Effect of red wine ingestion on the antioxidant capacity of serum

Abstract Aerobic metabolism in biological systems produces reactive oxygen species, and defense against such prooxidants requires antioxidant activity, e.g., predominantly vitamins C and E in serum. Recently, flavonoids (polyphenols occurring widely in plants) have been investigated in vitro for their antioxidant activity; whether they are absorbed after ingestion is not clear. Using a chemiluminescent assay of serum antioxidant capacity (SAOC), we have studied the effects in normal individuals of ingesting red wine, white wine, and high doses of vitamin C. In nine subjects who ingested 300 mL of red wine, the mean SAOC was increased by 18% after 1 h and by 11% at 2 h. The same amount of white wine produced 4% and 7% increases, respectively. The ingestion of 1000 mg (5.7 mmol) of ascorbic acid by four subjects increased their mean SAOC by 22% at 1 h and by 29% at 2 h. An in vitro comparison of red wine, white wine, and various fruit juices showed the high antioxidant capacity of red wine in addition to its ability to increase the antioxidant capacity of serum in vivo. The antioxidant effects of various flavonoids and other polyphenols were also studied.

Download Full-text

Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofSudarshanaPowder

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/6743862 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Weerakoon Achchige Selvi Saroja Weerakoon ◽

Pathirage Kamal Perera ◽

Dulani Gunasekera ◽

Thusharie Sugandhika Suresh

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Thiobarbituric Acid ◽

Trolox Equivalent Antioxidant Capacity ◽

Control Group ◽

Thiobarbituric Acid Reactive Substance ◽

Trolox Equivalent ◽

Antioxidant Effects

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

Download Full-text

Phytochemical Analysis of Anti-Inflammatory and Antioxidant Effects of Mahonia aquifolium Flower and Fruit Extracts

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/2879793 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 13

Author(s):

Andra-Diana Andreicut ◽

Alina Elena Pârvu ◽

Augustin Cătălin Mot ◽

Marcel Pârvu ◽

Eva Fischer Fodor ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Stress Index ◽

Tnf Alpha ◽

Phytochemical Analysis ◽

Anti Inflammatory ◽

Mahonia Aquifolium ◽

Antioxidant Effects

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.

Download Full-text

Total polyphenol content and antioxidant activity of commercial Noni (Morinda citrifolia L.) juice and its components

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502010000400006 ◽

2010 ◽

Vol 46 (4) ◽

pp. 651-656 ◽

Cited By ~ 6

Author(s):

Adriana Bramorski ◽

Adriana da Rosa Cherem ◽

Chaiana Paula Marmentini ◽

Joseane Torresani ◽

Tatiana Mezadri ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Grape Juice ◽

Morinda Citrifolia ◽

Polyphenol Content ◽

Total Polyphenol ◽

Total Polyphenol Content ◽

Treatment And Prevention

The plant Morinda citrifolia L. (noni) has been the focus of many recent studies due to its potential effects on treatment and prevention of several diseases. However, there are few in vivo and in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine the total polyphenol content (TPC) and antioxidant capacity of a juice commercialized as noni juice, but containing grape, blueberry and noni fruits. Commercial noni juice was compared against its separate constituents of blueberry and grape juice. Folin-Ciocalteu and DPPH• methods were used to determine the concentration of total polyphenol content and antioxidant activity, respectively. Commercial noni juice presented higher values of TPC (91.90 mg of gallic acid/100 mL juice) and antioxidant activity (5.85 mmol/L) compared to its 5% diluted constituents. Concentrated blueberry juice presented higher TPC and antioxidant activity than the other juices analyzed. Considering that the blueberry and grape juices account for only 10% in the composition of commercial noni juice, it can be inferred that these two components contribute significantly to the antioxidant activity. Therefore, additional studies are necessary in order to elucidate the contribution of the noni juice as an antioxidant.

Download Full-text

Heparin Induced Release of Low Affinity Platelet Factor Four

10.1055/s-0039-1684471 ◽

1979 ◽

Author(s):

A.K. Rao ◽

P. James ◽

S. Niewiarowski

Keyword(s):

