47 Effects of Lactobacillus and Bacillus Probiotics on Pre-ruminating Calf Growth Performance and Efficiency

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 149-150
Author(s):  
Sydney M Bowman ◽  
Steven T Quantz ◽  
Tom Rehberger ◽  
Kellen Habib ◽  
Kristen Smith ◽  
...  

Abstract Lactobacillus and Bacillus probiotics may serve as an alternative to antibiotics in milk replacer for pre-ruminant calves by altering gastrointestinal microflora to prevent the proliferation of harmful bacteria. However, there are limited studies evaluating their impact on calf growth and efficiency. The objective of this trial was to discover the impact of probiotic-containing milk replacer on pre-ruminating calf growth performance. A total of 44 pre-ruminant calves (50% Angus/50% Holstein, average weight 39.3 kg + 7.5 kg) were fed milk replacer with either no probiotic or 1.25x10^9 colony forming units/head/day of a probiotic blending containing Lactobacillus and two different Bacillus species (Church & Dwight Animal Nutrition (Princeton, NJ). Calves were blocked by age and randomly assigned within block to one of two treatments in a randomized complete block design. Calf was the experimental unit, with 22 calves per treatment. Calf weight and hip height was measured on d 0 and 30 to calculate average daily gain (ADG) and average growth per day. Calves were offered 1.36 kg milk replacer [15% dry matter (DM)] twice daily and up to 0.5 kg starter feed (88% DM) once daily. Refusals were recorded to calculate average daily intake and gain to feed ratio (G:F). Initial weight differed (P = 0.020) between treatments, and was therefore used as a covariate in all response criteria. Calves fed milk replacer with probiotic had greater (P < 0.05) ADG (0.53 vs. 0.42 kg/d, respectively) and G:F (0.50 vs. 0.41, respectively) than those fed the control. There was no evidence (P >0.05) that probiotic inclusion impacted hip height on d 30 or daily intake. In summary, Lactobacillus and Bacillus probiotic inclusion in milk replacer improved calf growth performance without impacting intake. Subsequent research is warranted to elucidate mode of action and evaluate impact relative to an antibiotic-containing positive control.

2018 ◽  
Vol 3 (1) ◽  
pp. 419-428 ◽  
Author(s):  
Cassie L Holloway ◽  
R Dean Boyd ◽  
Dean Koehler ◽  
Stacie A Gould ◽  
Qingyun Li ◽  
...  

Abstract Previous research indicates that “super-dosing” phytase may improve pig growth performance by improved nutrient use, although the benefits appear to be more consistent in nursery than in grow-out pigs. Therefore, two experiments were conducted to determine if performance could be improved by feeding phytase at super-dosed levels, and whether this response would be different if energy and amino acid (AA) were limiting. Experiment 1 involved 440 weaned pigs (6.27 ± 0.01 kg) in a factorial arrangement of treatments comparing the main effects of diet (positive control [PC] balanced for all nutrients vs. a negative control [NC]: 10% lower standardized ileal digestible (SID) lysine with relative reduction of all other essential AA and 1% reduced fat) and phytase levels (0 vs. 2,500 FTU Quantum Blue 5G phytase/kg). Pigs were assigned to pen according to a randomized complete block design based on body weight (BW). Feed and water were provided ad libitum across four dietary phases: 3 × 1 wk plus 1 × 2 wk. The average daily gain (ADG) and gain to feed ratio (G:F) were improved in the PC relative to the NC (P < 0.05) indicating success in formulating a diet limiting in energy and/or AA. Phytase improved ADG and G:F, regardless of diet composition (P < 0.05). Thus, super-dosing phytase improved nursery pig growth performance, irrespective of diet nutrient adequacy or deficit. Experiment 2 involved 2,200 growing pigs (36.6 ± 0.30 kg) allotted to five treatments: a balanced PC (250 FTU Quantum Blue 5G phytase/kg), an NC (PC with 15% less SID lysine and 1.5% lower net energy [NE]), and three super-dosing phytase treatments applied to the NC totaling 1,000, 1,750, and 2,500 FTU phytase/kg. Feed and water were available ad libitum. At trial completion (approximately 122 kg), the PC pigs were heavier and more efficient than the NC pigs (P < 0.05) indicating success in formulating an NC treatment. Super-dosing phytase had no effect on whole body ADG or average daily feed intake (P > 0.10) but tended to improve G:F and feed energy efficiency (P < 0.10). Super-dosing phytase improved carcass-based feed and feed energy efficiency (P < 0.05) and tended to improve ADG (P < 0.10). Supplying phytase at “super-dosed” levels—above that required to meet the phosphorus requirement—improved growth performance in nursery pigs (6 to 22 kg BW) and provided smaller benefits in grow-finish pigs (37 to 122 kg BW). The improvement during the nursery period was independent of energy and AA levels in the diet.


