scholarly journals Effects of Different Concentrate Feed Proportions on Ruminal Ph Parameters, Duodenal Nutrient Flows and Efficiency of Microbial Crude Protein Synthesis in Dairy Cows During Early Lactation

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 267
Author(s):  
Katharina Bünemann ◽  
Maren Johannes ◽  
Rolf Schmitz ◽  
Julia Hartwiger ◽  
Dirk von Soosten ◽  
...  

The aim of the study was to examine different pH parameters, such as variations throughout the day, depending on differing concentrate feed proportions. Moreover, special attention was payed to individual variation in microbial efficiencies (microbial crude protein/fermented organic matter) and their relation to ruminal pH, nutrient flows and digestibilities. For this, cows were grouped according to microbial efficiency (more, n = 5, vs. less efficient cows, n = 4). After calving, thirteen ruminally cannulated pluriparous cows, including nine duodenally cannulated animals, were divided into groups offered rations with a lower (35% on dry matter basis, n = 7) or a higher (60% on dry matter basis, n = 6) concentrate feed proportion. Ruminal pH parameters were assessed continuously by using intraruminal probes. Nutrient flows, nutrient digestibility and microbial efficiency were determined for duodenally cannulated cows. For most ruminal pH parameters it seemed that individual variability was higher than the treatment effect. However, a positive relationship between actual concentrate intake and diurnal pH fluctuations was found. Besides, the effect of individually different microbial efficiencies was assessed. Again, there were no group differences for pH parameters. However, nutrient flows were significantly higher in more efficient cows, whereas digestibilities were lower in in more efficient cows.

2009 ◽  
Vol 61 (6) ◽  
pp. 1328-1338 ◽  
Author(s):  
F.H.M. Chizzotti ◽  
O.G. Pereira ◽  
S.C. Valadares Filho ◽  
M.L. Chizzotti ◽  
M.I. Leão ◽  
...  

A trial was carried out with four Holstein x Nellore crossbred steers (225±22kg of BW) fitted with ruminal and abomasal cannulaes in a 4 x 4 Latin Square design to evaluate the intake and the total and partial apparent digestibilities of nutrients, ruminal parameters, and microbial synthesis. Diets consisted of 60% silage and 40% concentrate formulated to be isonitrogenous (12.5% of crude protein, dry matter basis). Treatments consisted of different proportions of Brachiaria brizantha grass silage and sorghum silage: 100:0; 67:33; 33:67, and 0:100%, respectively, on dry matter basis. The intake of dry matter, organic matter, crude protein, ether extract, non-fiber carbohydrates, and total digestible nutrients linearly increased (P<0.01) as levels of sorghum silage increased. The total apparent digestibility of dry matter, organic matter, and neutral detergent fiber linearly increased with sorghum silage levels (P<0.02). The highest ruminal NH3-N (13.63mg/dL) levels occurred at 2.94h post-feeding whereas the lowest ruminal pH (5.87) was measured at 5.21h post-feeding. Microbial efficiency was not affected (P>0.05) by the treatments. The use of 67% of sorghum silage and 33% of grass silage increased intake and digestibility of nutrients without affecting ruminal pH, ruminal NH3-N, and microbial efficiency.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 186-187
Author(s):  
Aklilu Alemu ◽  
Xiu Min Zhang ◽  
Maik Kindermann ◽  
Karen A Beauchemin

