Micromethod for Total Serum Cholesterol that Eliminates Interference by High Bilirubin Concentrations

1970 ◽  
Vol 16 (1) ◽  
pp. 18-19 ◽  
Author(s):  
William J Jordan ◽  
Edward C Knoblock
Keyword(s):  
Total Cholesterol ◽  
Serum Lipids ◽  
Room Temperature ◽  
Dextran Sulfate ◽  
Direct Method ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Lipoprotein Complex ◽  
Acid Reagent ◽  
High Bilirubin

Abstract The analysis of pediatric serum for total cholesterol in the presence of high amounts of bilirubin presents special problems. The serum specimens are so small that a direct micro- or ultramicromethod has to be used, and consequently the error owing to bilirubin is impressive. In the proposed method, cholesterol is precipitated, along with other serum lipids, from only 20 µliters of serum as a dextran sulfate—lipoprotein complex. The precipitate is dissolved in molar NaCl, treated with glacial acetic acid, and incubated for 5 min at 70°C. A ferric chloride—sulfuric acid reagent is then added and, after 20 min at room temperature, the color is read at 560 nm. Total cholesterol values obtained by the proposed method are not statistically different from those obtained by the direct method of Pearson et al. Bilirubin added to sera does not affect the results. Agreement was obtained with the stated cholesterol values for Versatol-A and Moni-trol II reference sera to which known amounts of cholesterol and bilirubin had been added by the manufacturer.

A Modified Total Serum Cholesterol Method to Eliminate the Effect of High Bilirubin Levels

1968 ◽  
Vol 14 (1) ◽  
pp. 31-34 ◽  
Author(s):  
William J Jordan
Keyword(s):  
Serum Cholesterol ◽  
Dextran Sulfate ◽  
Serum Bilirubin ◽  
Sulfate Solution ◽  
High Serum ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Lipoprotein Complex ◽  
High Bilirubin ◽  
High Serum Bilirubin

Abstract When a high molecular weight dextran sulfate solution was added to serum in the presence of calcium ions, a lipoprotein complex was precipitated that contained the total serum cholesterol. The incorporation of this precipitation step in the procedure for total cholesterol determinations eliminated errors due to high serum bilirubin levels.

Total serum cholesterol by isotope dilution/mass spectrometry: a candidate definitive method.

1980 ◽  
Vol 26 (7) ◽  
pp. 854-860 ◽  
Author(s):  
A Cohen ◽  
H S Hertz ◽  
J Mandel ◽  
R C Paule ◽  
R Schaffer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Total Cholesterol ◽  
Serum Cholesterol ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Weight Ratio ◽  
Molecular Ions ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Definitive Method

Abstract We describe a highly accurate and precise method for determination of total cholesterol in serum by isotope dilution/mass spectrometry. The method was developed for a Study Group of the Committee on Standards of the American Association for Clinical Chemistry, for use in establishing the accuracy of a candidate reference method for total cholesterol, and fulfills their criteria for a definitive method. Cholesterol-d7 is added to serum, with the weight ratio of cholesterol-d7 to total serum cholesterol kept near to 1:1. The esters are hydrolyzed and the cholesterol is separated and converted into the trimethylsilyl ether derivative for measurement by combined gas chromatography/mass spectrometry. The intensity ratio of the molecular ions at m/z 465 and 458 is measured for each sample and for two calibration mixtures, according to a prescribed bracketing protocol. A weight ratio for the sample is obtained by linear interpolation of the ion-intensity ratios, and the total cholesterol is then calculated. The method was applied four times over several weeks to each of five serum pools. Statistical analysis involving consideration of both replication error and variability between weeks gave a coefficient of variation for a single measurement of 0.36%. The absence of interferences in the method was demonstrated by measurements at several other masses.

scholarly journals The effect of coffee consumption on serum total cholesterol in the Sami and Norwegian populations

2010 ◽  
Vol 13 (11) ◽  
pp. 1818-1825 ◽  
Author(s):  
Tove Nystad ◽  
Marita Melhus ◽  
Magritt Brustad ◽  
Eiliv Lund
Keyword(s):  
Total Cholesterol ◽  
Serum Cholesterol ◽  
Coffee Consumption ◽  
Cross Sectional Study ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Cross Sectional ◽  
Cholesterol Levels ◽  
The Mean ◽  
The Impact

