Concurrent Infection With Multiple Human Papillomavirus Types Among Unvaccinated and Vaccinated 17-Year-Old Norwegian Girls

Abstract Background Whether type-specific human papillomavirus (HPV) infection influences the risk of acquiring infections with other HPV types is unclear. We studied concurrent HPV infections in 17-year-old girls from 2 birth cohorts; the first vaccine-eligible cohort in Norway and a prevaccination cohort. Methods Urine samples were collected and tested for 37 HPV genotypes. This study was restricted to unvaccinated girls from the prevaccination cohort (n = 5245) and vaccinated girls from the vaccine-eligible cohort (n = 4904). Risk of HPV infection was modelled using mixed-effect logistic regression. Expected frequencies of concurrent infection with each pairwise combination of the vaccine types and high-risk types (6/11/16/18/31/33/35/39/45/51/52/56/58/59) were compared to observed frequencies. Results Infection with multiple HPV types was more common among unvaccinated girls than vaccinated girls (9.2% vs 3.7%). HPV33 and HPV51 was the only HPV pair that was detected together more often than expected among both unvaccinated (P = .002) and vaccinated girls (P < .001). No HPV pairs were observed significantly less often than expected. Conclusions HPV33 and HPV51 tended to be involved in coinfection among both unvaccinated and vaccinated girls. The introduction of HPV vaccination does not seem to have had an effect on the tendency of specific HPV types to cluster together.

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Anal human papillomavirus infection and its relations with abnormal anal cytology among MSM with or without HIV infection in Japan

10.21203/rs.3.rs-232653/v1 ◽

2021 ◽

Author(s):

Daisuke Shiojiri ◽

Daisuke Mizushima ◽

Misao Takano ◽

Koji Watanabe ◽

Naokatsu Ando ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Hiv Infection ◽

Hpv Vaccination ◽

Epidemiological Data ◽

Hpv Infection ◽

Abnormal Cytology ◽

Hpv Genotypes ◽

Anal Cytology ◽

The Difference

Abstract Background Anorectal high-risk human papillomavirus (hr-HPV) infection is associated with anal malignancies. However, the epidemiological data is quite limited in Japan. Methods Anorectal swab was obtained from HIV-infected and –uninfected men who have sex with men (MSM). Only high-risk HPV genotypes were examined by the Hybrid Capture-based methodology. Anal cytology was determined by the modification of the Bethesda System classification. Results In total, 644 participants were included. No participant had history of HPV vaccination at inclusion. The overall prevalence of hr-HPV was 58.5% (95% confidence interval: 54.7-62.3). The most frequently detected hr-HPV genotypes in order were HPV-52, -16, and -58. Among the HPV infected subjects, 82.8% (312/377) were infected with at least one of the 9 valent vaccine covered genotypes. Incidence of abnormal cytology was positively correlated with the number of HPV genotypes infected rather than the difference in genotypes. Multivariate analysis identified the number of detected hr-HPV genotypes as well as HIV-infection were the independent risk factors for pre-cancer. Conclusion Nine valent vaccine preventable HPVs are currently prevalent among MSM in Japan. Also, increased numbers of hr-HPV genotypes were strongly associated with anorectal pre-cancer. Universal HPV vaccination should be considered for Japanese MSM.

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The Prevalence and Distribution of High Risk Human Papillomavirus Genotypes in Patients with Dysplastic Lesions: a Population Study

Acta Medica Marisiensis ◽

10.1515/amma-2015-0107 ◽

2015 ◽

Vol 61 (4) ◽

pp. 314-319

Author(s):

Voidăzan Septimiu ◽

Morariu Silviu-Horia ◽

Căpâlnă Mihai ◽

Mărginean Claudiu ◽

Dobreanu Minodora

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Public Health Problem ◽

Hpv Infection ◽

Single Infection ◽

Multiple Infections ◽

Major Public Health Problem ◽

Hpv Genotypes ◽

Hpv Types ◽

High Risk Hpv

AbstractBackground. Cervical cancer (CC) is a major public health problem worldwide. Knowledge of human papillomavirus (HPV) genotype prevalence and distribution is important for the introduction of an effective vaccination program and the corresponding epidemiological monitoring. The aim of this study was to identify and analyze the distribution of high-risk HPV genotypes.Methods. Data were collected from 136 patients for the detection of circulating HPV genotypes, where Pap test results revealed the presence of koilocytes or high risk (HR) dysplastic lesions, elements that raise the suspicion of HPV infection.Results. HPV infection was identified in 72 (55.4%) of the patients tested, 34 (47.3%) with single infection, and 38 (52.7%) with multiple infections. Twenty-two different types of HPV were identified: 14 high risk HPV types, 7 low risk HPV types, 1 probable high risk HPV type. HPV 16 was the most frequently detected (55.6%) one, it was involved in single (15 cases) and multiple (25 cases) infections, primarily associated with type 18 (12 cases), and type 52 (11 cases). The presence of HPV 18 (29.2%) and HPV 52 (23.6%) was identified after HPV type 16.Conclusions. Oncogenic HPV genotypes 16, 18, and 52 were most frequently associated in women with dysplastic lesions, which require the use of polyvalent HPV vaccines when assessing cross-protective effects of specific immunoprophylaxis programs.

