scholarly journals An Examination of Flying Insects in Seven Hospitals in the United Kingdom and Carriage of Bacteria by True Flies (Diptera: Calliphoridae, Dolichopodidae, Fanniidae, Muscidae, Phoridae, Psychodidae, Sphaeroceridae)

2019 ◽  
Vol 56 (6) ◽  
pp. 1684-1697 ◽  
Author(s):  
Federica Boiocchi ◽  
Matthew P Davies ◽  
Anthony C Hilton

AbstractInsects are efficient vectors of bacteria and in the hospital environment may have a role in spreading nosocomial infections. This study sampled the flying insect populations of seven hospitals in the United Kingdom and characterized the associated culturome of Diptera, including the antibiotic resistance profile of bacterial isolates. Flying insects were collected in seven U.K. hospitals between the period March 2010 to August 2011. The bacteria carried by Diptera were isolated using culture-based techniques, identified and characterized by antimicrobial susceptibility testing. A total of 19,937 individual insects were collected with Diptera being the most abundant (73.6% of the total), followed by Hemiptera (13.9%), Hymenoptera (4.7%), Lepidoptera (2.9%), and Coleoptera (2%). From Diptera, 82 bacterial strains were identified. The majority of bacteria belonged to the Enterobacteriaceae (42%), followed by Bacillus spp. (24%) and Staphylococcus spp. (19%). Less abundant were bacteria of the genus Clostridium (6%), Streptococcus (5%), and Micrococcus (2%). A total of 68 bacterial strains were characterized for their antibiotic resistance profile; 52.9% demonstrated a resistant phenotype to at least one class of antibiotic. Staphylococcus spp. represented the highest proportion of resistant strains (83.3%), followed by Bacillus spp. (60%) and Enterobacteriaceae (31.3%). Diptera were the predominant flying insects present in the U.K. hospital environments sampled and found to harbor a variety of opportunistic human pathogens with associated antimicrobial resistance profiles. Given the ability of flies to act as mechanical vectors of bacteria, they present a potential to contribute to persistence and spread of antimicrobial-resistant pathogenic bacteria in the hospital environment.

Author(s):  
Elhassan Benyagoub ◽  
Nouria Nabbou ◽  
Dalila Razni ◽  
Snoussi Moghtet

Objective: The purpose of this work is to study the biological activity of Anastatica hierochuntica L., against nine bacterial strains responsible for women’s uro-genital infection (UGI).Methods: The plant was collected from Tindouf region (far southwest Algeria). In this study, we performed an evaluation of antibacterial activity of three macerates of two vegetative parts (seeds and stems) by two methods (disc and wells diffusion methods), with a description of the antibiotic resistance profile of isolated bacterial strains by antibiogram method.Results: According to the results, the antibiotic resistance profile of the tested bacterial strains showed an increased resistance against several antibiotics families. The evaluation of the antibacterial potential of macerates showed that methanolic and aqueous macerates of the seeds were more active against Gram-positive bacteria compared to Gram-negative bacteria.Conclusion: The preliminary results of this study allowed us to predict that natural substances in the plant can be considered as an important source to possess compounds with significant antibacterial properties and thus suggest their application in the pharmaceutical industry.


Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 575
Author(s):  
Emi Nishimura ◽  
Masateru Nishiyama ◽  
Kei Nukazawa ◽  
Yoshihiro Suzuki

Information on the actual existence of antibiotic-resistant bacteria in rivers where sewage, urban wastewater, and livestock wastewater do not load is essential to prevent the spread of antibiotic-resistant bacteria in water environments. This study compared the antibiotic resistance profile of Escherichia coli upstream and downstream of human habitation. The survey was conducted in the summer, winter, and spring seasons. Resistance to one or more antibiotics at upstream and downstream sites was on average 18% and 20%, respectively, and no significant difference was observed between the survey sites. The resistance rates at the upstream site (total of 98 isolated strains) to each antibiotic were cefazolin 17%, tetracycline 12%, and ampicillin 8%, in descending order. Conversely, for the downstream site (total of 89 isolated strains), the rates were ampicillin 16%, cefazolin 16%, and tetracycline 1% in descending order. The resistance rate of tetracycline in the downstream site was significantly lower than that of the upstream site. Furthermore, phylogenetic analysis revealed that many strains showed different resistance profiles even in the same cluster of the Pulsed-Field Gel Electrophoresis (PFGE) pattern. Moreover, the resistance profiles differed in the same cluster of the upstream and the downstream sites. In flowing from the upstream to the downstream site, it is plausible that E. coli transmitted or lacked the antibiotic resistance gene.


