Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay

Background and Aim: The concentration of serum progesterone is commonly used to determine the optimal mating time in bitches, and to diagnose reproductive-related abnormalities. This study aims to compare the serum progesterone results obtained by rapid fluorescence immunochromatography assay (RFICA) with those obtained by chemiluminescent microparticle immunoassay (CMIA) from the same serum samples to develop a standard guideline for optimal breeding time. Materials and Methods: Serum progesterone levels were measured in 124 bitches using RFICA and CMIA. Simple linear regression and correlation analyses were performed to analyze the data. The percentage difference between the maximum and minimum progesterone values in the same serum sample in the same assay was compared using Wilcoxon's rank-sum test. Results: The present study showed a strong linear dependence of the results obtained by RFICA on those obtained by CMIA as R2=0.8976, with regression coefficient of 0.9474 and p<0.05, including the regression model was CMIA = (0.9483 × RFICA) - 0.761. Moreover, five critical measurement times during estrous in bitches showed statistically significant differences (p<0.05), except at the fertilizable period, which showed a non-significant difference (p>0.05). Conclusion: This study demonstrated that it is presumably acceptable to use the RFICA and CMIA methods interchangeably for quality progesterone measurements in serum samples from bitches. However, when considering the use of the RFICA method, it is advisable to carefully interpret the results and follow the interpretation guidelines. Finally, RFICA in the present study provides a reliable and convenient option for veterinarian practitioners to measure canine progesterone levels in-house.

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Comparative assesment of accuracy of IOPA and CBCT for maxillary molar furcation involvement: a clinical and radiological study

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20181776 ◽

2018 ◽

Vol 6 (5) ◽

pp. 1765 ◽

Cited By ~ 1

Author(s):

Mohammed Fahad Parvez ◽

Nandini Manjunath ◽

Raghavendra Kini

Keyword(s):

Surgical Intervention ◽

Three Dimensional ◽

Test Results ◽

Two Dimensional Image ◽

Accuracy And Precision ◽

Significant Difference ◽

Maxillary Molar ◽

Furcation Involvement ◽

Dimensional Object ◽

Periodontal Bone Defects

Background: Radiographs in periodontics will provide a two-dimensional image of a three-dimensional object and this leads to problems in terms of validity, accuracy and precision. Hence, the study was conducted with an aim to assess the accuracy of IOPA And CBCT for Maxillary Molar Furcation involvement by clinical and radiological methods.Methods: The study sample consisted of 17 subjects from the Out patient Department of A.J. Institute of Dental Science, Manglore belonging to both sexes and with age ranging from 35-55 years. The selected patients were those having presence of periodontal pockets of >5-6mm and advanced periodontal disease requiring surgical intervention. For the purpose of standardization, 3 sites of the maxillary first molars were assessed-Mid-buccal, Mesio-buccal, Disto-buccal. Iopa and CBCT measurements were taken which was followed by surgical intervention during which probing measurements were recorded. Statistical analysis was done using ‘unpaired t test’.Results: There was no statistically significant difference between CBCT measurements and surgical measurements (p≤0.05).Conclusions: CBCT accurately reproduced the clinical measurement of periodontal bone defects.

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X-Ray Spectroscopy in the Clinical Laboratory

Clinical Chemistry ◽

10.1093/clinchem/6.4.299 ◽

1960 ◽

Vol 6 (4) ◽

pp. 299-313 ◽

Cited By ~ 19

Author(s):

Samuel Natelson ◽

Bertrum Sheid

Keyword(s):