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Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

BMC Infectious Diseases ◽

10.1186/s12879-021-05956-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Akouélé P. Kuassi-Kpede ◽

Essolakina Dolou ◽

Théodora M. Zohoncon ◽

Ina Marie Angèle Traore ◽

Gnatoulma Katawa ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

West Africa ◽

Public Health Problem ◽

Hpv Infection ◽

Cervical Lesions ◽

Hpv Genotypes ◽

Precancerous Cervical Lesions ◽

High Risk Hpv ◽

First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

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Human papillomavirus vaccination: evidence base for efficacy and safety

GYNECOLOGY ◽

10.26442/20795696.2021.2.200742 ◽

2021 ◽

Vol 23 (2) ◽

pp. 125-130

Author(s):

Yuliya E. Dobrokhotova ◽

Ekaterina I. Borovkova

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Hpv Vaccination ◽

Intraepithelial Neoplasia ◽

Evidence Base ◽

Hpv Infection ◽

Efficacy And Safety ◽

Human Papillomavirus Vaccination ◽

Hpv Types ◽

Further Development

The article provides a literature review on the prevention of cervical cancer by human papillomavirus (HPV) vaccination. Currently, 3 vaccines are available: the 4-valent vaccine against HPV types 6, 11, 16 and 18, the 9-valent vaccine against HPV types 6, 11, 16, 18, 31, 33, 45, 52, 58 and the bivalent vaccine against HPV types 16 and 18. Vaccination provides protection for women and men against infection with HPV and further development of HPV-associated diseases. Following immunization, seroconversion develops in 93-100% of women and in 99-100% of men and is effective in preventing incident and persistent HPV infection as well as cervical intraepithelial neoplasia. HPV immunization is ineffective in treating an existing HPV infection, genital warts, or anogenital intraepithelial neoplasia. HPV vaccination status does not affect recommendations for cervical cancer screening.

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Skin and Mucosal Human Papillomavirus Seroprevalence in Persons with Fanconi Anemia

Clinical and Vaccine Immunology ◽

10.1128/cvi.00665-14 ◽

2015 ◽

Vol 22 (4) ◽

pp. 413-420 ◽

Cited By ~ 10

Author(s):

Rachel A. Katzenellenbogen ◽

Joseph J. Carter ◽

Joshua E. Stern ◽

Melinda S. Butsch Kovacic ◽

Parinda A. Mehta ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Fanconi Anemia ◽

Hpv Vaccine ◽

Hpv Vaccination ◽

Low Risk ◽

Luminex Assay ◽

History Of ◽

Hpv Types ◽

And Behavior

ABSTRACTPersons with Fanconi anemia (FA) are at risk for human papillomavirus (HPV)-associated cancers; however, their natural HPV exposure and infection rates are unknown as is the adequacy with which they mount antibodies to HPV vaccination. This study aimed to determine, in 62 persons with FA, the seroprevalence of skin and mucosal HPV types, the seroprevalence in individuals self-reporting a history of HPV vaccination, and the factors associated with HPV seropositivity. A bead Luminex assay was used to determine seropositivity for HPV1, -2, and -4 (low-risk skin), -6 and -11 (low-risk mucosal, included in one HPV vaccine), -16 and -18 (high-risk mucosal, included in both HPV vaccines), and -52 and -58 (high-risk mucosal). Health- and behavior-related questionnaires were completed. Type-specific seroprevalence estimates and participant characteristics associated with seroprevalence were calculated; 48% reported HPV vaccination. Type-specific seropositivity in unvaccinated persons ranged from 7 to 21% for skin HPV types and 7 to 38% for mucosal HPV types. Among the unvaccinated participants, adults versus children demonstrated increased HPV1, -6, -16, and -58 seroprevalence of 45% versus 6%, 64% versus 22%, 64% versus 17%, and 36% versus 0%, respectively (allP< 0.05). The vaccinated participants versus the nonvaccinated participants demonstrated increased seroprevalence of HPV6, -11, -16, and -18 of 92% versus 38%, 92% versus 24%, 96% versus 34%, and 75% versus 7%, respectively (allP< 0.0001). Our data demonstrate that the unvaccinated participants had serologic evidence of prior skin and mucosal HPV infections and that seroprevalence increased among adults; in self-reported vaccinees, seroprevalence of HPV vaccine types was 75 to 96%.

