Microcoulometric Determination of Organochlorine Pesticides in Human Blood

1974 ◽  
Vol 57 (3) ◽  
pp. 595-603
Author(s):  
Francis D Griffith ◽  
Robert V Blanke
Keyword(s):  
Sulfuric Acid ◽  
Human Blood ◽  
Retention Time ◽  
Whole Blood ◽  
Industrial Chemicals ◽  
Chlorinated Pesticides ◽  
Human Whole Blood ◽  
Sulfuric Acid Method ◽  
Made In

Abstract Improvements in the microcoulometric halogen system allow analysis of as little as 1 ppb chlorinated pesticides with specificity and linearity. Modifications were made in the sulfuric acid method of extracting pesticides from human whole blood to obtain recovery of 24 pesticides and 7 industrial chemicals. Recovery data were tabulated. Retention time and response tables for OV-210 and SE-30/QF-1 columns were prepared for these compounds, using the microcoulometric detector.

Determination of Chlorinated Pesticides in Whole Blood

1973 ◽  
Vol 56 (5) ◽  
pp. 1173-1177
Author(s):  
Paula E Stretz ◽  
Henry M Stahr
Keyword(s):  
Sulfuric Acid ◽  
Whole Blood ◽  
Gas Liquid Chromatography ◽  
Chlorinated Pesticides ◽  
Blood Samples ◽  
Mean Values ◽  
Sulfuric Acid Method ◽  
Large Systematic Error ◽  
Good Agreement

Abstract Eight whole blood samples spiked with lindane, dieldrin, and p,p′-DDT at levels of 2.5, 12.6, 126, and 630 ppb each were sent to 11 collaborators for analysis. The method evaluated uses sulfuric acid to liberate the pesticides from the blood matrix. The pesticides are then extracted with 10% acetone in hexane. Pesticides were identified and quantitatively analyzed by gas-liquid chromatography, using an electron capture detector. Mean values at all levels are in good agreement with the spiking levels. However, statistical analysis of the data reveals a large systematic error. Further evaluation of the sulfuric acid method by comparison with other methods is recommended.

Antigen-specific T cell responses: Determination of their frequencies, homing properties, and effector functions in human whole blood

Methods ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 77-83 ◽  
Author(s):  
Tanja Breinig ◽  
Martina Sester ◽  
Urban Sester ◽  
Andreas Meyerhans
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
T Cell Responses ◽  
Human Whole Blood ◽  
Effector Functions ◽  
Cell Responses

LC–APCI–MS–MS for the Determination of Celastrol in Human Whole Blood

2007 ◽  
Vol 66 (9-10) ◽  
pp. 735-739 ◽  
Author(s):  
Qing Xu ◽  
Mei Huang ◽  
Micong Jin ◽  
Qilong Ren
Keyword(s):  
Whole Blood ◽  
Human Whole Blood

scholarly journals FORENSIC CASES IN THE NORTH OF CHILE: DETERMINATION OF ANTIDEPRESSANT DRUGS IN HUMAN WHOLE BLOOD

2013 ◽  
Vol 58 (2) ◽  
pp. 1733-1736 ◽  
Author(s):  
FELIPE BRAVO ◽  
CARMEN ZAMBRA ◽  
KARINA VENEGAS ◽  
DAVID RIOS ◽  
PEDRO BUC CALDERON ◽  
...  
Keyword(s):  
Whole Blood ◽  
Antidepressant Drugs ◽  
Human Whole Blood ◽  
The North

Simultaneous determination of methotrexate and its eight metabolites in human whole blood by capillary zone electrophoresis

2003 ◽  
Vol 1014 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Chien-Yuan Kuo ◽  
Hsin-Lung Wu ◽  
Hwang-Shang Kou ◽  
Shyh-Shin Chiou ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Capillary Zone Electrophoresis ◽  
Whole Blood ◽  
Human Whole Blood ◽  
Zone Electrophoresis ◽  
Capillary Zone

Determination of amphetamines in human whole blood by capillary electrophoresis with photodiode array detection

2002 ◽  
Vol 29 (6) ◽  
pp. 1073-1080 ◽  
Author(s):  
Gianpiero Boatto ◽  
Maria Virginia Faedda ◽  
Amedeo Pau ◽  
Battistina Asproni ◽  
Sonia Menconi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Whole Blood ◽  
Photodiode Array Detection ◽  
Human Whole Blood ◽  
Photodiode Array ◽  
Array Detection

scholarly journals OBSERVATIONS ON THE PHAGOCYTOSIS OF THE PNEUMOCOCCUS BY HUMAN WHOLE BLOOD

1930 ◽  
Vol 51 (5) ◽  
pp. 675-684 ◽  
Author(s):  
Hugh K. Ward
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Type I ◽  
Human Whole Blood ◽  
Type Iii ◽  
Number Of Individuals ◽  
Considerable Range

1. The phagocytic titre of whole human blood against the three types of pneumococcus was determined in a number of individuals. The titre varied over a considerable range in different subjects. 2. Contrary to expectation, the titre in early cases of untreated pneumonia was quite high against the infecting organism, pointing to a local rather than a general lowering of resistance in infection with this organism. 3. Sia's work was confirmed, that the specific carbohydrate has a specific anti-phagocytic action on the blood. This action is more marked in the case of Type III than in Type I.

Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: A comparison with fluorescence detection

2011 ◽  
Vol 76 (4) ◽  
pp. 277-294 ◽  
Author(s):  
Roman Kanďár ◽  
Pavla Žáková ◽  
Miroslava Marková ◽  
Halka Lotková ◽  
Otto Kučera ◽  
...  
Keyword(s):  
Detection Limit ◽  
Whole Blood ◽  
Trichloroacetic Acid ◽  
Signal To Noise Ratio ◽  
Glutathione Disulfide ◽  
Simple Method ◽  
Human Whole Blood ◽  
Coulometric Detection ◽  
Coefficients Of Variation

We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the simultaneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 × 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0 mMhydrochloric acid was stable at 4 °C for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6–97.6% for GSH and 85.0–104.4% for GSSG. The linear range is 5.0–2000.0 μmol/l, with a detection limit of 2.1 μmol/l (signal-to-noise ratio = 3) for GSH and 2.0–250.0 μmol/l, with a detection limit of 0.9 μmol/l for GSSG.

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