AbstractObjectiveTo assess coffee consumption in the Sami and Norwegian populations and to investigate the impact of unfiltered boiled coffee consumption on serum cholesterol concentrations.DesignA cross-sectional study. Information was collected by self-administrated questionnaires and total serum cholesterol was analysed. Participants were divided into three ethnic groups: Sami I (Sami used as home language in the last three generations), Sami II (at least one Sami identity marker) and Norwegian.SettingIn an area with Sami, Kven/Finnish and Norwegian populations, the SAMINOR study, 2003–2004.SubjectsA total of 5647 men and 6347 women aged 36–79 years.ResultsMore than 90 % of the study populations were coffee drinkers. Only 22 % were unfiltered coffee consumers. Sami I had the highest proportion of participants who consumed nine or more cups of unfiltered coffee per day, although the number of participants was limited. Total coffee consumption was associated with increased total cholesterol for men (P < 0·01) and women (P < 0·0001). For those who drank only unfiltered coffee, a significant association was found only in Norwegian men, adjusted for physical activity in leisure time, BMI and smoking habits (P < 0·001). From the lowest (less than five cups) to the highest (nine or more cups) unfiltered coffee consumption category, the mean total cholesterol levels increased by 0·29 mmol/l in Norwegian men.ConclusionsUnfiltered coffee consumption was lower in the present study compared to previous reports. In general, total coffee consumption was positively associated with total cholesterol levels. However, for unfiltered coffee consumption, an association was found only in Norwegian men.

scholarly journals Effect of poloxamer 407 administration on the serum lipids profile, anxiety level and protease activity in the heart and liver of mice

2013 ◽  
Vol 6 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Tatyana Korolenko ◽  
Thomas P. Johnston ◽  
Nina I. Dubrovina ◽  
Yana A. Kisarova ◽  
Svetlana Ya. Zhanaeva ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Morphological Changes ◽  
Cathepsin L ◽  
Chronic Administration ◽  
Cysteine Proteases ◽  
Heart Tissue ◽  
Poloxamer 407 ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Early Stages

Abstract Chronic administration of the poloxamer 407 (P-407), a block copolymer, to elevate serum lipids in mice is a well-established mouse model of hyperlipidemia and atherosclerosis. We tested the hypothesis that the activity of several types of proteases in heart and liver tissue is changed in the early stages of atherosclerosis development. Additionally, we evaluated whether increased serum lipids would induce anxiety in mice, as determined by using a ‘plus-maze’ test. The mice were administered P-407 by intraperitoneal injection twice a week for one month. P-407 administration to mice resulted in a marked increase in total serum cholesterol, atherogenic non-HDLcholesterol, and especially in total triglycerides, and it also increased anxiety. Morphological changes observed in P-407-treated mice included contractile type changes in cardiomyocytes and foamy macrophages in liver. A significant increase of cysteine proteases cathepsin B and cathepsin L (at 24 h) and aspartate protease cathepsin D (at both 24 h and 5 days) was determined in heart tissue following P-407 administration. However, no changes were noted in heart matrix metalloproteinase activity. The activity of cysteine and aspartate proteases was significantly increased in liver at both 24 hours and 5 days after P-407 administration. In conclusion, administration of P-407 to mice for one month resulted in increased anxiety, and more importantly, there was an increase in the activity of heart and liver proteases secondary to sustained dyslipidemia. It is suggested that heart and liver cysteine and aspartate proteases may represent potential therapeutic targets in the early stages of atherosclerosis.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK DAUN SALAM (EUGENIA POLIANTA) DIBANDINGKAN OBAT STATIN DALAM PENURUNAN KADAR KOLESTEROL TOTAL PADA PENDERTA HIPERKOLESTEROL DIWILAYAH KERJA UPTD PUSKESMAS KERINCI KANAN

2018 ◽  
Vol 5 (1) ◽  
pp. 1-8
Author(s):  
Yensasnidar Yensasnidar ◽  
Marlinda Marlinda
Keyword(s):  
Total Cholesterol ◽  
Leaf Extract ◽  
Capillary Blood ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Paired Sample ◽  
Is Research ◽  
Cholesterol Levels ◽  
Work Area ◽  
Statin Drug

So that monitoring and decreasing cholesterol level is important In general, this study aims to compare the effectiveness of salam leaf extract and statins on the decrease in total cholesterol levels in patients with hypercholesterolemia in the work area UPTD Puskesmas kerinci right 2017. This research is research quantitative with the research design used is Quasy Experimental Pretest-Postest Design by measuring total serum cholesterol levels before (Pretest) and after (Postest) Treatment given the extract of bay leaf and statin drug. This research was conducted in May 2017-Feb 2018 in UPTD area of Puskesmas Kerinci Right of Kerinci Kanan Sub-district of Siak Regency of Riau. The population in this study is hyperkolestterol patients who are in the working area of UPTD Puskesmas Kerinci Kanan Kerinci Kanan District Siak regency of Riau amounted to 12 people. The specimens examined from the sample were capillary blood to determine after addition of salam leaf extract and a 10mg statin drug. while for effectivity of giving of salam leaf extract was done paired sample with p = 0,001 with value Δ 38 while effectiveness of statin drug also paired sample with p = 0,028 with value Δ 20.

