PSXV-30 Late-Breaking: Oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef cattle induced by hydrogen peroxide

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 379-380
Author(s):  
Chelsie B Dahlgren ◽  
Dishnu Sajeev ◽  
Thu Dinh ◽  
Daniel Rivera ◽  
Hunter Goodson
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Blood Plasma ◽  
Fixed Effects ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Stress Markers ◽  
Muscle Tissues ◽  
In Blood Plasma

Abstract This study examined the oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef heifers induced by hydrogen peroxide. Cattle (365 ± 38 kg; n = 18) were supplemented with ground corn and soybean hulls while grazing cool and warm-season pastures, then shipped and finished at a commercial feedlot in Iowa. Animals were blocked into three groups based on initial clusters of oxidative stress markers. Two treatments of either 20 mg hydrogen peroxide/kg BW (OX, n = 9) or 10 mL of saline (CON, n = 9) were equally and randomly assigned to animals within each group. On the day before slaughter, the OX and CON treatments were administered intravenously through the jugular vein. Blood samples were collected immediately before (T0) and 90 min after (T90) treatment and centrifuged into plasma and pre-rigor muscles were collected at the neck (splenius) during slaughter; both were frozen in liquid nitrogen and stored at -80ºC. Blood plasma and muscle tissues were analyzed for total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), and glutathione peroxidase activity (GPx). Muscle tissues were also analyzed for glutathione (GSH). The data were analyzed as a randomized complete block design with repeated measures with treatment, time, and their interaction as fixed effects. Oxidative stress treatment did not affect TAC and TBARS values (P ≥ 0.137). However, T0 plasma had 0.47 mM less (P = 0.009) and 5.84 μM more (P < 0.001) of TAC and TBARS, respectively, than T90 plasma. Although CON had 27.73 nmol/min/mL more GPx at T0 (P = 0.039), such activity did not differ in both groups at T90 (P = 0.542). Treatment did not affect oxidative stress markers in pre-rigor tissues (P ≥ 0.184). Inducing oxidative stress by hydrogen peroxide had minimal effects on biomarkers in blood and muscle tissues.

Effect of lycopene administration on oxidative stress markers in blood plasma in cysic fibrosis patients

2013 ◽  
Vol 14 ◽  
pp. S71
Author(s):  
F. Kopriva ◽  
I. Oborna ◽  
I. Fingerova ◽  
G. Wojewodka ◽  
D. Radzioch ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Plasma ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
In Blood Plasma

PSX-A-18 Late-Breaking: Oxidative stress biomarkers in blood plasma of moderately exercised horses

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 372-372
Author(s):  
Elizabeth Ott ◽  
Clay A Cavinder ◽  
Caleb O Lemley ◽  
Thu Dinh
Keyword(s):  
Oxidative Stress ◽  
Blood Plasma ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Moderate Intensity ◽  
Thiobarbituric Acid Reactive Substance ◽  
Moderate Intensity Exercise ◽  
Oxidative Stress Biomarkers ◽  
Post Exercise ◽  
In Blood Plasma

Abstract Oxidative stress by physical stressors negatively impacts the performance of equine athletes. The present study was aimed to determine oxidative biomarkers in blood plasma of exercising horses. Stock-type horses were subject to a standardized moderate intensity exercise protocol following NRC guidelines 3 times per wk for 8 wk. Blood plasma was collected in wk 1, 2, 7, and 8 immediately before and 0, 30, 60, and 90 min after exercise and analyzed for total antioxidant capacity (TAC), thiobarbituric acid reactive substance (TBARS), glutathione peroxidase activity (GPx), and superoxide dismutase activity (SOD). Data were analyzed as repeated measures with wk, d, time, and their interactions as fixed effects. The TAC on d 2 (0.40 mM trolox) were 7.5% greater than that on d 3 (P = 0.013). There were wk × d × time interactions for SOD, TBARS, and GPx (P < 0.001). The TBARS remained at d-1 wk-1 pre-exercise baseline (2.70 µM malondialdehyde) for most collection times within wk 1, 7, and 8 (P ≥ 0.058); however, TBARS increased by 0.24 to 0.41 µM on d 2 of wk 2 post-exercise (P < 0.001) and remained similarly elevated on d 3 pre- and immediately post-exercise (P < 0.001). The GPx similarly remained at baseline (172.57 µM/min; P ≥ 0.621) but increased by 48.18 to 83.36 µM/min at most collection times on d 1 and 2 of wk 2 (P ≤ 0.023). The SOD remained at baseline (167.21 µM/min; P ≥ 0.055) until increasing by 11.28 to 15.61 µM/min at 30 min post-exercise on d 1, wk 1 and at most collection times on d 3, wk 8 (P ≤ 0.043). The current study indicates the time-dependent nature of oxidative stress in relation to persistent stressors such as exercise.

