Interface Instrumentation between Computer and Spectrophotometer for Reaction Rate Measurements

1970 ◽  
Vol 16 (3) ◽  
pp. 215-221 ◽  
Author(s):  
E Clifford Toren ◽  
Arthur A Eggert ◽  
Arletta E Sherry ◽  
G Phillip Hicks
Keyword(s):  
Reaction Rate ◽  
Clinical Laboratory ◽  
Lactic Dehydrogenase ◽  
Input Voltage ◽  
Rate Of Change ◽  
Test Results ◽  
Laboratory Computer ◽  
Voltage Level ◽  
Accuracy And Precision ◽  
Software Changes

Abstract An instrument interface which generates an output voltage level proportional to rate of change of an input voltage is described. The interface permits direct connection of routine spectrophotometric rate measurements to a real-time laboratory computer system. The form of the voltage level output enables the computer to read rates in the same manner as peaks are read for AutoAnalyzers, permitting the clinical laboratory LABCOM system to compute and report results for rate methods with no software changes. Spectrophotometric rates from about 10 to 0.025 absorbance units per min can be measured with an accuracy and precision of less than 1% relative in measurement times of about 10 to 30 s, respectively. The interface is readily constructed from commercially available components. Test results are presented for simulated rates, lactic dehydrogenase, and alkaline phosphatase.

scholarly journals Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay

2021 ◽  
Vol 14 (3) ◽  
pp. 585-588
Author(s):  
Nutnaree Kunanusont ◽  
Darsaniya Punyadarsaniya ◽  
Sakchai Ruenphet
Keyword(s):  
Test Results ◽  
Serum Samples ◽  
Serum Progesterone ◽  
Percentage Difference ◽  
Accuracy And Precision ◽  
Standard Guideline ◽  
Significant Difference ◽  
Breeding Time ◽  
Chemiluminescent Microparticle Immunoassay ◽  
Mating Time

Background and Aim: The concentration of serum progesterone is commonly used to determine the optimal mating time in bitches, and to diagnose reproductive-related abnormalities. This study aims to compare the serum progesterone results obtained by rapid fluorescence immunochromatography assay (RFICA) with those obtained by chemiluminescent microparticle immunoassay (CMIA) from the same serum samples to develop a standard guideline for optimal breeding time. Materials and Methods: Serum progesterone levels were measured in 124 bitches using RFICA and CMIA. Simple linear regression and correlation analyses were performed to analyze the data. The percentage difference between the maximum and minimum progesterone values in the same serum sample in the same assay was compared using Wilcoxon's rank-sum test. Results: The present study showed a strong linear dependence of the results obtained by RFICA on those obtained by CMIA as R2=0.8976, with regression coefficient of 0.9474 and p<0.05, including the regression model was CMIA = (0.9483 × RFICA) - 0.761. Moreover, five critical measurement times during estrous in bitches showed statistically significant differences (p<0.05), except at the fertilizable period, which showed a non-significant difference (p>0.05). Conclusion: This study demonstrated that it is presumably acceptable to use the RFICA and CMIA methods interchangeably for quality progesterone measurements in serum samples from bitches. However, when considering the use of the RFICA method, it is advisable to carefully interpret the results and follow the interpretation guidelines. Finally, RFICA in the present study provides a reliable and convenient option for veterinarian practitioners to measure canine progesterone levels in-house.

scholarly journals Case Report: Two Cases of Subacute Thyroiditis Following SARS-CoV-2 Vaccination

2021 ◽  
Vol 8 ◽  
Author(s):  
Catherine Bornemann ◽  
Katharina Woyk ◽  
Caroline Bouter
Keyword(s):  
Viral Infections ◽  
Clinical Laboratory ◽  
Previous History ◽  
Subacute Thyroiditis ◽  
Clinical Test ◽  
Thyroid Disorders ◽  
Thyroid Disorder ◽  
Lateral Neck ◽  
Test Results ◽  
History Of

Subacute thyroiditis is an inflammatory thyroid disorder associated with viral infections. Rare cases of subacute thyroiditis have also been described following vaccination. Recently, a few cases of subacute thyroiditis following SARS-CoV-2 vaccination have also been reported. Here, we present two cases of cytological proven subacute thyroiditis after receiving the first dose of a SARS-CoV-2 vaccination. We describe clinical, laboratory, imaging and cytological findings in two cases of subacute thyroiditis that presented in our department 2 weeks after SARS-CoV-2 vaccination with Spikevax (Moderna Biotech, Spain) and Vaxzevria (AstraZeneca; Sweden). Both cases did not have a previous history of thyroid disorders and presented with anterior and lateral neck pain. Clinical test results as well as cytological findings were consistent with subacute thyroiditis. Subacute thyroiditis may develop following a SARS-CoV-2 vaccination and should be considered as a possible side effect in cases that present with thyroid pain.

