chain Fatty Acids

Abstract We compared an enzymic test kit for determination of free fatty acids in serum (NEFA C-test, WAKO) with two modifications of a chemical extraction procedure, I (Clin. Chim. Acta 43: 317-320, 1973) and II (Clin. Chim. Acta 80: 327-332, 1977). All three procedures are specific for long- and medium-chain fatty acids. Short-chain fatty acids, some keto acids, and phospholipids did not interfere. Added fatty acid was quantitatively accounted for in all methods. Results obtained with the enzymic method and II did not differ significantly, whereas the results by I were about 10% lower. The concentration of NaCl in the copper reagent, but not the kind of solvent used to dissolve the standard, influenced the accuracy of the chemical methods. In the enzymic procedure, hydrolysis of triglycerides during incubation is unlikely to be the reason for too-high values. The precision of all three procedures is acceptable for use in clinical laboratories.

Automated Colorimetric Determination of Free Fatty Acids in Biologic Fluids

1967 ◽  
Vol 13 (9) ◽  
pp. 744-751 ◽  
Author(s):  
C Dalton ◽  
C Kowalski
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method ◽  
Extraction Procedure ◽  
Automated Analysis ◽  
Colorimetric Determination ◽  
Direct Extraction

Abstract For the routine determination of free fatty acids (FFA) the authors recommend a modification of the automated colorimetric method of Antonis (1) using the extraction procedure of ltaya and Ui (2). This direct extraction and automated analysis shows good correlation with Trout's (3) modification of Dole's (4) titrimetric procedure.

Microtitration of Free Fatty Acids in Plasma

1967 ◽  
Vol 13 (1) ◽  
pp. 36-39 ◽  
Author(s):  
J E Goss ◽  
Allen Lein
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Extraction Procedure ◽  
Phase System

Abstract A method for determination of free fatty acids in plasma has been developed which employs a modification of Dole's extraction procedure and involves a 1-phase system for titration rather than the 2-phase system most commonly used.

scholarly journals Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 479
Author(s):  
Shihan Wang ◽  
Yuanshuai Gan ◽  
Hong Kan ◽  
Xinxin Mao ◽  
Yongsheng Wang
Keyword(s):  
Fatty Acids ◽  
Quantitative Analysis ◽  
Unsaturated Fatty Acids ◽  
Internal Standard ◽  
Active Components ◽  
Reliable Determination ◽  
External Standard Method ◽  
Single Marker ◽  
Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

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