scholarly journals The effect of injectable vitamin C and road transit duration on inflammation, muscle fatigue, and performance in pre-conditioned beef steer calves

Author(s):  
Aubree M Beenken ◽  
Erin L Deters ◽  
Stephanie L Hansen

Abstract This study examined the effects of injectable vitamin C (VC) before transport and duration of transit on feedlot performance, inflammation, and muscle fatigue in cattle. One hundred thirty-two Angus-cross steers (393 ± 4 kg) were stratified by body weight (BW) to a 2 × 2 factorial of intramuscular injection (INJ; 20 mL/steer): VC (250 mg sodium ascorbate/mL) or saline (SAL) and road transit duration (DUR): 18 h (18-h; 1,770 km) or 8 h (8-h; 727 km). On d 0, steers were weighed and given INJ of VC or SAL immediately before transport. Upon return (d 1), BW and blood were collected before steers returned to pens equipped with GrowSafe bunks. Steers were weighed on d 0, 1, 7, 15, 30, 31, 54, and 55. Data were analyzed via ProcMixed of SAS (experimental unit = steer; 32 to 34 steers/treatment) with fixed effects of INJ, DUR, and the interaction. Blood was collected on d -5, 1, 2, 3, and 7 (n = 9 steers/treatment); blood parameters were analyzed as repeated measures with the repeated effect of day. Area under the curve (AUC) for plasma ferric reducing antioxidant power (FRAP) was calculated using R. Final BW was greater for 8-h compared to 18-h (P = 0.05) with no effect of INJ or interaction (P ≥ 0.51). Dry matter intake (DMI) from d 1 to 7 was greater for VC-8, intermediate for VC-18 and SAL-18, and least for SAL-8 (P = 0.02). Overall DMI tended to be greatest for SAL-18, intermediate for VC-18 and VC-8, and lowest by least for SAL-8 (P = 0.08). Day 7 to 31 gain:feed (G:F) was greatest for VC-18 compared to other treatments (INJ × DUR, P = 0.05), and there was no effect of treatment on overall G:F (P ≥ 0. 19). There was no INJ or INJ × DAY (P ≥ 0.17) effect on serum lactate, haptoglobin, or non-esterified fatty acid. However, these blood parameters were greater on d 1 for 18-h compared to 8-h, and both treatments returned to near baseline by d 3 (DUR × DAY, P < 0.01). Plasma ascorbate concentrations on d 1 were greater for VC compared to SAL and returned to baseline by d 2 (INJ × DAY, P < 0.01). Plasma FRAP AUC from d -5 to 3 was greatest for VC-18, intermediate for VC-8 and SAL-8, and lowest by least for SAL-18 (INJ × DAY, P = 0.02). This suggests an antioxidant prior to long-haul transit positively influenced antioxidant capacity; however, VC did not improve overall post-transit performance. While longer transit duration increased indicators of muscle fatigue and inflammation, post-transit performance was not appreciably different between transit durations.

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 121-121
Author(s):  
Aubree M Beenken ◽  
Erin L Deters ◽  
Stephanie L Hansen

Abstract This study examined the effects of injectable vitamin C (VC) before transport and duration of transit on feedlot performance, inflammation, and muscle fatigue in cattle. One hundred thirty-one, Angus-cross steers (409 ± 4 kg) were stratified by bodyweight (BW) to a 2 × 2 factorial of intramuscular injection (INJ; 20 mL/steer): VC (250 mg sodium ascorbate/mL) or saline (SAL) and road transit duration (DUR): 18 (18; 1,770 km) or 8 h (8; 727 km). On d 0, steers were weighed and received INJ of SAL or VC immediately before transport. Upon return (d 1), BW and blood were collected before steers returned to pens with GrowSafe bunks. Steers were weighed on d 0, 1, 7, 15, 30, 31, 54, and 55. Data were analyzed via ProcMixed of SAS (experimental unit = steer; 32–34 steers/treatment) with fixed effects of INJ, DUR, and the interaction. Blood was collected on d -5, 1, 2, and 3 (9 steers/treatment); blood parameters were analyzed as repeated measures. Average daily gain (ADG) and BW were greater on d 7 and 15 for SAL-18 compared to all other treatments (INJ × DUR, P < 0.01). Final BW, overall ADG, and gain:feed were greater for 18 than 8 (P < 0.01). Injection did not affect BW (P > 0.13) but VC decreased overall dry matter intake compared to SAL (P = 0.03). Steers transported for 18 h had greater serum lactate, haptoglobin, and non-esterified fatty acid concentrations on d 1 compared to steers transported for 8 h (DUR × DAY, P < 0.01). Day 1 plasma ascorbate concentrations were greater for VC and returned to baseline concentrations by d 2 (INJ × DAY, P < 0.01). In contrast to previous work, VC did not improve post-transit performance; however, longer transit duration increased indicators of muscle fatigue and inflammation.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 34-34
Author(s):  
Erin Deters ◽  
Stephanie L Hansen