PSII-8 Free amino acids in blood plasma of beef cattle under induced oxidative stress by hydrogen peroxide

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 315-316
Author(s):  
Bethaney M Penrod ◽  
Corbin R Fornes ◽  
Thu Dinh ◽  
Daniel Rivera
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Hydrogen Peroxide ◽  
Blood Plasma ◽  
Repeated Measures ◽  
Free Amino Acids ◽  
Free Amino ◽  
Fixed Effects ◽  
Block Design ◽  
In Blood Plasma

Abstract This study examined the effects of oxidative stress induced by hydrogen peroxide on free amino acids in blood plasma from beef heifers (n = 18). Cattle were supplemented with ground corn and soybean hulls to 310 to 456 kg of BW while grazing cool and warm-season pastures and were evaluated for preliminary oxidative markers; afterward, they were shipped and finished at a commercial feedlot in Iowa. Animals were blocked into three groups based on principal component analysis of oxidative stress markers and two treatments of either 20 mg hydrogen peroxide/kg BW (OX, n = 9) or 10 mL of saline (CON, n = 9) were equally and randomly assigned to animals within each group. On the day before slaughter, the OX and CON treatments were administered intravenously through the jugular vein. Blood samples were collected immediately before and 90 min after treatment, centrifuged into plasma, aliquoted, snap-frozen in liquid nitrogen, and stored at -80°C. Free amino acids in plasma were derivatized by propyl chloroformate, extracted in isooctane, and determined by gas chromatography – mass spectrometry. The data were analyzed as a randomized complete block design with repeated measures with treatment, time, and their interaction as fixed effects. Twenty-seven amino acids were quantified and sarcosine concentration was 1.5 mM greater in OX plasma than in that of CON (P = 0.064). Leucine, threonine, proline, asparagine, methionine, and α-aminobutyric acid were greater pre-treatment (P ≤ 0.006); whereas cystine was greater at 90 min post-treatment (P = 0.019). Although the effects of hydrogen peroxide injection on free amino acids in blood plasma were minimal, an increase in sarcosine concentration has been reported as a marker of induced oxidative stress.

Peripheral Oxidative Stress Markers in Patients with Bipolar Disorder during Euthymia and in Siblings

2020 ◽  
Vol 20 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Amparo Tatay-Manteiga ◽  
Vicent Balanzá-Martínez ◽  
Giovana Bristot ◽  
Rafael Tabarés-Seisdedos ◽  
Flavio Kapczinski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Valproic Acid ◽  
Uric Acid ◽  
Antioxidant Capacity ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Protein Carbonyl Content ◽  
Stress Biomarkers ◽  
Stress Markers

Aims:Oxidative stress is increased during the acute phases of bipolar disorder (BD). Our aim here was to analyze oxidative stress biomarkers in patients with BD during euthymia and their siblings.Method:A cross-sectional study was performed in euthymic patients with BD-I (n=48), unaffected siblings (n=23) and genetically unrelated healthy controls (n=21). Protein carbonyl content (PCC), total antioxidant capacity (TRAP), lipid peroxidation (TBARS) and uric acid were measured as biomarkers of oxidative stress in blood.Results:The antioxidant capacity (TRAP) was lower (p<0.001) in patients with BD compared to their siblings and controls, whereas no differences were observed in PCC, TBARS or uric acid. In patients, the concentrations of TRAP and TBARS were positively associated with the dose of valproic acid (p<0.05 and p<0.001, respectively). The concentrations of these biomarkers were not significantly associated with any of socio-demographic and clinical variables.Conclusion:A selective reduction in antioxidant capacity is present in BD during euthymia state, whereas other markers of oxidative stress are unaltered during euthymia. Siblings did not show any alterations in oxidative stress biomarkers. Oxidative stress might represent a state-dependent marker in BD. The association between treatment with valproic acid and oxidative stress markers in euthymia deserves further studies.

