330 3-nitrooxypropanol Supplementation of a Forage Diet Decreased Enteric Methane Emissions from Beef Cattle Without Affecting Apparent Total-tract Digestibility

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 186-187
Author(s):  
Aklilu Alemu ◽  
Xiu Min Zhang ◽  
Maik Kindermann ◽  
Karen A Beauchemin

Abstract Supplementation of ruminant diets with the investigational methane (CH4) inhibitor 3-nitrooxypropanol (3-NOP; DSM Nutritional Products, Switzerland) is as a promising mitigation strategy. However, most studies have examined high grain or mixed forage-concentrate diets. The objective of this study was to evaluate the effects of supplementing a forage diet with 3-NOP on rumen fermentation, CH4 emissions and apparent total-tract nutrient digestibility. Eight ruminally cannulated beef heifers (BW = 514 kg) were randomly allocated to two treatments in a crossover design with 49-d periods. Dietary treatments were: control, high-forage diet (90% forage DM basis) without 3-NOP; and NOP, control diet supplemented with 150 mg 3-NOP/kg DM. After a 14-d diet adaption, dry matter (DM) intake (DMI) was recorded daily. Rumen contents were collected on days 17 and 28 for volatile fatty acid (VFA) analysis, whereas ruminal pH was continuously monitored from day 20 to 27. Diet digestibility was measured on day 38 to 41 by total collection of feces. Enteric CH4 emissions were measured on days 46 to 49 in chambers. Dry matter intake was lower (P = 0.001) for NOP (10.0 kg/d) as compared with control (10.2 kg/d). However, DM, neutral and acid detergent fiber, and crude protein digestibilities were similar between treatments (P ≥ 0.29). Although total VFA concentration was not affected by treatment (P = 0.19), the reduction in acetate and increase in propionate proportion for NOP lowered (P < 0.001) the acetate to propionate ratio by 18% as compared with control. Mean pH was lower (P = 0.03) for control (6.46) than NOP (6.57). Furthermore, CH4 yield (g/kg DMI) was 21.6% less (P < 0.001) for NOP relative to control (25.5 g/kg DMI). Overall, the results indicate that enteric CH4 emissions were effectively decreased with 3-NOP supplementation of a forage diet without affecting apparent total-tract digestibility of nutrients.

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 267
Author(s):  
Katharina Bünemann ◽  
Maren Johannes ◽  
Rolf Schmitz ◽  
Julia Hartwiger ◽  
Dirk von Soosten ◽  
...  

The aim of the study was to examine different pH parameters, such as variations throughout the day, depending on differing concentrate feed proportions. Moreover, special attention was payed to individual variation in microbial efficiencies (microbial crude protein/fermented organic matter) and their relation to ruminal pH, nutrient flows and digestibilities. For this, cows were grouped according to microbial efficiency (more, n = 5, vs. less efficient cows, n = 4). After calving, thirteen ruminally cannulated pluriparous cows, including nine duodenally cannulated animals, were divided into groups offered rations with a lower (35% on dry matter basis, n = 7) or a higher (60% on dry matter basis, n = 6) concentrate feed proportion. Ruminal pH parameters were assessed continuously by using intraruminal probes. Nutrient flows, nutrient digestibility and microbial efficiency were determined for duodenally cannulated cows. For most ruminal pH parameters it seemed that individual variability was higher than the treatment effect. However, a positive relationship between actual concentrate intake and diurnal pH fluctuations was found. Besides, the effect of individually different microbial efficiencies was assessed. Again, there were no group differences for pH parameters. However, nutrient flows were significantly higher in more efficient cows, whereas digestibilities were lower in in more efficient cows.


2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Hamed A. A. Omer ◽  
Mohamed A. Tawila ◽  
Sawsan M. Gad ◽  
Soha S. Abdel-Magid