2021 ◽  
Vol 100 (1) ◽  
Author(s):  
Olufemi Oluwaseun Babatunde ◽  
Olayiwola Adeola

Abstract Two experiments were carried out to determine a time-series effect of phytase on phosphorus (P) utilization in growing and finishing pigs using growth performance, apparent total tract digestibility (ATTD) of nutrients, P excretion, and plasma concentrations of minerals as the response criteria for evaluation. In both experiments, treatments were arranged as a 3 × 4 factorial in a randomized complete block design with 3 corn–soybean meal-based diets including a P-adequate positive control (PC), a low-P negative control (NC; no inorganic P), and NC supplemented with phytase at 1,000 FYT/kg (NC + 1,000); and 4 sampling time points at days 7, 14, 21, and 28 in experiment 1, and days 14, 26, 42, and 55 in experiment 2. In both trials, 96 growing pigs with average body weight (BW) of 19.8 ± 1.16 and 49.8 ± 3.21 kg, respectively, were allocated to the 3 diets with 8 replicates pens (4 barrows and 4 gilts) and 4 pigs per pen. In experiment 1, pigs fed the PC had higher (P < 0.01) BW, average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F) when compared with pigs fed the NC. There was an interaction (P < 0.01) between time and diet on the BW and ADG of pigs while a linear and quadratic increase (P < 0.01) was observed with the ADFI and G:F, respectively, over time. Phytase supplementation improved (P < 0.01) all growth performance responses. Pigs fed the PC had greater (P < 0.01) ATTD of P and Ca than pigs fed the NC. There was no interaction effect on the ATTD of nutrients. Phytase addition improved the ATTD of P and Ca over pigs fed the NC. There was an interaction (P < 0.01) between diet and time on the total and water-soluble P (WSP) excreted. There was a quadratic decrease (P < 0.01) in plasma concentration of Ca in pigs over time. In experiment 2, there was a quadratic increase (P < 0.01) in BW, ADG, and G:F of pigs over time. Similarly, the inclusion of phytase improved (P < 0.05) all growth performance parameters except ADFI. A linear increase (P < 0.05) in the ATTD of DM, P, and Ca occurred over time. Phytase inclusion improved (P < 0.01) the ATTD of P and Ca. Plasma concentrations of P were improved by phytase addition. Phytase supplementation of the NC reduced WSP excretion by 45%, 32%, and 35% over the growing, finishing, and entire grow-finish period, respectively. In conclusion, phytase improves the utilization of P in growing and finishing pigs; however, the magnitude of effect on responses may vary over time.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Zachary K Smith ◽  
Kip Karges ◽  
Angel Aguilar

