Inhibitory effect of black cumin (Nigella sativa) seed essential oil on Fusobacterium nucleatum L-methionine-γ-lyase (L-methioninase) activity

2021 ◽  
Author(s):  
Masao Ishikawa ◽  
Takatoshi Murata ◽  
Masaaki Okamoto ◽  
Mayu Miyanohara ◽  
Mamiko Yamashita ◽  
...  
Keyword(s):  
Essential Oil ◽  
Nigella Sativa ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Methyl Mercaptan ◽  
Kinetic Assay ◽  
Black Cumin ◽  
Wide Range ◽  
Lyase Activity ◽  
Deodorizing Activity

Abstract The enzyme L-methionine-γ-lyase is commonly found in a wide range of bacteria and catalyzes the α-elimination and γ-elimination of L-methionine to produce methyl mercaptan, α-ketobutyrate, and ammonia. Black cumin seed essential oil (BC oil) reportedly exhibits deodorizing activity against methyl mercaptan. Therefore, we hypothesized that BC oil may also suppress methyl mercaptan production. In this study, we aimed to evaluate the inhibitory effect of BC oil on L-methionine-γ-lyase activity in Fusobacterium nucleatum. Recombinant L-methionine-γ-lyase was incubated under appropriate conditions with BC oil and its constituent thymoquinone. To analyze L-methionine-γ-lyase activity, α-ketobutyric acid and ammonia concentrations were determined. The concentrations of α-ketobutyric acid and ammonia were significantly decreased by 10 µg mL−1 of BC oil (P < 0.01) and 16.4 µg mL−1 of thymoquinone (P < 0.05). An enzyme kinetic assay showed a mixed inhibition pattern between L-methionine-γ-lyase and thymoquinone. In conclusion, BC oil not only had a deodorizing effect against methyl mercaptan but also an inhibitory effect on methyl mercaptan production through the suppression of L-methionine-γ-lyase activity. Thymoquinone may be mainly responsible for these effects of BC oil. Thus, application of natural BC oil may be adapted not only for medical use but also in other areas of life.

scholarly journals Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 43-51 ◽  
Author(s):  
R. Kabiri ◽  
F. Nasibi ◽  
H. Farahbakhsh
Keyword(s):  
Salicylic Acid ◽  
Drought Stress ◽  
Nigella Sativa ◽  
Soluble Sugar ◽  
Hydroponic Culture ◽  
Black Cumin ◽  
Lyase Activity ◽  
Four Levels ◽  
Phenylalanine Ammonia Lyase Activity ◽  
Experimental Treatments

To study the effect of salicylic acid on photosynthetic pigments (chlorophyll a, b, total chlorophyll, and carotenoids), polyphenol compounds, anthocyanin, flavonoids, phenylalanine ammonia-lyase activity, malondialdehyde, lipoxygenase activity, electrolyte leakage, relative water content, soluble sugar contents, and protein content of black cumin (Nigella sativa) under drought stress in hydroponic culture, an experiment was conducted as a completely randomised design in a factorial arrangement with three replicates. Experimental treatments included salicylic acid at three levels (0, 5, and 10µM) and drought stress (induced by polyethylene glycol 6000) at four levels (0, –0.2, –0.4, and –0.6 MPa). Results showed that salicylic acid application through the root medium increased drought tolerance of black cumin seedlings. Plants pre-treated with salicylic acid exhibited slight injury symptoms whereas those not pre-treated with salicylic acid had moderate damage and lost considerable portions of their foliage. In conclusion, salicylic acid could protect the Nigella plant against drought stress through increasing of all the mentioned traits, and 10µM salicylic acid was the most effective level under both conditions.

scholarly journals Nigella sativaFixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin-Induced Diabetes Mellitus

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Muhammad Tauseef Sultan ◽  
Masood Sadiq Butt ◽  
Roselina Karim ◽  
M. Zia-Ul-Haq ◽  
Rizwana Batool ◽  
...  
Keyword(s):  
Public Health ◽  
Diabetes Mellitus ◽  
Free Radicals ◽  
Essential Oil ◽  
Nigella Sativa ◽  
Conjugated Dienes ◽  
Entire Study ◽  
Traditional Medicinal Plants ◽  
Wide Range ◽  
Streptozotocin Induced Diabetes