Abstract This study sought to determine the effects of injectable vitamin C (VC), pre- or post-transit, on blood parameters and feedlot performance of beef steers. Seventy-two, Angus-cross steers were blocked by weight (356 ± 18 kg) and randomly assigned to intramuscular injection treatments (20 mL/steer): saline pre- and post-transit (CON), VC (Vet One; 250 mg sodium ascorbate/mL) pre-transit and saline post-transit (PRE), or saline pre-transit and VC post-transit (POST). Following pre-transit injections (d 0), steers were transported for ~18 h (1,675 km). Upon return (d 1), steers received post-transit injections and were sorted into pens (6 steers/pen) equipped with GrowSafe bunks. Steers were weighed on d 0, 1, 7, 30, 31, 56, and 57. Blood was collected on d 0, 1, 2, and 7. Data were analyzed as a randomized complete block design using ProcMixed of SAS (experimental unit = steer; 24 steers/treatment); treatment and block were fixed effects. Blood variables were analyzed as repeated measures. Injectable VC did not affect BW shrink due to transit (P = 0.28). Compared to CON-steers, PRE or POST-steers exhibited greater dry matter intake from d 31-57 and overall (d 1-57; P ≤ 0.02). Average daily gain was greatest for PRE-steers from d 7-31 and overall (P ≤ 0.05), resulting in PRE-steers being heaviest on d 30/31 (P = 0.03) and tending to be heaviest on d 56/57 (P = 0.07). Plasma ascorbate concentrations were decreased immediately post-transit for CON and POST-steers but increased for PRE-steers (treatment × day; P < 0.01). Plasma ferric reducing antioxidant potential and malondialdehyde were decreased post-transit while serum non-esterified fatty acid and haptoglobin were increased; all blood parameters returned to baseline by d 7 (day; P < 0.01). Timing of injectable VC administration appears to influence how cattle respond to transit as pre-transit administration improved subsequent performance of steers.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 152-153
Author(s):  
Aubree M Beenken ◽  
Erin L Deters ◽  
Colten W Dornbach ◽  
Stephanie L Hansen ◽  
Joshua C McCann ◽  
...  

Abstract Ninety-one early-weaned (65 ± 11 d) Angus steers (92 ± 4 kg) were blocked by age to a 2 × 2 factorial examining effects of injectable vitamin C (VC) at weaning and/or prior to transport to the feedlot on antibody titers and growth performance. Injections (20 mL/steer) of VC (250 mg sodium ascorbate/mL) or saline (SAL) were given at time of weaning on d 0 (WEAN) and/or prior to a 6 hr trucking event to a feedlot on d 49 (TRANS). Steers were given booster vaccinations on d 0. Steers were weighed on d 0, 1, 14, 48, 49, 64, 106, and 107. Blood was collected (12 steers/treatment) on d 0, 1, 2, 14, 49 (pre- and post-transit), 50, and 51. Data were analyzed via Proc-Mixed of SAS (experimental unit = steer; n = 22–23/treatment) with fixed effects of block, WEAN, TRANS, and WEAN × TRANS. Plasma ascorbate concentrations for weaning (d 0, 1, and 2) and transit (d 49-pre-trucking, 49-post-trucking, 50, and 51) were analyzed as repeated measures (repeated effect = day). Plasma ascorbate concentrations were greater on d 1 and 2 for steers that received VC at weaning (VC = 19.6, SAL = 8.8 ± 1.26 µM; WEAN × day P < 0.01). Similarly, ascorbate concentrations were greater on d 49 post-trucking, 50, and 51 for steers that received VC pre-transit (TRANS × day P = 0.01). Treatments did not affect bodyweight or average daily gain throughout the trial (P > 0.32). There were no effects of treatment on serum Bovine Viral Diarrhea Virus type 2 antibody titers on d 14 or 51 (P > 0.33). An injection of VC administered to early weaned beef steers at weaning or pre-transit increases plasma ascorbate concentrations but does not improve growth performance or antibody response to vaccination booster.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Dalton Humphrey ◽  
Spenser Becker ◽  
Jason Lee ◽  
Keith Haydon ◽  
Laura Greiner

