Gas-liquid chromatographic profile of neutral and acidic metabolites in cerebrospinal fluid from newborns and infants.

1976 ◽  
Vol 22 (5) ◽  
pp. 623-626 ◽  
Author(s):  
R D Malcolm ◽  
R Leonards
Keyword(s):  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Neurological Disorders ◽  
Trimethylsilyl Ether ◽  
Gas Liquid Chromatography ◽  
Qualitative And Quantitative ◽  
Chromatographic Pattern ◽  
Methylene Unit ◽  
Chromatographic Profile ◽  
Liquid Chromatographic

Abstract A profile (chromatographic pattern) of the neutral and acidic metabolites present in cerebrospinal fluid from newborns and infants was obtained by gas-liquid chromatography. The metabolites are those extracted with ethyl acetate and diethyl ether. They are converted to trimethylsilyl ether derivatives, and chromatographed on a column containing 5% silicone OV-101 (methyl) packed on Chromosorb W. Several substances were tentatively identified from their methylene unit values. We established a control profile for infants, and compared profiles for infants and adults. Noteworthy qualitative and quantitative differences from the control were seen for cerebrospinal fluid from subjects with neurological disorders of infections. The technique may be of use in neurological diagnosis.

Separation and Identification of Neutral and Acidic Metabolites in Cerebrospinal Fluid

1972 ◽  
Vol 18 (3) ◽  
pp. 258-262 ◽  
Author(s):  
L D Waterbury ◽  
L A Pearce
Keyword(s):  
Cerebrospinal Fluid ◽  
Trimethylsilyl Ether ◽  
Gas Liquid Chromatography ◽  
Mass Spectral Data ◽  
Drug Induced ◽  
Mass Spectral ◽  
Human Cerebrospinal Fluid ◽  
Methylene Unit ◽  
Induced Changes ◽  
Potential Use

Abstract We describe a method for obtaining profiles of neutral and acidic substances present in human cerebrospinal fluid (CSF) by gas—liquid chromatography. Metabolites extracted with ethyl acetate and ether are converted to methyl ester, trimethylsilyl ether derivatives. With this technique, acidic metabolites of brain amines, and neutral metabolites of dopamine and norepinephrine have been identified in human CSF. Some of these substances have not been reported previously in CSF. Extracted substances are identified on the basis of their methylene-unit values and mass spectral data. Potential use of this method in neurodiagnosis and in delineating drug-induced changes in CSF metabolites is discussed.

Review of Gas-Liquid Chromatography of Marihuana

1974 ◽  
Vol 57 (4) ◽  
pp. 888-892 ◽  
Author(s):  
James M Parker ◽  
Brian L Stembal
Keyword(s):  
Liquid Chromatography ◽  
Trimethylsilyl Ether ◽  
Gas Liquid Chromatography ◽  
Glass Column ◽  
Glass Columns ◽  
Liquid Chromatographic ◽  
Derivatives Of ◽  
Identification And Quantification

Abstract Gas-liquid chromatographic (GLC) methods used in the separation, identification, and quantification of Cannabis components are reviewed. Glass columns packed with 3% OV-17 offer better separation of tetrahydrocannabinol isomers and greater sensitivity. GLC identification of new Cannabis constituents, preparation of trimethylsilyl ether derivatives of known cannabinoids, and preparation of pure standards from Cannabis samples are discussed along with problems in cannabinoid quantification. Use of a 6' × ¼" glass column packed with 3% OV-17 on 100–120 mesh Gas-Chrom Q is suggested for GLC separation of Cannabis components.

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

Electron capture gas-liquid chromatographic-mass spectral identification of acids produced by Neisseria meningitidis in a defined medium

1979 ◽  
Vol 9 (1) ◽  
pp. 97-102
Author(s):  
C C Alley ◽  
J B Brooks ◽  
D S Kellogg
Keyword(s):  
Liquid Chromatography ◽  
Neisseria Meningitidis ◽  
Electron Capture ◽  
Defined Medium ◽  
Gas Liquid Chromatography ◽  
Mass Spectral ◽  
Spectral Identification ◽  
Acid Metabolites ◽  
Liquid Chromatographic ◽  
Chromatographic Mass

The acid metabolites and the cellular fatty acids of three strains of Neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. Five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (C10:1), dodecenoic (C12:1), and tetradecenoic (C14:1). These acids were produced during active metabolism and were not detected as cellular constituents. The frequency-pulsed electron capture gas-liquid chromatography methods which we used provide a rapid, reliable, sensitive means of detecting both these and other metabolic and cellular acids in spent culture medium.

