scholarly journals Expression of a putative high-affinity NO3- transporter and of an H+-ATPase in relation to whole plant nitrate transport physiology in two maize genotypes differently responsive to low nitrogen availability

2003 ◽  
Vol 54 (384) ◽  
pp. 1023-1031 ◽  
Author(s):  
S. Quaggiotti ◽  
B. Ruperti ◽  
P. Borsa ◽  
T. Destro ◽  
M. Malagoli
Keyword(s):  
Nitrogen Availability ◽  
Nitrate Transport ◽  
High Affinity ◽  
Maize Genotypes ◽  
Whole Plant ◽  
Low Nitrogen

scholarly journals GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and -independent pathways

2021 ◽  
Author(s):  
Alaeddine Safi ◽  
Anna Medici ◽  
Wojciech Szponarski ◽  
Florence Martin ◽  
Anne Clément-Vidal ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Signaling Pathway ◽  
Nitrogen Availability ◽  
Nitrogen Starvation ◽  
Transport Systems ◽  
Nitrate Transport ◽  
Molecular Physiology ◽  
Starvation Response ◽  
High Affinity ◽  
N Remobilization

Abstract Plants need to cope with strong variations of nitrogen availability in the soil. Although many molecular players are being discovered concerning how plants perceive NO3− provision, it is less clear how plants recognize a lack of nitrogen. Following nitrogen removal, plants activate their nitrogen starvation response (NSR), which is characterized by the activation of very high-affinity nitrate transport systems (NRT2.4 and NRT2.5) and other sentinel genes involved in N remobilization such as GDH3. Using a combination of functional genomics via transcription factor perturbation and molecular physiology studies, we show that the transcription factors belonging to the HHO subfamily are important regulators of NSR through two potential mechanisms. First, HHOs directly repress the high-affinity nitrate transporters, NRT2.4 and NRT2.5. hho mutants display increased high-affinity nitrate transport activity, opening up promising perspectives for biotechnological applications. Second, we show that reactive oxygen species (ROS) are important to control NSR in wild-type plants and that HRS1 and HHO1 overexpressors and mutants are affected in their ROS content, defining a potential feed-forward branch of the signaling pathway. Taken together, our results define the relationships of two types of molecular players controlling the NSR, namely ROS and the HHO transcription factors. This work (i) up opens perspectives on a poorly understood nutrient-related signaling pathway and (ii) defines targets for molecular breeding of plants with enhanced NO3− uptake.

scholarly journals A Reevaluation of the Role of Arabidopsis NRT1.1 in High-Affinity Nitrate Transport

2013 ◽  
Vol 163 (3) ◽  
pp. 1103-1106 ◽  
Author(s):  
Anthony D.M. Glass ◽  
Zorica Kotur
Keyword(s):  
Nitrate Transport ◽  
High Affinity

Regulation of the High-Affinity Nitrate Transport System in Wheat Roots by Exogenous Abscisic Acid and Glutamine

2007 ◽  
Vol 49 (12) ◽  
pp. 1719-1725 ◽  
Author(s):  
Chao Cai ◽  
Xue-Qiang Zhao ◽  
Yong-Guan Zhu ◽  
Bin Li ◽  
Yi-Ping Tong ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Transport System ◽  
Nitrate Transport ◽  
Wheat Roots ◽  
High Affinity

scholarly journals Corrigendum to: A high affinity nitrate transport system from Chlamydomonas requires two gene products (FEBS 23233)

FEBS Letters ◽  
2000 ◽  
Vol 481 (1) ◽  
pp. 88-88
Author(s):  
Jing-Jiang Zhou ◽  
Emilio Fernández ◽  
Aurora Galván ◽  
Anthony J. Miller
Keyword(s):  
Transport System ◽  
Nitrate Transport ◽  
Gene Products ◽  
High Affinity

scholarly journals Influence of Genotype, Site, and Year on Maize Nutritive Value – Yield Relationships