Important Implication ◽

Specific Protein ◽

Final Concentration ◽

Antigenic Determinants ◽

Platelet Factor ◽

Normal Individuals ◽

The Mean ◽

Minute Sample

Heparin has been reported to effect platelet function. We studied the effect of commercial beef lung heparin on the release of a platelet specific protein, the low affinity platelet factor 4(LA-PF4) in vitro and vivo. LA-PF4 shares Common antigenic determinants with B thromboglobulin, LA-PF4 was measured in platelet poor plasma (PPP) by radioimmunoassay. The mean levels of LA-PF4 in PPP from citrated blood and citrated blood with added heparin (final concentration 1.5 unit/ml) were 78.3±18.3 and 242.5±117.1 ng/ml respectively. (Mean ± SD, p <0.01). Addition of heparin to blood collected in a mixture of E EDTA, prostaglandin El, and theophylline did not result in an increase in LA-PF4 in PPP. A single intravenous bolus of heparin was administered to two normal individuals and the levels of LA-PF4 measured before and at 15,30 and 180 minutes later. An increase in the levels of LA-PF4 in the PPP from a baseline of 66.7 and 30.2 ng/ml/106 platelets to 166.7 and 167.4 ng/ml/106 platelets respectively were noted in 30 minute sample. There was no increase in LA-PF4 at 15 minutes. At 180 minutes levels were at baseline. It is concluded that heparin stimulates release of LA-PF4 from platelets both in vitro and in vivo. LA-PF4 has antiheparin action and is being investigated as a marker for thrombosis. Its release by heparin may, therefore, have an important implication.

Download Full-text

Callus Culture of Thai Basil Is an Effective Biological System for the Production of Antioxidants

Molecules ◽

10.3390/molecules25204859 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4859 ◽

Cited By ~ 1

Author(s):

Saher Nazir ◽

Hasnain Jan ◽

Duangjai Tungmunnithum ◽

Samantha Drouet ◽

Muhammad Zia ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Callus Culture ◽

Biological System ◽

Rosmarinic Acid ◽

Callus Cultures ◽

Trolox Equivalent Antioxidant Capacity ◽

Chicoric Acid

Thai basil is a renowned medicinal plant and a rich source of bioactive antioxidant compounds with several health benefits, with actions to prevent of cancer, diabetes and cardiovascular disease. Plant cell and tissue culture technologies can be routinely established as an important, sustainable and low-cost biomass source to produce high-value phytochemicals. The current study aimed at developing an effective protocol to produce Thai basil leaf-derived callus cultures with sustainable and high production of biomass and antioxidants as an alternative of leaves production. MS basal medium with various concentrations of plant growth regulators (PGRs) compatible with nutraceutical applications (i.e., gibberellic acid (GA3) and 6-benzylaminopurine (BAP) either alone or in combination with naphthalene acetic acid (NAA)) were evaluated. Among all tested PGRs, the combination BAP:NAA (5 mg/L:1 mg/L) yields the maximum biomass accumulation (fresh weight (FW): 190 g/L and dry weight (DW): 13.05 g/L) as well as enhanced phenolic (346.08 mg/L) production. HPLC quantification analysis indicated high productions of chicoric acid (35.77 mg/g DW) and rosmarinic acid (7.35 mg/g DW) under optimized callus culture conditions. Antioxidant potential was assessed using both in vitro cell free and in vivo cellular antioxidant assays. Maximum in vitro antioxidant activity DPPH (93.2% of radical scavenging activity) and ABTS (1322 µM Trolox equivalent antioxidant capacity) was also observed for the extracts from callus cultures grown in optimal conditions. In vivo cellular antioxidant activity assay confirmed the effective protection against oxidative stress of the corresponding extract by the maximum inhibition of ROS and RNS production. Compared to commercial leaves, callus extracts showed higher production of chicoric acid and rosmarinic acid associated with higher antioxidant capacity. In addition, this biological system also has a large capacity for continuous biomass production, thus demonstrating its high potential for possible nutraceutical applications.

Download Full-text

Antinociceptive Activity and Redox Profile of the Monoterpenes (+)-Camphene, p-Cymene, and Geranyl Acetate in Experimental Models

ISRN Toxicology ◽

10.1155/2013/459530 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 34

Author(s):

Lucindo Quintans-Júnior ◽

José C. F. Moreira ◽

Matheus A. B. Pasquali ◽

Soheyla M. S. Rabie ◽

André S. Pires ◽

...

Keyword(s):

Free Radical ◽

Antinociceptive Effect ◽

Experimental Models ◽

Geranyl Acetate ◽

No Production ◽

Significant Activity ◽

High Doses ◽

Antioxidant Effects

Objective. To evaluate antinocicpetive and redox properties of the monoterpenes (+)-camphene, p-cymene, and geranyl acetate in in vivo and in vitro experimental models. Methods. Evaluation of the in vitro antioxidant activity of (+)-camphene, p-cymene, and geranyl acetate using different free radical-generating systems and evaluation of antinociceptive actions by acetic acid-induced writhing and formalin-induced nociception tests in mice. Results. p-Cymene has the strongest antinociceptive effect, but (+)-camphene and geranyl acetate also present significant activity at high doses (200 mg/kg). (+)-Camphene had the strongest antioxidant effect in vitro at TBARS and TRAP/TAR assays and also had the highest scavenging activities against different free radicals, such as hydroxyl and superoxide radicals. Sodium nitroprussiate-derived NO production was enhanced by (+)-camphene. Geranyl acetate and p-cymene also presented some antioxidant effects, but with a varying profile according the free radical-generating system studied. Conclusion. (+)-Camphene, p-cymene, and geranyl acetate may present pharmacological properties related to inflammation and pain-related processes, being potentially useful for development of new therapeutic strategies, with limited possibilities for p-cymene and geranyl acetate.