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The Prevalence of High Carcinogenic Risk of HPV Genotypes among HIV-Positive and HIV-Negative MSM from Russia

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2021/6641888 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Ilia Beliakov ◽

Maria Senina ◽

Yuriy Tyulenev ◽

Elena Novoselova ◽

Viktor Surovtsev ◽

...

Keyword(s):

High Risk ◽

Hiv Infection ◽

Hpv Infection ◽

Carcinogenic Risk ◽

Hiv Positive ◽

Hiv Status ◽

Hpv Genotypes ◽

Hpv Types ◽

Sex With Men ◽

Hiv Negative

Objective. Men who have sex with men (MSM) have a high risk of lifelong anal cancer caused by high-risk human papillomavirus (HR HPV) infections. The aim of this study was to investigate the prevalence of anal canal HR HPV infection among men who have sex with men (MSM) with and without HIV infection in Moscow (Russia). We evaluated associations of some HIV coinfections (HSV and CMV) and HPV distribution among MSM with and without HIV infection. Methods. Two groups of HIV-positive (n = 60) and HIV-negative (n = 60) MSM were evaluated in the study. Fourteen high-risk (HR) HPV types, HSV1/2, and CMV were investigated in men anal swabs. Results. HR HPVs were found with nearly the same frequency of 66.7% in both groups: HIV-positive and HIV-negative MSM. HIV-positive status was statistically associated with the presence of several (more than two) HPV types ( p = 0.044 ). The most prevalent HR HPV genotypes were HPV18, HPV16, HPV56, and HPV33 for HIV-positive MSM and HPV56, HPV51, HPV66, and HPV16 for HIV-negatives. We found a statistically significant association of five HR HPV types with HIV status of MSM: HPV16 ( p = 0.028 ), HPV18 ( p = 0.00006 ), HPV58 ( p = 0.003 ), HPV33 ( p = 0.019 ), and HPV39 ( p = 0.026 ). The frequency of HSV1 (1.7%) and HSV2 (10%) infections and CMV (3.3%) infection was evaluated in the group of HIV-positive MSM. The frequency of HSV1 (5%) and HSV2 (6.7%) infections and CMV (0%) infection was evaluated, as well, in the group of HIV-negative MSM. Conclusion. Multiple HPV genotypes were detected significantly more often than single HPV genotype in the group of HIV-positive MSM. According to our data, 25% of HIV-positive MSM have HPV39; this is the only one of the five types of HR HPV (16, 18, 58, 33, and 39) associated with this group of MSM that has not yet been included in the HPV vaccines available on the market.

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Clinical comparison of two human papillomavirus detection assays: GenoFlow and reverse line blot

The Journal of Infection in Developing Countries ◽

10.3855/jidc.11769 ◽

2020 ◽

Vol 14 (01) ◽

pp. 97-103

Author(s):

Fatimah S Alhamlan ◽

Hadeel H Khayat ◽

Dalia A Obeid ◽

Asma M Tulba ◽

Teejan S Baduwais ◽

...

Keyword(s):

Cervical Cancer ◽

Developing Countries ◽

Human Papillomavirus ◽

Hpv Infection ◽

Reverse Line Blot ◽

Outpatient Clinics ◽

Test Results ◽

Screening Programs ◽

Hpv Genotypes ◽

Hpv Types

Introduction: Human papillomavirus (HPV) infection is typically critical in the oncogenesis of cervical cancer. However, available HPV detection kits differ in their ability and sensitivity to detect various types of HPV, and this variability has led to inconsistencies in the reporting of the geographic prevalence of HPV types, especially in developing countries. Here, we compared results of the recently developed GenoFlow HPV array test, which detects 33 HPV genotypes, to those of the well-established reverse line blot (RLB) assay, which detects 23 HPV types. Methodology: In total, 608 cervical specimens with cytology results ranging from normal to cancer were collected using an endocervical brush from women attending outpatient clinics in Riyadh, Saudi Arabia. Results: Sixty-nine specimens (11%) were positive for HPV. HPV genotype detection using the GenoFlow test had a sensitivity of 62% and a specificity of 100%. Overall agreement between the two HPV genotyping methods was 97%, with a concordance rate of 95%. Among the GenoFlow test results, 2% indicated additional HPV types that were not detected in the RLB assay, whereas the GenoFlow test missed 0.3% of the HPV types that were detected by the RLB; however, both tests were in agreement in detecting all major HPV types. Conclusion: The GenoFlow test was reliable, with results comparable to the RLB test. However, because the GenoFlow test is less labor-intensive and takes less total time (3 hours), it is a promising, affordable alternative to the RLB for HPV diagnosis and screening programs.