scholarly journals Venous tromboembolic complications in stroke

2018 ◽  
pp. 70-77
Author(s):  
Екатерина Владимировна Силина ◽  
Е.Н. Кабаева ◽  
В.А. Ступин ◽  
А.А. Тяжельников ◽  
Т.Г. Синельникова ◽  
...  
Keyword(s):  
Pulmonary Embolism ◽  
Hemorrhagic Stroke ◽  
Clinical Laboratory ◽  
Disease Onset ◽  
Test Results ◽  
Thromboembolic Complications ◽  
Severe Stroke ◽  
Laboratory Markers ◽  
Venous Thromboembolic Events ◽  
Venous Thromboembolic

Актуальность работы обусловлена поиском путей улучшения результатов лечения больных инсультом. Цель исследования: выявить критерии прогноза риска развития венозных тромбозов, а также ключевые звенья патогенеза тромбоэмболических осложнений у пациентов с острым инсультом. Материалы и методы: в проспективное исследование включено 145 больных с инсультом (104 с ишемическим (ИИ) и 41 с геморрагическим (ГИ)), госпитализированных в отделение нейрореанимации в период 3,5-24 часа от начала заболевания и имеющих на момент включения в исследование различную степень депрессии сознания (тяжелая степень инсульта). Пациентам проводилась терапия в соответствии со стандартами оказания медицинской помощи, согласно которым всем пациентам назначали антикоагулянтную терапию (АКТ). Выполняемый в динамике стандартный клинико-диагностический и лабораторный мониторинг был дополнен тестом «Тромбодинамика». Результаты: у 95% пациентов с инсультом зарегистрированы различные факторы риска венозных тромбоэмболических осложнений (ВТЭО). Тромбоэмболия легочной артерии (ТЭЛА) развилась в 24% случаев, преимущественно на 2-3 неделе, в среднем через 6 дней после отмены АКТ. Описана динамика и признаки дисбаланса в системе гемостаза у больных инсультом, нараставшие после отмены АКТ. Показано, что стандартные методы исследования системы гемостаза по сравнению с прямым методом менее информативны для выявления ВТЭО и оценки эффективности АКТ. Вероятность развития ВТЭО прямо пропорциональна скорости смены состояния гиперкоагуляции состоянием гипокоагуляции. При этом состояние фоновой гиперкоагуляции не коррелирует с развитием ВТЭО. Корреляционный анализ изменений в системе гемостаза с динамикой клинико-лабораторных маркеров у больных с тяжелым инсультом выявил закономерные изменения показателей коагуляционного гемостаза в условиях реализации разных схем стандартной АКТ. Эти схемы были сопоставимы по содержанию при развитии как ВТЭО и ТЭЛА, так и геморрагических осложнений. Вывод: К больным инсультом необходим персонализированный подход при динамическом мониторировании гемостаза и назначении антикоагулянтной терапии. This work was warranted by the need to improve results in the treatment of stroke. The aim of this study was to identify criteria for predicting the risk of venous thrombosis and to elucidate the pathogenesis of thromboembolic complications in patients with acute stroke. Materials and methods. This prospective study included 145 patients (104 patients with ischemic stroke and 41 patients (28.3%) with hemorrhagic stroke). All patients were hospitalized to the neuroresuscitation unit within 3.5 to 24 hours of the disease onset at different stages of consciousness impairment. The patients received anticoagulant therapy (ACT) according to current healthcare standards. Standard clinical diagnostic and laboratory monitoring was supplemented with a Thrombodynamics test. Results. Risk factors for venous thromboembolic events (VTE) were observed in 95% of patients. Pulmonary embolism developed in 24% of cases mostly during weeks 2-3, generally at 6 days of ACT withdrawal. Hemostatic changes and disbalance progressed after the ACT withdrawal. Standard methods of studying hemostasis were shown to be less informative in detecting VTE and evaluating ACT efficacy than the thermodynamics method. The probability of VTE was directly proportional to the velocity of hypercoagulation transformation into hypocoagulation. In this process, the background hypocoagulation was not correlated with the development of VTE. Analysis of correlations of hemostasis changes with changes in clinical-laboratory markers identified relationships of changes in coagulation hemostasis with different standard ACT programs. VTE, pulmonary embolism, and hemorrhagic complications developed in association with administration of comparable ACT programs to patients with severe stroke.