Abstract Four hundred and eighty (PIC 337 X 1050, PIC Genus, Hendersonville, TN) pigs were used to evaluate a novel threonine source (ThrPro, CJ America Bio, Fort Dodge, IA) for nursery pigs from approximately 7 to 20 kg body weight (BW). After weaning, pigs were sorted by sex and fed a common diet for 1 wk. Upon completion of the first week, pigs were sorted into randomized complete blocks, equalized by weight, within 16 replications. Pigs were allocated to one of three dietary treatments: positive control (POS)—standard ileal digestible threonine-to-lysine ratio (SID; Thr:Lys) 0.60, negative control (NEG)—SID Thr:Lys ≤0.46, and alternative Thr source (TEST)—SID Thr:Lys 0.60. The alternative Thr source included fermentative biomass and was assumed to contain 75% Thr and a digestibility coefficient of 100% based on the manufacturer’s specifications. All other nutrients met or exceeded the NRC recommendations. Growth and intake data were analyzed as repeated measures with a compound symmetry covariance structure using the MIXED procedure in SAS 9.4 (SAS Institute Inc., Cary, NC) with pen as the experimental unit. Treatment, phase, the interaction between treatment and phase, and block were included as fixed effects in the model. Differences in total removals were tested using Fisher’s Exact Test of PROC FREQ. Results were considered significant at P ≤ 0.05 and considered a trend at P > 0.05 and P ≤ 0.10. During the first 14 d, pigs fed TEST had decreased gain-to-feed ratio (G:F; 0.77 vs. 0.80, P = 0.022) compared to POS and increased G:F (0.77 vs. 0.73, P < 0.001) compared to NEG. Over days 14–28, pigs fed TEST had similar G:F (0.71 vs. 0.70, P = 0.112) compared to POS and increased G:F (0.71 vs. 0.63, P < 0.001) compared to NEG. Overall (days 0–28), pigs fed TEST had similar average daily gain (ADG; 0.47 vs. 0.47 kg/d, P = 0.982) and G:F (0.76 vs. 0.74, P = 0.395) compared to POS and increased ADG (0.47 vs. 0.43 kg/d, P < 0.001) and G:F (0.76 vs. 0.67, P < 0.001) compared to NEG. The average daily feed intake was not significantly different across treatments for the entirety of the study. In conclusion, the replacement of crystalline L-Thr with a novel Thr source resulted in similar growth performance in nursery pigs from approximately 7 to 20 kg.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 93-93
Author(s):  
Jesus A Acosta ◽  
Gwendolyn M Jones ◽  
John F Patience

Abstract The objective of this study was to evaluate the impact of deoxynivalenol (DON), derived from contaminated corn, on growth performance and blood metabolites. A total of 32 growing pigs (initial BW 73.1 ± 1.2 kg; L337 × Camborough, PIC, Hendersonville, TN) were randomly allotted in individual pens to 1 of 2 treatments: a non-contaminated control diet (CTL) and a diet containing 1.8 mg DON/kg (CTL+DON). Upon analysis, CTL+DON also contained 1.9 mg zearalenone/kg and 1.0 mg total fumonisins/kg. On d 1, blood samples were obtained at 60, 120 and 240 min following resumed access to feed. Pigs had ad libitum access to feed thereafter and for 28 d in total. Data were analyzed using the MIXED procedure of SAS with initial BW, sex and treatment as fixed effects, and blood parameters the repeated measures. Compared with CTL, pigs fed CTL+DON had decreased final BW (102.9 vs 87.5 kg; P < 0.001), ADG (1.06 vs 0.51 kg; P < 0.001) and ADFI (2.97 vs 1.54 kg; P < 0.001). Mycotoxins did not affect G:F (0.35 vs 0.32; P = 0.234 for CTL and CTL+DON, respectively). Compared to CTL, pigs fed CTL+DON had increased levels of blood cholecystokinin, a hormone involved in satiety and digestion (32.1 vs 43.3 pg/mL; P = 0.048), increased ghrelin, a hormone that stimulates appetite and promotes fat deposition (345 vs 654 pg/mL; P = 0.011), a tendency for decreased blood urea nitrogen (19.6 vs 17.1 mg/dL; P = 0.076), and increased creatine kinase (2,826 vs 4,920 IU/L; P = 0.027). No treatment differences were observed for circulating creatinine, glucose, albumin, or alkaline phosphatase. In conclusion, mycotoxin contamination substantially impaired feed intake and growth. Pigs offered mycotoxin-contaminated feed had altered blood parameters related to appetite regulation and nutrient metabolism. However, it was not possible to determine if these differences were due to the mycotoxin contamination or to reduced feed intake.