Abstract Supplementation of ruminant diets with the investigational methane (CH4) inhibitor 3-nitrooxypropanol (3-NOP; DSM Nutritional Products, Switzerland) is as a promising mitigation strategy. However, most studies have examined high grain or mixed forage-concentrate diets. The objective of this study was to evaluate the effects of supplementing a forage diet with 3-NOP on rumen fermentation, CH4 emissions and apparent total-tract nutrient digestibility. Eight ruminally cannulated beef heifers (BW = 514 kg) were randomly allocated to two treatments in a crossover design with 49-d periods. Dietary treatments were: control, high-forage diet (90% forage DM basis) without 3-NOP; and NOP, control diet supplemented with 150 mg 3-NOP/kg DM. After a 14-d diet adaption, dry matter (DM) intake (DMI) was recorded daily. Rumen contents were collected on days 17 and 28 for volatile fatty acid (VFA) analysis, whereas ruminal pH was continuously monitored from day 20 to 27. Diet digestibility was measured on day 38 to 41 by total collection of feces. Enteric CH4 emissions were measured on days 46 to 49 in chambers. Dry matter intake was lower (P = 0.001) for NOP (10.0 kg/d) as compared with control (10.2 kg/d). However, DM, neutral and acid detergent fiber, and crude protein digestibilities were similar between treatments (P ≥ 0.29). Although total VFA concentration was not affected by treatment (P = 0.19), the reduction in acetate and increase in propionate proportion for NOP lowered (P &lt; 0.001) the acetate to propionate ratio by 18% as compared with control. Mean pH was lower (P = 0.03) for control (6.46) than NOP (6.57). Furthermore, CH4 yield (g/kg DMI) was 21.6% less (P &lt; 0.001) for NOP relative to control (25.5 g/kg DMI). Overall, the results indicate that enteric CH4 emissions were effectively decreased with 3-NOP supplementation of a forage diet without affecting apparent total-tract digestibility of nutrients.


2012 ◽  
Vol 64 (3) ◽  
pp. 649-657
Author(s):  
F.H.M Chizzotti ◽  
O.G. Pereira ◽  
S.C. Valadares Filho ◽  
M.I Leão ◽  
K.G Ribeiro

A trial involving a 2x2 factorial design was conducted to evaluate the effect of corn silage hybrids and concentrate levels (25 and 50%) on intake and digestibility of nutrients, ruminal characteristics and microbial efficiency in steers. Four ruminal and abomasal cannulated steers (512±25kg of birth weight), were used in a 4×4 Latin square design. Treatments consisted of 75% silage A + 25% concentrate; 50% silage A + 50% concentrate; 75% silage B + 25% concentrate; and 50% silage B + 50% concentrate on dry matter (DM) basis. There were no differences in the intakes of DM, organic matter (OM), crude protein, and ether extract. The intake of non fiber carbohydrates and total digestible nutrients were positively affected by concentrate levels. The digestibility of DM and OM were also positively affected by concentrate levels. There were no effects of treatments on ruminal pH values, ruminal ammonia-N, and microbial efficiency.


2013 ◽  
pp. 61-73
Author(s):  
Angie Poliquit ◽  
Serena Sanchez

Twelve male growing lambs were used in a randomized complete block design to determine the effects of feeding liquid acid whey (LAW) on the performance of the animal, its dry matter intake, nutrient digestibility, weight gain and ruminal pH. Animals received a diet consisting of Napier grass (Pennisetum purpureum, Schumacher). Treatments were Napier grass plus concentrate, Napier grass plus LAW and Napier grass plus concentrate plus LAW. Individual dry matter intake (DMI), biweekly weight gain (WG) and ruminal pH were measured. Diet digestibility was determined using the total collection method. Results showed that LAW increased (p<0.05) WG and diet digestibility (dry matter, organic matter and crude protein). Liquid acid whey had an average digestibility of 54.41% for dry matter, 83.94% for organic matter and 87.56% for crude protein. Ruminal pH two hours after feeding was stable for animals with LAW alone. However, DMI was similar (p>0.05) among treatments. It can be concluded that including LAW in the diet significantly improved WG, diet digestibility and ruminal pH without affecting DMI.


Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 328
Author(s):  
Jonathan Pelegrin-Valls ◽  
Beatriz Serrano-Pérez ◽  
Daniel Villalba ◽  
María José Martín-Alonso ◽  
Juan Ramón Bertolín ◽  
...  

This study hypothesized that reducing the level of crude protein (CP) in lambs′ feed may improve nutrient utilization and did not negatively affect their productive efficiency, blood metabolites, oxidative status (OS) or intestinal immune barrier function. A total of 120 weaned male Ripollesa lambs (45–60 days old and 15.0 ± 1.5 kg of body weight) were used. Four feed concentrates were formulated for two different phases (growing and finishing): CP20/19 group (20% and 19% of CP on dry matter basis, for each phase, respectively) and CP18/17 group (18% and 17% of CP on dry matter basis, for each phase, respectively). Lambs were randomly assigned to feeding treatments by balancing initial body weight between groups. The reduction of dietary CP level did not impair their growth performance parameters, while it did improve the apparent digestibility of organic matter. Furthermore, the lambs of the CP18/17 group showed lower plasma urea levels with no effect on OS (malondialdehyde levels) or gastrointestinal immunity markers (gene expression of interleukin 10 (IL10), tumor necrosis factor-α (TNFA) and transforming growth factor-β1 (TGFB)).