scholarly journals Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Aein Azarang ◽  
Omid Farshad ◽  
Mohammad Mehdi Ommati ◽  
Akram Jamshidzadeh ◽  
Reza Heidari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Steatosis ◽  
Bacillus Coagulans ◽  
Male Rats ◽  
Health Priorities ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
And Oxidative Stress

Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics ( 1 ∗ 10 9   CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics’ cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.

scholarly journals Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

2008 ◽  
Vol 19 (3) ◽  
pp. 865-876 ◽  
Author(s):  
Teresa Almeida ◽  
Marta Marques ◽  
Dominik Mojzita ◽  
Maria A. Amorim ◽  
Rui D. Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Apoptotic Cell ◽  
Redox Homeostasis ◽  
Iron Chelation ◽  
Stress Sensitivity ◽  
Premature Aging ◽  
Oxidative Stress Markers ◽  
Chronological Lifespan ◽  
Stress Markers

The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1Δ mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1Δ mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.

scholarly journals Effect of Aerobic Exercise and Hydroalcoholic Extract of Tribulus Terrestris on Mitochondrial Oxidative Stress Markers in Heart Tissue of Rats Poisoned With Hydrogen Peroxide

2021 ◽  
Vol 11 (1) ◽  
pp. 30-43
Author(s):  
Nooshin Delfani ◽  
◽  
Maghsoud Peeri ◽  
Hasan Matin Homaee ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Body Weight ◽  
Aerobic Exercise ◽  
Heart Tissue ◽  
Tribulus Terrestris ◽  
Oxidative Stress Markers ◽  
Mitochondrial Oxidative Stress ◽  
Hydroalcoholic Extract ◽  
Stress Markers

Objective: Oxidative stress can cause DNA damage and apoptosis, and leads to cardiovascular disease. This study aims to evaluate the effect of aerobic exercise combined with consumption of hydroalcoholic extract of tribulus terrestris on mitochondrial oxidative stress markers in heart tissue of rats poisoned with hydrogen peroxide. Methods: This is an experimental study conducted on 42 male Wistar rats divided randomly into seven groups of Control (poisoned without supplementation), Aerobic Exercise, Aerobic Exercise + Supplementation with 5mg/kg extract, Aerobic Exercise + Supplementation with 10 mg/kg extract, Supplementation with 5mg/kg extract, Supplementation with 10mg/kg extract, and healthy control. All groups received hydrogen peroxide (100 mg/kg body weight) for 14 days intraperitoneally. The rats in supplementation groups received hydroalcoholic extract of tribulus terrestris with doses of 5 and 10 mg/kg of body weight by gavage. Aerobic exercise was performed on a treadmill at a speed of 23 m/min for 8 weeks, 5 days per week, each for 30 min. Twenty-four hours after the last exercise session, the heart tissues of rats were collected. Data were analyzed by independent t-test, two-way ANOVA, and Bonferroni post hoc test considering a significance level of P<0.05. Results: Consumption of tribulus terrestris extracts alone and in combination with aerobic exercise led to a significant increase in the levels of O6-methylguanine-DNA-methyl transferase, prooxidants-antioxidant balance, and cytochrome C oxidase, and a significant decrease in adenosine triphosphate and malondialdehyde levels compared to the control group (P<0.05). Conclusion: It seems that regular aerobic exercise and consumption of various doses of tribulus terrestris extract is a moderating factor in mitochondrial biogenesis, and is effective in reducing DNA damage in the heart tissue of rats. Lower dosage of tribulus terrestris extract has more benefits.

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