scholarly journals PSXII-8 Effects of fat matrix and particle size of coated methionine on by-pass fraction of dry matter and nitrogen

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 413-414
Author(s):  
Leandro Royo ◽  
Mónica Puyalto ◽  
Trinidad De Evan ◽  
Juan José Mallo ◽  
Maria Dolores Carro

Abstract The aim of this study was to evaluate the By-Pass fraction (BPF) of the dry matter (DM) and nitrogen (N) of coated methionine with different fat matrix (FM) and particle size (PS). Two FM (Hydrogenated Stearin, HS, and Hydrogenated Palm Fatty Acid Distillate, HPFAD) and four PS (< 500, 500–750, 750–1400, and >1400 µm) were evaluated in an in situ trial with three rumen-cannulated sheep. Intact samples (1 g) were weighed into nylon bags and incubated in the rumen for 2, 4, 8, 12 and 24 h; process repeated twice in each sheep. After extraction from the rumen, the bags were washed with cold water, frozen and lyophilized, before determining N content in the incubation residue. Effective degradability of DM and N was calculated for a rumen passage rate of 0.08/h-1 according to Ørskov and McDonald (1979), and the BPF was calculated as 100 minus the effective degradability. Data were analyzed using a mixed-effect model, with FM, PS and FMxPS interaction as fixed effects and sheep as random; means were compared with LSD test (P ≤ 0.05). HS-coated methionine had higher values of BPF of DM and N (78.2 and 49.6%, respectively; values averaged for all PS; P < 0.001) than HPFAD-coated methionine (64.4 and 17.7%, respectively). There was an interaction between FM and PS (P < 0.001) for BPF of DM and N. Increasing PS of HS-methionine augmented the BPF from 68.9 to 90.3% for DM and from 22.9 to 79.8% for N, whereas the increases for PFAD-methionine were lower (from 66.3 to 66.3% for DM, and from 7.5 to 28.6% for N). It is concluded that the use of HS as FM and the increase of PS are valid strategies to improve methionine protection from ruminal degradation, reaching BPF values of 90.3 and 79.8 % for DM and N, respectively.

1992 ◽  
Vol 72 (1) ◽  
pp. 71-81 ◽  
Author(s):  
B. M. Mosimanyana ◽  
D. N. Mowat

The effects of processing variables on soybean crude protein (CP) ruminal degradation were investigated. Soybean meal (SBM) was heated in a forced-air oven (90 °C, 1 h) with blood (0, 5, 10 and 20% dry matter) and/or xylose (3 mol mol−1 SBM-blood lysine) in a randomized complete block design. In another experiment, whole soybeans were utilized using the following treatments: raw; roasted (in Gem Co. unit exit temperature 150 °C) and steeped for 0 or 2 h; roasted, flaked (exit temperature 111 °C) and steeped for 0, 1, 2, 3 h or 1 h with 4% xylose and/or 10% blood. Solubility of SBM CP was reduced (P < 0.01) by the addition of xylose, without adverse effects on pepsin-digestible CP and acid detergent insoluble nitrogen. In situ degradation of CP (EDCP), assuming a passage rate of 5% h−1, of SBM was reduced by the addition of blood (P < 0.05) and particularly xylose (P < 0.01). Soybean CP solubility was reduced (P < 0.01) by roasting and flaking (65.6 vs. 17.6% total CP). Not flaking the roasted beans further reduced (P < 0.01) CP solubility (to 10.4%) probably due to less rapid cooling. The EDCP of raw soybeans (87.6%) was reduced by roasting (64.2%), steeping whole (57.6%) or flaked (61.1%) beans. These data support xylose to effectively reduce ruminal degradation of SBM and simple steeping (1 h) with or without flaking to further reduce EDCP of roasted soybeans. Key words: Soybean meal, soybeans, xylose, blood, steeping, protein degradation


1992 ◽  
Vol 72 (4) ◽  
pp. 881-889 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. Bittman ◽  
L. J. Fisher

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P < 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P < 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers


2018 ◽  
Vol 2 (4) ◽  
pp. 428-438 ◽  
Author(s):  
Renan L Miorin ◽  
Lucia Holtshausen ◽  
Vern Baron ◽  
Karen A Beauchemin