Abstract Background The present work aimed to investigate the impact of partial replacement yellow corn by mango seed kernels (MSK) at different levels of (0, 25, and 50%) on feed and water consumption, water metabolism, nutrient digestibility, nitrogen balance, and rumen fluid parameters. Methods Twelve mature male Rahmani sheep of about 58.39 ± 1.22 kg live body weight on average were used to investigate the influence of replacing yellow corn contents (45% in the control ration) by mango seed kernels (MSK) at different levels (0, 25, and 50%) that equal (0, 11.25, and 22.5% of total ration contents) on feed and water intakes, water metabolism, nutrient digestibility coefficients, nitrogen utilization, and some rumen fluid parameters. The animals were randomly assigned to three experimental groups (four animals in each treatment). Sheep fed rations are composed of 50% concentrate feed mixture (CFM) and 50% peanut vein hay (PVH) at portion 3% dry matter (DM) of live body weight (LBW). Results The results showed that values of crude protein (CP), crude fiber (CF), and nitrogen-free extract (NFE) of MSK and yellow corn were similar. Meanwhile, MSK has superior ether extract content compared to yellow corn; meanwhile, ash content of yellow corn was lower than ash content of MSK. All tested CFM were isonitrogenous (16.17% CP), but it differs in their contents of gross and digestible energy. On the other hand, all experimental total mixed rations (TMR) were isonitrogenous (14.52% CP) and isocaloric (4177 kcal/kg DM in average). Dietary treatments significantly (P < 0.05) decreased total dry matter intake (DMI) and crude protein intake (CPI) that expressed as (g/h/day, g/kgW0.75, and kg/100 kg LBW) or gross energy intake (GEI) and digestible energy intake (DEI) that expressed as (Mcal/h/day, kcal/kgW0.75, and Mcal/100 kg LBW). Inclusion MSK in sheep ration insignificantly (P > 0.05) increased drinking water by 20% and 6.89% for experimental groups fed TMR2 and TMR3, respectively, compared to the control one (TMR1). Incorporation MSK in the rations significantly (P < 0.05) increased insensible losses of water. Dietary treatments significantly (P < 0.05) increased nutrient digestibility coefficients of DM, organic matter (OM), CP, CF, and NFE. Meanwhile, it significantly (P < 0.05) decreased EE digestibility. On the other hand, both values of total digestible nutrient (TDN) and digestible crude protein (DCP) were significantly (P < 0.005) improved. Nitrogen retention (NR) (NR % of nitrogen intake (NI) and NR % of digested nitrogen (DN)) values were significantly (P < 0.05) increased with increasing level of MSK in the sheep ration. Inclusion MSK significantly (P < 0.05) increased ruminal pH; however, it had no significant (P > 0.05) on both ammonia nitrogen (NH3-N) and total volatile fatty acid (TFVA) concentrations. Sampling time at 3 h post feeding significantly (P < 0.05) decreased ruminal pH in comparison with before and 6 h post feeding; meanwhile, sampling time had no significant effect (P > 0.05) on NH3-N concentration. Dietary treatment significantly (P < 0.05) increased the interaction between treatments and sampling time for ruminal pH. However, no interaction between treatments and sampling time was observed for both NH3-N and TVFA concentrations. Conclusion The present results revealed that replacement up to 50% of yellow corn by MSK as alternative source of energy could be useful in feeding sheep without any adverse effect on digestion and fermentation processing. Also, it can be used to depress the rations cost.


2013 ◽  
pp. 61-73
Author(s):  
Angie Poliquit ◽  
Serena Sanchez

Twelve male growing lambs were used in a randomized complete block design to determine the effects of feeding liquid acid whey (LAW) on the performance of the animal, its dry matter intake, nutrient digestibility, weight gain and ruminal pH. Animals received a diet consisting of Napier grass (Pennisetum purpureum, Schumacher). Treatments were Napier grass plus concentrate, Napier grass plus LAW and Napier grass plus concentrate plus LAW. Individual dry matter intake (DMI), biweekly weight gain (WG) and ruminal pH were measured. Diet digestibility was determined using the total collection method. Results showed that LAW increased (p<0.05) WG and diet digestibility (dry matter, organic matter and crude protein). Liquid acid whey had an average digestibility of 54.41% for dry matter, 83.94% for organic matter and 87.56% for crude protein. Ruminal pH two hours after feeding was stable for animals with LAW alone. However, DMI was similar (p>0.05) among treatments. It can be concluded that including LAW in the diet significantly improved WG, diet digestibility and ruminal pH without affecting DMI.


