Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity

2018 ◽  
Vol 15 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Rahman Soori ◽  
Valiollah Shahedi ◽  
Ali Akbarnejad ◽  
Siroos Choobineh
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Endurance Training ◽  
Biochemical Changes ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Purslane Seed

scholarly journals Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

2008 ◽  
Vol 19 (3) ◽  
pp. 865-876 ◽  
Author(s):  
Teresa Almeida ◽  
Marta Marques ◽  
Dominik Mojzita ◽  
Maria A. Amorim ◽  
Rui D. Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Apoptotic Cell ◽  
Redox Homeostasis ◽  
Iron Chelation ◽  
Stress Sensitivity ◽  
Premature Aging ◽  
Oxidative Stress Markers ◽  
Chronological Lifespan ◽  
Stress Markers

The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1Δ mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1Δ mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.

scholarly journals Effect of Aerobic Exercise and Hydroalcoholic Extract of Tribulus Terrestris on Mitochondrial Oxidative Stress Markers in Heart Tissue of Rats Poisoned With Hydrogen Peroxide

2021 ◽  
Vol 11 (1) ◽  
pp. 30-43
Author(s):  
Nooshin Delfani ◽  
◽  
Maghsoud Peeri ◽  
Hasan Matin Homaee ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Body Weight ◽  
Aerobic Exercise ◽  
Heart Tissue ◽  
Tribulus Terrestris ◽  
Oxidative Stress Markers ◽  
Mitochondrial Oxidative Stress ◽  
Hydroalcoholic Extract ◽  
Stress Markers

Objective: Oxidative stress can cause DNA damage and apoptosis, and leads to cardiovascular disease. This study aims to evaluate the effect of aerobic exercise combined with consumption of hydroalcoholic extract of tribulus terrestris on mitochondrial oxidative stress markers in heart tissue of rats poisoned with hydrogen peroxide. Methods: This is an experimental study conducted on 42 male Wistar rats divided randomly into seven groups of Control (poisoned without supplementation), Aerobic Exercise, Aerobic Exercise + Supplementation with 5mg/kg extract, Aerobic Exercise + Supplementation with 10 mg/kg extract, Supplementation with 5mg/kg extract, Supplementation with 10mg/kg extract, and healthy control. All groups received hydrogen peroxide (100 mg/kg body weight) for 14 days intraperitoneally. The rats in supplementation groups received hydroalcoholic extract of tribulus terrestris with doses of 5 and 10 mg/kg of body weight by gavage. Aerobic exercise was performed on a treadmill at a speed of 23 m/min for 8 weeks, 5 days per week, each for 30 min. Twenty-four hours after the last exercise session, the heart tissues of rats were collected. Data were analyzed by independent t-test, two-way ANOVA, and Bonferroni post hoc test considering a significance level of P<0.05. Results: Consumption of tribulus terrestris extracts alone and in combination with aerobic exercise led to a significant increase in the levels of O6-methylguanine-DNA-methyl transferase, prooxidants-antioxidant balance, and cytochrome C oxidase, and a significant decrease in adenosine triphosphate and malondialdehyde levels compared to the control group (P<0.05). Conclusion: It seems that regular aerobic exercise and consumption of various doses of tribulus terrestris extract is a moderating factor in mitochondrial biogenesis, and is effective in reducing DNA damage in the heart tissue of rats. Lower dosage of tribulus terrestris extract has more benefits.

scholarly journals Effects of Exercise Training under Hyperbaric Oxygen on Oxidative Stress Markers and Endurance Performance in Young Soccer Players: A Pilot Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Carlos Burgos ◽  
Carlos Henríquez-Olguín ◽  
David Cristóbal Andrade ◽  
Rodrigo Ramírez-Campillo ◽  
Oscar F. Araneda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Endurance Training ◽  
Hyperbaric Oxygen ◽  
Endurance Performance ◽  
Soccer Players ◽  
Peak Power Output ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Before And After

The aim of the present study was to determine the effects of three weeks of hyperbaric oxygen (HBO2) training on oxidative stress markers and endurance performance in young soccer players. Participants (18.6±1.6 years) were randomized into hyperbaric-hyperoxic (HH) training (n=6) and normobaric normoxic (NN) training (n=6) groups. Immediately before and after the 5th, 10th, and 15th training sessions, plasma oxidative stress markers (lipid hydroperoxides and uric acid), plasma antioxidant capacity (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid [TROLOX]), arterial blood gases, acid-base balance, bases excess (BE), and blood lactate analyses were performed. Before and after intervention, maximal oxygen uptake (VO2max) and peak power output (PPO) were determined. Neither HH nor NN experienced significant changes on oxidative stress markers or antioxidant capacity during intervention. VO2max and PPO were improved (moderate effect size) after HH training. The results suggest that HBO2 endurance training does not increase oxidative stress markers and improves endurance performance in young soccer players. Our findings warrant future investigation to corroborate that HBO2 endurance training could be a potential training approach for highly competitive young soccer players.

PSXV-30 Late-Breaking: Oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef cattle induced by hydrogen peroxide

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 379-380
Author(s):  
Chelsie B Dahlgren ◽  
Dishnu Sajeev ◽  
Thu Dinh ◽  
Daniel Rivera ◽  
Hunter Goodson
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Blood Plasma ◽  
Fixed Effects ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Stress Markers ◽  
Muscle Tissues ◽  
In Blood Plasma

Abstract This study examined the oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef heifers induced by hydrogen peroxide. Cattle (365 ± 38 kg; n = 18) were supplemented with ground corn and soybean hulls while grazing cool and warm-season pastures, then shipped and finished at a commercial feedlot in Iowa. Animals were blocked into three groups based on initial clusters of oxidative stress markers. Two treatments of either 20 mg hydrogen peroxide/kg BW (OX, n = 9) or 10 mL of saline (CON, n = 9) were equally and randomly assigned to animals within each group. On the day before slaughter, the OX and CON treatments were administered intravenously through the jugular vein. Blood samples were collected immediately before (T0) and 90 min after (T90) treatment and centrifuged into plasma and pre-rigor muscles were collected at the neck (splenius) during slaughter; both were frozen in liquid nitrogen and stored at -80ºC. Blood plasma and muscle tissues were analyzed for total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), and glutathione peroxidase activity (GPx). Muscle tissues were also analyzed for glutathione (GSH). The data were analyzed as a randomized complete block design with repeated measures with treatment, time, and their interaction as fixed effects. Oxidative stress treatment did not affect TAC and TBARS values (P ≥ 0.137). However, T0 plasma had 0.47 mM less (P = 0.009) and 5.84 μM more (P &lt; 0.001) of TAC and TBARS, respectively, than T90 plasma. Although CON had 27.73 nmol/min/mL more GPx at T0 (P = 0.039), such activity did not differ in both groups at T90 (P = 0.542). Treatment did not affect oxidative stress markers in pre-rigor tissues (P ≥ 0.184). Inducing oxidative stress by hydrogen peroxide had minimal effects on biomarkers in blood and muscle tissues.

Oxidative stress markers in women with polycystic ovary syndrome without insulin resistance

2018 ◽  
Author(s):  
Reveka Gyftaki ◽  
Sofia Gougoura ◽  
Nikolaos Kalogeris ◽  
Vasiliki Loi ◽  
George Koukoulis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Oxidative Stress Markers ◽  
Ovary Syndrome ◽  
Stress Markers

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

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