scholarly journals Effects of supplemental xylanase on health of the small intestine in nursery pigs fed diets with corn distillers’ dried grains with solubles

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Hongyu Chen ◽  
Shihai Zhang ◽  
Sung Woo Kim

Abstract Forty pigs [10.7 ± 1.2 kg initial body weight (BW) at 6 wk of age] were used in a 21-d study to evaluate the effects of supplemental xylanase (Hostazym X 100, Huvepharma, Inc., Peachtree City, GA) in nursery diets on digesta viscosity, nutrient digestibility, health of the small intestine, and growth performance when supplemented with corn distillers’ dried grains with solubles (DDGS). Pigs were individually housed and randomly allotted to four treatments in a 2 × 2 factorial arrangement (n = 20/factor, 0% or 30% DDGS and 0 or 1,500 endo-pentosanase unit/kg xylanase as two factors) based on sex and initial BW. Feed intake and BW were recorded weekly. On day 15 of the study, TiO2 in diets (0.3%) was used as an indigestible marker to calculate apparent ileal digestibility (AID). Plasma samples were collected on day 19 to measure tumor necrosis factor-alpha (TNF-α), malondialdehyde, and peptide YY. On day 21, all pigs were euthanized to collect tissues from duodenum, jejunum, and colon to measure morphology, TNF-α, and malondialdehyde concentrations. Distal jejunal digesta were collected to measure viscosity. Ileum digesta were collected to measure AID of nutrients. During the entire period, supplemental xylanase increased (P < 0.05) average daily gain (ADG; 616 to 660 g/d) of nursery pigs, whereas DDGS (0 or 30%) did not affect ADG. On week 3, average daily feed intake (ADFI) was increased (P < 0.05) when fed DDGS (1,141 to 1,267 g/d) and there was an interaction (P < 0.05) between two factors indicating that supplemental xylanase decreased ADFI when DDGS was used in a diet. Use of DDGS increased (P < 0.05) viscosity [1.86 to 2.38 centipoise (cP)], whereas supplemental xylanase reduced (P < 0.05) viscosity (2.27 to 1.96 cP) of jejunal digesta. The AID of dry matter (DM) and gross energy (GE) were improved (P < 0.05) by supplemental xylanase. Plasma TNF-α was decreased (P < 0.05, 108.5 to 69.9 pg/mL) by supplemental xylanase. Use of DDGS reduced (P < 0.05) villus height:crypt depth ratio (1.46 to 1.27), whereas supplemental xylanase increased (P < 0.05) the crypt depth (360 to 404 µm) in duodenum. In conclusion, feeding a diet with 30% DDGS to nursery pigs for 3 wk had no negative effect on growth performance, whereas reduced AID of DM and GE, increased TNF-α level in colon tissue, and reduced the ratio of villus height to crypt depth. Dietary supplementation of xylanase reduced digesta viscosity improving AID of nutrients, reduced inflammatory response, and altered intestinal morphology, collectively improving ADG of nursery pigs regardless of the use of DDGS in a diet.

2018 ◽  
Vol 156 (9) ◽  
pp. 1130-1137 ◽  
Author(s):  
Q. C. Ren ◽  
J. J. Xuan ◽  
X. C. Yan ◽  
Z. Z. Hu ◽  
F. Wang

AbstractThe current experiment aimed at assessing the effects of dietary supplementation of guanidino acetic acid (GAA) on growth performance, thigh meat quality and development of small intestine in broilers. A total of 360 1-day-old female broiler chicks were distributed randomly to four groups of 90 birds each, and each group received GAA dosages of 0, 0.4, 0.8 and 1.2 g/kg of feed dry matter. During the whole experiment of 60 days, broilers hadad libitumaccess to water and feed and the feed intake was recorded daily. All broilers were weighed before and after the experiment, and 30 broilers of each group were selected randomly to slaughter at the end. Increasing dietary supplementation of GAA increased final live weight and daily body weight gain, gain-to-feed ratio, thigh muscle pH value and fibre diameter of broilers, but decreased daily feed intake, drip loss, cooking loss, shear force value, hardness, gumminess and chewiness of thigh meat. In addition, increasing supplementation of GAA quadratically increased duodenal, jejunal and ileal villus height and width and ratio of villus height to crypt depth, but decreased crypt depth. The results indicated that GAA as a feed additive may support better development of small intestine, thereby resulting in improvement of growth performance and meat quality of broilers.