Total serum cholesterol and the energy status of cows

2021 ◽  
Vol 1 ◽  
pp. 59-64
Author(s):  
Ya.A. Zharikov ◽  
Keyword(s):  
Total Cholesterol ◽  
Milk Yield ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Energy Status ◽  
Lactate Dehydrogenase Activity ◽  
Energy Needs ◽  
Carbohydrate Components ◽  
Daily Milk Yield ◽  
Daily Milk

The relationship of total cholesterol with serum metabolites and cow productivity was investigated. To do this, the data-base (213 samples) was divided into six groups. The first group included samples from cows with total cholesterol levels above seven mmol/l, the second one – from six to seven, the third one – from five to six, the fourth one – from four to five, the fifth one – less than four and the sixth one – from non-dairy cows. A direct correlation (r = 0.44) of total cho-lesterol with daily milk yield was established. The average daily milk yield of cows consistently decreased from group to group – 23,6 – 19,2 – 18,6 – 15,9 – 16,3 l. In contrast to cholesterol, the values of the average glucose level were dis-tributed – 1,5 – 1,7 – 2,1 – 2,2 – 2,3 – 3,0 mmol/l (r = -0.31) and lactate dehydrogenase activity – 142,9 – 208,3 – 271,6 – 311,0 – 411,3 – 346,6 E/l (r = -0.45). The paper discusses the dynamics of cholesterol in relation to milk yield, physiological condition, and provision of energy needs of the cow's body by activating the lipid or carbohydrate components of metabolism.

Abstract P376: 40-year Population Trends in Cardiovascular Risk Factors in Finland

Circulation ◽  
2014 ◽  
Vol 129 (suppl_1) ◽  
Author(s):  
Katja Borodulin ◽  
Erkki Vartiainen ◽  
Markku Peltonen ◽  
Pekka Jousilahti ◽  
Anne Juolevi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Blood Pressure ◽  
Body Mass Index ◽  
Total Cholesterol ◽  
Diastolic Blood Pressure ◽  
Body Mass ◽  
Serum Cholesterol ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Chd Risk Factors

Background: Finland has experienced remarkable changes both in population levels of coronary heart disease risk factors and mortality over the past decades. For the national monitoring of risk factors in major non-communicable diseases, National FINRISK Studies have been implemented from 1972 to 2012. The 40-year changes in CHD risk factors are presented. Methods: Study population included participants aged 30 to 59 years in the series on independent random population samples. Data were collected in five-year intervals in 1972-2012. FINRISK studies so far comprised 53 589 men and women who participated in a health examination, gave a venous blood sample, and filled in questionnaires. Serum total cholesterol, systolic and diastolic blood pressure, and body mass index were measured using standardized protocol, and smoking status was recorded. Results: Total serum cholesterol decreased remarkably until the year 2007, but has thereafter turned to an increase. Systolic blood pressure has continued to decline over time since 1972, while decrease in diastolic blood pressure has leveled off during last ten years. Smoking prevalence has markedly decreased. Body mass index has increased in the population, yet the significant changes occurred in the earlier survey years, not in past 10 years. These changes in trends are shown in both sexes and all age groups in all geographical locations. Conclusions: After three decades of favorable development, the population risk factor levels showed some increase in total cholesterol and diastolic blood pressure. Some of these changes, such as in total serum cholesterol can be partially explained by unfavorable changes in dietary cholesterol intake. Our findings emphasize the need for continued efforts towards national disease prevention and health promotion.

Pengaruh Lamanya Penyimpanan Serum pada Suhu 20-80 0c Selama Satu Minggu terhadap Kadar Kolesterol Total

2017 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Laila Kamila ◽  
Slamet Slamet
Keyword(s):  
Total Cholesterol ◽  
Test Method ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Friedman Test ◽  
Chi Square ◽  
Cholesterol Levels ◽  
Laboratory Examinations ◽  
Pap Method ◽  
Serum Storage