In the recent era, diabetes mellitus has emerged as one of the significant threats to public health and this situation demands the attention of the researchers and allied stakeholders. Dietary regimens using functional and nutraceutical foods are gaining wide range of acceptance and some traditional medicinal plants are of considerable importance. The main objective of this instant study was to explore the antidiabetic potential ofNigella sativafixed oil (NSFO) and essential oil (NSEO). Three experimental groups of rats received diets during the entire study duration, that is, D1(control), D2(NSFO: 4.0%), and D3(NSEO: 0.30%). Experimental diets (NSFO & NSEO) modulated the lipid profile, while decreasing the antioxidant damage. However, production of free radicals, that is, MDA, and conjugated dienes increased by 59.00 and 33.63%, respectively, in control. On the contrary, NSFO and NSEO reduced the MDA levels by 11.54 and 26.86% and the conjugated dienes levels by 32.53 and 38.39%, respectively.N. sativaoils improved the health and showed some promising anti-diabetic results.

Morphological and yield related traits, essential oil and oil production of different landraces of black cumin (Nigella sativa) in Iran

2018 ◽  
Vol 233 ◽  
pp. 1-8 ◽  
Author(s):  
Seyed Sajjad Hosseini ◽  
Farsad Nadjafi ◽  
Mohammad Hassan Asareh ◽  
Hassan Rezadoost
Keyword(s):  
Essential Oil ◽  
Nigella Sativa ◽  
Oil Production ◽  
Black Cumin

scholarly journals Deodorant Activity of Black Cumin Seed Essential Oil against Garlic Organosulfur Compound

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1874
Author(s):  
Yuri Yoshioka ◽  
Shinichi Matsumura ◽  
Masanori Morimoto
Keyword(s):  
Essential Oil ◽  
Nigella Sativa ◽  
Organosulfur Compound ◽  
Major Constituent ◽  
Organosulfur Compounds ◽  
Black Cumin ◽  
Allyl Mercaptan ◽  
Black Cumin Seed ◽  
Cumin Seed

The deodorant activity of black cumin (Nigella sativa L.) seed, a spice used to flavor curry and vegetable foods in Southwest Asia, against garlic (Allium sativum L.) organosulfur compounds related to human malodor was evaluated. Black cumin seed essential oil showed remarkable deodorant activity against garlic essential oil. The mode of action of this deodorant activity was presumed to be that black cumin seed essential oil covalently reacted with the organosulfur compounds in garlic. Therefore, thymoquinone, which is a major constituent in black cumin seed essential oil, and allyl mercaptan, which is one of the organosulfur compounds produced by cutting garlic, were reacted in vitro, and the products were purified and elucidated using spectroscopic data. As a result, these substances were identified as different allyl mercaptan adducts to dihydrothymoquinone. This chemical reaction was presumed to play a key role in the deodorant activity of black cumin seed essential oil.

scholarly journals Chemical Composition, Antimicrobial, Antioxidant, and Antiproliferative Properties of Grapefruit Essential Oil Prepared by Molecular Distillation

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 217 ◽  
Author(s):  
Weihui Deng ◽  
Ke Liu ◽  
Shan Cao ◽  
Jingyu Sun ◽  
Balian Zhong ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cancer Cells ◽  
Molecular Distillation ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Cell Counting ◽  
Diffusion Method ◽  
Light Phase ◽  
Wide Range

Grapefruit essential oil has been proven to have wide range of bioactivities. However, bioactivity of its molecular distillate has not been well studied. In this study, a light phase oil was obtained by molecular distillation from cold-pressed grapefruit essential oil and GC-MS was used to identify its chemical composition. The antimicrobial activity of the light phase oil was tested by filter paper diffusion method, and the anticancer activity was determined by the Cell Counting Kit-8 (CCK-8) assay. Twenty-four components were detected with a total relative content of 99.74%, including 97.48% of terpenes and 1.66% of oxygenated terpenes. The light phase oil had the best antimicrobial effect on Bacillus subtilis, followed by Escherichia coli, Staphylococcus aureus and Salmonellaty phimurium. DPPH and ABTS assays demonstrated that the light phase oil had good antioxidant activity. The CCK-8 assay of cell proliferation showed that the light phase oil had a good inhibitory effect on the proliferation of HepG2 liver cancer cells and HCT116 colon cancer cells.

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