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Current studies on human papillomavirus in Saudi Arabia

The Journal of Infection in Developing Countries ◽

10.3855/jidc.6538 ◽

2015 ◽

Vol 9 (06) ◽

pp. 571-576 ◽

Cited By ~ 9

Author(s):

Fatimah Saeed Alhamlan ◽

Ahmed A Al-Qahtani ◽

Mohammed N Al-Ahdal

Keyword(s):

Cervical Cancer ◽

Saudi Arabia ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

World Health ◽

Cervical Lesions ◽

Hpv Types ◽

Health Organization ◽

Low Prevalence

Human papillomavirus (HPV) infection is a significant etiological factor and an important prognosticator in cervical cancer. Indeed, researchers worldwide have confirmed these roles for high-risk HVPs in over 70% of cervical cancer cases. According to the World Health Organization, approximately 561,200 new cancer cases (5.2% of all new cancers) are attributed to HPV infection. Over 120 types of HPV are classified further as either low-risk HPV (LR-HPV) or high-risk HPV (HR-HPV) based on their oncological potential of transforming cells. The LR-HPV types cause benign hyperproliferative lesions (i.e. genital warts) while the HR-HPV types are strongly associated with premalignant and malignant cervical lesions. Data on the prevalence of HPV, survival of infected patients, and mortality rate are scarce in Saudi Arabia. The unsubstantiated assumption of a low prevalence of HPV in Saudi Arabia has contributed to limiting HPV research in this conservative country. Therefore, the goal of this review is to shed light on the current HPV research being conducted and the prevalence of HPV in Saudi Arabia.

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Correlation between Human Papillomavirus Codetection Profiles and Cervical Intraepithelial Neoplasia in Japanese Women

Microorganisms ◽

10.3390/microorganisms8121863 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1863

Author(s):

Kaori Okayama ◽

Hirokazu Kimura ◽

Koji Teruya ◽

Yasuyoshi Ishii ◽

Kiyotaka Fujita ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Hpv Genotypes ◽

High Risk Type ◽

Pcr Method ◽

Polymerase Chain ◽

High Risk Hpv

Human papillomavirus (HPV) infection is thought to be strongly associated with the precarcinomatous state cervical intraepithelial neoplasia (CIN) and cervical carcinoma. To accurately assess the correlation between HPV detection profiles and CIN, the uniplex E6/E7 polymerase chain reaction (PCR) method was used. We detected HPV (37 genotypes) in 267 CIN cases. The detection of a single high-risk HPV genotype occurred in 69.7% of CIN1 and worse than CIN1 (CIN1+) cases whereas other types were detected in 11.6% of cases. Codetection of high-risk HPV genotypes occurred in 4.9% of CIN1+ cases. The high-risk genotype HPV16 was the most frequently detected genotype in CIN1+ lesions; the genotype HPV34 (not a high-risk type) was detected in some CIN3 cases. Furthermore, HPV codetection may not be associated with CIN grades. These results suggest that various HPV genotypes are associated with CIN across all analyzed cases.

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Characterization and Diversity of 243 Complete Human Papillomavirus Genomes in Cervical Swabs Using Next Generation Sequencing

Viruses ◽

10.3390/v12121437 ◽

2020 ◽

Vol 12 (12) ◽

pp. 1437

Author(s):

Ardashel Latsuzbaia ◽

Anke Wienecke-Baldacchino ◽

Jessica Tapp ◽

Marc Arbyn ◽

Irma Karabegović ◽

...

Keyword(s):

Human Papillomavirus ◽

Next Generation Sequencing ◽

High Risk ◽

Pairwise Distance ◽

Next Generation ◽

Hpv Genotypes ◽

Complete Genomes ◽

Hpv Types ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

In recent years, next generation sequencing (NGS) technology has been widely used for the discovery of novel human papillomavirus (HPV) genotypes, variant characterization and genotyping. Here, we compared the analytical performance of NGS with a commercial PCR-based assay (Anyplex II HPV28) in cervical samples of 744 women. Overall, HPV positivity was 50.2% by the Anyplex and 45.5% by the NGS. With the NGS, we detected 25 genotypes covered by Anyplex and 41 additional genotypes. Agreement between the two methods for HPV positivity was 80.8% (kappa = 0.616) and 84.8% (kappa = 0.652) for 28 HPV genotypes and 14 high-risk genotypes, respectively. We recovered and characterized 243 complete HPV genomes from 153 samples spanning 40 different genotypes. According to phylogenetic analysis and pairwise distance, we identified novel lineages and sublineages of four high-risk and 16 low-risk genotypes. In total, 17 novel lineages and 14 novel sublineages were proposed, including novel lineages of HPV45, HPV52, HPV66 and a novel sublineage of HPV59. Our study provides important genomic insights on HPV types and lineages, where few complete genomes were publicly available.

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