Abstract The objective of this experiment was to evaluate the influence of an active live yeast direct-fed microbial (DFM) product on receiving and backgrounding period growth performance and efficiency of dietary net energy (NE) utilization in low health risk beef steers. Maine-Anjou × Angus steers (n = 199; body weight [BW] = 252 ± 32.1 kg) were received from two sources at the Ruminant Nutrition Center in Brookings, SD, in November 2019 and used in a 77-d feedlot receiving and backgrounding experiment. Steers were provided access to long-stem hay and ad libitum water upon arrival. Steers were weighed, vaccinated for respiratory pathogens (source 2 only): infectious bovine rhinotracheitis, bovine viral diarrhea types 1 and 2, parainfluenza-3 virus, and bovine respiratory syncytial virus (Bovi-Shield Gold 5, Zoetis, Parsippany, NJ) vaccinated for clostridial species (Ultrabac 7/Somubac, Zoetis) and pour-on moxidectin (Cydectin, Bayer, Shawnee Mission, KS). Steers (n = 176 steers; initial unshrunk BW = 235 ± 27.6 kg) were allotted to pens (n = 20 pens; 10 pens per treatment; eight or nine steers per pen). Diets were based upon corn silage, dry-rolled corn, and dried distillers grains; dietary treatments were 1) no DFM (CON) and 2) DFM (Levucell SC, Advantage Titan, CNCM l-1077), fed at 10 g/steer/d providing 8 × 109 CFU of active live yeast to each steer daily (DFM). Initial BW was the average of day −1 and day 1 BW (n = 176 steers; initial BW = 253 ± 27.6 kg). On day 21, steers received a 200-mg progesterone and 20-mg estradiol benzoate implant. Data were analyzed from day 1 to 47 (receiving period), day 48 to 77, and from day 1 to 77 as a randomized complete block design; pen served as the experimental unit for all analyses. On day 47 of the experiment, DFM had greater BW (P = 0.01) by 0.9% and average daily gain (ADG; P = 0.01) by 4.2% and gain-to-feed ratio (G:F) tended (P = 0.13) to be 2.8% greater. Day 77 BW did not differ (P = 0.60), cumulative (days 1–77): ADG (P = 0.47), dry matter intake (P = 0.66), and G:F (P = 0.56) were similar. Yeast inclusion had no appreciable influence on performance-based dietary NE utilization or the ratio of observed/expected dietary NE (P ≥ 0.59). In low health risk steers, DFM improved performance during the feedlot receiving period. However, no improvements for DFM were detected for cumulative performance from day 1 to 77. The confirmation of yeast counts indicated the CFU to be above the expected level at the start of the trial but was found below expected level at the end of the trial. This may explain differences during the initial 47 d compared to cumulative growth performance results.


2020 ◽  
Vol 4 (2) ◽  
pp. 694-707
Author(s):  
Henrique S Cemin ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  

Abstract Four experiments were conducted to determine the effects of increasing soybean meal (SBM) level in diets with or without 25% distillers dried grains with solubles (DDGS) on growth performance of nursery pigs raised in university or commercial facilities. Treatments were arranged in a 2 × 3 factorial with main effects of SBM (27.5%, 32.5%, or 37.5% of the diet) and DDGS (0% or 25% of the diet). A total of 296, 2,502, 4,118, and 711 pigs with initial body weight (BW) of 10.6, 11.7, 12.5, and 12.3 kg were used in Exp. 1, 2, 3, and 4, respectively. There were 10, 16, 13, and 12 replicates per treatment in Exp. 1, 2, 3, and 4, respectively. After weaning, pigs were fed common diets for approximately 21 d. Then, pens of pigs were assigned to treatments in a randomized complete block design with BW as the blocking factor and experimental diets were fed for 21 d. Pigs were weighed and feed disappearance measured to calculate average daily gain (ADG), average daily feed intake (ADFI), gain-to-feed ratio (G:F), and caloric efficiency (CE). Data were analyzed using the GLIMMIX procedure of SAS with block as a random effect and treatment as a fixed effect. Single degree-of-freedom contrasts were constructed to test the linear and quadratic effects of increasing SBM and their interactions with DDGS. Pigs used in all experiments did not undergo major health challenges during the experimental period and due to the low number of mortality and cull events, statistical analysis was not performed on these variables. The average cull rate was 0.7%, 0.5%, 0.2%, and 0%, and the mortality rate was 0.7%, 0.3%, 0.4%, and 0% in Exp. 1–4, respectively. There were interactions (P ≤ 0.039) between SBM and DDGS for G:F and CE in Exp. 2 and for ADG and ADFI in Exp. 3. These were mostly driven by increasing SBM negatively affecting performance in a greater magnitude when diets contained DDGS compared to diets without DDGS. The main effects of DDGS and SBM were more consistently observed across experiments. Pigs fed diets with 25% DDGS had decreased (P ≤ 0.001) ADG and ADFI in all experiments, as well as poorer (P ≤ 0.028) G:F and CE except for Exp. 3. Feeding increasing amounts of SBM generally did not result in any major impact in ADG but consistently improved (linear, P ≤ 0.078) G:F and CE across experiments.