Gas Chromatographic and Gas Chromatographic-Mass Spectrometric Confirmation of 2,4- and 2,6-Toluenediamine Determined by Liquid Chromatography in Aqueous Extracts

1982 ◽  
Vol 65 (6) ◽  
pp. 1388-1394 ◽  
Author(s):  
Roger C Snyder ◽  
William C Brumley ◽  
Charles V Breder ◽  
Thomas Fazio
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Mass Spectrometric ◽  
Gas Liquid Chromatography ◽  
Aqueous Extracts ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
Chromatographic Mass

Abstract The confirmation of 2,4- and 2,6-toluenediamine (TDA) in aqueous extracts from boil-in-bags and retortable pouches is described. The extracts were initially analyzed by a high performance liquid chromatographic procedure and any apparent 2,4- and/or 2,6-TDA were quantitated. The liquid chromatographic effluent corresponding to any apparent 2,4- or 2,6-TDA was collected. TDA was then partitioned into ethyl acetate and reacted with trifluoroacetic anhydride (TFAA). The TDA-TFAA derivative formed was confirmed by gas-liquid chromatography (GLC) using a 1.2 m × 0.32 cm nickel column packed with 6% OV-17 on Superpak-20M. Results obtained from analyzing extracts of several retortable pouches and boil-in-bags showed levels of TDA migration ranging from <0.1 to 2.2 ppb (μg/L). Additional confirmation of the TDA-TFAA derivative from retortable pouches by multiple ion detection GC/mass spectrometry is also described.

Gas-Liquid Chromatographic Determination of Sodium Fluoroacetate (Compound 1080)

1980 ◽  
Vol 63 (1) ◽  
pp. 49-55
Author(s):  
Iwao Okuno ◽  
Dennis L Meeker
Keyword(s):  
Liquid Chromatography ◽  
Tissue Sample ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Tissue Samples ◽  
Fluoroacetic Acid ◽  
Pentafluorobenzyl Bromide ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract An analytical method is described for the determination of Compound 1080 (sodium fluoroacetate) residues in 1–10 g tissue. Sample extracts of tissues are cleaned up with silica gel, and Compound 1080 (as fluoroacetic acid) is separated by a micro-distillation procedure. The fluoroacetic acid in the distillate is derivatized with pentafluorobenzyl bromide to form pentafluorobenzyl fluoroacetate which is measured by electron capture gas-liquid chromatography. Recoveries of sodium fluoroacetate from fortified tissue samples averaged about 25%. Despite the limited recoveries, results were quite reproducible, and levels as low at 2 ppm were determined in fortified 1 g samples, and 0.2 ppm in 10 g samples. The method is relatively simple and has been used routinely in our laboratory for the analysis of various types of samples such as grain, and tissues from birds, rodents, and larger animals.

Gas-Liquid Chromatographic Determination of Maleic Hydrazide in Tobacco and Tobacco Smoke

1979 ◽  
Vol 62 (1) ◽  
pp. 171-175 ◽  
Author(s):  
Alfred F Haeberer ◽  
Orestes T Chortyk
Keyword(s):  
Liquid Chromatography ◽  
Tobacco Smoke ◽  
Plant Growth Regulator ◽  
Maleic Hydrazide ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Trimethylsilyl Derivative ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is presented for the determination of the plant growth regulator maleic hydrazide (MH; l,2-dihydro-3,6-pyridazinedione) in tobacco and tobacco smoke. Residues are converted to the bis(trimethylsilyl) derivative before analysis by gas-liquid chromatography. The method has been applied to cigarettes and condensed smoke and has been used to determine the per cent transfer of MH into cigarette smoke. Free MH residues could be determined directly on the tobacco samples, whereas total MH values were obtainable only after acid hydrolysis. In spite of large MH residues in tobacco, only 0.2% of the MH was transferred into smoke.

Measurement of thiamylal and thiopental in plasma by electron capture and flame photometric gas-liquid chromatography.

1977 ◽  
Vol 23 (7) ◽  
pp. 1306-1309 ◽  
Author(s):  
R H Smith ◽  
J A MacDonald ◽  
D S Thompson ◽  
W E Flacke
Keyword(s):  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Electron Capture ◽  
Internal Standard ◽  
Chromatographic Column ◽  
Gas Liquid Chromatography ◽  
Unchanged Drug ◽  
Chromatographic Procedure ◽  
Isothermal Gas ◽  
Liquid Chromatographic

Abstract We describe an isothermal gas-liquid chromatographic procedure developed for measuring thiamylal and thiopental in plasma. The unchanged drug is extracted into ethyl acetate from acidified plasma, together with an internal standard. The solvent is removed, the residue methylated, aliquots, diluted with benzene, are injected into a 183-cm gas-liquid chromatographic column containing 3% OV-17. Sensitivity of detection is in the nanogram to picogram range.

Gas--liquid chromatographic microdetermination of underivatized ethosuximide (alpha-ethyl-alpha-methyl succinimide) in plasma or serum.

1978 ◽  
Vol 24 (10) ◽  
pp. 1821-1823 ◽  
Author(s):  
A J Fellenberg ◽  
A C Pollard
Keyword(s):  
Liquid Chromatography ◽  
Detection Threshold ◽  
Internal Standard ◽  
Chloroform Extract ◽  
Gas Liquid Chromatography ◽  
Clinical Use ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic procedure for the microdetermination of ethosuximide is described. Ethosuximide is extracted from acidified plasma or serum into chloroform containing an internal standard alpha,alpha-dimethyl-beta-methyl succinimide. Part of the initial chloroform extract is gently evaporated, the residue redissolved in n-heptane at 60 degrees C, and an aliquot analyzed by gas-liquid chromatography. The procedure is rapid, reliable, sensitive, and specific. It requires a 25--50 microliter sample for a single estimation, has a detection threshold of less than 10 micromol/liter, and is suitable for routine clinical use.

Sign in / Sign up

Export Citation Format

Share Document