Clinical Laboratory ◽

Electrophoretic Pattern ◽

Total Sulfur ◽

Serum Samples ◽

X Ray ◽

Content Ratio ◽

Accuracy And Precision ◽

Significant Difference ◽

Before And After ◽

Operation Results

Abstract Chlorine and sulfur are assayed in serum with the X-ray spectrometer. The serum samples are dried in confined spots on a long strip of filter paper. The strip is passed continuously through the X-ray field and the element assayed by the peak of the curve drawn on the recorder. An attachment for the spectrometer is described for this purpose, as well as an instrument for making the confined spots. Results for chlorine in serum are compared with those obtained by mercurimetric titration. The results are comparable as regards accuracy and reproducibility. Using 20 µ1. of serum and the automatic feed, the results for chlorine indicate a reproducibility, by X-ray, of ± 3.4 per cent (2σ). No significant difference was observed in comparing the values for sulfur obtained by X-ray with those obtained by ashing and treatment with barium chloranilate. The X-ray technic with 25 µ1. of serum is reproducible to ± 2.2 per cent (2σ). Serum was dialyzed against saline and assayed for sulfur content before and after dialysis. The dialyzable sulfur is small in comparison with the total protein sulfur. In comparing normal and pathologic sera with respect to their sulfur-to-protein content ratio it is observed that this varies apparently because the serum globulins contain amounts of sulfur different from serum albumin. The abnormal protein observed in multiple myeloma contains less than half the sulfur of the albumin from the same patient. This was determined by scanning the electrophoretic pattern of the serum for sulfur and protein. It is pointed out that the X-ray technic is practical for routine analysis in the clinical laboratory in that no reagents are required for total sulfur, chlorine, calcium and potassium; furthermore, only an ultramicrosample is needed and the instrument is easily adapted to automatic operation. Results are comparable with those obtained by conventional methods with regards to accuracy and precision.

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Perbedaan sensitivitas tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada otitis media supuratif kronik

Oto Rhino Laryngologica Indonesiana ◽

10.32637/orli.v42i2.22 ◽

2012 ◽

Vol 42 (2) ◽

Author(s):

Anton Budhi Darmawan ◽

Dwi Utami Anjarwati

Keyword(s):

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Chronic Suppurative Otitis Media ◽

Test Results ◽

Sectional Study ◽

Suppurative Otitis Media ◽

Number Of Patients ◽

Significant Difference ◽

Cochran Test ◽

Post Hoc

Background: Chronic suppurative otitis media (CSOM) is one infectious disease of the middle ear, most commonly caused by Pseudomonas aeruginosa. A high number of patients come to the ENT outpatient clinic with active benign type of CSOM. The bacteria Pseudomonas aeruginosa is capable of producing biofilm which protects itself from penetration of antibiotics, and therefore creates resistance towards antibiotics and difficult to eradicate. Objective: The aim of this study was to compare the sensitivity levels of chloramphenicol, polymyxin-neomycin, cyprofloxacin and ofloxacine against Pseudomonas aeruginosa in patients with active benign type CSOM in ENT clinic. Method: The method used was across sectional study on 25 patients, from August 2010 until December 2010. Samples were taken withear swab and then put on sensitivity test to chloramphenicol, polymyxin-neomycin, cyprofloxacin andofloxacine using the diffusion disc method. The analysis used in this study was Cochran test. Results: Results showed a significant difference in sensitivity among chloramphenicol (38,70%), polymyxinneomycin(83,87%),cyprofloxacin(90,32%)andofloxacin(58,06%)withp=0,000(p<0,05).PostHocanalysisusing the Mc Nemar indicated that there were significant differences in sensitivity betweenpolymyxin-neomycin to chloramphenicol with p=0,000 (p<0,05), ciprofloxacin to chloramphenicol andciprofloxacin to ofloxacine with p= 0,002, but there were no significant differences between cyprofloxacinto polymyxin-neomycin with p=0,687, polymyxin-neomycin to ofloxacin p=0.057 and ofloxacin tochloramphenicol p=0,109. There were significant differences in antibiotic ear dropssensitivity to Pseudomonas aeruginosa in patients with active benign type of CSOM. Cyprofloxacin andpolymyxin-neomycin were more sensitive than ofloxacin and chloramphenicol. Keywords: Pseudomonas aeruginosa, active benign type of chronic suppurative otitis media, antibioticear drops. Abstrak : Latar belakang: Otitis media supuratif kronik (OMSK) merupakan penyakit infeksi kronik telinga tengah yang sering dijumpai di klinik THT. Penyebab tersering OMSK adalah bakteri Pseudomonasaeruginosa. Pseudomonas aeruginosa mempunyai kemampuan untuk membentuk biofilm yangmelindunginya dari penetrasi antibiotik sehingga menimbulkan resistensi terhadap antibiotik dan sulituntuk eradikasinya. Tujuan: Untuk mengetahui perbandingan tingkat sensitivitas kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin terhadap isolat Pseudomonas aeruginosa padapasien OMSK benigna aktif di klinik THT RSMS. Metode: Metode yang digunakan adalah crosssectional terhadap 29 pasien OMSK di klinik THT RSMS periode bulan Agustus 2010 - Desember2010. Pengambilan sampel dilakukan dengan swab telinga. Uji sensitivitas terhadap kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin dilakukan dengan metode cakram secara difusi.Analisis yang digunakan dalam penelitian ini adalah Cochran dan analisis post hoc. Hasil: Didapatkansensitivitas kloramfenikol sebesar 38,70%, polimiksin-neomisin sebesar 83,87%, ciprofloksasin sebesar90,32% dan ofloksasin sebesar 58,06% dengan p=0,01 (P<0,05), yang menunjukkan adanya perbedaansensitivitas yang bermakna antara kloramfenikol, polimiksin-neomisin, ciprofloksasin dan ofloksasinterhadap Pseudomonas aeruginosa. Analisis post hoc menggunakan Mc Nemar menunjukkan bahwaterdapat perbedaan sensitivitas yang bermakna antara tetes telinga polimiksin-neomisin terhadapkloramfenikol, dan ciprofloksasin terhadap kloramfenikol p=0,000 (p<0,05), serta terdapat perbedaanyang bermakna antara tetes telinga ciprofloksasin terhadap ofloksasin, p=0,002, tetapi tidak terdapatperbedaan yang bermakna antara ciprofloksasin terhadap polimiksin-neomisin, p=0,687, polimiksinneomisinterhadap ofloksasin p=0,057, dan kloramfenikol terhadap ofloksasin p=0,109. Kesimpulan:Terdapat perbedaan sensitivitas yang bermakna tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada pasien OMSK benigna aktif. Ciprofloksasin dan polimiksin-neomisin tetes telinga mempunyai sensitivitas yang lebih baik dibanding ofloksasin dan kloramfenikol. Kata kunci: Pseudomonas aeruginosa, otitis media supuratif kronik, tetes telinga antibiotik