Abstract The objective of this in situ study was to evaluate the rumen degradability of kernels from short-season corn hybrids grown for silage in Western Canada (Lacombe, AB) and determine whether decreasing kernel particle size would enhance ruminal degradability in a similar manner for all hybrids. The study was a completely randomized design with 3 beef cows (replicates) and a 6 (hybrid) × 3 (particle size) factorial arrangement of treatments. Kernels were processed to generate three different particle sizes: large (2.3 mm), medium (1.4 mm), and small (0.7 mm). Processed samples were incubated in the rumen for 0, 3, 6, 12, 24, and 48 h using the in situ method and degradation kinetics of DM and starch were determined. Effective rumen degradability (ED) was estimated using a passage rate of 0.04 (ED4), 0.06 (ED6), and 0.08/h (ED8). Hybrids exhibited a range in whole plant DM content (23.7 to 25.0%), starch content (15.9 to 28.1% DM), kernel hardness (21.9 to 34.4 s/20 g) and density (3.57 to 4.18 g/mL), and prolamin content (8.24 to 11.34 g/100 g starch). Differences in digestion kinetics among hybrids were generally more pronounced for starch than DM. The hybrids differed in starch degradability (P &lt; 0.05), with earlier maturing hybrids having lower A fraction, lower kd, and lower ED, with hybrid effects on ED being accentuated with faster passage rate. Kernel DM content (r = −0.85, −0.87), hardness (r = −0.89, −0.86), and density (r = −0.84, −0.85) were negatively correlated with ED4 and ED8 of starch, respectively, due mainly to decreased kd of fraction B. Reducing the particle size of kernels increased ED of starch due to increased A fraction and kd of the B fraction. A tendency (P = 0.09) for hybrid × processing effects for ED6 and ED8 indicated that processing had greater effects on increasing ED of starch for earlier maturing hybrids. We conclude that short-season hybrids that mature early may have lower ED of DM and starch and would benefit from prolonged ensilage time. Kernel processing during silage making is recommended for short-season corn hybrids as a means of enhancing rumen availability of starch.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 27-28
Author(s):  
Vitor R G Mercadante ◽  
Robin R White ◽  
John Currin ◽  
Heather L Bradford ◽  
Sherrie Clarke ◽  
...  

Abstract The objective of this study was to establish the relationships between when a cow was born within the calving season and the performance of her offspring. Data from the Virginia Department of Corrections beef cattle herds were collated for 7 locations over 7 years (2010 to 2017), with 2 calving seasons per year. Data from Spring of 2011 were missing. The full dataset contained 19,253 individual animal observations. Within each location, year, and calving season combination, the calving season was defined as starting when the first calf was born and terminating when the final calf was born. The relative calving date (RCD) within each calving season was defined by calculating the number of days between when the calving occurred and when the calving season started, divided by the length of the season in days. For heifer calves that were retained as replacement heifers (n = 2,800), the RCD and birth weight of their calves were used as response variables of a mixed-effect model with fixed effects of dam RCD, season (fall vs spring), and calf sex. All 2 and 3 way interactions were also included as fixed effects. Both calving year and sire were used as random effects. Fixed effects were iteratively removed from the model when non-significant; however, non-significant linear terms were retained if involved in a significant interaction term. The final calf RCD model included significant effects of dam RCD (P = 0.006), season (P &lt; 0.001), calf sex (P = 0.0737), and the interaction between dam RCD and calf sex (P = 0.055). The final calf birth weight model included only linear terms for calf sex (P &lt; 0.001) and dam RCD (P = 0.029). Least square means for these relationships are depicted in Table 1.


1989 ◽  
Vol 69 (4) ◽  
pp. 973-987 ◽  
Author(s):  
MARINA A. G. VON KEYSERLINGK ◽  
G. W. MATHISON

The in situ bag technique was evaluated as a method of predicting the voluntary intake and digestibility of forages. Nine feedstuffs including three cereal straws, three alfalfa (Medicago sativa L.) and grass hay mixtures, two alfalfa-grass silages, and a prairie (Festuca hallii L.) grass hay were each fed to five steers (350 ± 49.5 kg) at ad libitum and approximately maintenance feeding levels. Hays and silages were also fed at near the twice maintenance feeding level. Voluntary forage dry matter (DM) intakes ranged from 1.00 to 2.85% of body weight. Apparent forage DM digestibilities ranged from 37 to 64%. At the lower feeding level fractional rates of particulate passage through the digestive tract were higher (P < 0.05) for 37% DM silage (0.037 h−1) than for two of the straw-based diets (0.020–0.023 h−1). Particulate passage rates were not (P > 0.05) affected by feeding level. Predictions from in situ results were most accurate when 24 and 36 h degradation values were used to estimate DM digestibility (R2 = 0.92) and intake (R2 = 0.86), respectively. The neutral detergent fiber content of forage was equally well correlated with DM digestibility (R2 = 0.86) and voluntary DM intake (R2 = 0.85). Inclusion of information on rates of passage in prediction equations did not (P > 0.05) improve accuracies of predictions over those obtained using in situ results or chemical feed components. Key words: Steers, in situ, voluntary intake, digestibility, passage rates, prediction


2021 ◽  
Vol 51 (5) ◽  
Author(s):  
Gabriel Santos Persiquini Cunha ◽  
Moises de Aguiar Maia ◽  
Luís Miguel Gonçalves Fernandes ◽  
Luciana Castro Geraseev ◽  
Amália Saturnino Chaves