2017 ◽  
Vol 48 (2) ◽  
pp. 47-53
Author(s):  
J. Hakl ◽  
R. Loučka ◽  
J. Jirmanová ◽  
V. Jambor
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Maize Yield ◽  
Matter Content ◽  
Forage Yield ◽  
Dry Matter Content ◽  
Yield And Quality ◽  
Maize Genotypes ◽  
Whole Plant ◽  
Maize Genotype

Abstract Maize genotype selection represents a practical tool influencing forage yield and quality. The main objective was to investigate the contribution of genotype, site, and year to variability of maize yield and quality in the environment of Central Europe. Totally 63 maize genotypes at 11 sites over a 7-year period were evaluated for dry matter yield (DMY), dry matter content (DM), starch, cob, neutral detergent fibre (NDF), in situ digestibility of stover NDF (NDFD), and organic matter (OMD). The genotype showed the highest variability from all factors where stover NDFD varied from 261 to 529 g kg-1 and stover OMD from 376 to 609 g kg-1. In contrast to the whole-plant, variability of stover traits was more closely related to NDF than the DM content. Under standardized plant DM, all tested factors were significant and allowed interpretation of 70 and 60% of total variation of yield and quality for stover and whole plant, respectively. The average contributions of genotype, site, and year were 30, 7, and 5%, respectively. For variability in plant productivity and nutritive value, the importance of maize genotype selection was more than two times higher than the contribution of environment.

scholarly journals Phylogenomic and Microsynteny Analysis Provides Evidence of Genome Arrangements of High-Affinity Nitrate Transporter Gene Families of Plants

2021 ◽  
Vol 22 (23) ◽  
pp. 13036
Author(s):  
Normig M. Zoghbi-Rodríguez ◽  
Samuel David Gamboa-Tuz ◽  
Alejandro Pereira-Santana ◽  
Luis C. Rodríguez-Zapata ◽  
Lorenzo Felipe Sánchez-Teyer ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Nitrate Assimilation ◽  
Gene Families ◽  
Land Plants ◽  
Nitrate Transport ◽  
Phylogenomic Analysis ◽  
Evolutionary Constraint ◽  
N Availability ◽  
Nitrate Transporter ◽  
High Affinity

Nitrate transporter 2 (NRT2) and NRT3 or nitrate-assimilation-related 2 (NAR2) proteins families form a two-component, high-affinity nitrate transport system, which is essential for the acquisition of nitrate from soils with low N availability. An extensive phylogenomic analysis across land plants for these families has not been performed. In this study, we performed a microsynteny and orthology analysis on the NRT2 and NRT3 genes families across 132 plants (Sensu lato) to decipher their evolutionary history. We identified significant differences in the number of sequences per taxonomic group and different genomic contexts within the NRT2 family that might have contributed to N acquisition by the plants. We hypothesized that the greater losses of NRT2 sequences correlate with specialized ecological adaptations, such as aquatic, epiphytic, and carnivory lifestyles. We also detected expansion on the NRT2 family in specific lineages that could be a source of key innovations for colonizing contrasting niches in N availability. Microsyntenic analysis on NRT3 family showed a deep conservation on land plants, suggesting a high evolutionary constraint to preserve their function. Our study provides novel information that could be used as guide for functional characterization of these gene families across plant lineages.

scholarly journals An Integrated Analysis of the Rice Transcriptome and Metabolome Reveals Root Growth Regulation Mechanisms in Response to Nitrogen Availability

2019 ◽  
Vol 20 (23) ◽  
pp. 5893 ◽  
Author(s):  
Wei Xin ◽  
Lina Zhang ◽  
Wenzhong Zhang ◽  
Jiping Gao ◽  
Jun Yi ◽  
...  
Keyword(s):  
Root Growth ◽  
Differentially Expressed Genes ◽  
Nitrogen Availability ◽  
Rice Root ◽  
Integrated Analysis ◽  
High Nitrogen ◽  
Rice Roots ◽  
Regulation Mechanisms ◽  
Low Nitrogen ◽  
Nitrogen Conditions