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A STUDY OF CONDYLAR POSITION IN CENTRIC RELATION AND MAXIMAL INTERCUSPATION USING CONEBEAM TOMOGRAPHY

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2019.2.14 ◽

2019 ◽

pp. 84-92

Author(s):

Anh Chi Phan ◽

Xuan Anh Ngoc Ho

Keyword(s):

Dental Students ◽

Cone Beam ◽

Test Results ◽

Reference Position ◽

Centric Relation ◽

Temporomandibular Joints ◽

Significant Difference ◽

Mandibular Fossa ◽

Condylar Position ◽

Cone Beam Tomography

Background: The condylar position discrepancy between centric relation and maximal intercuspation has been still a controversial issue. Aims: To compare the condylar position between centric relation and maximal intercuspation using cone-beam tomography in patients without temporomandibular joints disorder. Materials and methods: To assess the condylar position in centric relation and maximal intercuspation using cone-beam tomography on 40 fifth-year and sixth-year dental students of Hue University of Medicine and Pharmacy without temporomandibular joints disorder. The condylar positions are assessed following Sener classification (2009) and are compared between centric relation and maximal intercuspation using pairedsamples t-test and Mann-Whitney U-test. Results: Among 480 condye-to-fossa measurement pairs, there are 91.2% pairs having difference between two reference position but there is no significant difference. The condylar position at the superior of mandibular fossa has the greatest percentage in both centric relation and maximal intercuspation (43.8% in centric relation and 51.2% in maximal intercuspation). This greatest percentage is followed by the condylar position at posterior of mandibular fossa (32.5% in centric relation and 36.3% in maximal intercuspation). Lastly, the condylar position at the anterior of mandibular fossa has the fewest percentage (23.7% in centric relation and 12.5% in maximal intercuspation). Conclusion: There is no significant difference of condylar position between centric relation and maximal intercuspation in patients without temporomandibular joints disorder. Key words: Condylar position, centric relation, maximal intercuspation, cone-beam tomography

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Reassessing the Role of the Active TGF-β1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations

Disease Markers ◽

10.1155/2016/6064830 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Andréa Tavares Dantas ◽

Sayonara Maria Calado Gonçalves ◽

Anderson Rodrigues de Almeida ◽

Rafaela Silva Guimarães Gonçalves ◽

Maria Clara Pinheiro Duarte Sampaio ◽

...