Abstract: Laboratory examinations are essential to establish diseases diagnosis so the results should be accurate. Therefore, it is necessary to control the pre-analytic, analytic and post-analytic. Cholesterol examination is one of the ways to establish the diagnosis of DM, Heart and hypertension. This study was aimed to determine the effect of duration of serum storage at a temperature of 2°-8°C for a week with total cholesterol levels. Samples were checked daily for a week. The samples of this study were the 2nd and 3rd grade of Health Analyst’s students who lived in the male dormitory where total samples were 5 samples. The study was conducted from March to June 2013. Each sample was checked for total serum cholesterol levels which stored at a temperature of 2°-8°C for a week. This examination used CHOD-PAP method. From the results of data analysis with Friedman Test method, it was determined that total cholesterol levels in serum which stored at temperature 2°-8°C for a week obtained Chi-Square value = 28.827 and p = 0.000 where showed that there was effect of duration of serum storage at temperature 2°-8°C for a week with total cholesterol level.Abstrak: Pemeriksaan laboratorium sangat penting untuk menegakkan diagnosa penyakit sehingga hasil harus akurat. Oleh karena itu, perlu dilakukan pengendalian terhadap pra-analitik, analitik dan pasca analitik. Pemeriksaan kolesterol merupakan salah satu upaya untuk menegakkan diagnosa penyakit DM, Jantung, dan hipertensi. Penelitian ini bertujuan untuk mengetahui pengaruh lamanya penyimpanan serum pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total. Sampel diperiksa setiap hari selama 1 minggu. Sampel penelitian ini adalah mahasiswa tingkat 2 dan 3 Analis Kesehatan yang tinggal di asrama putra dengan jumlah sampel sebanyak 5 sampel. Penelitian ini dilaksanakan pada bulan Maret-Juni 2013. Masing-masing sampel diperiksa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu. Pemeriksaan ini menggunakan metode CHOD-PAP. Dari hasil analisis data dengan metode Friedman Test, diketahui bahwa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu didapatkan besaran nilai Chi Square = 28,827 dan p = 0.000. Hasil uji signifkansi Chi Square metode Friedman test menunjukkan bahwa p < 0.05 sehingga dapat disimpulkan bahwa terdapat pengaruh lamanya penyimpanan pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total.

Total serum cholesterol by isotope dilution/mass spectrometry: a candidate definitive method.

1980 ◽  
Vol 26 (7) ◽  
pp. 854-860 ◽  
Author(s):  
A Cohen ◽  
H S Hertz ◽  
J Mandel ◽  
R C Paule ◽  
R Schaffer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Total Cholesterol ◽  
Serum Cholesterol ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Weight Ratio ◽  
Molecular Ions ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Definitive Method

Abstract We describe a highly accurate and precise method for determination of total cholesterol in serum by isotope dilution/mass spectrometry. The method was developed for a Study Group of the Committee on Standards of the American Association for Clinical Chemistry, for use in establishing the accuracy of a candidate reference method for total cholesterol, and fulfills their criteria for a definitive method. Cholesterol-d7 is added to serum, with the weight ratio of cholesterol-d7 to total serum cholesterol kept near to 1:1. The esters are hydrolyzed and the cholesterol is separated and converted into the trimethylsilyl ether derivative for measurement by combined gas chromatography/mass spectrometry. The intensity ratio of the molecular ions at m/z 465 and 458 is measured for each sample and for two calibration mixtures, according to a prescribed bracketing protocol. A weight ratio for the sample is obtained by linear interpolation of the ion-intensity ratios, and the total cholesterol is then calculated. The method was applied four times over several weeks to each of five serum pools. Statistical analysis involving consideration of both replication error and variability between weeks gave a coefficient of variation for a single measurement of 0.36%. The absence of interferences in the method was demonstrated by measurements at several other masses.

Total cholesterol in serum determined by isotope dilution/mass spectrometry, with liquid-chromatographic separation.

1987 ◽  
Vol 33 (7) ◽  
pp. 1113-1117 ◽  
Author(s):  
A Takatsu ◽  
S Nishi
Keyword(s):  
Mass Spectrometry ◽  
Total Cholesterol ◽  
Isotope Dilution ◽  
Chromatographic Separation ◽  
Isotope Dilution Mass Spectrometry ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Liquid Chromatographic Separation ◽  
Liquid Chromatographic

Abstract We describe an accurate, precise method for determination of total serum cholesterol by isotope dilution/mass spectrometry (IDMS) with liquid-chromatographic separation. After adding [3,4-13C]cholesterol to serum and hydrolyzing the cholesterol esters, we extract the total cholesterol. "High-performance" liquid chromatography (HPLC) is used to separate the extracted cholesterol for measurement by electron-impact mass spectrometry with use of a direct-insertion device. To evaluate the specificity and the accuracy of this method, we also studied the conventional IDMS method, which involves converting cholesterol to the trimethylsilyl ether and assay by gas chromatography-mass spectrometry with use of a capillary column. The coefficient of variation for the HPLC method was a little larger than that for the conventional method, but mean values by each method agreed within 1% for all sera tested.

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