2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Spenser L Becker ◽  
Stacie A Gould ◽  
Amy L Petry ◽  
Leah M Kellesvig ◽  
John F Patience

Abstract The objective of this experiment was to evaluate the growth performance and bone mineral content (BMC) of nursery pigs in response to increasing total calcium (Ca) to available phosphorus (aP) ratios in diets containing phytase (250 FTU/kg; Natuphos E, BASF, Florham Park, NJ). A total of 480 nursery pigs (body weight (BW) = 5.7 ± 0.6 kg) with 10 pigs per pen and 7 pens per treatment (6 pens fed 2.75:1 diet) were allotted to seven treatments consisting of increasing ratios of calcium to available phosphorus (Ca:aP): 1.25, 1.50, 1.75, 2.00, 2.25, 2.50, and 2.75. From day −7 to 0, pigs were fed a common diet. They were then fed the treatment diets during two experimental phases from day 1 to 14 and 15 to 28, respectively. Available P was formulated to 0.33% and 0.27% (approximately 90% of requirement) in dietary phases 1 and 2, respectively. BW, average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F) were determined. BMC of the femur was measured on day 28 on one pig per pen using dual x-ray absorptiometry. Data were analyzed as a linear mixed model using PROC MIXED (SAS, 9.3). Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing the Ca:aP. Over the 28-d experimental period, increasing Ca:aP resulted in a linear decrease in ADG (353, 338, 328, 304, 317, 291, and 280 g/d; P < 0.01), ADFI (539, 528, 528, 500, 533, 512, and 489 g/d; P < 0.05), and G:F (0.68, 0.66, 0.64, 0.62, 0.61, 0.59, and 0.58; P < 0.01). Increasing Ca:aP also resulted in decreased BW on days 14 and 28 (P < 0.01). The BMC of the femur decreased with increasing Ca:aP (6.2, 6.3, 5.7, 5.9, 5.5, 5.6, and 5.3 g; P < 0.05). Regression analysis explained the impact of Ca:aP as follows on ADG (ADG [g/d] = 339 − 36x; r2 = 0.81), G:F (G:F = 0.61 – 0.03x; r2 = 0.72), and BMC (BMC [g] = 6.4 – 0.27x; r2 = 0.43), where x is the Ca:aP. In conclusion, all outcomes indicated that any level of calcium above the minimum used in this experiment impaired growth performance and skeletal development. Further research using even lower levels of dietary Ca is warranted.


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Hayden E Williams ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
Steven S Dritz ◽  
Michael D Tokach ◽  
...  

Abstract A total of 140 weanling pigs (241 × 600, DNA, Columbus, NE; initially 5.5 ± 0.79 kg body weight) were used in a 32-d study evaluating the effects of increasing dietary Fe from either iron sulfate (FeSO4) or iron carbonate (FeCO3) on nursery pig growth performance and blood Fe status. The pigs used for this trial did not receive an Fe injection after birth in order to increase the sensitivity to added dietary Fe after weaning. Pigs were weaned at approximately 21 d and allotted to pens based on the initial weight in a completely randomized block design with five pigs in each pen and four pens per treatment. Experimental treatments were arranged as a 2 × 3 + 1 factorial with main effects of dietary Fe source (FeSO4 vs. FeCO3) and level (10, 30, or 50 mg/kg of added Fe) plus a negative control with no additional dietary Fe. The basal diet contained 40 mg/kg total dietary Fe based on ingredient contributions and was formulated with an Fe-free trace mineral premix. Experimental diets were formulated below the pigs recommended Fe requirement based on NRC (2012) estimates. Experimental diets were fed in pellet form in a single phase for the duration of the trial. From day 0 to 32, there was no evidence for source × level interactions for growth performance, hemoglobin (Hb), or hematocrit (Hct) values. There was no evidence for a difference (P > 0.10) in dietary Fe source. Providing increasing Fe levels in the diet from either FeSO4 or FeCO3 improved (P < 0.05) average daily gain, average daily feed intake, gain-to-feed ratio, and increased (P < 0.05) Hb and Hct values. A day effect (P = 0.001) was observed for both Hb and Hct with values increasing throughout the study. Increasing dietary Fe levels in the diet from either FeSO4 or FeCO3 increased (linear; P < 0.05) Hb and Hct values on days 14, 21, and 32. In summary, these data suggest that the micronized form of FeCO3 is a source of Fe that can be added to nursery diets to yield similar responses to those observed from FeSO4 supplementation. Similar to previous research, increasing dietary Fe improved the growth performance and increased Hb and Hct values when pigs have low Fe status at weaning.