Author(s):  
Anupama Bhardwaj ◽  
Jagtar Singh ◽  
Sonia Chaman ◽  
Amit Joshi

Objective: The objective of this study is to make sure biotreatment process used for treatment of dairy wastewater (DWW) is safe for human and its surrounding environment; microbes were evaluated for their antibiotic resistance profile against commonly prescribed antibiotics. Methods: Microbes were isolated using spread plating and streaking method and used to treat DWW. Reduction in organic load in DWW was determined by comparing physicochemical parameters (PCP) of DWW before and after treatment process. After selection of efficient microbial isolates, they were evaluated for their antibiotic resistance profile using antibiotic disc diffusion method. Results: In this work, 53 microbes were isolated from DWW, and these microbial isolates were screened for DWW degradation capacity by analyzing PCP. Four microbial isolates E3, E5, E11 (bacterial isolates) and F5 (fungal isolate) showed highest reduction in chemical oxygen demand (COD), biological oxygen demand (BOD), and dissolved oxygen (DO) were selected for profound degradation of DWW under optimized conditions. Efficient four microbial isolates individually performed better under anaerobic conditions by showing maximum reduction 84%, 75%, and 77% in COD, BOD, and DO, respectively. After 72 hrs of antibiotic susceptibility testing, E3 strain had shown 100%, E5 90%, E11 70%, and F5 80% susceptibility to antibiotics. Conclusion: The present study concluded that four microbial isolates had the potential of reducing the organic load of DWW along with lessor or negligible adverse effect on human or its surrounding environment and they appear to be most promising strains for treatment of DWW. 


2021 ◽  
Vol 09 (11) ◽  
pp. 52-67
Author(s):  
Chinedum Taahie Oparaugo ◽  
Bamidele Abiodun Iwalokun ◽  
Adesegun Abiola Adesesan ◽  
Idowu Olaide Edu-Muyideen ◽  
Abimbola Modupe Adedeji ◽  
...  

2021 ◽  
pp. 2219-2229
Author(s):  
Prudence Mpundu ◽  
Allan Rabson Mbewe ◽  
John Bwalya Muma ◽  
Wizaso Mwasinga ◽  
Nawa Mukumbuta ◽  
...  

Background and Aim: Listeria monocytogenes in ready-to-eat (RTE) foods remains consistently under-reported globally. Nevertheless, several independent studies conducted to investigate have elucidated the prevalence and antibiotic resistance profiles of L. monocytogenes in RTE-associated foods and their antibiotic resistance profiles. Given the rapid increase in consumption of RTE foods of both animal and plant origin, it is imperative to know the prevalence deductive data focusing on how much of L. monocytogenes is present in RTE foods, which is critical for food safety managers and retailers to assess the possible risk posed to end-users. In addition, valuable insight and another angle to the depth of the problem, we conducted a systematic review and meta-analysis to synthesize available data regarding the prevalence of L. monocytogenes in RTE foods and antibiotic resistance profiles. Materials and Methods: We conducted a meta-analysis study of L. monocytogenes and antibiotic resistance to clinically relevant antibiotics to determine the extent of L. monocytogenes contamination in RTE foods and antibiotic resistance profiles. The primary search terms, also known as keywords used, were restricted to peer-reviewed and review articles, and databases, including Google Scholars, Science-Direct, and Scopus, were searched. The inclusion of articles meeting eligibility criteria published between 2010 and 2020 after title, abstract, and full article screening. Data analysis was performed at multiple stages using quantitative meta-analysis reviews. Results: L. monocytogenes pooled proportion/prevalence was highest in chicken products determined at (22%) followed by various but uncategorized RTE foods at 21%. Regarding antibiotic resistance, profiling's highest pooled prevalence resistance was observed in penicillin at 80% resistance, followed by cephalosporin at 47%. Conclusion: Within its limitations, this study has attempted to provide insight into the pooled proportion/prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile at the global level. Determining the proportion/ prevalence of L. monocytogenes in RTE foods across the globe and antibiotic resistance profile is essential for providing quality food and reducing public health problems due to unsuccessful treatment of foodborne illness. This study provides insight into the pooled prevalence of L. monocytogenes in RTE foods and the antibiotic resistance profile. The results of this study partly endeavored to help appropriate authorities strengthen their preventive measures on specific RTE foods that are most likely to be contaminated with L. monocytogenes and antibiotic resistance profiles.


2018 ◽  
Vol 27 (4) ◽  
pp. 1107-1111 ◽  
Author(s):  
Nasrin Askari ◽  
Amir Saeed Samimi ◽  
Reza Kheirandish ◽  
Reza Ghanbarpour ◽  
Saeed Hasanzadeh ◽  
...  

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