Volatile N-Nitrosamines in Dried Foods
Abstract An investigation was carried out to determine the levels of volatile Af-nitrosamines in several dried food commodities such as malt, instant skim milk powder, infant formula containing milk powder, instant coffee, soup and soup bases, and baby cereals. The method involves vacuum distillation of the sample from 3N KOH or 1% sulfamic acid, followed by extraction of the aqueous distillate with dichloromethane. The dichloromethane extract is then washed with an acidic buffer (to remove amines), dried over anhydrous sodium sulfate, concentrated in a Kuderna-Danish concentrator, and analyzed by gas-liquid chromatography, using a thermal energy analyzer. Detection limit is 0.1-0.5 ppb. Of the samples analyzed thus far, all malts (22 samples) and instant skim milk powders (11 samples) were positive for Af-nitrosodimethylamine (NDMA); average levels were 7.4 ppb (1.3-67.0 ppb) and 0.4 ppb (0.3-0.7 ppb), respectively. Traces of NDMA and/or JV-nitrosopyrrolidine were also detected in 3 of 20 dried soups and 5 of 10 instant coffees analyzed. Traces of NDMA and/or AT-nitrosopiperidine (NPIP) were detected in 3 of 8 powdered infant formulas. All 4 baby cereal samples were negative. The identity of NDMA in 14 samples of malt and that of NDMA and NPIP in an infant formula was confirmed by gasliquid chromatography-mass spectrometry; identity of N-nitrosamines in 3 instant coffees and 1 infant formula was independently verified by high pressure liquid chromatographic analysis.