Haematology

2010 ◽  
pp. 211-308 ◽  
Keyword(s):  
Platelet Count ◽  
Blood Cell ◽  
Cell Morphology ◽  
Blood Count ◽  
Iron Status ◽  
Blood Film ◽  
Full Blood Count ◽  
Red Cell ◽  
Cell Parameters ◽  
Blood Cell Morphology

Full blood count 212 Red cell parameters 214 White cells 216 Platelet count 217 Peripheral blood film 218 Red cell morphology 219 Parasites on the blood film & marrow 222 White blood cell morphology 224 Assessment of iron status 226 Assessment of vitamin B12...

scholarly journals The stability of sample storage for complete blood count (CBC) toward the blood cell morphology

2019 ◽  
Vol 8 (2) ◽  
pp. 482
Author(s):  
Arie Rahmanitarini ◽  
Yetti Hernaningsih ◽  
Yulia Nadar Indrasari
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Blood Count ◽  
Complete Blood Count ◽  
Sample Storage ◽  
Blood Cell Morphology ◽  
The Stability

scholarly journals Full blood count as potential predictor of outcomes in patients undergoing cardiac resynchronization therapy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nikolaos Papageorgiou ◽  
Debbie Falconer ◽  
Adam Ioannou ◽  
Tanakal Wongwarawipat ◽  
Sergio Barra ◽  
...  
Keyword(s):  
Platelet Count ◽  
Cardiac Resynchronization Therapy ◽  
Blood Count ◽  
Cardiac Resynchronization ◽  
Full Blood Count ◽  
Red Cell ◽  
Resynchronization Therapy ◽  
Lv Remodeling ◽  
All Cause Mortality

Abstract Almost a third of patients fulfilling current guidelines criteria have suboptimal responses following cardiac resynchronization therapy (CRT). Circulating biomarkers may help identify these patients. We aimed to assess the predictive role of full blood count (FBC) parameters in prognosis of heart failure (HF) patients undergoing CRT device implantation. We enrolled 612 consecutive CRT patients and FBC was measured within 24 hours prior to implantation. The follow-up period was a median of 1652 days (IQR: 837–2612). The study endpoints were i) composite of all-cause mortality or transplant, and ii) reverse left ventricular (LV) remodeling. On multivariate analysis [hazard ratio (HR), 95% confidence interval (CI)] only red cell count (RCC) (p = 0.004), red cell distribution width (RDW) (p < 0.001), percentage of lymphocytes (p = 0.03) and platelet count (p < 0.001) predicted all-cause mortality. Interestingly, RDW (p = 0.004) and platelet count (p = 0.008) were independent predictors of reverse LV remodeling. This is the first powered single-centre study to demonstrate that RDW and platelet count are independent predictors of long-term all-cause mortality and/or heart transplant in CRT patients. Further studies, on the role of these parameters in enhancing patient selection for CRT implantation should be conducted to confirm our findings.

A Comprehensive Report of Blood Cell Morphology for Neonates with Down's Syndrome In the UK: A Report From the Children with Down's Syndrome Study

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 80-80
Author(s):  
Rebecca James ◽  
Tom Johnston ◽  
Tracy Lightfoot ◽  
Dan Painter ◽  
Pat Ansell ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Population Based ◽  
Full Blood Count ◽  
Target Cells ◽  
Cell Type ◽  
Giant Platelets ◽  
Blood Cell Morphology