Keyword(s):

Systemic Sclerosis ◽

Clinical Manifestations ◽

Serum Levels ◽

Vascular Involvement ◽

Digital Ulcers ◽

Serum Samples ◽

Peripheral Blood Mononuclear ◽

Pbmc Culture ◽

Significant Difference ◽

Skin Biopsies

Objective. To determine active TGF-β1 (aTGF-β1) levels in serum, skin, and peripheral blood mononuclear cell (PBMC) culture supernatants and to understand their associations with clinical parameters in systemic sclerosis (SSc) patients.Methods. We evaluated serum samples from 56 SSc patients and 24 healthy controls (HC). In 20 SSc patients, we quantified spontaneous or anti-CD3/CD28 stimulated production of aTGF-β1 by PBMC. The aTGF-β1 levels were measured by ELISA. Skin biopsies were obtained from 13 SSc patients and six HC, and TGFB1 expression was analyzed by RT-PCR.Results. TGF-β1 serum levels were significantly higher in SSc patients than in HC (p< 0.0001). Patients with increased TGF-β1 serum levels were more likely to have diffuse subset (p= 0.02), digital ulcers (p= 0.02), lung fibrosis (p< 0.0001), positive antitopoisomerase I (p= 0.03), and higher modified Rodnan score (p= 0.046). Most of our culture supernatant samples had undetectable levels of TGF-β1. No significant difference in TGFB1 expression was observed in the SSc skin compared with HC skin.Conclusion. Raised active TGF-β1 serum levels and their association with clinical manifestations in scleroderma patients suggest that this cytokine could be a marker of fibrotic and vascular involvement in SSc.

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Sinonasal effects of external dacryocystorhinostomy

The Journal of Laryngology & Otology ◽

10.1017/s002221512100181x ◽

2021 ◽

pp. 1-4

Author(s):

H Eyigor ◽

E A Cetinkaya ◽

D T Coban ◽

G Ozturk ◽

Ö Erdem

Keyword(s):

Mucociliary Clearance ◽

Nasal Symptom ◽

Ciliary Activity ◽

Test Results ◽

Saccharine Test ◽

Significant Difference ◽

Symptom Scores ◽

Endoscopic Score ◽

External Dacryocystorhinostomy

Abstract Objective External dacryocystorhinostomy is thought to cause mucociliary dysfunction by damaging the mucosa, in turn affecting ciliary activity and mucus quality. This study investigated the effect of external dacryocystorhinostomy on sinonasal function. Methods Patients scheduled for unilateral external dacryocystorhinostomy who underwent endoscopic nasal examination and paranasal sinus computed tomography were included in this study. A saccharine test was performed on the planned surgical side and the mucociliary clearance time was determined. The sinonasal quality of life was measured in all patients, pre-operatively and at six months post-operatively, using the Sino-Nasal Outcome Test-22. The Lund–Kennedy endoscopic score was also determined in all patients, both pre- and post-operatively. Results The study comprised 28 patients (22 females and 6 males). A statistically significant difference was found between the pre- and post-operative saccharine test results (p = 0.006), but not between the pre- and post-operative Sino-Nasal Outcome Test-22 scores (p > 0.05). Conclusion This study is one of only a few to investigate the effect of external dacryocystorhinostomy on sinonasal function. The results showed that external dacryocystorhinostomy impairs mucociliary clearance. The surgical procedure is well tolerated and does not significantly change nasal symptom scores.

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THU0296 PROTEIN PROFILING IN INDIVIDUALS BEFORE ONSET OF ANCA-ASSOCIATED VASCULITIS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.3033 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 376.2-376

Author(s):

E. Berglin ◽

A. Esberg ◽

J. Dahlqvist ◽

J. Sjöwall ◽

A. Lundquist ◽

...

Keyword(s):