2020 ◽  
Vol 4 (2) ◽  
pp. 737-749
Author(s):  
Annie B Lerner ◽  
Mike D Tokach ◽  
Joel M DeRouchey ◽  
Steve S Dritz ◽  
Robert D Goodband ◽  
...  

Abstract Feeding diets high in corn distillers dried grains with solubles (DDGS) before market can negatively impact carcass yield, hot carcass weight (HCW), and belly fat iodine value (IV). Two experiments were conducted to evaluate the effects of switching from DDGS-based to corn-soybean meal (CSBM)-based diets at increasing intervals (withdrawal periods) before harvest on finishing pig performance and carcass characteristics. Diets in both experiments contained either 0% or 30% DDGS and were balanced for net energy (NE). In Exp. 1, 985 pigs (initially 99.6 kg body weight [BW]) were used with 12 pens per treatment. The four treatments were increasing DDGS withdrawal periods: 28, 21, 14, or 0 d (no dietary switch) before marketing. All pens were marketed by removing the 17% heaviest pigs 21 d before slaughter and the remaining 83% all slaughtered 21 d later. Overall, there was no evidence for treatment differences on final BW, average daily feed intake, or feed efficiency (G:F;P > 0.10); however, average daily gain (ADG) increased (linear, P = 0.022) and belly fat IV decreased (linear, P = 0.001) the longer pigs were fed CSBM diets. There was no evidence for differences for HCW (P > 0.10); however, carcass yield increased (linear, P = 0.001) with increasing time following the switch to CSBM. Backfat depth decreased and percentage lean increased as CSBM feeding time increased (quadratic; P < 0.05). In Exp. 2, 1,158 pigs (initially 105 kg BW) were used in a 35-d study. There were 15 pens per treatment and four treatments of increasing DDGS withdrawal periods: 35, 28, 14, or 0 d (no dietary switch). All pens were marketed by removing the 15% heaviest pigs on day 28, the 28% heaviest pigs on day 14, and a final marketing of approximately 57% of starting barn inventory. There was no evidence that final BW, ADG, G:F, or HCW differed among dietary treatments (P > 0.10). Average daily feed intake and carcass yield increased and belly fat IV decreased (P < 0.050); the longer pigs were fed CSBM. In conclusion, growth performance was minimally impacted following dietary switch from DDGS- to CSBM-based diets, possibly due to similar dietary NE. For carcass yield and belly fat IV, the optimal time to make a dietary switch from high to low fiber appears to be linear in nature and at least 28 d before marketing.


2004 ◽  
Vol 84 (4) ◽  
pp. 689-695 ◽  
Author(s):  
R. T. Zijlstra ◽  
S. Li ◽  
A. Owusu-Asiedu ◽  
P. H. Simmins ◽  
J. F. Patience

Fibrous components in canola meal and wheat limit their effective use in diets for weaned pigs. Effects of supplementing a carbohydrase (845 and 327 Units g-1 xylanase and β-glucanase) to a wheat-canola meal diet on growth performance, small intestine nutrient digestibility and digesta viscosity were investigated. Ninety-six weaned pigs (7.2 ± 1.2 kg) had free access to control diet [65% wheat, 25% canola meal; 3.15 Mcal digestible energy (DE) kg-1, 10.6 g digestible lysine kg-1] or control diet supplemented with carbohydrase at three inclusion rates (1, 2, or 4 g kg-1) for 28 d. Four pigs were housed per pen for six pens per diet. On days 19 to 21, six pigs per diet were euthanised to collect small intestine digesta. Carbohydrase increased average daily feed intake (ADFI) and average daily gain (ADG) quadratically (P < 0.01) for days 1 to 28, resulting in 16% higher ADFI and 13% higher ADG for 2 g kg-1 carbohydrase compared to control. Carbohydrase did not affect feed efficiency (P > 0.10). The ADG was positively correlated to ADFI (r = 0.94; P < 0.01), but not to feed efficiency (P > 0.10). Carbohydrase did not affect digestibility of dry matter (DM) and energy (P > 0.10). Carbohydrase at 4 g kg-1 only reduced digesta viscosity 30% in the distal small intestine compared to control (P < 0.05). Carbohydrase supplementation increased ADFI and thereby ADG, but not feed efficiency and nutrient digestibility, suggesting that reduction of dietary fibrous components using carbohydrase may stimulate voluntary feed intake of pigs fed diets limiting in DE. Key words: Carbohydrase, canola meal, wheat, pig, feed intake, digestibility