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Kimberly B Wellmann ◽  
Jessica O Baggerman ◽  
W Clay Burson ◽  
Zachary K Smith ◽  
Jongkyoo Kim ◽  
...  

Abstract A randomized complete block design experiment with 32 yearling crossbred steers (average body weight [BW] = 442 ± 17.0 kg) fed a steam-flaked corn-based diet was used to evaluate the effects of dietary Zn (KemTRACE Zn propionate 27; Kemin Industries, Inc., Des Moines, IA) supplementation on live growth performance, skeletal muscle fiber, and beta-adrenergic receptor (β-AR) characteristics during the finishing phase. Steers were blocked by BW (n = 4 blocks; 8 steers/block), assigned to pens (n = 4 steers/pen), and randomly assigned to the following treatments: control (CON; 0.0 g/[head (hd) · d] of additional Zn) or additional dietary Zn (ZnP; 1.0 g/[hd · d] additional Zn). The basal diet contained Zn (60 ppm dry matter basis) from ZnSO4; additional Zn was top-dressed at feeding. Ractopamine hydrochloride (RH; Optaflexx: Elanco Animal Health, Greenfield, IN) was included at 300 mg/(hd · d) for the final 28 d of the 111-d feeding period. Longissimus muscle biopsy samples, BW, and blood were obtained on days 0, 42, 79, and 107. Final BW was collected prior to shipping on day 111. Biopsy samples were used for immunohistochemical (IHC), mRNA, and protein analysis. Serum urea nitrogen (SUN) and nonesterified fatty acid (NEFA) concentrations were measured. Steers fed ZnP had a greater average daily gain (P = 0.02) and gain to feed ratio (G:F; P = 0.03) during the RH feeding period compared with CON. There were no differences (P > 0.05) in other growth performance variables, carcass traits, mRNA abundance, or relative protein concentration for fiber type and β-AR. Fiber types I and IIA had no differences in the cross-sectional area; however, the IIX area was greater for CON (P < 0.04) compared with ZnP and increased (P < 0.02) over time. There were no differences between treatments for the β1-AR density (P > 0.05) in skeletal muscle tissue throughout the study. A treatment × day interaction was observed in β2-AR density (P = 0.02) and β3-AR density (P = 0.02) during the RH feeding period, where the abundance of the receptors increased with ZnP but did not change in CON. Compared with CON, ZnP had greater (P < 0.01) mean NEFA concentrations. Mean SUN concentrations did increase by day (P < 0.01). Additional dietary Zn, supplied as Zn propionate, upregulates β2-AR and β3-AR and improves growth performance in feedlot steers during the RH feeding period, likely through a shift of resource utilization from lipogenesis to muscle maintenance and hypertrophy.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 57-58
Author(s):  
Uislei Orlando ◽  
Ning Lu ◽  
Carine Vier ◽  
Wayne Cast ◽  
Xun Zhou ◽  
...  

Abstract Our objective was to determine the effects of feeding different neutral detergent fiber (NDF) and standardized ileal digestible (SID) lysine (Lys) levels on performance of growing-finishing pigs from 30- to 130-kg. A total of 2,016 barrows and gilts (PIC 280×Camborough, initial BW of 29.6±0.53 kg) were allotted to a randomized complete block design with a 2×2 factorial arrangement. Dietary treatments (Table 1) consisted with two NDF levels (13% or 20%) and two SID Lys curves (PIC2008 or PIC2016). There were 21 mixed-sex pigs per pen, and 24 pens per treatment. The experiment diets were fed in 3 phases. Wheat, wheat by-products, peas, and canola oil were used with NRC (2012) loading values to achieve treatments, and all diets were formulated to be iso-caloric (2,420 kcal NE/kg). Experimental data were analyzed using generalized linear mixed models with pen as the experimental unit. Increasing NDF level resulted in a reduction (P< 0.05) in average daily gain (ADG), average daily feed intake, hot carcass weight, carcass yield, carcass ADG, and backfat depth (Table 2), and tended to decrease percentage of removals and mortality (P=0.10). Pigs fed PIC2016 Lys curve had greater ADG and carcass ADG as compared to those fed PIC2008 Lys curve (P< 0.05). There were NDF×Lys interactions (P< 0.10) for ADG, gain to feed ratio (G:F), carcass ADG, and carcass G:F. These response criteria were improved for pigs fed PIC2016 Lys curve compared to those fed PIC2008 Lys curve when NDF level was 13%; while no evidence of differences was observed at 20% NDF. Results indicate that increasing NDF level from 13 to 20% deteriorate performance of 30- to 130- kg pigs; and the improved growth performance of pigs fed PIC2016 Lys curve as compared to those fed PIC2008 Lys curve was only observed when lower NDF level was presented.