Download Full-text

Rutin-Loaded Nanovesicles for Improved Stability and Enhanced Topical Efficacy of Natural Compound

Journal of Functional Biomaterials ◽

10.3390/jfb12040074 ◽

2021 ◽

Vol 12 (4) ◽

pp. 74

Author(s):

Maria Chiara Cristiano ◽

Antonella Barone ◽

Antonia Mancuso ◽

Daniele Torella ◽

Donatella Paolino

Keyword(s):

Antioxidant Activity ◽

Encapsulation Efficiency ◽

Natural Compound ◽

Pharmacological Effects ◽

Great Stability ◽

Improved Stability ◽

Characterization Studies ◽

Antioxidant Effects

Rutin is a natural compound with several pharmacological effects. Among these, antioxidant activity is one of the best known. Despite its numerous benefits, its topical application is severely limited by its physicochemical properties. For this reason, the use of suitable systems could be necessary to improve its delivery through skin, thus enhancing its pharmacological effects. In this regard, the aim of this work is to optimize the ethosomal dispersion modifying both lipid and ethanol concentrations and encapsulating different amounts of rutin. Characterization studies performed on the realized systems highlighted their great stability properties. Studies of encapsulation efficiency and loading degree allowed us to identify a better formulation (EE% 67.5 ± 5.2%, DL% 27 ± 1.7%), which was used for further analyses. The data recorded from in vitro studies showed that the encapsulation into these nanosystems allowed us to overcome the photosensitivity limitation of rutin. Indeed, a markable photostability of the loaded formulation was recorded, compared with that reported from the free rutin solution. The efficacy of the nanosystems was finally evaluated both in vitro on keratinocyte cells and in vivo on human healthy volunteers. The results confirmed the potentiality of rutin-loaded nanosystems for skin disease, mainly related to their anti-inflammatory and antioxidant effects.

Download Full-text

A REVIEW ON ANTIOXIDANT METHODS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13092 ◽

2016 ◽

pp. 14 ◽

Cited By ~ 5

Author(s):

Dontha Sunitha

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Free Radical ◽

Oxygen Species ◽

Antioxidant Assay ◽

Antioxidant Effects ◽

In Vitro Antioxidant Activity

ABSTRACT To provide an outlook of the various available methods of antioxidant activity. Various available in vitro and in vivo methods are listed and the procedure to perform the method, its mechanism is also explained in brief. 1,1-diphenyl-2-picrylhydrazyl method was found to be used mostly for the in vitro antioxidant activity evaluation purpose while lipid peroxidation was found as mostly used in vivo antioxidant assay. An ethanol was with the highest frequency as a solvent for extraction purpose. Summarized information on the various methods available provides with reliable information to confirm the benefits of antioxidant effects. Keywords: Antioxidant activity, Reactive oxygen species, Free radical, 1,1-diphenyl-2-picrylhydrazyl, Flavonoid.

Download Full-text

Phenolic Composition, Antioxidant Activity, andIn VitroAvailability of Four Different Berries

Journal of Chemistry ◽

10.1155/2016/5194901 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 22

Author(s):

Javier Marhuenda ◽

María Dolores Alemán ◽

Amadeo Gironés-Vilaplana ◽

Alfonso Pérez ◽

Gabriel Caravaca ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Antioxidant Capacity ◽

Total Phenolic ◽

Phenolic Composition ◽

Total Phenolic Compounds ◽

Anthocyanin Concentration

Polyphenols from berries have proved healthy effects after“in vitro”and“in vivo”studies, such as preventing tumor growing and neurodegenerative and cardiovascular diseases. We compared four different kinds of berries—strawberry, raspberry, blackberry, and blueberry—with the aim to distinguish their phenolic composition, concerning their antioxidant capacity along with their“in vitro”availability. Folin-Ciocalteu method was used for the determination of phenolic compounds, and the antioxidant capacity was measured by ORAC method. Moreover, the determination of anthocyanins was accomplished with an HPLC-DAD. Finally, we carried out an“in vitro”digestion to simulate the gastrointestinal digestion. All berries showed good antioxidant capacity with significant differences, besides high total phenolic compounds. Content of anthocyanins measured by HPLC-DAD varied between the different berries, namely, blackberries and strawberries which showed higher anthocyanin concentration. After“in vitro”digestion, berries showed poor bioavailability of the analysis of anthocyanins (9.9%–31.7%). Availability of total phenolic compounds was higher than anthocyanins (33%–73%). Moreover, strawberries and blackberries presented the less availability grade. Decrease in antioxidant activity measured by ORAC method was about 90% in all berries studied. Therefore, bioavailability of phenolic compounds remains unclear and more correlation between“in vitro”and“in vivo”studies seems to be necessary.

Download Full-text