Creatine kinase isoenzyme MM variants in skeletal muscle and plasma from marathon runners.

1986 ◽  
Vol 32 (1) ◽  
pp. 41-44 ◽  
Author(s):  
F S Apple ◽  
M A Rogers ◽  
J L Ivy
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Strenuous Exercise ◽  
Variant Form ◽  
Distance Runners ◽  
Long Distance ◽  
Marathon Runners ◽  
Creatine Kinase Isoenzyme ◽  
Before And After

Abstract We investigated the patterns of variants of creatine kinase isoenzyme MM (CK-MM) in gastrocnemius muscle and plasma sampled from male and female long-distance runners before and after a marathon race. The proportions of CK-MM variants MM1 (pI 6.90) and MM2 (pI 6.62), identified in the skeletal muscle from both sexes, did not differ significantly from those in skeletal muscle from nonrunning controls or from heart muscle. CK-MM1 was the major (84-85% of total CK-MM) variant form. Patterns of CK-MM in plasma collected from male runners 24, 48, 72, and 96 h after the race were similar to those for female runners, but we detected two new additional variants, which we designate MM1B (pI 6.76) and MM2B (pI 6.49). For both sexes the total CK-MM activities in plasma were significantly (p less than 0.05) greater after the race, but the women's total CK-MM activities were significantly (p less than 0.05) less than the men's. The rates of disappearance of MM1, MM2, and MM3 from plasma after the race differed significantly (p less than 0.05) between men and women, MM1 clearing the fastest. Determination of the CK-MM variants in plasma after strenuous exercise may be of help in assessing CK release from injured skeletal muscle.

Profile of creatine kinase isoenzymes in skeletal muscles of marathon runners.

1984 ◽  
Vol 30 (3) ◽  
pp. 413-416 ◽  
Author(s):  
F S Apple ◽  
M A Rogers ◽  
W M Sherman ◽  
D L Costill ◽  
F C Hagerman ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Skeletal Muscles ◽  
Gastrocnemius Muscle ◽  
Distance Runners ◽  
Long Distance ◽  
Marathon Runners ◽  
Isoenzyme Composition ◽  
The Mean ◽  
Muscle Biopsies

Abstract The proportion of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB activity was increased in skeletal muscle biopsies obtained from five long-distance runners, both 2 h before (mean 7.7%, SD 2.4%) and 30 min after (mean 7.2%, SD 1.2%) a marathon race, as compared with that in biopsies from five nonrunners (controls less than or equal to 1.0%). Further, mitochondrial CK and CK-BB isoenzymes were present in homogenates of the runners' skeletal muscle samples but not in those of the nonrunners. However, there were no substantial differences in the mean total CK activities per gram (wet wt.) of muscle tissue among premarathon samples, postmarathon samples, and nonrunners' samples (3148, 3365, and 3049 U/g, respectively). We conclude that the metabolically active gastrocnemius muscle of long-distance runners is qualitatively similar to the heart muscle in its CK isoenzyme composition.

Creatine kinase-MB isoenzyme adaptations in stressed human skeletal muscle of marathon runners

1985 ◽  
Vol 59 (1) ◽  
pp. 149-153 ◽  
Author(s):  
F. S. Apple ◽  
M. A. Rogers ◽  
D. C. Casal ◽  
W. M. Sherman ◽  
J. L. Ivy
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Gastrocnemius Muscle ◽  
Acute Exercise ◽  
Elevated Serum ◽  
Long Distance ◽  
Marathon Runners ◽  
Creatine Kinase Mb ◽  
Isoenzyme Composition ◽  
Muscle Biopsies

The creatine kinase (CK) isoenzyme composition was determined in serial gastrocnemius muscle biopsies obtained from 12 male marathon runners. The mean muscle CK-MB composition significantly increased after chronic exercise (training) from 5.3% (pretraining) to 7.7% (premarathon) as well as after acute exercise (postmarathon) to 10.5% of the total CK activity (P less than 0.05). However, no significant differences in total CK activities were detected. Additionally, mitochondrial CK and CK-BB isoenzymes were present in muscle homogenates. A significant correlation was observed in the increase in mean serum total CK (3,322 U/l) and CK-MB (174 U/l) activities 24 h after the race (r = 0.98, P less than 0.05). These results show that gastrocnemius muscle adapts to long-distance training and racing with increased CK-MB activities and imply that skeletal muscle is the major source of elevated serum CK-MB activities in marathon runners.

Clinical evaluation of an immunoinhibition procedure for creatine kinase-MB.

1980 ◽  
Vol 26 (1) ◽  
pp. 150-152
Author(s):  
D Obzansky ◽  
J A Lott
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Diagnostic Sensitivity And Specificity ◽  
Creatine Kinase Isoenzyme Mb

Abstract We have clinically evaluated the Dade "Cardiozyme" immunoinhibition procedure for determination of creatine kinase isoenzyme MB (CK-MG) in 71 patients who were suspected of having had an acute myocardial infarction. Electrophoresis for CK-MB was also carried out. On the basis of diagnostic sensitivity and specificity for myocardial infarction, we found the Dade procedure for CK-MB to be somewhat inferior to electrophoresis. In 11 patients for whom the time of infarction was known, we observed normal CK-MB results for two of them by both immunoinhibition and electrophoresis during the first 24 h, but subsequently could detect abnormal CK-MB results by both methods. Thus in some patients such data are not helpful for making a diagnosis in the first 24 h. The Dade procedure is easy to perform, but lacks sensitivity in the region of low CK-MB activity, requires a very stable spectrophotometer, is imprecise, and produces negative numerical results in patients without myocardial infarction.