2020 ◽  
Vol 4 (2) ◽  
pp. 942-949
Author(s):  
Warren C Rusche ◽  
Julie A Walker ◽  
Zachary K Smith

Abstract One hundred ninety-two Continental × British steers [initial body weight (BW) = 420 kg (standard deviation = 24.7)] were used in a randomized complete block design finishing study to evaluate the effects of feeding two types of silage germplasm at two inclusion rates. A 2 × 2 factorial arrangement of treatments was used with either a conventional hybrid (Golden Harvest G07B39-311A, Syngenta Seeds LLC, Minnetonka, MN; CON) or a hybrid with increased expression of alpha-amylase (Syngenta Enogen Feed corn, Golden Harvest E107B3-3011A-EVT5, Syngenta Seeds, LLC; ENO) fed at either 12% (12SIL) or 24% (24SIL) of diet dry matter. Steers were blocked by source and location (source 1: first three pen replicates, n = 10 steers per pen with a fourth pen replicate of six steers per pen; source 2: one pen replicate, n = 12 steers per pen) and assigned randomly within block to treatments, resulting in five pens and 48 steers per treatment. Steers were harvested after 126 (12SIL) or 140 (24SIL) days on feed (DOF). There were no silage hybrid by inclusion rate interactions detected for live growth performance (P ≥ 0.15). Silage hybrid did not affect average daily gain (ADG), gain-to-feed ratio (G:F), or final BW (FBW; P ≥ 0.35). Feeding 24% silage reduced ADG (P = 0.04) and increased G:F (P = 0.01) but increased FBW (P = 0.02) because of greater DOF compared with 12SIL. A hybrid by inclusion rate interaction was detected (P = 0.04) for calculated yield grade (YG) with steers fed 24SIL having increased YG within CON but not ENO. Hot carcass weight and rib fat were unaffected by silage hybrid (P ≥ 0.81) but were increased by feeding 24SIL (P = 0.03 and P = 0.02, respectively). Feeding increased amounts of silage increased beef produced per hectare (P = 0.05). Source of silage did not affect feedlot growth performance of cattle but, because of slight differences in estimated silage yield, conventional silage produced more kilograms of beef per hectare (P < 0.01). Feeding increased amounts of silage reduced G:F on both a live and carcass-adjusted basis but increased kilograms of beef produced per unit of land, which is paramount to cattle feeders who grow their own feedstuffs.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 98-99
Author(s):  
Vanessa Lagos ◽  
Carrie L Walk ◽  
Hans H Stein

Abstract A 21-d experiment was conducted to test the hypothesis that the Ca requirement to maximize growth performance expressed as the standardized total tract digestible (STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca and downregulates abundance of genes related to Ca absorption (TRPV6 and S100G) and tight junction proteins (OCLN, CLDN1, and ZO1). Twenty corn-soybean meal diets were formulated using a 4 5 factorial design (0.16, 0.33, 0.42, or 0.50% STTD P and 0.14, 0.29, 0.44, 0.59, or 0.74% STTD Ca), and 640 pigs (11.1 ± 1.4 kg) were allotted to the 20 diets in a randomized complete block design. On d 21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of mRNA was determined in duodenum and ileum via quantitative RT-PCR. Data were analyzed using a response surface model by removing the terms in the model that were not significant. Interactions (P < 0.01) between dietary Ca and P for average daily gain (ADG), gain to feed ratio (G:F), and bone ash were observed (Table 1). The predicted maximum ADG (614 g), G:F (0.65), and bone ash (11.68 g) at 0.33% STTD P was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively. There was a linear negative effect (P < 0.05) of Ca on the abundance of S100G, TRPV6, OCLN, and Z01 in the duodenum and on CLDN and ZO1 in the ileum. In conclusion, the STTD Ca:STTD P ratio needed to maximize growth performance of 11- to 25-kg pigs is less than 1.40:1, if P is at the requirement. Increasing dietary Ca reduces transcellular but increases paracellular absorption of Ca.


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