Author(s):  
Elizabeth M Messersmith ◽  
Stephanie L Hansen

Abstract Seventy-two Angus-crossbred steers (411 ± 16 kg) were assigned to a 2 × 3 factorial arrangement of treatments to examine the effects of blended Zn source supplementation on performance, carcass characteristics, and trace mineral parameters of steers administered no implant or a two-implant program. Factors included implant (IMP) strategies and Zn supplementation. During the 126-d study steers were either not implanted (NoIMP) or implanted (IS/200; Elanco, Greenfield, IN) on d 0 (Component TE-IS; 80 mg trenbolone acetate + 16 mg estradiol) and 57 (Component TE-200; 200 mg trenbolone acetate + 20 mg estradiol). All steers were fed 70 mg Zn/kg on a dry matter (DM) basis from ZnSO4 + 30 mg Zn/kg DM from either basic ZnCl (Vistore Zn, Phibro Animal Health, Teaneck, NJ), Zn glycinate (Gemstone Zn, Phibro Animal Health), or ZnSO4 (ZnB, ZnG, or ZnS, respectively). Steers were blocked by weight into pens of 6 and fed a dry rolled corn-based diet via GrowSafe bunks (GrowSafe Systems Ltd.; Airdrie, AB, Canada). Data were analyzed using the Mixed Procedure of SAS, with fixed effects of Zn, IMP, and the interaction. Steer was the experimental unit (n = 12 steers/treatment). Liver and muscle collected on d -5, 14, 71, and 120 were analyzed for Zn concentration, and data were analyzed as repeated measures (repeated effect = Day). An IMP × Zn tendency (P = 0.07) was observed for d 126 body weight with no effects of Zn within NoIMP while ZnS tended to be heavier than ZnB with ZnG intermediate within IS/200. Carcass-adjusted overall feed efficiency (G:F) was greatest for ZnS (Zn; P = 0.02). Implanted cattle had greater DM intake, G:F, and carcass-adjusted performance (P ≤ 0.01). Liver Zn concentrations were greater for IS/200 by d 120 (IMP × Day; P = 0.02). Within IS/200, ZnG tended to have greater muscle Zn than ZnS while ZnB was intermediate (Zn × IMP; P = 0.09). No Zn or IMP × Zn (P ≥ 0.12) effects were observed for carcass data. However, IS/200 had greater hot carcass weight, dressing percentage, and ribeye area than NoIMP (P ≤ 0.001). These data suggest implants improve growth and influence Zn metabolism. Future work should examine Zn sources and supplementation alongside implant strategies.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 63-64
Author(s):  
Spenser Becker ◽  
Laura L Greiner

Abstract The objective of this study was to investigate the effects of dietary metabolizable energy level and the ratio of linoleic acid:linolenic acid (n6:n3) on the growth performance and inflammatory status of grow-finish pigs. A total of 240 growing pigs (BW = 41.5 ± 6.1 kg) were randomly assigned to either a high (3.55 Mcal/kg; HE) or low (3.29 Mcal/kg; LE) energy dietary treatment with a high (23:1) or low (12:1) n6:n3 in a 2 x 2 factorial design (n = 16). Diets were fed across three 28-day phases and were balanced for linoleic acid inclusion. Pigs were housed 4 pigs per pen. Blood samples were collected on weeks 1, 3, 6, and 12 of the study. Synovial fluid was collected from the hock joint on weeks 1 and 12 for inflammatory marker analysis. The pen was the experimental unit and data were analyzed as repeated measures using PROC MIXED (SAS 9.4) with energy, ratio, and the interaction as fixed effects. Compared to LE, pigs receiving HE had increased BW (P &lt; 0.05) at d28 (73.0 vs. 69.9kg), d56 (105.0 vs. 100.7kg), and d84 (135.3 vs. 129.9kg). For the overall period, HE had increased ADG compared to LE (1.10 vs. 1.06kg; P &lt; 0.05) and improved G:F (0.41 vs. 0.37; P &lt; 0.05), while LE increased ADFI compared to HE (2.88 vs. 2.72kg; P &lt; 0.05). There was no effect of ratio or energy ratio interaction for growth performance. C-reactive protein tended to be reduced in hock synovial fluid of pigs receiving LE (1854.3 vs. 2277.3ng/mL; P &lt; 0.10). Across all treatments, CRP was reduced in the synovial fluid and plasma in week 12 compared to week 1 (P &lt; 0.05). In conclusion, dietary n6:n3 ratio did not impact growth performance or CRP response regardless of energy level.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 157-158 ◽  
Author(s):  
Katherine D Vande Pol ◽  
Naomi Cooper ◽  
Andres Tolosa ◽  
Michael Ellis ◽  
Caleb M Shull ◽  
...  

