Gas-Chromatographic and Mass-Spectrometric Detection of Low-Molecular-Weight Aliphatic Alcohols in Urine of Normal Individuals and Patients with Diabetes Mellitus

1975 ◽  
Vol 21 (9) ◽  
pp. 1294-1296 ◽  
Author(s):  
Hartmut M Liebich ◽  
Ossama Al-Babbili ◽  
Albert Zlatkis ◽  
Kyoung Kim
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Urinary Metabolites ◽  
Mass Spectrometric ◽  
Aliphatic Alcohols ◽  
Porous Polymer ◽  
Low Molecular Weight ◽  
Primary Alcohols ◽  
Special Diet ◽  
Patients With Diabetes

Abstract We studied the aliphatic alcohols in 100 urines from 25 patients with diabetes mellitus under treatment with insulin, oral antidiabetic medication, or special diet. The procedure involves adsorption of the low-molecular-weight urinary metabolites on a porous polymer of 2,6-diphenyl-p-phenylene oxide (Tenax GC), gas-chromatographic separation, mass spectrometric identification, and mass fragmentographic representation of the primary alcohols by a computer. The concentrations of ethanol, n-propanol, isobutanol, n-butanol, and isopentanol are increased as compared with urine from normal persons.

Soluble Fibrin Complexes and Fibrinogen Heterogeneity in Diabetes mellitus

1980 ◽  
Vol 44 (03) ◽  
pp. 130-134 ◽  
Author(s):  
E B Tsianos ◽  
N E Stathakis
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Plasma Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Lower Molecular Weight ◽  
Polyacrylamide Gels ◽  
Soluble Fibrin ◽  
Α2 Macroglobulin ◽  
Patients With Diabetes

SummaryThe presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.

A study of high-, middle- and low-molecular weight adiponectin in urine as a surrogate marker for early diabetic nephropathy using ultrasensitive immune complex transfer enzyme immunoassay

2018 ◽  
Vol 55 (5) ◽  
pp. 525-534 ◽  
Author(s):  
Mayumi Yamamoto ◽  
Yuki Fujimoto ◽  
Shino Hayashi ◽  
Seiichi Hashida
Keyword(s):  
Molecular Weight ◽  
Diabetic Nephropathy ◽  
Enzyme Immunoassay ◽  
Immune Complex ◽  
Healthy Subjects ◽  
Surrogate Marker ◽  
Urinary Albumin ◽  
Low Molecular Weight ◽  
Patients With Diabetes ◽  
Estimate Glomerular Filtration Rate

Background For the early identification of patients at risk of developing diabetic nephropathy, we have developed an ultrasensitive immune complex transfer enzyme immunoassay to measure adiponectin in urine. Methods We developed immune complex transfer enzyme immunoassay for adiponectin and measured urinary adiponectin from 70 healthy subjects, 35 obese non-diabetic subjects and 20 patients with diabetes. Results The urinary adiponectin concentrations in patients with diabetes (3.3 ± 10.7 ng/mg creatinine) were significantly higher than those in obese subjects (0.54 ± 0.44; P < 0.01) and healthy subjects (0.46 ± 0.42; P < 0.001). The gel filtration elution profile of urine from healthy subjects showed traces of four immunoreactive peaks (high-, medium-, low-molecular weight and monomer molecules), despite the majority of blood adiponectin being high-molecular weight. However, urinary adiponectin molecules were more frequent in low-molecular weight as the estimate glomerular filtration rate decreased. Furthermore, as blood glucose concentrations rose, middle-molecular weight and high-molecular weight increased in urine. Further, urinary adiponectin concentrations correlated with estimate glomerular filtration rate ( r = −0.61, P < 0.001), but not urinary albumin. In addition, our analysis showed a significantly ( P < 0.001) higher value for urinary adiponectin in the G2 stage of chronic kidney disease classification where urinary albumin is not elevated. Conclusion Adiponectin increases in urine as renal function decreases, and urinary adiponectin may be useful as a surrogate marker for diabetic nephropathy risk.

scholarly journals Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate

2016 ◽  
Vol 8 (26) ◽  
pp. 5190-5196 ◽  
Author(s):  
J. Yang ◽  
J. Zhu ◽  
R. Pei ◽  
J. A. Oliver ◽  
D. W. Landry ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Plasma ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Low Molecular Weight ◽  
Plasma Ultrafiltrate

We present a microfluidic aptamer-based biosensor for detection of low-molecular-weight biomarkers in patient samples.

Mass spectrometric characteristics and preliminary figures of merit for polyaromatic hydrocarbons via the liquid sampling-atmospheric pressure glow discharge microplasma

2020 ◽  
Vol 35 (11) ◽  
pp. 2475-2478
Author(s):  
Tyler J. Williams ◽  
Jacob R. Bills ◽  
R. Kenneth Marcus
Keyword(s):  
Molecular Weight ◽  
Atmospheric Pressure ◽  
Polyaromatic Hydrocarbons ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Atmospheric Pressure Glow Discharge ◽  
Figures Of Merit ◽  
Ionization Mechanisms ◽  
Pressure Glow Discharge ◽  
Polar Low

The versatility of the LS-APGD microplasma is extended beyond elemental and polar molecular species to non-polar, low molecular weight polyaromatic hydrocarbons. Insights into ionization mechanisms are gained, with preliminary LODs determined.

Biochemical Analysis of Vitreous Humor in Postmortal Diagnosis of Renal Failure

10.12737/2726 ◽  
2013 ◽  
Vol 20 (4) ◽  
pp. 47-49
Author(s):  
Терёхина ◽  
N. Terekhina ◽  
Акимов ◽  
Pavel Akimov
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Renal Failure ◽  
Serum Creatinine ◽  
Biochemical Analysis ◽  
Vitreous Humor ◽  
Control Group ◽  
Patients With Diabetes ◽  
Maximum Content

The content of medium molecular weight peptides and creatinine in serum and vitreous humor from 48 corpses of people suffering from diabetes mellitus during his lifetime and 27 without this disease, formed the control group, were investigated. The presence of renal failure among patients with diabetes mellitus and in the control group was, respectively, in 20 and 15 persons was revealed. The content of serum creatinine in cadaver blood without renal failure was more than 2-2,5 - folds in comparative with living people and was not more than 240 μηΊθΙ/L. The content of serum medium molecular weight peptides in control group was not more than 2,8 g/L, and in the group of diabetes mellitus without renal failure - 2,9 g/L. The obtained results are analogical with data of living people accordingly literature sources. Direct correlation between the content of creatinine, medium molecular weight peptides and vitreous humor and serum are estimated. The maximum content of creatinine and medium molecular weight peptides in vitreous humor were, respectively, 110 μΓηοΙ/L and 0,5 g/L. The values extreme graduated in presents of renal failure as in serum, as in vitreous humor. Thus, in postmortal period appropriate to determine the vitreous humor creatinine and medium molecular weight peptides content for the diagnosis of renal failure.

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