2018 ◽  
Vol 3 (1) ◽  
pp. 350-358
Author(s):  
Zhaohui Yang ◽  
Pedro E Urriola ◽  
Adrienne M Hilbrands ◽  
Lee J Johnston ◽  
Gerald C Shurson

Abstract The objective of this study was to use the recently determined ME and standardized ileal digestibility (SID) values of AA for a novel high-protein distillers dried grains with solubles (HP-DDGS; PureStream 40, Lincolnway Energy LLC, Nevada, IA) to determine the optimal dietary inclusion rates in diets for nursery pigs. Three hundred and sixty pigs (BW = 6.79 ± 0.02 kg) were blocked by BW, and pens within blocks were assigned randomly to one of four dietary treatments (10 pens/treatment, 9 pigs/pen). Dietary treatments consisted of adding 0%, 10%, 20%, or 30% HP-DDGS to nursery diets during phase 2 (days 7–21) and phase 3 (days 21–42) of a three-phase nursery feeding program. Diets within each phase were formulated to contain equivalent amounts of ME, SID Lys, Met, Thr, and Trp, Ca, standardized total tract digestible P, vitamins, and trace minerals. Calculated SID Leu to Lys ratios for 0%, 10%, 20%, and 30% HP-DDGS diets were 119%, 137%, 156%, and 173% in phase 2 diets and 120%, 131%, 143%, and 160% in phase 3 diets. The SID Ile to Lys ratios ranged from 60% to 69% in phase 2 diets and from 54% to 59% in phase 3 diets. The SID Val to Lys ratios ranged from 63% to 79% in phase 2 diets and 64% to 68% in phase 3 diets. Body weight and feed disappearance were measured weekly. During phase 2, ADG, ADFI, and G:F were reduced linearly (P &lt; 0.01) as the diet inclusion rate of HP-DDGS increased. Similarly in phase 3, increasing dietary levels of HP-DDGS depressed ADG, ADFI, and G:F linearly (P &lt; 0.01). Overall growth performance of phases 2 and 3 of nursery pigs was negatively affected by increasing levels of HP-DDGS in these diets. Pigs acquired a Streptococcus suis and Escherichia coli disease challenge during the experiment. Although no differences in morbidity were observed throughout the experiment, including HP-DDGS in diets tended to decrease (P = 0.08) mortality. In conclusion, a linear decrease in nursery pig growth performance was observed as increasing levels of HP-DDGS were added in diets, which was probably due to overestimation of SID AA content of the HP-DDGS, antagonistic effects of excess Leu, and the effects of relatively high fiber content.


2017 ◽  
Vol 57 (9) ◽  
pp. 1906
Author(s):  
Ghorbanali Sadeghi ◽  
Ahmad Karimi ◽  
Soosan Mohammadi ◽  
Asaad Vaziry ◽  
Mahmood Habibian

The effects of wet feeding and a multi-enzyme preparation on the nutritional value of wheat screenings (WS) for broiler chicks were investigated. In total, 540 1-day-old Ross-308 broiler chicks were fed a corn–soybean meal-based diet up to 29 days of age. At that time, the birds were randomly assigned to nine diets in a completely randomised design with a 3 × 3 factorial arrangement, including three levels of WS (0%, 25% and 50%, WS0, WS25 and WS50 respectively) and three processing methods (no processing, enzyme supplementation and wet feeding). The enzyme mixture contained endo-1, 4-β-xylanase (200 FXU/g), endo-1, 4-β-glucanase (50 FBG/g), endo-1, 8-β-glucanase (21 FBG/g), hemicellulase (3000 VHCU/g), α-galactosidase (3 GALU/g) and protease (260 U/g) and was supplemented at a level of 6 g/kg of diet. The inclusion of water in wet diets was in the extent of 1.2 kg/kg of diet. During the period from 29 to 49 days of age, feed intake was not affected (P > 0.05) by the inclusion of WS in the diet, whereas chicks fed the WS25 diet had a higher (P < 0.05) bodyweight gain than did those on WS0 diet. The feed conversion ratio (FCR) was improved (P < 0.05) by the inclusion of WS in the diet throughout the study. The feed intake was increased (P < 0.05) by enzyme supplementation to WS50 diet, while bodyweight gain was increased (P < 0.05) by wet feeding. Enzyme supplementation did not affect (P > 0.05) FCR, whereas wet feeding resulted in lower (P < 0.05) FCR over the entire study. The plasma concentration of glucose was increased (P < 0.05) by the inclusion of WS in the diet. The viscosity of the ileal digesta was higher (P < 0.05) in chicks receiving the WS50 diet than that in chicks receiving the other diets. The jejunal villus height was increased (P < 0.05) and the jejunal crypt depth was decreased (P < 0.05) by the inclusion of WS in diet. The jejunal crypt depth was greater (P < 0.05) in chicks receiving the wet diets than in chicks receiving the enzyme-supplemented diets (P > 0.05). In conclusion, the results from the present study indicated that broiler growth performance was improved by the inclusion of WS in the diet and also by wet feeding. These effects were largely compatible with the changes that occurred in the small intestine morphology, but not with those observed for ileal digesta viscosity.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 130-130
Author(s):  
Kibeom Jang ◽  
Jerry M Purvis ◽  
S W Kim