Nitrogen is an essential nutrient for plant growth and basic metabolic processes. Root systems play an important role in the ability of plants to obtain nutrients from the soil, and are closely related to the growth and development of above-ground plants. Root morphology analysis showed that root growth was induced under low-nitrogen conditions and inhibited under high-nitrogen conditions. To better understand the molecular mechanisms and metabolic basis underlying the rice root response to nitrogen availability, an integrated analysis of the rice root transcriptome and metabolome under three environmental conditions (low-, control, and high-nitrogen conditions) was conducted. A total of 262 and 262 differentially level metabolites were identified under low- and high-nitrogen conditions, respectively. A total of 696 and 808 differentially expressed genes were identified under low- and high-nitrogen conditions, respectively. For both the differentially expressed genes and metabolites, KEGG pathway analysis indicated that amino acid metabolism, carbon and nitrogen metabolism, phenylpropanoid metabolism, and phytohormones’ signal transduction were significantly affected by nitrogen availability. Additionally, variable levels of 65 transcription factors (TFs) were identified in rice leaves exposed to high and low nitrogen, covering 22 TF families. These results also indicate that there is a significant difference in the transcriptional regulation mechanisms of rice roots between low and high nitrogen. In summary, our study provides new information for a further understanding of the response of rice roots to low-nitrogen and high-nitrogen conditions.

Molecular biology of inducible high-affinity nitrate transport in higher plants

2001 ◽  
pp. 12-13
Author(s):  
G. Leggewie ◽  
B. G. Forde ◽  
K. Piepenburg ◽  
M. Udvardi
Keyword(s):  
Molecular Biology ◽  
Higher Plants ◽  
Nitrate Transport ◽  
High Affinity

scholarly journals The Role of Gibberellins in Regulation of Nitrogen Uptake and Physiological Traits in Maize Responding to Nitrogen Availability

2020 ◽  
Vol 21 (5) ◽  
pp. 1824
Author(s):  
Yubin Wang ◽  
Qingqing Yao ◽  
Yushi Zhang ◽  
Yuexia Zhang ◽  
Jiapeng Xing ◽  
...  
Keyword(s):  
Nitrogen Availability ◽  
Physiological Responses ◽  
N Uptake ◽  
Nitrogen Supply ◽  
Rna Seq ◽  
Uptake Capacity ◽  
Use Efficiency ◽  
Low Nitrogen ◽  
N Use

Modified gibberellin (GA) signaling leads to semi-dwarfism with low nitrogen (N) use efficiency (NUE) in crops. An understanding of GA-mediated N uptake is essential for the development of crops with improved NUE. The function of GA in modulating N uptake capacity and nitrate (NO3−) transporters (NRTs) was analyzed in the GA synthesis-deficient mutant zmga3ox grown under low (LN) and sufficient (SN) N conditions. LN significantly suppressed the production of GA1, GA3, and GA4, and the zmga3ox plants showed more sensitivity in shoots as well as LN stress. Moreover, the higher anthocyanin accumulation and the decrease of chlorophyll content were also recorded. The net NO3− fluxes and 15N content were decreased in zmga3ox plants under both LN and SN conditions. Exogenous GA3 could restore the NO3− uptake in zmga3ox plants, but uniconazole repressed NO3− uptake. Moreover, the transcript levels of ZmNRT2.1/2.2 were downregulated in zmga3ox plants, while the GA3 application enhanced the expression level. Furthermore, the RNA-seq analyses identified several transcription factors that are involved in the GA-mediated transcriptional operation of NRTs related genes. These findings revealed that GAs influenced N uptake involved in the transcriptional regulation of NRTs and physiological responses in maize responding to nitrogen supply.

Sign in / Sign up

Export Citation Format

Share Document