Abstract Abstract 80 Introduction: The Children with Down's Syndrome Study (CDSS; www.cdss.org.uk) is a unique observational study following a population-based cohort of children with Down's syndrome (DS) from birth onwards. Although haematological abnormalities are well recognised in DS neonates blood morphology is less well described. This is the first prospective report of blood cell morphology in DS neonates. Methods: Recruitment to the population based CDSS opened in May 2006 and is ongoing. At entry, a blood sample taken in EDTA is sent to a designated laboratory with full CPA (UK) accreditation. Blood films were reviewed by two Paediatric Haematologists following a specific proforma developed after a series of pilot studies which had identified the range of morphological abnormalities seen in DS neonates. Reporting was subsequently standardised so that inter and intra-operator variability were minimised. Critically, abnormal features were recorded for each individual white cell. All slides reported on here were taken within 28 days of birth and had to be of adequate quality with regard to stain, spread and EDTA change. These were considered independently for cell type. Results: Microscopic review was performed in slides from 154 DS neonates; 88 of these met the quality criteria for at least one cell type. The most consistent abnormalities were of platelets: giant platelets were present in 78/88 (89%); pale, hypogranular platelets in 61/88 (69%) and megakaryocyte fragments in 30/88 (34%). The most common red blood cell changes were macrocytosis and polychromasia, occurring in ≥5% all red cells in 48/68 (71%) and 33/68 (49%) respectively. Nucleated red blood cells were seen in 34/71 (48%); spherocytes in 28/68 (41%); target cells in 27/68 (40%); Howell Jolly bodies in 26/68 (38%) and basophilic stippling in 9/69 (13%). The neutrophils tended to be hypogranular 35/36 (97%) and/or agranular 6/36 (17%). None were hypergranular. Neutrophil vacuolation was common, occurring in 29/36 (81%) whilst Pelgeroid neutrophils occurred in 12/36 (33%) and hypersegmentation in 7/36 (19%). Granulocytic nuclear/cytoplasmic dysynchrony was noted in 25/36 (69%) with the nucleus appearing relatively immature and with the granules often clustering in one area leaving an area of agranular bluish cytoplasm. Lymphocyte morphology was unremarkable. Monocytes were typically vacuolated and often had stellate or elongated nuclei: these features occurred in 30/36 (83%); 27/36 (75%) and 6/36 (17%) slides respectively; no azurophilic granules were seen. Both eosinophils and basophils tended to be dysplastic with abnormal hypogranulation in 18/36 (50%) and 20/36 (56%) respectively. Blasts were seen in 19/36 (53%), but cytoplasmic blebbing was rare 2/36 (6%). In the majority the blast type could not be ascribed. Conclusions: The blood cell morphology of DS neonates described above is distinctive and resembles that typically seen in the fetus. An unusual feature of DS neonates is the spontaneous regression of transient myeloproliferative disorder (TMD). This work suggests that fetal, ie hepatic, haematopoiesis is still significant in DS neonates and supports the idea that TMD arises from a fetal progenitor and regresses as the cellular context changes to turn off hepatic haematopoiesis. Myeloid leukaemia later develops in ∼20% who have had TMD. This might occur if a GATA1 mutated fetal progenitor was able to seed and successfully populate the bone marrow. Importantly, although blasts were present in 53%, not all of these had TMD. There is a clear clinical imperative for identifying TMD as it occurs in ∼4-6% DS neonates and defines a group at high risk of developing a highly treatable leukaemia within a limited time-frame. This work suggests that full blood count and film review alone are insufficient to identify TMD, and that GATA1 mutation analysis is needed. Such expensive, time consuming and specialized analysis would require a national approach. An important lesson of this work is also the need to standardise morphology reporting: to quantify what is an inherently qualitative and subjective process. The proforma developed here provides a template for others to use in this group and in the general population. Disclosures: No relevant conflicts of interest to declare.

scholarly journals The Effects of Sample Transport by Pneumatic Tube System on Routine Hematology and Coagulation Tests

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Devi Subbarayan ◽  
Chidambharam Choccalingam ◽  
Chittode Kodumudi Anantha Lakshmi
Keyword(s):  
Platelet Count ◽  
Blood Count ◽  
Complete Blood Count ◽  
Red Cell ◽  
Tube System ◽  
Cell Parameters ◽  
Pneumatic Tube ◽  
Paired Samples ◽  
Significant Difference ◽  
Pneumatic Tube System

Background. Automation helps improve laboratory operational efficiency and reduce the turnaround time. Pneumatic tube systems (PTS) automate specimen transport between the lab and other areas of the hospital. Its effect on complete blood count (CBC) and coagulation is still controversial.Aim. To study the effects of pneumatic tube system sample transport on complete blood count and coagulation parameters to compare them with hand delivered samples.Methods. 75 paired samples for complete blood count and 25 paired samples for coagulation analysis were compared between samples sent via pneumatic tube system and hand delivered system.Results. PTS showed significant decrease in red cell indices such as MCV and RDW and increase in MCHC. Other red cell parameters and WBC parameters showed no statistical significant difference. Statistically significant increase in platelet count was observed with PTS samples. However, these differences were clinically insignificant. No significant effect of PTS was found in PT and APTT samples compared to the hand delivered samples.Conclusion. Despite statistically significant changes in RBC parameters such as MCV, RDW, and MCHC and platelet count, these changes were clinically insignificant. Hence, blood samples for CBC and coagulation assay can safely be transported via our hospital’s PTS. However, further studies on platelet count are warranted to ensure safe transport and accuracy of the results.