Abstract Newborn piglets experience a rapid decrease in body temperature, increasing the risk of mortality. The objective of this study was to determine the effect of drying and/or warming at birth on piglet rectal temperature over 24 h after birth. The study was carried out at a commercial sow facility using a CRD with 4 treatments: Control (no drying or warming of piglets), Drying (piglets dried at birth using a cellulose-based desiccant), Warming (piglets placed in a box under a heat lamp for 30 min after birth), and Drying+Warming (piglets dried and warmed as above). Piglets were identified with a numbered ear tag. They were weighed at birth and rectal temperature was measured at 0, 10, 20, 30, 45, 60, 120, and 1440 min (24 h) after birth. Sows and litters were randomly allotted at birth to treatments. Data were analyzed using a repeated measures model with PROC MIXED of SAS. Litter was the experimental unit and piglet was a subsample of the litter; the model included the fixed effects of treatment and repeated time, and the interaction. Rectal temperature at birth was similar (P > 0.05) for all treatments. Subsequently, piglets on the Drying and Warming treatments had similar (P > 0.05) temperatures, which were greater (P 0.05) than the Control at all measurement times up to 120 min. Drying+Warming resulted in the highest (P < 0.05) rectal temperature overall and at most times of measurement between 10 and 120 min. Rectal temperatures were similar for all treatment at 24 h after birth. In conclusion, drying and/or warming piglets at birth significantly increased rectal temperatures between 10 and 120 minutes after birth with the combination of the two having the greatest effect. This research was funded by the National Pork Board.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 168-168
Author(s):  
Erin L Deters ◽  
Aubree M Beenken ◽  
Stephanie L Hansen

Abstract Transportation of cattle by road is unavoidable in beef production due to segmentation of the industry. Psychological and physical stress associated with transit can negatively impact cattle health and performance upon arrival at their destination. Thus, investigation of resiliency or recovery-based nutritional strategies to mitigate transit stress are warranted. Because oxidative stress is likely linked to transportation stress, one such strategy is antioxidants such as vitamin C (VC). Seventy-two Angus-cross steers (356 ± 18 kg) were used to determine the effects of a pre- versus post-transit VC injection on 56 d feedlot performance. Steers were randomly assigned to intramuscular injection treatments (24 steers/treatment): saline pre- and post-transit (CON), VC (Vet One; 250 mg sodium ascorbate/mL; 5 g sodium ascorbate/steer) pre-transit and saline post-transit (PRE), or saline pre-transit and VC post-transit (POST). Steers were transported for ~18 h (1,675 km) prior to sorting into pens equipped with GrowSafe bunks (6 steers/pen). Blood was collected from 12 steers/treatment on d 0, 1, 2, and 7 for analysis of plasma ascorbate concentrations. Plasma ascorbate concentrations were decreased by ~10% immediately post-transit for CON and POST-steers but increased for PRE-steers; regardless of treatment, concentrations were similar to pre-transit values by d 7 (treatment × day; P &lt; 0.01). Steers that received VC at either timepoint (pre- or post-transit) exhibited greater dry matter intake from d 31–57 and d 1–57 compared to CON-steers (P ≤ 0.02). However, PRE-steers exhibited the greatest average daily gain from d 7–31 and d 1–57 (P ≤ 0.05), resulting in PRE-steers being heaviest on d 30/31 (P = 0.03) and tending to be heaviest on d 56/57 (P = 0.07). Based on these data, timing of nutritional intervention is vital for optimal effectiveness. Furthermore, nutritional strategies that improve antioxidant status prior to long-distance transit events may positively influence post-transit performance.


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