2020 ◽  
Vol 45 (2) ◽  
pp. 304-315
Author(s):  
O. O. Adelusi ◽  
V. O. A. Ojo

Nutritive value of low quality crop residues is improved with addition of protein–rich byproducts (poultry litter) or chemical compounds (urea) and can serve as feed resource to ruminant during the dry season. This study investigated the nutrient digestibility and blood parameters of West African dwarf (WAD) sheep fed sugarcane top silage. Sugarcane tops were ensiled alone (control) or with 2% urea (USCT silage), 45% boiler litter (BLSCT silage) and 1% urea plus 22.5% broiler litter (UBLSCT) for 42 days. Each silage treatment contains 2% molasses. Twenty-four female West African dwarf sheep of 6-8 months of age, weighing 14.58±1.16kg were randomly assigned to the four silage treatments of six animals each in a completely randomised design for 84 days. Chemical composition of the silages was determined. Performance characteristics, apparent nutrient digestibility and blood parameters of the WAD sheep were evaluated. All data were subjected to one way Analysis of Variance. Results showed that animals fed BLSCT had significantly highest (P<0.05) total dry matter intake (64.26g/kgW ) and daily weight gain (46.03 g/day) followed by those on UBLSCT and USCT while animals fed control diet recorded the least with highest feed conversion ratio (20.62). Digestibility of all nutrients was highest (P<0.05) in sheep fed BLSCT (71.43%,71.16%, 75.66%, 64.35% and 56.82% for dry matter, organic matter, crude protein, NDF and ADF digestibility respectively) and lowest (55.71%, 57.31%, 59.98%, 39.47% and 46.92% for dry matter, organic matter, crude protein, NDF andADF digestibility respectively ) in those on control diet. Similarly, sheep fed BLSCT recorded significant highest (P < 0.05) packed cell volume (32.17%), haemoglobin (10.82g/dL) and red blood cell (13.43 x10 /L). The serum glucose, total protein, albumin, globulin and blood urea concentration were similar (P > 0.05) but higher in animals fed BLSCT (3.94 mmol/L, 71.80 g/L, 37.00 g/L, 34.80 g/L and 130.55 mg/L, respectively) USCT(3.83 mmol/L, 71.50 g/L, 35.0 g/L, 36.50 g/L and 140.65 mg/L respectively) and UBLSCT (3.75 mmol/L, 71.20 g/L, 34.50g/L, 36.70 g/L and 120.90 mg/L, respectively) compared to control. The study concluded that SCTtreated with urea or broiler litter or their combination (UBLSCT) ensiled for 42 days could be fed to ruminant during long dry season without any detrimental effect onnthe health of the animals.


2021 ◽  
Vol 39 (1) ◽  
pp. 146-152
Author(s):  
E. O Ewuola ◽  
F. A Gbore ◽  
J. T Ogunlade ◽  
G. N Egbunike

In a six-week feeding trial, a total of 48 crossbred weaned male rabbits of 7 weeks old were randomly allotted to four dietary treatments, with 12 replicates per treatment to determine growth indices and nutrient digestibility of rabbits fed fumonisin-contaminated diets. Maize grains contaminated with fumonisin B1 were incorporated into rabbit diets excluding the control diet (treatment 1, 0.13mgKg-1), at varied inclusion levels of 5.0mgKg-1, 7.5mgKg-1 and 10.0mgKg-1 fumonisin B1, constituting treatments 2, 3 and 4 respectively. The results showed that the average final live weight of rabbits fed treatments 3 and 4 were significantly (P<0.05) lower than those fed treatment 2 and the control. The average daily dry matter intake of the animals fed the dietary treatments was not significantly different. The mean daily weight gain of rabbits fed 5.0mgkg-1, 7.5mgKg-1 and 10.0mgKg-1 however declined significantly (P<0.05) by 91.23, 83.92 and 81.88% respectively, relative to the mean daily weight gain of 12.31g/rabbit/day of animals fed the control diet. The result further showed that digestibility of the nutrients of the experimental diets except crude protein, were similar among the treatments. The crude protein digestibility of rabbits fed 7.5mgKg-1and 10.0mgKg-1 were identical but significantly (P<0.05) lower than those fed 5.0mgKg-1 and the control diet. This suggests inhibition of nitrogen utilization and depressed performance in the animals fed diets containing ≥ 5.0mg fumonisin B1/Kg. Diets containing up to 7.5 mg fumonisin B1/Kg will depress live weight and daily weight gain as a result of reduced protein utilization in weaned rabbits.  


