scholarly journals Chemical Composition and Antibacterial Activity of Origanum saccatum P.H. Davis Essential Oil Obtained by Solvent-Free Microwave Extraction: Comparison with Hydrodistillation

2011 ◽  
Vol 94 (1) ◽  
pp. 243-250 ◽  
Author(s):  
Fazli Sozmen ◽  
Burcu Uysal ◽  
Birsen S Oksal ◽  
Elif Odabas Kose ◽  
I Gokhan Deniz
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Antibacterial Activities ◽  
Solvent Free ◽  
Diffusion Method ◽  
Microwave Extraction ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Oxygenated Compounds

Abstract The components of the essential oils (EOs) obtained by solvent-free microwave extraction (SFME) and hydrodistillation (HD) from endemic Origanum saccatum P.H. Davis were identified by using GC/MS. The main constituents of both EOs obtained by SFME and HD, respectively, from O. saccatum were p-cymene (72.5 and 70.6%), thymol (9.32 and 8.11%), and carvacrol (7.18 and 6.36%). The EO obtained by SFME contained substantially higher amounts of oxygenated compounds and lower amounts of monoterpenes than did the EO obtained by HD. The antibacterial activities of the EOs obtained by SFME and HD were evaluated with the disc diffusion method by comparison with 10 different bacterial strains. The antibacterial activity of the EO extracted by SFME was found to be more effective than that of the EO extracted by HD against seven of the tested bacteria.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals Investigation of antibacterial activity of cinnamyl derivatives of arylpiperazine

2012 ◽  
Vol 64 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Irena Novakovic ◽  
Jelena Penjisevic ◽  
V. Sukalovic ◽  
Deana Andric ◽  
G. Roglic ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Enteritidis ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Clostridium Sporogenes ◽  
In Vitro Antibacterial Activity ◽  
Derivatives Of

The derivatives of cinnamic acid and N-arylpiperazine show antibacterial activity. In this work the potential synergistic effect of cinnamyl derivatives of arylpiperazine in selected bacteria was investigated. The antibacterial activities of the derivatives were evaluated against Gram-positive bacteria: Staphylococcus aureus, Streptosporangium longisporum, Sarcina lutea, Micrococcus flavus, Clostridium sporogenes and Bacillus subtilis and Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis and Proteus vulgaris by the disc diffusion method. The minimum inhibitory concentration (MIC) against the selected bacteria was determined for all compounds that showed activity in the disc diffusion method. The majority of the investigated compounds displayed in vitro antibacterial activity. The effect of the type and structure of the substituent on the aromatic ring on the antibacterial activity is discussed. It was found that two derivatives expressed activity toward S. longisporum and P. aeruginosa that was almost as strong as that of amikacin.

scholarly journals The chemical composition of the essential oil and the antibacterial activities of the essential oil and methanol extract of Teucrium montanum

2008 ◽  
Vol 73 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Nenad Vukovic ◽  
Tanja Milosevic ◽  
Slobodan Sukdolak ◽  
Slavica Solujic
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Methanol Extract ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Inhibitory Effects ◽  
Disc Diffusion Method

This study was designed to examine the chemical composition of the essential oil and the in vitro antibacterial activities of the essential oil and methanol extract of Teucrium montanum. The inhibitory effects of the essential oil and the methanol extracts of Teucrium montanum were tested against thirteen bacterial species using the disc-diffusion method. GC/MS analyses revealed that the essential oil contained mainly sesquiterpenes, such as ?-cadinene (17.19 %), ?-selinene (8.16 %) and ?-calacorene (4.97 %). The highest activities were obtained with the essential oil of Teucrium montanum against K. pneumoniae, B. subtilis, B. mycoides, E. cloaceae and A. chlorococcum. In addition, comparison of the antibacterial activities of the essential oil and the methanol extract showed that the essential oil exhibited the stronger antibacterial activities.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Retrohpyllum rospigliosii Fruits from Colombia

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Clara E. Quijano-Celis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Ina Ontiveros ◽  
Leonardo Echeverri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Positive Bacterium ◽  
Disc Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
Bacterium Bacillus

The essential oil from fruits of Retrophyllum rospigliosii (Pilger) C.N. Page grown in Colombia was analyzed by GC and GC/MS. Ninety-one compounds were identified, of which the most prominent were limonene (37.7%) and α-pinene (16.3%). The in vitro antibacterial activity of the essential oil was studied against seven bacterial strains using the disc diffusion method. The strongest activity of the oil was against the Gram-positive bacterium Bacillus cereus.

scholarly journals Antibacterial Activities of Some Schiff Bases Involving Thiosemicarbazide and Ketones

2014 ◽  
Vol 26 ◽  
pp. 53-61 ◽  
Author(s):  
S.M.S. Shariar ◽  
M. Jesmin ◽  
M.M. Ali
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Schiff Bases ◽  
Cytotoxic Effect ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Three Schiff bases derived from thiosemicarbazide and ketones (Vanillin, Benzophenone and Acetophenone) were used to study their antibacterial activities against some pathogenic bacteria by disc diffusion method. Of these, benzophenone thiosemicarbazone showed significant antibacterial activity as compared with that of Kanamycin. All these three compounds were found to possess cytotoxic effect. Minimum inhibitory concentration of these compounds was also determined.