Immune System ◽

Growth Factor ◽

Random Forest ◽

Symptom Onset ◽

Partial Least Square ◽

Protein Profiling ◽

Serum Samples ◽

Anca Associated Vasculitis ◽

Significant Difference ◽

Novel Biomarkers

Background:Etiology and pathogenesis of ANCA-associated vasculitis (AAV) is multifactorial and understanding of the processes leading from a healthy immune system to autoimmunity and on to debut of symptoms in AAV is rudimentary.Objectives:To identify inflammatory proteins related to the early processes preceding AAV development, and potential novel biomarkers, using large-scale protein analysesMethods:The Swedish National Patient Register of in-patient carevand the Swedish Cause of Death Register with discharge diagnosis from ICD-9 and-10 for AAV were co-analysed with the registers of 4 different blood biobanks to identify AAV individuals with available samples predating onset of symptom. Of the pre-AAV cases 86 (36 male, 50 female; mean age (SD); 51.9 (16.9) years) were identified with at least one plasma or serum sample (28 plasma, and 100 serum) pre-dating symptom onset (mean (SD); -4.3 (3.1) years), and 14 had 2-3 samples. Serum and plasma control samples matched for sex, age and sampling date were identified (n=198; 82 male, 116 female; mean age (SD); 51.9±15.9 years). The samples were analysed for levels of 92 proteins using proximity extension assay (OLINK inflammation panel, SciLifeLab, Uppsala, Sweden). Data were analysed using routine statistical methods, random forest and Partial Least square-discriminant analysis (PLS-DA).Results:As previously described for the assay significant difference between plasma and serum samples were observed both in pre-AAV individuals and controls. In pre-AAV plasma samples significantly increased concentrations of interleukin (IL)-2, chemokine ligand (CCL)-4, fibroblast growth factor (FGF)21, IL-4 and CCL20 were found closer to symptom onset, (<5 years) than later (> 5 years) and compared with controls. In serum tumor necrosis factor receptor superfamily member (TNFRSF)9, CXCL9, osteoprotegerin and vascular endothelial growth factor-A were significantly increased <5 years before onset vs. later (>5 years) and compared with controls. PLS-DA score scattered plot separated the pre-AAV individuals from healthy controls (R2=0.26), with significantly increased levels of CCL23, CXCL5, and matrix metalloproteinases-1 (MMP-1),transforming growth factor-ß, orosomucoid, en-rage (S100A12) and IL-7 and decreased FGF-19 level in serum. Binary logistic regression analyses comparing tertiles for these proteins confirmed significantly increased odds ratios for disease development of CCL23, CXCL5 and MMP-1. The findings were confirmed in random forest analysis where these factors were among the 20 most discriminatory factors between pre-symptomatic AAV and controls.Conclusion:In serum samples collected years before symptom onset of AAV, proteins involved in immune system activation were increased, suggesting that the inflammatory process is initiated long before clinical manifestations of the disease appear. These findings propose the elevated proteins as novel biomarkers for disease progression.References:[1]Watts et al. Ann Rheum Dis 2007;66:222-22Acknowledgments:Vasculitis Foundation, USADisclosure of Interests:Ewa Berglin: None declared, Anders Esberg: None declared, Johanna Dahlqvist: None declared, Johanna Sjöwall: None declared, Anders Lundquist: None declared, Kristina Lejon: None declared, Ingegerd Johansson: None declared, Aladdin J Mohammad Speakers bureau: lecture fees from Roche and Elli Lilly Sweden, PI (GiACTA study), Solbritt Rantapää Dahlqvist: None declared

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The value of serum progesterone level on day of human chorionic gonadotrophin administration / metaphase II oocyte ratio in predicting IVF/ICSI outcome in patients with normal ovarian reserve

Journal of Ovarian Research ◽

10.1186/s13048-021-00800-5 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Ahmad Mahran ◽

Mohammed Khairy ◽

Reham Elkhateeb ◽

Abdel Rahman Hegazy ◽

Ayman Abdelmeged ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Ovarian Response ◽