2018 ◽  
Vol 58 (12) ◽  
pp. 2329 ◽  
Author(s):  
Y. Dini ◽  
J. I. Gere ◽  
C. Cajarville ◽  
Verónica S. Ciganda

Enteric methane (CH4) emissions are directly related to the quantity and type of feed intake. Existing mitigation strategies, for example, the addition of legumes to grass-based diets and increased use of grains, have been thoroughly researched and applied in different production systems. In this paper, we propose a need to expand the capacity to mitigate enteric CH4 emissions in cattle under grazing conditions. The objective of this paper was to contribute to evaluate a mitigation strategy under grazing conditions of using contrasting levels of pasture quality. The study was performed with 20 heifers twice during the year: winter and spring. Each season, the study employed a crossover design with two treatments and two 5-day measurement periods. The treatments were two pastures with different nutritional values, including a pasture with a low quality (70% of neutral detergent fibre, 1% of ether extract, 8% of non-fibre carbohydrates), 9% of crude protein, 35% of dry matter digestibility and a pasture with a high quality (42% neutral detergent fibre, 1.3% ether extract, 24% non-fibre carbohydrates, 21% crude protein and 63% dry matter digestibility). Enteric CH4 emissions were measured with sulfur hexafluoride tracer technique. The dry matter intake (kg/day) was measured indirectly using titanium dioxide as an external marker. CH4 emissions from animals grazing the high-quality pasture were 14% lower expressed as % of gross energy intake, and 11% lower expressed by unit of dry matter intake (g CH4/kg). These results quantitative showed the alternative to mitigate CH4 emissions from grazing bovines exclusively through the improvement of the forage quality offered.


2017 ◽  
Vol 39 (3) ◽  
pp. 251
Author(s):  
Oscarina De Souza Batalha ◽  
Sonia Sena Alfaia ◽  
Frank George Guimarães Cruz ◽  
Rogério Souza de Jesus ◽  
João Paulo Ferreira Rufino ◽  
...  

The objective of this study was to evaluate the effects of acid silage meal made of pirarucu waste in diets for commercial laying hens on apparent digestibility and energy metabolism. Seventy-two Hisex White hens with 71 weeks of age were assigned to a completely randomized with two treatments (control diet and diet with 3% pirarucu waste acid silage) with six replicates of six birds each. The ensiled biomass was light brown in color, showing acidified aroma; creamy consistency; 4.38±0.11 pH; 84.16% dry matter; 40.06% crude protein; 26.82% ether extract; 9.31% mineral matter, 65.16 g kg-1 calcium and 22.90 g kg-1 phosphorus. Differences (p > 0.05) were detected in digestibility of crude protein, non-fiber carbohydrates (soluble carbohydrates), etherextract, mineral matter, metabolizable energy and metabolizable energy coefficient. Our results indicate that the acid silage mealmade of pirarucu waste can be included up to 3% in diets for laying hens, showing satisfactory nutrient digestibility and potential to be used as an energy source. 


2018 ◽  
Vol 58 (6) ◽  
pp. 1049 ◽  
Author(s):  
D. Vyas ◽  
S. M. McGinn ◽  
S. M. Duval ◽  
M. K. Kindermann ◽  
K. A. Beauchemin

The objective of the present study was to determine the dose response of the methane (CH4) inhibitor 3-nitrooxypropanol (NOP) on enteric CH4 production and dry matter intake (DMI) for beef cattle fed a high-forage or high-grain diet. Fifteen crossbred yearling steers were used in two consecutive studies (high-forage backgrounding, high-grain finishing), each designed as an incomplete block with two 28-day periods with a 7-day washout in between and treatments corresponding to six doses of NOP (0 (Control), 50, 75, 100, 150, 200 mg/kg DM). The NOP was provided in the ration daily with the dose increased gradually over the first 10 days of each period. No treatment effects were observed on overall DMI or DMI of cattle when they were in the chambers either for the high-forage (P ≥ 0.54) or high-grain (P ≥ 0.26) diet. With the high-forage diet, NOP supplementation lowered total CH4 emissions (g/day) (P = 0.05), with the response at 200 mg NOP/kg DM different from Control (P < 0.05). Similarly, CH4 emissions corrected for DMI (g/kg DMI) and as a percentage of gross energy intake were linearly reduced in the high-forage diet with supplemental NOP (P < 0.01) and responses observed at 100, 150 and 200 mg NOP/kg DM differed from Control (P < 0.05). For the high-grain diet, total CH4 emissions decreased with incremental increases in the concentration of NOP supplemented (P = 0.04) and responses observed at 150 and 200 mg/kg DM differed from Control. Similarly, linear responses were observed with CH4 emissions corrected for DMI (P = 0.04) and gross energy intake (P = 0.02), with 100–200 mg NOP/kg DM differing from Control. Overall, results from the present study demonstrated that for beef cattle fed high-forage and high-grain diets, supplementation of 100–200 mg NOP/kg DM lowered enteric CH4 emissions without inducing any negative effects on DMI.