ABSTRACT: This study evaluated the effects of the physically effective fiber (peNDF) content on nutrient composition of the total mixed ration (TMR), orts and fecal parameters in 15 commercial beef feedlots. The particle size distribution of TMR was measured adopting Penn State Particle Size Separator (PSPS). Samples were evaluated for dry matter (DM), ash, ether extract (EE), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and starch content. Fecal samples were also evaluated for pH and score. The feedlots were categorized into peNDF<20% and peNDF ≥ 20%. Data were analyzed by a model that included fixed effects of treatment and covariates (sex and genetic groups). Levels of DM, CP, EE and starch were lower in TMR with peNDF ≥ 20% (P<0.05). Fecal dry matter was higher and morning fecal pH was lower for TMR with peNDF<20% (P<0.05). Physically effective fiber changed nutrient levels in TMR, orts, fecal pH and DM. So, using PSPS and fecal parameters are practical and indirect tools for measuring fecal starch and assessing the effectiveness of the diet supplied to ruminants.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 174-174
Author(s):  
Raul Rivera-Chacon ◽  
Sara Ricci ◽  
Nicole Reisinger ◽  
Qendrim Zebeli ◽  
Ezequias Castillo-Lopez

Abstract Modern dairy production systems require larger inputs of energy in diets to increase milk yield. Therefore, dairy cows are at risk of experiencing subacute ruminal acidosis (SARA). The objective of this study was to evaluate grains and forage rumen degradability in cows fed different diets supplemented with a phytogenic (PHY) feed additive. The experiment was conducted with nine rumen-cannulated non-lactating Holstein cows blocked in two groups of four and five animals, and were part of a cross-over design. This study consisted in 2 runs separated by a 12-week washout period, in which cows grazed on pasture. Each experimental run had one week of forage (F) feeding, one week of transition to a high grain (HG) diet, and four weeks of HG (65:35 concentrate to forage ratio in dry matter basis). Cows were supplemented with PHY (a blend of menthol and thymol) or a control carrier (CON) from week F. Corn and wheat grain were ground through a 4 mm screen, while grass silage was ground through a 6 mm screen. Sampling for in situ degradability was performed in F and in week four of HG diet. Data were analyzed with SAS with week of feeding and supplementation as fixed effects and cow as random effect. Corn and wheat 24-h dry matter (DM) and organic matter (OM) degradability increased during HG diet compared with F (P &lt; 0.05), grass silage DM and OM 48-h degradability was reduced by HG diet compared to F (P &lt; 0.01). Additionally, wheat grain 24-h OM degradability was higher for PHY compared to CON under F and HG diet conditions (P = 0.05). Overall, diet composition and SARA conditions can influence grains and fiber degradability in the rumen.


2003 ◽  
Vol 105 (1-4) ◽  
pp. 185-198 ◽  
Author(s):  
N.W Galdámez-Cabrera ◽  
K.P Coffey ◽  
W.K Coblentz ◽  
J.E Turner ◽  
D.A Scarbrough ◽  
...  

2002 ◽  
Vol 82 (2) ◽  
pp. 207-213 ◽  
Author(s):  
H. W. Soita ◽  
D. A. Christensen ◽  
J. J. McKinnon ◽  
A. F. Mustafa

Two studies were conducted to determine the effects of two theoretical lengths of cut (TLC) (short = 4.7 mm and long = 18.8 mm) on the feeding value of barley silage to steers fed an all-forage diet. In the first study, six steers were used to determine intake of dry matter, total tract digestibility of dry matter (DMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) in a replicated switchback design. In the second study, six steers equipped with rumen cannulas were used to determine the effects of barley silage particle size on ruminal kinetic and fermentation parameters. Feeding short barley silage to steers increased (P < 0.05) dry matter intake (DMI), DMD, NDFD, and ADFD compared with feeding long barley silage. The results of the second study showed that reducing barley silage particle size decreased (P < 0.05) ruminal retention time and increased (P < 0.05) passage rate of particulate matter. Ruminal pH was lower (P < 0.05) at 1000, 1200, 1800, 2000, and 2200 h for steers fed the short barley silage compared with those fed the long barley silage. However, the effects of barley silage particle size on ruminal ammonia nitrogen and volatile fatty acid (VFA) concentrations were minimal. Eating rate was similar in both dietary treatments (average 40.3 g min-1). However, steers fed the long silage produced more (P < 0.05) saliva per kg DMI compared with those fed the short barley silage diets. It was concluded that reducing TLC of barley silage increased feed intake and improved total tract utilization of cell wall components by steers. Results of the metabolism study also showed changes in fermentation parameters, but no adverse physiological changes were noted when cattle were fed all forage diets. Key words: Barley silage, particle size, fermentation patterns


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