Ratio of Creatine Kinase 2 Mass Concentration to Total Creatine Kinase Activity not Altered by Heavy Physical Exercise

1992 ◽  
Vol 38 (11) ◽  
pp. 2224-2227 ◽  
Author(s):  
J Ordóñez-Llanos ◽  
J R Serra-Grima ◽  
J Mercé-Muntañola ◽  
F González-Sastre
Keyword(s):  
Creatine Kinase ◽  
Mass Concentration ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Marathon Runners ◽  
Creatine Kinase Isoenzyme ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Sedentary Subjects

Abstract Serum creatine kinase isoenzyme 2 concentrations (CK 2 mass) were measured in marathon runners during training and 1 and 2 days after a race and compared with values from 36 acute myocardial infarction (AMI) patients whose total CK and (or) CK 2 activities were similar to those of runners in the basal state. During training, runners had CK and CK 2 activities 53% and 43% above reference values, respectively, and 36% had CK 2 activity > 5% of total CK. Nine runners (26%) showed CK 2 mass values > 6 micrograms/L but < or = 10 micrograms/L; 35 of the AMI subjects, despite having CK activities similar to those of runners, had values > 10 micrograms/L. The ratio of CK 2 mass to total CK activity was significantly (P < 0.0002) different between sexes for runners. At 1 and 2 days after racing, 100% of CK and CK 2 activities and 71% and 57% of the percentages of CK 2 activity, respectively, were abnormally high; 57% and 43% of CK 2 mass values were > 10 micrograms/L, being comparable with those observed for the AMI group. Basal CK 2 mass values of the runners appeared only slightly higher than that for sedentary subjects, but after exercise half the subjects presented increased values similar to those observed for AMI subjects. The ratio of CK 2 mass to total CK activity appeared unaltered by exercise in all but one of the samples assayed, indicating its utility in evaluating CK 2 mass increases originating in skeletal muscle.

scholarly journals Relationship between skeletal muscle fiber properties and O_2 debt capacity in long-distance runners

1991 ◽  
Vol 36 (2) ◽  
pp. 177-183
Author(s):  
Ryoichi Mitsuzono ◽  
Shigeru Katsuta ◽  
Yoji Kanao ◽  
Hirofumi Miyata ◽  
Kazunori Asaba ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Distance Runners ◽  
Long Distance ◽  
Fiber Properties ◽  
Debt Capacity

scholarly journals Cryotherapy Models and Timing-Sequence Recovery of Exercise-Induced Muscle Damage in Middle- and Long-Distance Runners

2020 ◽  
Vol 55 (4) ◽  
pp. 329-335
Author(s):  
Chaoyi Qu ◽  
Zhaozhao Wu ◽  
Minxiao Xu ◽  
Fei Qin ◽  
Yanan Dong ◽  
...  
Keyword(s):  
Muscle Damage ◽  
Cold Water ◽  
Water Immersion ◽  
Whole Body ◽  
Strenuous Exercise ◽  
Distance Runners ◽  
Long Distance ◽  
Positive Effects ◽  
Exercise Induced ◽  
Recovery Methods

Context Among sports-recovery methods, cold-water immersion (CWI), contrast-water therapy (CWT), and whole-body cryotherapy (WBC) have been applied widely to enhance recovery after strenuous exercise. However, the different timing effects in exercise-induced muscle damage (EIMD) after these recovery protocols remain unknown. Objective To compare the effects of CWI, CWT, and WBC on the timing-sequence recovery of EIMD through different indicator responses. Design Crossover study. Setting Laboratory. Patients or Other Participants Twelve male middle- and long-distance runners from the Beijing Sport University (age = 21.00 ± 0.95 years). Intervention(s) Participants were treated with different recovery methods (control [CON], CWI, CWT, WBC) immediately postexercise and at 24, 48, and 72 hours postexercise. Main Outcome Measure(s) We measured perceived sensation using a visual analog scale (VAS), plasma creatine kinase (CK) activity, plasma C-reactive protein (CRP) activity, and vertical-jump height (VJH) pre-exercise, immediately postexercise, and at 1, 24, 48, 72, and 96 hours postexercise. Results For the VAS score and CK activity, WBC exhibited better timing-sequence recovery effects than CON and CWI (P < .05), but the CWT demonstrated better effects than CON (P < .05). The CRP activity was lower after WBC than after the other interventions (P < .05). The VJH was lower after WBC than after CON and CWI (P < .05). Conclusions The WBC positively affected VAS, CK, CRP, and VJH associated with EIMD. The CWT and CWI also showed positive effects. However, for the activity and timing-sequence effect, CWT had weaker effects than WBC.

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