2017 ◽  
Vol 57 (8) ◽  
pp. 1613 ◽  
Author(s):  
C. Promkot ◽  
P. Nitipot ◽  
N. Piamphon ◽  
N. Abdullah ◽  
A. Promkot

Saccharomyces cerevisiae has been used to enhance the protein level of low-protein feedstuffs such as cassava root. In the present study, fresh cassava root was grated and subjected to solid-state fermentation with S. cerevisiae to enhance the protein content. The fermentation process lasted for 21 days, followed by fermentation with yeast for 5 days. The fermented product was called yeast-fermented cassava root (YEFECAR). The YEFECAR was then evaluated as a feed ingredient at 10%, 20% and 30% dry matter in concentrate diet for dry matter intake and nutrient digestibility in non-pregnant female Brahman beef cattle (bodyweight 256 ± 11.5 kg). Four cattle were randomly given four treatments in 4 × 4 Latin square design for 21-day period, including a control diet (without fermented cassava root). Cattle were fed rice straw as roughage. The results showed that while dry matter intake was not significantly different among dietary treatments, average dairy gain tended to increase when fermented cassava root was fed at 20% in the concentrate diet. There were no significant differences in nutrient digestibility among the dietary treatments. However, neutral detergent fibre and acid detergent fibre digestibility showed an increasing trend when fermented cassava root was fed to the cattle. Likewise, fermented cassava root showed no effect on rumen microbial population, although rumen bacterial population and microbial protein tended to increase with YEFECAR addition. It was concluded that yeast-fermented cassava root at 20% in concentrate feed tended to enhance rumen bacteria population and neutral detergent fibre digestibility in cattle.


2020 ◽  
Vol 7 (1) ◽  
pp. 6-13
Author(s):  
Sabarta Sembiring ◽  
Pratiwi Trisunuwati ◽  
Osfar Sjofjan ◽  
Irfan H. Djunaidi

Sixteen crossbred growing pigs Duroc x Landrace, (10 weeks of age; initial body weight 27 ± 3.92 kg) were allotted into four treatments in a randomized block design to evaluate the effects of inclusion of fermented kepok banana corm (FKBC) in the diet on the nutrient digestibility of growing pigs. There were four treatments diets offered:  basal diets without FKBC (RO); basal diets + 7% FKCB (R1); basal diets + 14% FKCB (R2); basal diets + 21% FKCB (R3). Inclusion of 21% FKBC in the diet of pigs significantly reduced (P <0.01) dry matter intake and organic matter compared to the control diet. There were no significant different between 14% and 21% FKCB on the intake and digestibility of dry matter and organic matter of the pigs. However, inclusion of FKCB at the level of 7% showed the optimum digestibility of dry matter, organic matter, crude protein, and energy with the average value of 66.57%, 70.48%, 83,43% and 70,76%, respectively. In addition, mineral consumption and retention of Ca and P were 14.3 and 9.1 g/h, respectively with the value of mineral retention both Ca and P were 11.9 and 7.5 g/h, respectively. It can be concluded that inclusion of FKCB at the level of 7% increased dry matter digestibility and organic matter. ABSTRAK Sejumlah 16 ekor ternak babi persilangan Duroc x Landrace fase grower (berumur 10 minggu dengan bobot badan awal berkisar 27± 3,92 kg) diberi 4 macam pakan perlakuan menggunakan Rancangan Acak Kelompok (RAK) dipakai untuk mengevaluasi kecernaan nutrien. Terdapat 4 perlakuak pakan yang diberikan masing-masing adalah: pakan basal tanpa BPKF (R0); pakan basal + 7% BPKF (R1); pakan basal + 14% BPKF (R2); pakan basal + 21% BPKF (R3)   Pemberian sejumlah 21% BPKF dalam ransum sangat nyata (P<0,01) menurunkan konsumsi bahan kering dan bahan organik dibanding pakan kontrol 0 % pakan fermentasi. Penggunaan produk fermentasi pada level 14 % tidak nyata (P>0,05) berbeda terhadap konsumsi dan kecernaan bahan kering dan bahan organik dibanding pemakaian level 21%. Level 7 % penggunaan FKCB adalah angka optimum menghasilkan nilai rataan kecernaan bahan kering dan bahan organik masing-masing 66,57 dan 70,48% dan kecernaan protein kasar dan energi masing-masing 83,43 dan 70,76%. Disimpulkan bahwa penggunaan tepung BPKF pada level 7 % adalah terbaik pada variabel kecernaan bahan kering dan bahan organik.


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