1959 ◽  
Vol 52 (2) ◽  
pp. 230-237 ◽  
Author(s):  
D. J. C. Jones

1. Four varieties of marrow stem kale, three of thousand headed kales, Hungry Gap Kale and Rape-Kale were grown at three centres in mid-Wales. They were sampled in the early winter period and separate leaf and stem samples analysed for the proximate constituents and minerals.2. Leaf to stem ratios were measured on both a green and dry matter basis and the latter values used to calculate whole plant values for the chemical constituents.3. The leaves of the marrow stem kales were higher in dry matter than the stems, except in the case of the Purple Stem Kale. This variety resembled the thousand headed and rape-type kales in having a higher dry matter in the stems. On a dry matter basis, the leaves of all the varieties were richer than the stems in ether extract, crude protein, total ash and all the minerals except potassium and sodium. These elements occurred to a greater extent in the stems of the fleshy stemmed marrow stem kales than in the leaves. The stems were higher in crude fibre and nitrogen-free extractives.4. The leaves of the rape-type kales were higher than those of the other varieties in silica, phosphorus and potassium. They were the best source of phosphorus on a whole plant basis. The marrow stem kales had considerably higher leaf and stem values for sodium and were markedly superior to the other varieties as a source of the element.


2013 ◽  
pp. 3444-3451
Author(s):  
René Patiño P ◽  
Tanimara Soares da Silva ◽  
José C. Da Silva Filho ◽  
Mohamed Emad Nasser ◽  
Dorinha Smith Vitti ◽  
...  

ABSTRACTObjective. Evaluate the effect of increasing P intake on nutrient digestibility, and compare the true and apparent absorption coefficients of P and Ca in lambs. Materials and methods. Twenty-four Santa Ines sheep, with an average weight of 33.6 ± 1.6 kg, were distributed into four treatments (0, 2, 4 and 6 g/day of supplementary P) with forage: concentrate ratio of 70:30. The study of apparent nutrient digestibility was conducted during the first week, using the total feces collection method. During the second week, after injection of 7.4 MBq of 32P and 7.7 MBq of 45Ca, apparent (AAC) and true (TAC) absorption coefficients of P and Ca were determined. The lambs were kept in metabolic cages. Results. The increase in P intake did not affect (p>0.05) dry matter, crude protein, NDF or ADF digestibility, but the TAC of P and Ca and mineral matter digestibility decreased. The AAC was not affected (p=0.10). A cubic relationship was observed between P intake and TAC (TAC=2.16–1.95X+0.55X2-0.04X3; R2=0.38) and linear relationship with the TAC of Ca (TAC=0.559–0.03X; R2=0.26). TAC and AAC values were different (p<0.001). Conclusions. The increase in P intake doesn’t impact organic matter digestibility, but does affect P and Ca absorption. Apparent digestibility is not a reliable parameter to determine the efficiency of P and Ca absorption.


2018 ◽  
Vol 7 (2.29) ◽  
pp. 457
Author(s):  
Mohammad Firdaus Hudaya ◽  
Diah Tri Widayati ◽  
Budi Prasetyo Widyobroto ◽  
Cuk Tri Noviandi

The objective of this study was to determine the content of the feed given and reproductive status of each animal and its effect on reproduction performance in early lactation dairy cows. Seven early lactation of Friesian Holstein cows were used from local farmer. The observed variable were feedstuff from local farmer and reproductive status (post partum estrous and service per conception). The results of proximat and van soest analysis from feedstuff consist of elephant grass (Pennisetum purpureum) and concentrate feed from feed mill. The elephant grass (Pennisetum purpureum) was contained of dry matter (DM) 86.27%, Ash 18.63%, extract eter (EE) 3.84%, crude fiber (CF) 30.62%, crude protein (CP) 13.05%, neutral detergent fibre (NDF) 73.73% and acid detergent fiber (ADF) 45.31%. Concentrates feed was contained of dry matter (DM) 89.24%, Ash 15.36%, extract eter (EE) 5.37%, crude fiber (CF) 17.11%, crude protein (CP) 12.76%, neutral detergent fibre (NDF) 58.23% and acid detergent fiber (ADF) 19.20%. The results of reproductive parameters for post partum estrous (PPE) 121.43±16.672 days and service per conception (S/C) was 2.14±0.690. In conclusion the feedstuff were given less meet the requirement, and affecting the performance of reproduction.  