Pregnancy Rates ◽

Clinical Pregnancy ◽

Progesterone Level ◽

Receiver Operating Curve ◽

Serum Progesterone ◽

Predictive Values ◽

Significant Difference ◽

Assisted Reproduction Treatment

Abstract Background The clinical implication of the increased serum progesterone level on the day of HCG administration in assisted reproduction treatment (ART) is still controversial. The current study aimed to compare the predictive value of serum progesterone on day of HCG administration / metaphase II oocyte (P/MII) ratio on IVF/ ICSI outcome to serum progesterone (P) level alone and the ratio of serum progesterone/estradiol level (P/E2) ratio in prediction of pregnancy rates after ART. Material & methods Two hundred patients admitted to the IVF/ICSI program at Minia IVF center in Egypt in the period from October 2016 to May 2018 were included in this study. Serum Progesterone (P) and Estradiol (E2) levels were estimated on the day of HCG administration. The ratio between serum P and the number of MII oocytes (P/MII ratio) was calculated and the predictive values of the three parameters (P, P/E2 ratio and P/MII ratio) in prediction of cycle outcomes were measured. Results P/ MII oocyte ratio was significantly lower in patients who attained clinical pregnancy (n = 97) as compared with those who couldn’t whilst there was no significant difference in P and P/E2 ratio between the two groups. Using a cut off value of 0.125, the sensitivity and specificity of progesterone/ MII ratio in prediction of no pregnancy in IVF/ICSI were 75.7 and 77.1% respectively with the area under The Receiver operating curve (ROC-AUC) = 0.808. The respective values of the ROC-AUC for the P and P/E2 ratio were 0.651 and 0.712 with sensitivity and specificity of 71.2 and 73.5%for P level and of 72.5 and 75.3% for P/E2 ratio. Implantation or clinical pregnancy rates were significantly different between patients with high and low P/MII ratio irrespective of day of embryo transfer (day 3 or 5). Conclusions In patients with normal ovarian response, serum progesterone on day of HCG / MII oocyte ratio can be a useful predictor of pregnancy outcomes and in deciding on freezing of all embryos for later transfer instead of high progesterone level alone.

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Long-Term Longitudinal Evaluation of Six Commercial Immunoassays for the Detection of IgM and IgG Antibodies against SARS CoV-2

Viruses ◽

10.3390/v13071244 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1244

Author(s):

Iulia Nedelcu ◽

Raluca Jipa ◽

Roxana Vasilescu ◽

Cristian Băicuș ◽

Costin-Ioan Popescu ◽

...

Keyword(s):

Clinical Performance ◽

Sample Selection ◽

Early Time ◽

Humoral Response ◽

High Specificity ◽

Data Interpretation ◽

Serum Samples ◽

Chemiluminescent Immunoassay ◽

Chemiluminescent Microparticle Immunoassay ◽

Antibody Levels

The number of serological assays for SARS-CoV-2 has skyrocketed in the past year. Concerns have been raised regarding their performance characteristics, depending on the disease severity and the time of the analysis post-symptom onset (PSO). Thus, independent validations using an unbiased sample selection are required for meaningful serology data interpretation. We aimed to assess the clinical performance of six commercially available assays, the seroconversion, and the dynamics of the humoral response to SARS-CoV-2 infection. The study included 528 serum samples from 156 patients with follow-up visits up to six months PSO and 161 serum samples from healthy people. The IgG/total antibodies positive percentage increased and remained above 95% after six months when chemiluminescent immunoassay (CLIA) IgG antiS1/S2 and electro-chemiluminescent assay (ECLIA) total antiNP were used. At early time points PSO, chemiluminescent microparticle immunoassay (CMIA) IgM antiS achieved the best sensitivity. IgM and IgG appear simultaneously in most circumstances, and when performed in parallel the sensitivity increases. The severe and the moderate clinical forms were significantly associated with higher seropositivity percentage and antibody levels. High specificity was found in all evaluated assays, but the sensitivity was variable depending on the time PSO, severity of disease, detection method and targeted antigen.

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Quantification and Classification of Diclofenac Sodium Content in Dispersed Commercially Available Tablets by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Pharmaceuticals ◽

10.3390/ph14050440 ◽

2021 ◽

Vol 14 (5) ◽

pp. 440

Author(s):

Eirini Siozou ◽

Vasilios Sakkas ◽

Nikolaos Kourkoumelis

Keyword(s):

Infrared Spectroscopy ◽

Low Cost ◽

Diclofenac Sodium ◽

Reference Method ◽

Sodium Content ◽

Attenuated Total Reflection ◽

Accuracy And Precision ◽

Significant Difference ◽

Ft Ir

A new methodology, based on Fourier transform infrared spectroscopy equipped with an attenuated total reflectance accessory (ATR FT-IR), was developed for the determination of diclofenac sodium (DS) in dispersed commercially available tablets using chemometric tools such as partial least squares (PLS) coupled with discriminant analysis (PLS-DA). The results of PLS-DA depicted a perfect classification of the tablets into three different groups based on their DS concentrations, while the developed model with PLS had a sufficiently low root mean square error (RMSE) for the prediction of the samples’ concentration (~5%) and therefore can be practically used for any tablet with an unknown concentration of DS. Comparison with ultraviolet/visible (UV/Vis) spectrophotometry as the reference method revealed no significant difference between the two methods. The proposed methodology exhibited satisfactory results in terms of both accuracy and precision while being rapid, simple and of low cost.

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