scholarly journals Iron alters red blood cell morphology

Blood ◽  
2013 ◽  
Vol 121 (1) ◽  
pp. 9-9 ◽  
Author(s):  
Etheresia Pretorius ◽  
Boguslaw Lipinski
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Red Blood Cell ◽  
Blood Cell Morphology

scholarly journals Prevalence and aetiologies of anaemia among first trimester pregnant women in Sri Lanka; the need for revisiting the current control strategies

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Gayani Shashikala Amarasinghe ◽  
Thilini Chanchala Agampodi ◽  
Vasana Mendis ◽  
Krishanthi Malawanage ◽  
Chamila Kappagoda ◽  
...  
Keyword(s):  
Public Health ◽  
Iron Deficiency ◽  
Pregnant Women ◽  
Blood Count ◽  
First Trimester ◽  
Full Blood Count ◽  
Red Cell ◽  
Middle Income ◽  
Anaemia In Pregnancy ◽  
In Pregnancy

Abstract Background The Sustainable development goals, which focus strongly on equity, aim to end all forms of malnutrition by 2030. However, a significant cause of intergenerational transfer of malnutrition, anaemia in pregnancy, is still a challenge. It is especially so in the low- and middle-income settings where possible context-specific aetiologies leading to anaemia have been poorly explored. This study explores the prevalence of etiological factors significantly contributing to anaemia in pregnancy in Sri Lanka, a lower-middle-income country with a high prevalence of malnutrition albeit robust public health infrastructure. Methods All first-trimester pregnant women registered in the public maternal care programme in the Anuradhapura district from July to September 2019 were invited to participate in Rajarata Pregnancy Cohort (RaPCo). After a full blood count analysis, high-performance liquid chromatography, peripheral blood film examination, serum B12 and folate levels were performed in anaemic participants, guided by an algorithm based on the red cell indices in the full blood count. In addition, serum ferritin was tested in a random subsample of 213 participants. Anaemic women in this subsample underwent B12 and folate testing. Results Among 3127 participants, 14.4% (95%CI 13.2–15.7, n = 451) were anaemic. Haemoglobin ranged between 7.4 to 19.6 g/dl. 331(10.6%) had mild anaemia. Haemoglobin ≥13 g/dl was observed in 39(12.7%). Microcytic, normochromic-normocytic, hypochromic-normocytic and macrocytic anaemia was observed in 243(54%), 114(25.3%), 80(17.8%) and two (0.4%) of full blood counts in anaemic women, respectively. Microcytic anaemia with a red cell count ≥5 * 106 /μl demonstrated a 100% positive predictive value for minor haemoglobinopathies. Minor hemoglobinopathies were present in at least 23.3%(n = 105) of anaemic pregnant women. Prevalence of iron deficiency, B12 deficiency and Southeast Asian ovalocytosis among the anaemic was 41.9% (95%CI 26.4–59.2), 23.8% (95%CI 10.6–45.1) and 0.9% (95%CI 0.3–2.3%), respectively. Folate deficiency was not observed. Conclusion Even though iron deficiency remains the primary cause, minor hemoglobinopathies, B 12 deficiency and other aetiologies substantially contribute to anaemia in pregnancy in this study population. Public health interventions, including screening for minor hemoglobinopathies and multiple micronutrient supplementation in pregnancy, should be considered in the national programme for areas where these problems have been identified.

Blood cell morphology of the Moorish gecko, Tarentola mauritanica

2007 ◽  
Vol 28 (4) ◽  
pp. 503-508 ◽  
Author(s):  
Fabio Pupin ◽  
Roberto Sacchi ◽  
Stefano Scali ◽  
Alessandra Binda ◽  
Marco Zuffi ◽  
...  
Keyword(s):  
Blood Cell ◽  
Relative Abundance ◽  
Cell Morphology ◽  
Fourth Type ◽  
Different Types ◽  
Blood Cell Morphology

Abstract The morphology of erythrocytes, trombocytes, monocytes, basophils and lymphocytes on Moorish geckos (Tarentola mauritanica) is quite similar to that of other reptiles, even though some peculiarities were detected for heterophils and eosinophils. Moreover, we found a fourth type of granulocyte whose morphology highly differs from both heterophils and eosinophils. Sexually-based differences in the relative abundance of different types of leukocytes was detected: lymphocytes were the most frequent in females, while heterophils and eosinophils prevailed in males. Interestingly, in most individuals we found intra-erythrocytic vacuoles whose structure is similar to that previously described as Chelonoplasma in tortoises and Serpentoplasma in snakes.

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