2019 ◽  
Vol 97 (12) ◽  
pp. 4904-4911 ◽  
Author(s):  
Charmaine D Espinosa ◽  
R Scott Fry ◽  
Matthew E Kocher ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that Cu hydroxychloride improves nutrient digestibility and alters the concentration of microbial protein in the small intestine or large intestine by pigs fed a corn-soybean meal diet or a diet based on corn, soybean meal, and distillers dried grains with solubles (DDGS). Twenty-four barrows (33.3 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a 2 × 2 factorial design with 2 levels of DDGS (0% or 45%) and 2 levels of supplemental Cu from Cu hydroxychloride (0 or 150 mg/kg). A 2-period switch back design with the 4 diets and 6 replicate pigs per diet in each period was used resulting in 12 replicate pigs per diet for the 2 periods. The initial 9 d of each period was considered an adaptation period to the experimental diets. For each period, feces were collected on days 10, 11, and 12, and ileal digesta were collected for 8 h on days 13 and 14. Results indicated that inclusion of 45% DDGS to diets reduced (P &lt; 0.05) the apparent ileal digestibility (AID) of AA and the AID and the apparent total tract digestibility (ATTD) of dry matter, gross energy, and crude protein. In contrast, inclusion of DDGS to diets increased (P &lt; 0.05) the AID and the ATTD of acid hydrolyzed ether extract and the concentration of microbial protein in the hindgut (P &lt; 0.05). However, the total concentration of volatile fatty acids (VFA) in ileal digesta and in feces from pigs fed the DDGS diets were not different from concentrations in pigs fed diets without DDGS. The AID and ATTD of dry matter, gross energy, and crude protein were not affected by dietary Cu concentrations, but the AID and ATTD of acid hydrolyzed ether extract were greater (P &lt; 0.05) in diets supplemented with Cu hydroxychloride compared with diets without Cu hydroxychloride. There was also a reduction (P &lt; 0.05) in the concentration of microbial protein and a tendency for a reduction (P &lt; 0.10) in the total concentration of VFA in feces when diets were supplemented with Cu hydroxychloride. In conclusion, supplementation of Cu hydroxychloride to diets improved AID and ATTD of acid hydrolyzed ether extract and reduced the concentration of microbial protein in the large intestine and this effect was observed in diets containing DDGS as well as in diets without DDGS.


2018 ◽  
Vol 18 (4) ◽  
pp. 610
Author(s):  
Redempta Wea ◽  
I Gusti Komang Oka Wirawan ◽  
Bernadete Barek Koten

A research aimed to evaluate nutrients digestibility in growing local male pig fed spontaneous bioconverted-tamarind seed has been done in animal feed technology laboratory of Kupang State Agricultural Polytechnic.The research used 25 local male grower pigs with body weight around 3-6 kg. The study used a randomized block design with five treatments, i.e. R0 = ration using tamarind seeds without bioconversion, R1 = ration using spontaneous bioconversion tamarinds for 24 hours, R2 = rations using spontaneous bioconversion tamarinds for 48 hours, R3 = rations using bioconversion tamarinds spontaneous for 72 hours, and R4 = rations using spontaneous bioconversion tamarinds for 96 hours. Each treatment was repeated five times in which each of them used five pigs. Parameters observed were nutrient digestibility (dry matter, crude protein, crude fat, crude fiber, and ash). The data were analyzed by using variance and continued by Duncan’s multiple range test to determine the differences between treatments. The results showed that the time of spontaneous bioconversion of tamarind seeds increased the digestibility of tamarind seed nutrients and it was concluded that the best time of spontaneous bioconversion was for 72 hours.


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