Abstract Lactose is an effective carbohydrate source for nursery pigs. Weaning age could influence needs for lactose. Weaning ages can vary among producers depending on preferred management programs. This study aimed to evaluate optimum levels of lactose for pigs at 7 kg BW weaned at different ages. A total of 1,632 pigs were weaned at d21 (d 21.2 ± 1.3) or d25 (d 24.6 ± 1.1). Pigs had a common diet until they reach 7 kg BW. Within a same weaning age group, when pigs reached 7 kg body weight, they were allotted to 4 dietary treatments with 6, 9, 12, and 15% lactose. Feed intake and BW were measured after 10d feeding when pigs reached 11 kg BW. Data were analyzed using the MIXED procedure of SAS for a slope ratio analysis to test effects of lactose levels on growth performance of pigs influenced by their weaning ages. Pigs weaned at d21 had Phase 1 for 11 days whereas pigs weaned at d 25 had Phase 1 for 2 days to reach 7 kg BW. According to the slope ratio analysis, as increasing lactose intake, ADG of pigs weaned at d21 responded greater (P &lt; 0.05, 68.5%) than growth of pigs weaned at d25. There was no change of slope in feed intake of pigs weaned at d 25 by increasing lactose intake. However, G:F of pigs weaned at d 25 responded greater (P &lt; 0.05, 250%) than G:F of pigs weaned at d21. In conclusion, pigs weaned at d21 grew faster from 7 to 11 kg BW as lactose intake increased compared with pigs weaned at d25 potentially because pigs weaned at d21 had an extended time for adaptation and recovery from weaning stress until they reached 7 kg BW.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 88-89
Author(s):  
Henrique S Cemin ◽  
Mike D Tokach ◽  
Aaron M Gaines ◽  
Brent W Ratliff ◽  
Steve S Dritz ◽  
...  

Abstract Two experiments were conducted to determine the effects of soybean meal (SBM) level in diets with or without distillers dried grains with solubles (DDGS) on growth performance of late nursery pigs. A total of 1,064 and 1,011 pigs (PIC 280×1050; initially 10.5 ± 0.36 and 10.9 ± 0.22 kg) were used in Exp. 1 and 2, respectively, with 21 to 27 pigs per pen. Pens were assigned to treatments in a randomized complete block design with 7 replicates per treatment per experiment. Treatments 1 to 3 were diets with 23% DDGS and 21, 27, or 35% SBM. Treatments 4 and 5 were corn-SBM diets with 27 or 35% SBM. Diets were balanced to 1.30% standardized ileal digestible lysine and 2,606 kcal of net energy/kg. Data were analyzed with the GLIMMIX procedure of SAS with pen as the experimental unit and block as random effect. There was no evidence for treatment × experiment interactions, thus data from both trials were combined. Feeding diets with 23% DDGS decreased (P = 0.033) ADFI and improved (P = 0.033) G:F compared to corn-SBM diets, which may indicate underestimation of DDGS net energy. When analyzed as a factorial with or without DDGS, pigs fed diets with 35% SBM had a tendency (P = 0.052) for increased ADG and improved (P = 0.001) G:F compared with diets with pigs fed 27% SBM. As SBM increased from 21 to 35% in diets with DDGS, ADG (linear, P = 0.001) and G:F (quadratic, P = 0.007) improved. In summary, feeding diets with increasing SBM improved growth performance in late nursery pigs.


Sign in / Sign up

Export Citation Format

Share Document