scholarly journals ANTIBACTERIAL ACTIVITY OF A COPPER(II) COMPLEX OF 1-(1H-BENZIMIDAZOL-2-YL)-N- (TETRAHYDROFURAN-2-YLMETHYL) METHANAMINE

2015 ◽  
Vol 2 (1) ◽  
pp. 11-14
Author(s):  
Elayaperumal R ◽  
Kiruthika M ◽  
Dharmalingam P
Keyword(s):  
Antimicrobial Activity ◽  
Cyclic Voltammetry ◽  
Antibacterial Activity ◽  
Free Ligand ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Infra Red ◽  
Uv Visible

A mononuclear copper(II) complex of 1-(1H-benzimidazol-2-yl)-N-(tetrahydrofuran-2- ylmethyl)methanamine) was synthesized and characterized by various physicochemical techniques like cyclic voltammetry and elemental analysis, ESI-MS, UV–Visible, Infra red and EPR spectroscopy. The antibacterial activities of the ligand and its complex were screened by disc diffusion method and found that the metal complexes have higher antimicrobial activity than the free ligand.

scholarly journals Antibacterial activities of Protein Extracts From Andrographis Paniculata, Tinospora Crispa and Centella Asiatica

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Noor Hasniza Zin ◽  
Nurul Fatihah Ahmad ◽  
Noraslinda Muhamad Bunnori ◽  
Widya Abdul Wahab ◽  
Normah Haron
Keyword(s):  
Antibacterial Activity ◽  
Centella Asiatica ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Andrographis Paniculata ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Sds Page ◽  
New Antibiotics

Introduction: Andrographis paniculata, Tinospora crispa and Centella asiatica are known to have various pharmacological functions. This research was carried out to investigate the antibacterial activities of protein extracts from A. paniculata, T. crispa and C. asiatica. Methods: Total soluble proteins from these herbs were extracted using a modified TCA/acetone method. The protein extracts were then quantified using the Bradford assay and separated using SDS-PAGE. The antibacterial activities were determined by disc diffusion method. Results: T. crispa had a significantly higher amount of proteins (83.86 ± 0.4 µg/µl) compared to A. paniculata (81.57 ± 0.4 µg/µl) and C. asiatica (78.93 ± 0.5 µg/µl). The proteins separated by SDS-PAGE were ranged from 30kDa to 260kDa, 25kDa to 110kDa and 25kDa to 160kDa for A. paniculata, T. crispa and C. Asiatic, respectively. The high abundance proteins were observed in A. paniculata and T. crispa but not in C. asitica. Protein extracts from C. asiatica have demonstrated antibacterial activity against all tested bacteria with the diameter of inhibition zone of 11.0 ± 0.5 mm, 12.3 ± 0.6 mm, 10.7 ± 0.7 mm and 20.0 ± 0.8 mm against B. cereus, S. aureus, K. pneumonia and S. typhimurium respectively. Meanwhile, protein extracts of A. paniculata showed a positive antibacterial activity only against B.cereus (13.7 ± 0.4 mm), S. aureus (7.0 ± 0.8 mm) and S. typhimurium (11.5 ± 0.3 mm). Protein extracts from T. crispa only showed a positive antibacterial activity against B. cereus (9.7 ± 0.5 mm). Conclusions: There is a constant need in the discovery of new antibiotics for the treatment of infectious diseases.

Chemical composition and antibacterial activities of Juniperus horizontalis essential oil

2014 ◽  
Vol 94 (2) ◽  
pp. 323-327 ◽  
Author(s):  
T. Eryiğit ◽  
N. Okut ◽  
K. Ekici ◽  
B. Yildirim
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
The Subject

Eryiğit, T., Okut, N., Ekici, K. and Yildirim, B. 2014. Chemical composition and antibacterial activities of Juniperus horizontalis essential oil. Can. J. Plant Sci. 94: 323–327. In recent years, the screening of antibacterial activity has been the subject of much research, and the antibacterial activity of essential oils could be a promising subject for future investigation. In this study, the antibacterial activities of Juniperus horizontalis essential oils and their components were investigated. Essential oils were isolated using the hydro-distillation method and their components were analyzed by gas chromatography–mass spectrometry (GC-MS). The main compounds found in the oil of leaves were linalool (33.76%), P-cymene (23.20%), gamma-terpinene (8.67%), trans-sabinene hydrate (8.59%), thyme camphor (8.49%), carvol (5.08%) and borneol (4.22%). Juniperus horizontalis essential oils were evaluated for antibacterial activity against six bacterial strains using the disc diffusion method. The results indicate that the essential oil of J. horizontalis shows variable and significant antibacterial activities against the six tested bacteria species. The diameters of the inhibition zones formed for bacteria were measured. The lowest inhibition zone was 12 mm (sensitive) against Pseudomonas aeruginosa ATCC 10145 and the highest zone was 32 mm (extremely sensitive) against Enterococcus faecalis ATCC 29212.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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