An Evaluation of the Greiner Selective Analyzer and its Role in the Clinical Laboratory

1975 ◽  
Vol 12 (1-6) ◽  
pp. 182-191 ◽  
Author(s):  
Andrew G. Skinner ◽  
Peter Wilding
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Creatinine ◽  
Total Protein ◽  
Total Bilirubin ◽  
Clinical Laboratory ◽  
Comparison Of Results ◽  
Operation Temperature ◽  
Accuracy And Precision ◽  
Analytical Range

The Greiner Selective Analyzer (GSA II) was evaluated over a period of six months. The evaluation assessed the reliability, accuracy, and precision of the analyser for six determinations. The methods evaluated were for glucose, urea, creatinine, total protein, total bilirubin, and alkaline phosphatase. Comparison of results was also made with those obtained for the same specimens using the Technicon SMA 12/60 Analyzer. Correlation and comparison of results indicate that the Greiner Selective Analyzer performed better for three of the methods but worse for serum creatinine determination. The role of the analyser as a routine tool in the clinical laboratory was also evaluated during analyses of approximately 900 patient specimens. Other features evaluated were analytical range of the six methods under study, the economics of operation, temperature control, and electrical and mechanical safety.

Herbal Remedies: Effects on Clinical Laboratory Tests

2006 ◽  
Vol 130 (4) ◽  
pp. 521-528 ◽  
Author(s):  
Amitava Dasgupta ◽  
David W. Bernard
Keyword(s):  
Chinese Medicine ◽  
Laboratory Test ◽  
Alternative Medicine ◽  
Clinical Laboratory ◽  
Herbal Remedies ◽  
Test Results ◽  
Abnormal Laboratory ◽  
St John's Wort ◽  
Laboratory Test Results ◽  
Laboratory Results

AbstractContext.—Complementary and alternative medicine (herbal medicines) can affect laboratory test results by several mechanisms.Objective.—In this review, published reports on effects of herbal remedies on abnormal laboratory test results are summarized and commented on.Data Sources.—All published reports between 1980 and 2005 with the key words herbal remedies or alternative medicine and clinical laboratory test, clinical chemistry test, or drug-herb interaction were searched through Medline. The authors' own publications were also included. Important results were then synthesized.Data Synthesis.—Falsely elevated or falsely lowered digoxin levels may be encountered in a patient taking digoxin and the Chinese medicine Chan Su or Dan Shen, owing to direct interference of a component of Chinese medicine with the antibody used in an immunoassay. St John's wort, a popular herbal antidepressant, increases clearance of many drugs, and abnormally low cyclosporine, digoxin, theophylline, or protease inhibitor concentrations may be observed in a patient taking any of these drugs in combination with St John's wort. Abnormal laboratory results may also be encountered owing to altered pathophysiology. Kava-kava, chaparral, and germander cause liver toxicity, and elevated alanine aminotransferase, aspartate aminotransferase, and bilirubin concentrations may be observed in a healthy individual taking such herbal products. An herbal product may be contaminated with a Western drug, and an unexpected drug level (such as phenytoin in a patient who never took phenytoin but took a Chinese herb) may confuse the laboratory staff and the clinician.Conclusions.—Use of alternative medicines may significantly alter laboratory results, and communication among pathologists, clinical laboratory scientists, and physicians providing care to the patient is important in interpreting these results.

The Use of Laboratory Computers in Monitoring Kinetic Enzyme Assays

1973 ◽  
Vol 19 (1) ◽  
pp. 27-30 ◽  
Author(s):  
G Phillip Hicks ◽  
R A Ziesemer ◽  
Norbert W Tietz
Keyword(s):  
Computer Program ◽  
Computer System ◽  
Kinetic Data ◽  
Clinical Laboratory ◽  
Lag Phase ◽  
Enzyme Assays ◽  
Data Handling ◽  
Laboratory Computer ◽  
Linear Portion ◽  
Complete Automation

Abstract An online computer program to monitor kinetic enzyme assays is described. The program analyzes the kinetic data in a manner similar to the way a technologist handles data in a manual procedure, taking into account the lag phase, substrate depletion phase, and linear portion of the rate curve. Thus, complete automation of even complex kinetic assays has been made practical. The program has been implemented for routine use in a clinical laboratory computer system ("LABCOM"), and the results correlate well with those obtained by established methods of manual data-handling procedures

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